AT1G43710

emb1075 emb1075 (embryo defective 1075)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012320
Physical Location & Seq
Reverse (-)
16486230 .. 16488462
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G43710.1

Sequence Viewer

Length: 1449 bp
ATGGTTGGATCTTTGGAATCTGATCAAACTCTTTCAATGGCCACCTTAATCGAAAAACTCGACATCTTATCTGACGACTTCGATCCAACCGCCGTAGTCACCGAACCGTTACCTCCTCCGGTAACTAATGGAATCGGAGCTGATAAAGGAGGAGGAGGAGGAGAAAGAGAGATGGTTCTCGGTAGGAATATACACACAACGTCACTCGCTGTAACGGAACCGGAGGTTAACGATGAATTCACCGGAGATAAAGAAGCTTATATGGCTAGTGTTCTTGCTCGTTACCGGAAAACTTTGGTTGAACGAACCAAAAACCATTTAGGTTATCCTTATAACTTGGATTTCGACTATGGTGCGCTTGGTCAGTTACAACATTTTTCGATTAATAATCTTGGAGATCCGTTTATTGAAAGTAACTATGGTGTACACTCAAGACCTTTTGAAGTTGGTGTGTTGGATTGGTTTGCTCGTCTTTGGGAGATTGAGAGAGATGATTATTGGGGTTACATTACCAATTGTGGTACTGAAGGCAACCTTCATGGCATTTTAGTCGGGAGGGAGATGTTTCCGGATGGGATATTGTATGCGTCGCGTGAATCGCATTACTCGGTGTTTAAAGCTGCTCGAATGTATCGAATGGAGTGTGAGAAGGTTGATACGCTTATGTCAGGGGAGATTGATTGTGATGATTTGAGGAAGAAGTTGTTGGCTAATAAAGATAAACCGGCGATTCTTAATGTTAACATAGGAACGACGGTTAAAGGAGCTGTTGATGATCTTGACCTTGTTATCAAAACTCTTGAAGAGTGTGGTTTCTCACATGATAGGTTCTATATTCATTGTGATGGAGCTTTGTTTGGACTTATGATGCCTTTTGTCAAACGTGCACCGAAAGTGACGTTTAATAAACCGATAGGGAGTGTGAGTGTATCGGGCCACAAATTTGTCGGGTGTCCAATGCCATGTGGTGTTCAGATAACAAGAATGGAACATATCAAAGTCCTCTCCAGTAACGTTGAGTACCTTGCTTCAAGGGATGCAACAATCATGGGAAGTCGTAACGGGCATGCTCCTTTGTTCCTCTGGTACACCTTAAACAGGAAAGGTTACAAAGGATTCCAGAAAGAAGTTCAGAAATGCTTGAGAAACGCGCATTACCTCAAAGACCGACTTCGTGAAGCTGGGATTAGCGCCATGCTTAATGAGCTTAGCAGCACTGTGGTCTTTGAACGGCCTAAAGATGAAGAGTTTGTTAGAAGGTGGCAGCTTGCTTGCCAAGGTGATATAGCTCATGTGGTGGTTATGCCAAGTGTTACAATCGAGAAGCTTGATAATTTCCTGAAAGACCTTGTCAAACACAGATTGATCTGGTACGAGGATGGATCTCAGCCTCCTTGCCTTGCATCGGAGGTAGGAACCAACAACTGCATCTGTCCAGCTCACAAGTGA

Protein Analysis

482

Amino Acids

54.08

Weight (kDa)

5.8

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 193 - 406 1.7e-24 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43710
fragaria_vesca FvH4_2g14160
malus_domestica MD05G1021600.v1.1 MD10G1023600.v1.1
prunus_persica Prupe.1G560500_v2.0.a1 Prupe.8G026000_v2.0.a1
pyrus_communis pycom05g01270 pycom10g01550 pycom10g01560
rosa_chinensis RchiOBHm_Chr6g0276311
rosa_laevigata RLG00000013405
rosa_multiflora Rmu_sc0003665.1_g000004
rosa_roxburghii Rroxscaffold_7G00192400
rosa_rugosa Rorug06G0099800
rosa_samantha Rh6AG211100 Rh6BG215400 Rh6CG217800 Rh6DG207800
rosa_wichuraiana Rw6G018420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AccII CGCG 2 cut(s) 592, 1151
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 1 cut(s) 90
AclI AACGTT 1 cut(s) 1014
AclWI GGATC 4 cut(s) 16, 77, 392, 1390
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 2 cut(s) 236, 941
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 5 cut(s) 426, 523, 1022, 1088, 1373
AfiI CCNNNNNNNGG 2 cut(s) 1098, 1405
AgsI TTSAA 7 cut(s) 36, 302, 410, 443, 803, 1032, 1229
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
Alw21I GWGCWC 1 cut(s) 889
Alw44I GTGCAC 1 cut(s) 885
AlwI GGATC 4 cut(s) 16, 77, 392, 1390
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 3 cut(s) 39, 934, 1232
ApaLI GTGCAC 1 cut(s) 885
ApeKI GCWGC 3 cut(s) 620, 1212, 1264
ApoI RAATTY 2 cut(s) 236, 941
AseI ATTAAT 1 cut(s) 384
AspLEI GCGC 3 cut(s) 358, 1153, 1193
AspS9I GGNCC 1 cut(s) 934
AsuHPI GGTGA 3 cut(s) 91, 232, 1292
BaeGI GKGCMC 1 cut(s) 889
BalI TGGCCA 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 889
BbvI GCAGC 3 cut(s) 607, 1224, 1276
BccI CCATC 4 cut(s) 166, 566, 839, 1373
BceAI ACGGC 2 cut(s) 77, 1247
BcgI CGANNNNNNTGC 4 cut(s) 335, 369, 532, 566
BclI TGATCA 1 cut(s) 22
BfaI CTAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 1194
BisI GCNGC 3 cut(s) 621, 1213, 1265
BlpI GCTNAGC 1 cut(s) 1208
BlsI GCNGC 3 cut(s) 622, 1214, 1266
BmgT120I GGNCC 1 cut(s) 934
BmiI GGNNCC 2 cut(s) 219, 1417
BmsI GCATC 4 cut(s) 858, 1027, 1412, 1437
BpmI CTGGAG 1 cut(s) 991
Bpu1102I GCTNAGC 1 cut(s) 1208
BpuEI CTTGAG 2 cut(s) 415, 1162
BsaJI CCNNGG 1 cut(s) 1276
BsaWI WCCGGW 5 cut(s) 118, 220, 242, 285, 568
BsaXI ACNNNNNCTCC 2 cut(s) 910, 940
Bsc4I CCNNNNNNNGG 2 cut(s) 1098, 1405
Bse118I RCCGGY 1 cut(s) 724
Bse1I ACTGG 1 cut(s) 1008
BseAI TCCGGA 1 cut(s) 568
BseDI CCNNGG 1 cut(s) 1276
BseGI GGATG 3 cut(s) 577, 1042, 1384
BseLI CCNNNNNNNGG 2 cut(s) 1098, 1405
BseMII CTCAG 1 cut(s) 1400
BseNI ACTGG 1 cut(s) 1008
BseRI GAGGAG 5 cut(s) 105, 165, 168, 171, 174
BseSI GKGCMC 1 cut(s) 889
BseXI GCAGC 3 cut(s) 607, 1224, 1276
BseYI CCCAGC 1 cut(s) 1181
Bsh1236I CGCG 2 cut(s) 592, 1151
BshFI GGCC 3 cut(s) 41, 936, 1234
BsiHKAI GWGCWC 1 cut(s) 889
BsiSI CCGG 6 cut(s) 119, 221, 243, 286, 569, 725
BslI CCNNNNNNNGG 2 cut(s) 1098, 1405
BsnI GGCC 3 cut(s) 41, 936, 1234
Bsp1286I GDGCHC 1 cut(s) 889
Bsp13I TCCGGA 1 cut(s) 568
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
Bsp1720I GCTNAGC 1 cut(s) 1208
BspACI CCGC 1 cut(s) 90
BspANI GGCC 3 cut(s) 41, 936, 1234
BspCNI CTCAG 1 cut(s) 1399
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 2 cut(s) 592, 1151
BspLI GGNNCC 2 cut(s) 219, 1417
BspPI GGATC 4 cut(s) 16, 77, 392, 1390
BsrFI RCCGGY 1 cut(s) 724
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 1 cut(s) 1008
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 1 cut(s) 1276
BssMI GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
BssT1I CCWWGG 1 cut(s) 1276
Bst4CI ACNGT 3 cut(s) 108, 757, 1219
Bst6I CTCTTC 2 cut(s) 798, 1239
BstAUI TGTACA 1 cut(s) 424
BstC8I GCNNGC 3 cut(s) 1068, 1269, 1273
BstDEI CTNAG 2 cut(s) 1208, 1386
BstENI CCTNNNNNAGG 1 cut(s) 1096
BstF5I GGATG 3 cut(s) 577, 1042, 1384
BstFNI CGCG 2 cut(s) 592, 1151
BstH2I RGCGCY 1 cut(s) 1194
BstHHI GCGC 3 cut(s) 358, 1153, 1193
BstKTI GATC 7 cut(s) 11, 25, 85, 400, 778, 1368, 1385
BstMBI GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
BstMWI GCNNNNNNNGC 3 cut(s) 263, 598, 1204
BstNSI RCATGY 1 cut(s) 1070
BstSLI GKGCMC 1 cut(s) 889
BstUI CGCG 2 cut(s) 592, 1151
BstV1I GCAGC 3 cut(s) 607, 1224, 1276
BstX2I RGATCY 3 cut(s) 8, 397, 1382
BstYI RGATCY 3 cut(s) 8, 397, 1382
BsuRI GGCC 3 cut(s) 41, 936, 1234
BtsCI GGATG 3 cut(s) 577, 1042, 1384
BtsIMutI CAGTG 1 cut(s) 1215
Cac8I GCNNGC 3 cut(s) 1068, 1269, 1273
CfoI GCGC 3 cut(s) 358, 1153, 1193
Cfr10I RCCGGY 1 cut(s) 724
Cfr13I GGNCC 1 cut(s) 934
CseI GACGC 1 cut(s) 576
Csp6I GTAC 5 cut(s) 425, 522, 1021, 1087, 1372
CviAII CATG 7 cut(s) 539, 821, 963, 1048, 1067, 1195, 1292
CviQI GTAC 5 cut(s) 425, 522, 1021, 1087, 1372
DdeI CTNAG 2 cut(s) 1208, 1386
DpnI GATC 7 cut(s) 10, 24, 84, 399, 777, 1367, 1384
DpnII GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
DraI TTTAAA 1 cut(s) 616
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 2 cut(s) 798, 1239
EarI CTCTTC 2 cut(s) 798, 1239
Eco130I CCWWGG 1 cut(s) 1276
Eco57I CTGAAG 1 cut(s) 546
EcoNI CCTNNNNNAGG 1 cut(s) 1096
EcoRI GAATTC 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 1276
ErhI CCWWGG 1 cut(s) 1276
FaeI CATG 7 cut(s) 542, 824, 966, 1051, 1070, 1198, 1295
FalI AAGNNNNNCTT 4 cut(s) 519, 551, 1155, 1187
FatI CATG 7 cut(s) 538, 820, 962, 1047, 1066, 1194, 1291
FbaI TGATCA 1 cut(s) 22
Fnu4HI GCNGC 3 cut(s) 621, 1213, 1265
FokI GGATG 3 cut(s) 584, 1049, 1391
Fsp4HI GCNGC 3 cut(s) 621, 1213, 1265
FspBI CTAG 1 cut(s) 267
GlaI GCGC 3 cut(s) 357, 1152, 1192
GluI GCNGC 3 cut(s) 621, 1213, 1265
GsaI CCCAGC 1 cut(s) 1185
GsuI CTGGAG 1 cut(s) 991
HaeII RGCGCY 1 cut(s) 1194
HaeIII GGCC 3 cut(s) 41, 936, 1234
HapII CCGG 6 cut(s) 119, 221, 243, 286, 569, 725
HgaI GACGC 1 cut(s) 576
HhaI GCGC 3 cut(s) 358, 1153, 1193
Hin1II CATG 7 cut(s) 542, 824, 966, 1051, 1070, 1198, 1295
Hin6I GCGC 3 cut(s) 356, 1151, 1191
HinP1I GCGC 3 cut(s) 356, 1151, 1191
HincII GTYRAC 2 cut(s) 229, 742
HindII GTYRAC 2 cut(s) 229, 742
HindIII AAGCTT 2 cut(s) 255, 1325
HinfI GANTC 5 cut(s) 17, 132, 596, 730, 1116
HpaI GTTAAC 2 cut(s) 229, 742
HpaII CCGG 6 cut(s) 119, 221, 243, 286, 569, 725
HphI GGTGA 3 cut(s) 91, 232, 1292
Hpy166II GTNNAC 6 cut(s) 229, 425, 427, 742, 887, 1089
Hpy188I TCNGA 6 cut(s) 22, 73, 137, 975, 1134, 1408
Hpy188III TCNNGA 9 cut(s) 432, 553, 569, 779, 800, 1120, 1175, 1321, 1339
Hpy8I GTNNAC 6 cut(s) 229, 425, 427, 742, 887, 1089
Hpy99I CGWCG 2 cut(s) 592, 757
HpyAV CCTTC 4 cut(s) 521, 545, 643, 1251
HpyCH4III ACNGT 3 cut(s) 108, 757, 1219
HpyCH4IV ACGT 4 cut(s) 200, 883, 899, 1014
HpyCH4V TGCA 4 cut(s) 887, 1040, 1403, 1428
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 598, 1204
HpyF3I CTNAG 2 cut(s) 1208, 1386
HpySE526I ACGT 4 cut(s) 200, 883, 899, 1014
Hsp92II CATG 7 cut(s) 542, 824, 966, 1051, 1070, 1198, 1295
HspAI GCGC 3 cut(s) 356, 1151, 1191
Kpn2I TCCGGA 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 22
KspAI GTTAAC 2 cut(s) 229, 742
Kzo9I GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
LmnI GCTCC 4 cut(s) 137, 764, 848, 1075
Lsp1109I GCAGC 3 cut(s) 607, 1224, 1276
LweI GCATC 4 cut(s) 858, 1027, 1412, 1437
MaeI CTAG 1 cut(s) 267
MaeII ACGT 4 cut(s) 200, 883, 899, 1014
MalI GATC 7 cut(s) 10, 24, 84, 399, 777, 1367, 1384
MboI GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
MboII GAAGA 3 cut(s) 709, 815, 1256
MfeI CAATTG 1 cut(s) 514
MflI RGATCY 3 cut(s) 8, 397, 1382
MhlI GDGCHC 1 cut(s) 889
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 4 cut(s) 236, 514, 941, 1333
MluNI TGGCCA 1 cut(s) 41
MmeI TCCRAC 2 cut(s) 110, 435
Mox20I TGGCCA 1 cut(s) 41
MroI TCCGGA 1 cut(s) 568
MscI TGGCCA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 843
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 6 cut(s) 119, 221, 243, 286, 569, 725
MunI CAATTG 1 cut(s) 514
MvnI CGCG 2 cut(s) 592, 1151
MwoI GCNNNNNNNGC 3 cut(s) 263, 598, 1204
NdeII GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
NlaIII CATG 7 cut(s) 542, 824, 966, 1051, 1070, 1198, 1295
NlaIV GGNNCC 2 cut(s) 219, 1417
NmuCI GTSAC 3 cut(s) 97, 201, 895
NspI RCATGY 1 cut(s) 1070
PaeI GCATGC 1 cut(s) 1070
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 631
PfeI GAWTC 5 cut(s) 17, 132, 596, 730, 1116
PflFI GACNNNGTC 1 cut(s) 1349
PkrI GCNGC 3 cut(s) 622, 1214, 1266
PshBI ATTAAT 1 cut(s) 384
PsiI TTATAA 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 1014
PspFI CCCAGC 1 cut(s) 1181
PspN4I GGNNCC 2 cut(s) 219, 1417
PspPI GGNCC 1 cut(s) 934
PsuI RGATCY 3 cut(s) 8, 397, 1382
PsyI GACNNNGTC 1 cut(s) 1349
RsaI GTAC 5 cut(s) 426, 523, 1022, 1088, 1373
RsaNI GTAC 5 cut(s) 425, 522, 1021, 1087, 1372
RseI CAYNNNNRTG 1 cut(s) 843
SatI GCNGC 3 cut(s) 621, 1213, 1265
Sau3AI GATC 7 cut(s) 8, 22, 82, 397, 775, 1365, 1382
Sau96I GGNCC 1 cut(s) 934
SduI GDGCHC 1 cut(s) 889
SfaNI GCATC 4 cut(s) 858, 1027, 1412, 1437
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 2 cut(s) 430, 1141
SmoI CTYRAG 2 cut(s) 430, 1141
SphI GCATGC 1 cut(s) 1070
Sse9I AATT 4 cut(s) 236, 514, 941, 1333
SsiI CCGC 1 cut(s) 90
SspMI CTAG 1 cut(s) 267
StyI CCWWGG 1 cut(s) 1276
TaaI ACNGT 3 cut(s) 108, 757, 1219
TaiI ACGT 4 cut(s) 203, 886, 902, 1017
TaqI TCGA 8 cut(s) 51, 60, 81, 345, 380, 625, 634, 1320
TaqII GACCGA 1 cut(s) 1182
TasI AATT 4 cut(s) 236, 514, 941, 1333
TatI WGTACW 1 cut(s) 424
TfiI GAWTC 5 cut(s) 17, 132, 596, 730, 1116
TscAI CASTG 1 cut(s) 1222
TseFI GTSAC 3 cut(s) 97, 201, 895
TseI GCWGC 3 cut(s) 620, 1212, 1264
Tsp45I GTSAC 3 cut(s) 97, 201, 895
TspDTI ATGAA 4 cut(s) 249, 527, 827, 1257
TspGWI ACGGA 2 cut(s) 230, 390
TspRI CASTG 1 cut(s) 1222
Tth111I GACNNNGTC 1 cut(s) 1349
VneI GTGCAC 1 cut(s) 885
VspI ATTAAT 1 cut(s) 384
XagI CCTNNNNNAGG 1 cut(s) 1096
XapI RAATTY 2 cut(s) 236, 941
XceI RCATGY 1 cut(s) 1070
XspI CTAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.