pycom10g01550

Histidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012320
Physical Location & Seq
Forward (+)
1634308 .. 1636108
1801 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g01550.1

Sequence Viewer

Length: 996 bp
ATGGATCATGAGAACGGAGCTGTGGCGGTGGAGATGCTATCCGATGATTTCGATCCCACGGCTGTGGTTGCGGAGCCTTTGCCTCCGGTTGTAACCAGCACCGACGACTGCGATCTCCTCGGAAAATTGGCCGAAGACCACAAAGGTAGTCGGGAGAAACAGATGGTTCTCGGCCGGAACGTGCACACCACGTGCCTCGCCGTGACGGAGCCCGAATCCAACGACGATTTCACTGGCGATAAGGAGGCGTACATGGCCAGTGTCCTCGCGCGCTACCGCAAAACCCTTATCGAGAGGACCAAGCACCATCTGGGTTATCCGTACAATTTGGACTTCGACTATGGTGCTCTGACGCAGCTTCAGCATTTCTCCATCAACAATTTGGGCGATCCGTTTATCGAGAGCAACTACGGTGTCCATTCAAGGCAGTTCGAGGTCGGTGTTCTCGATTGGTTTGCTCGGTTGTGGGAGATTGAGAAGAATGAGTATTGGGGATACATCACCAATTGCGGCACGGAAGGCAATCTTCACGGAGTCTTAGTTGGGAGAGAAGTATTTCCAGATGGGATTCTGTATGCTTCTCGAGAATCGCATTATTCGATATTTAAAGCTGCCAGAATGTATAGAATGGAATGCGAAAAGATTGATTGTCTGATCTCTCGTGAGATTGATTGTGCGGATTTTAAAACTAAACTTCTTGCTCACCAGGATAAACCCGCCATCATCAACGTTAATATTGTGCAGGAACAACTGTCAAAGGAGCTGTTGACGACCTGGATCTCGTCATACAGACCCTTGAAGAATGTGGATACAAGATATCGATCGTTTCTACATTCACTGCGACGGAGCATTATTTGGACTTATGATGCCTTTTGTCAAACGTGCACCAAAAGTTTCGTTCAAGAAGCCCATTGGGAGTGTCAGCGTTTCTGGTCACAAGTTTGTTGGATGTCCAATGCCTTGTGGTGTTCAGATAACAAGGCTGGAGCACATTAA

Protein Analysis

332

Amino Acids

38.12

Weight (kDa)

5.46

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43710
fragaria_vesca FvH4_2g14160
malus_domestica MD05G1021600.v1.1 MD10G1023600.v1.1
prunus_persica Prupe.1G560500_v2.0.a1 Prupe.8G026000_v2.0.a1
pyrus_communis pycom05g01270 pycom10g01550 pycom10g01560
rosa_chinensis RchiOBHm_Chr6g0276311
rosa_laevigata RLG00000013405
rosa_multiflora Rmu_sc0003665.1_g000004
rosa_roxburghii Rroxscaffold_7G00192400
rosa_rugosa Rorug06G0099800
rosa_samantha Rh6AG211100 Rh6BG215400 Rh6CG217800 Rh6DG207800
rosa_wichuraiana Rw6G018420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 269, 271
AciI CCGC 6 cut(s) 26, 71, 277, 510, 677, 717
AclI AACGTT 1 cut(s) 729
AclWI GGATC 4 cut(s) 12, 47, 383, 785
AcoI YGGCCR 3 cut(s) 129, 172, 255
AcuI CTGAAG 1 cut(s) 344
AcvI CACGTG 1 cut(s) 192
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 2 cut(s) 251, 323
AgsI TTSAA 3 cut(s) 423, 799, 902
AjnI CCWGG 2 cut(s) 705, 773
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AleI CACNNNNGTG 1 cut(s) 62
AluBI AGCT 4 cut(s) 20, 358, 611, 763
AluI AGCT 4 cut(s) 20, 358, 611, 763
Alw21I GWGCWC 4 cut(s) 186, 349, 887, 991
Alw44I GTGCAC 2 cut(s) 182, 883
AlwI GGATC 4 cut(s) 12, 47, 383, 785
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 3 cut(s) 129, 172, 255
ApaLI GTGCAC 2 cut(s) 182, 883
ApeKI GCWGC 2 cut(s) 355, 611
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 2 cut(s) 271, 273
AspS9I GGNCC 1 cut(s) 297
AsuHPI GGTGA 2 cut(s) 493, 695
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 1 cut(s) 297
BaeGI GKGCMC 2 cut(s) 186, 887
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 1 cut(s) 660
BbrPI CACGTG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 4 cut(s) 186, 349, 887, 991
BbvI GCAGC 2 cut(s) 367, 598
BccI CCATC 5 cut(s) 157, 315, 380, 557, 728
BceAI ACGGC 2 cut(s) 75, 185
BcgI CGANNNNNNTGC 4 cut(s) 326, 360, 437, 471
BciT130I CCWGG 2 cut(s) 707, 775
BciVI GTATCC 2 cut(s) 488, 802
BfuI GTATCC 2 cut(s) 488, 802
BisI GCNGC 3 cut(s) 356, 511, 612
BlsI GCNGC 3 cut(s) 357, 512, 613
Bme1390I CCNGG 2 cut(s) 707, 775
Bme18I GGWCC 1 cut(s) 297
BmeT110I CYCGRG 1 cut(s) 582
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 75, 210
BmrFI CCNGG 2 cut(s) 707, 775
BmsI GCATC 2 cut(s) 24, 856
BpiI GAAGAC 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 820
BsaAI YACGTR 1 cut(s) 192
BsaBI GATNNNNATC 2 cut(s) 51, 820
BsaJI CCNNGG 2 cut(s) 57, 118
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bse1I ACTGG 2 cut(s) 238, 258
Bse8I GATNNNNATC 2 cut(s) 51, 820
BseBI CCWGG 2 cut(s) 707, 775
BseCI ATCGAT 1 cut(s) 820
BseDI CCNNGG 2 cut(s) 57, 118
BseGI GGATG 1 cut(s) 954
BseJI GATNNNNATC 2 cut(s) 51, 820
BseNI ACTGG 2 cut(s) 238, 258
BsePI GCGCGC 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 2 cut(s) 186, 887
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 2 cut(s) 367, 598
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 2 cut(s) 269, 271
Bsh1285I CGRYCG 2 cut(s) 175, 824
BshFI GGCC 3 cut(s) 131, 174, 257
BshVI ATCGAT 1 cut(s) 820
BsiEI CGRYCG 2 cut(s) 175, 824
BsiHKAI GWGCWC 4 cut(s) 186, 349, 887, 991
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 2 cut(s) 86, 175
BsmI GAATGC 1 cut(s) 638
BsnI GGCC 3 cut(s) 131, 174, 257
BsoBI CYCGRG 1 cut(s) 582
Bsp1286I GDGCHC 5 cut(s) 186, 213, 349, 887, 991
Bsp143I GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
BspACI CCGC 6 cut(s) 26, 71, 277, 510, 677, 717
BspANI GGCC 3 cut(s) 131, 174, 257
BspDI ATCGAT 1 cut(s) 820
BspFNI CGCG 2 cut(s) 269, 271
BspHI TCATGA 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 75, 210
BspPI GGATC 4 cut(s) 12, 47, 383, 785
BsrI ACTGG 2 cut(s) 238, 258
BssECI CCNNGG 2 cut(s) 57, 118
BssHII GCGCGC 1 cut(s) 269
BssMI GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
BssSI CACGAG 1 cut(s) 660
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 707, 775
Bst4CI ACNGT 2 cut(s) 413, 753
BstBAI YACGTR 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 538
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 954
BstFNI CGCG 2 cut(s) 269, 271
BstHHI GCGC 2 cut(s) 271, 273
BstKTI GATC 7 cut(s) 7, 55, 115, 391, 657, 780, 824
BstMBI GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
BstMCI CGRYCG 2 cut(s) 175, 824
BstMWI GCNNNNNNNGC 4 cut(s) 68, 254, 361, 519
BstNI CCWGG 2 cut(s) 707, 775
BstSCI CCNGG 2 cut(s) 705, 773
BstSLI GKGCMC 2 cut(s) 186, 887
BstUI CGCG 2 cut(s) 269, 271
BstV1I GCAGC 2 cut(s) 367, 598
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 777
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 1 cut(s) 777
BstZI CGGCCG 1 cut(s) 172
Bsu15I ATCGAT 1 cut(s) 820
BsuI GTATCC 2 cut(s) 488, 802
BsuRI GGCC 3 cut(s) 131, 174, 257
BsuTUI ATCGAT 1 cut(s) 820
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 954
BtsI GCAGTG 1 cut(s) 836
BtsIMutI CAGTG 3 cut(s) 231, 265, 836
Cac8I GCNNGC 1 cut(s) 271
CciI TCATGA 1 cut(s) 7
CfoI GCGC 2 cut(s) 271, 273
Cfr13I GGNCC 1 cut(s) 297
ClaI ATCGAT 1 cut(s) 820
CseI GACGC 1 cut(s) 361
Csp6I GTAC 2 cut(s) 250, 322
CviAII CATG 2 cut(s) 8, 253
CviQI GTAC 2 cut(s) 250, 322
DdeI CTNAG 1 cut(s) 538
DpnI GATC 7 cut(s) 6, 54, 114, 390, 656, 779, 823
DpnII GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
DraI TTTAAA 2 cut(s) 607, 685
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 3 cut(s) 129, 172, 255
EagI CGGCCG 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 818
Eco47I GGWCC 1 cut(s) 297
Eco52I CGGCCG 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 344
Eco72I CACGTG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 582
EcoRII CCWGG 2 cut(s) 705, 773
EcoRV GATATC 1 cut(s) 818
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 2 cut(s) 11, 256
FaiI YATR 7 cut(s) 9, 254, 342, 576, 624, 787, 864
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FatI CATG 2 cut(s) 7, 252
FauI CCCGC 1 cut(s) 724
Fnu4HI GCNGC 3 cut(s) 356, 511, 612
FokI GGATG 1 cut(s) 961
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 356, 511, 612
GlaI GCGC 2 cut(s) 270, 272
GluI GCNGC 3 cut(s) 356, 511, 612
HaeIII GGCC 3 cut(s) 131, 174, 257
HapII CCGG 2 cut(s) 86, 175
HgaI GACGC 1 cut(s) 361
HhaI GCGC 2 cut(s) 271, 273
Hin1II CATG 2 cut(s) 11, 256
Hin6I GCGC 2 cut(s) 269, 271
HinP1I GCGC 2 cut(s) 269, 271
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HinfI GANTC 4 cut(s) 215, 534, 568, 587
HpaII CCGG 2 cut(s) 86, 175
HphI GGTGA 2 cut(s) 493, 695
Hpy166II GTNNAC 3 cut(s) 184, 768, 885
Hpy188I TCNGA 5 cut(s) 43, 122, 351, 654, 973
Hpy8I GTNNAC 3 cut(s) 184, 768, 885
Hpy99I CGWCG 3 cut(s) 107, 227, 846
HpyAV CCTTC 1 cut(s) 512
HpyCH4III ACNGT 2 cut(s) 413, 753
HpyCH4IV ACGT 4 cut(s) 180, 191, 729, 881
HpyCH4V TGCA 3 cut(s) 184, 742, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 254, 361, 519
HpyF3I CTNAG 1 cut(s) 538
HpySE526I ACGT 4 cut(s) 180, 191, 729, 881
Hsp92II CATG 2 cut(s) 11, 256
HspAI GCGC 2 cut(s) 269, 271
Kzo9I GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
LmnI GCTCC 6 cut(s) 17, 73, 208, 760, 846, 986
Lsp1109I GCAGC 2 cut(s) 367, 598
LweI GCATC 2 cut(s) 24, 856
MaeII ACGT 4 cut(s) 180, 191, 729, 881
MaeIII GTNAC 3 cut(s) 91, 202, 933
MalI GATC 7 cut(s) 6, 54, 114, 390, 656, 779, 823
MboI GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
MboII GAAGA 4 cut(s) 146, 490, 518, 811
MfeI CAATTG 1 cut(s) 505
MflI RGATCY 1 cut(s) 777
MhlI GDGCHC 5 cut(s) 186, 213, 349, 887, 991
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 4 cut(s) 125, 325, 379, 505
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 543
MmeI TCCRAC 2 cut(s) 243, 926
MnlI CCTC 7 cut(s) 93, 128, 206, 238, 275, 288, 427
Mox20I TGGCCA 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 555
MscI TGGCCA 1 cut(s) 257
MseI TTAA 4 cut(s) 606, 684, 732, 994
MslI CAYNNNNRTG 1 cut(s) 62
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 2 cut(s) 86, 175
MspR9I CCNGG 2 cut(s) 707, 775
MunI CAATTG 1 cut(s) 505
Mva1269I GAATGC 1 cut(s) 638
MvaI CCWGG 2 cut(s) 707, 775
MvnI CGCG 2 cut(s) 269, 271
MwoI GCNNNNNNNGC 4 cut(s) 68, 254, 361, 519
NdeII GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
NlaIII CATG 2 cut(s) 11, 256
NlaIV GGNNCC 2 cut(s) 75, 210
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 202, 933
OliI CACNNNNGTG 1 cut(s) 62
PaeR7I CTCGAG 1 cut(s) 582
PagI TCATGA 1 cut(s) 7
PauI GCGCGC 1 cut(s) 269
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 444, 596
PctI GAATGC 1 cut(s) 638
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 3 cut(s) 215, 568, 587
PkrI GCNGC 3 cut(s) 357, 512, 613
Ple19I CGATCG 1 cut(s) 824
PleI GAGTC 1 cut(s) 542
PmaCI CACGTG 1 cut(s) 192
PmlI CACGTG 1 cut(s) 192
PpsI GAGTC 1 cut(s) 542
Ppu21I YACGTR 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 705, 773
PspCI CACGTG 1 cut(s) 192
PspGI CCWGG 2 cut(s) 705, 773
PspN4I GGNNCC 2 cut(s) 75, 210
PspPI GGNCC 1 cut(s) 297
PsuI RGATCY 1 cut(s) 777
PteI GCGCGC 1 cut(s) 269
PvuI CGATCG 1 cut(s) 824
RsaI GTAC 2 cut(s) 251, 323
RsaNI GTAC 2 cut(s) 250, 322
RseI CAYNNNNRTG 1 cut(s) 62
SaqAI TTAA 4 cut(s) 606, 684, 732, 994
SatI GCNGC 3 cut(s) 356, 511, 612
Sau3AI GATC 7 cut(s) 4, 52, 112, 388, 654, 777, 821
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 1 cut(s) 543
ScrFI CCNGG 2 cut(s) 707, 775
SduI GDGCHC 5 cut(s) 186, 213, 349, 887, 991
SfaNI GCATC 2 cut(s) 24, 856
Sfr274I CTCGAG 1 cut(s) 582
SinI GGWCC 1 cut(s) 297
SlaI CTCGAG 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 4 cut(s) 125, 325, 379, 505
SsiI CCGC 6 cut(s) 26, 71, 277, 510, 677, 717
SspI AATATT 1 cut(s) 736
StyD4I CCNGG 2 cut(s) 705, 773
TaaI ACNGT 2 cut(s) 413, 753
TaiI ACGT 4 cut(s) 183, 194, 732, 884
TaqI TCGA 9 cut(s) 51, 291, 336, 399, 432, 447, 583, 599, 820
TasI AATT 4 cut(s) 125, 325, 379, 505
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 3 cut(s) 215, 568, 587
Tru1I TTAA 4 cut(s) 606, 684, 732, 994
Tru9I TTAA 4 cut(s) 606, 684, 732, 994
TscAI CASTG 3 cut(s) 238, 265, 843
TseFI GTSAC 2 cut(s) 202, 933
TseI GCWGC 2 cut(s) 355, 611
Tsp45I GTSAC 2 cut(s) 202, 933
TspGWI ACGGA 7 cut(s) 30, 221, 309, 381, 530, 546, 859
TspRI CASTG 3 cut(s) 238, 265, 843
VneI GTGCAC 2 cut(s) 182, 883
VpaK11BI GGWCC 1 cut(s) 297
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 379
XhoI CTCGAG 1 cut(s) 582
XmnI GAANNNNTTC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.