Prupe.1G560500_v2.0.a1

Histidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012320
Physical Location & Seq
Reverse (-)
45759810 .. 45762198
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G560500.1

Sequence Viewer

Length: 1374 bp
ATGGTTGGAAGTGTTGAGAACGAGAAGATCCAAGCGTTGCCGGAGGATTTCGTGACGACGACGGTTGATGATGAGGTGGAGAAGAAGAGGGAGATTTCGCTCGGAAGGAACGTGCACACCACATGCCATGAAGTGAGTGAGCCCGATGACGACGATGATTCCACTGGCGACAGGGAAGCTTATATGGCTAGCGTCTTGGCTCGCTACAGAAAGTCTTTGATTGAAAGGACCAAACATCATCTTGGCTACCCCTATAACTTGGACTTCGATTATGGTGCACTGGGACAGCTTCAGCACTTCTCCATCAACAACCTTGGGGATCCGTTTATCGAGAGCAACTACGGTGTTCATTCCAGACAGTTTGAAGTGGGCGTTCTGGACTGGTTTGCGCTCCTGTGGGAATTGGAGAAGAACGAATACTGGGGCTACATTACAAATTGTGGTACAGAGGGGAACCTTCATGGTATTTTGGTTGGGAGGGAAGTGTTTCCAGATGGGATTCTATATGCTTCAAAAGACTCACATTATTCTGTGTTTAAGGCAGCAAGGATGTATAGGATGGAATGTGTAAAGGTGAACACTCTTGTCTCTGGTGAGATAGATTGTGATGACTTCAAAGCCAAACTTCTTTGTCACAAAGACAAGCCAGCCATCCTAAATGTTAACATTGGAACAACTGTTAAAGGAGCTGTTGATGATCTTGATCTGGTTATCCAGAAACTTGAAGAAGCTGGCTTCACACACGACCGGTTCTATATCCACTGTGATGGGGCTCTGTTTGGTCTCATGATGCCTTTCGTCAAACGAGCGCCAAAAGTTACTTTCAAGAAACCCATAGGAAGTGTGAGTGTTTCAGGCCACAAGTTTGTTGGATGCCCAATGCCATGTGGTGTGCAAATAACAAGACTGAAGCACATTAATTGCCTCTCAAGCAATGTGGAGTATCTTGCTTCAAGGGATGCAACAATCATGGGCAGCCGAAATGGGCATGCTCCTATTTTCCTTTGGTACACCTTGAATCGAAAAGGATACGGAGGGTTCCAGAAAGAAGTTCAGAAATGCCTTAAGAATGCACATTACTTAAAAGACCGCCTACGCGAGGCCGGGATTGGTGCAATGCTGAATGAGCTGAGTAGCACGGTTGTGTTTGAGCGGCCGCAAGAGGAGGAGTTTGTTCGCAAGTGGCAGCTTGCTTGCCAAGGAAATATCGCTCATGTGATTGTAATGCCCAACATCACCATTGAAAAGCTTGATGATTTCTTGAGTGAGCTTCTTCAGCATCGTGCCAAATGGTTTCAAGATGGAAAGCTTCAAGCTCCTTGTATTGCGTCGGATGTGGGCACAGAGAATTGTCTATGTGCTTCGCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 map00340 map01100 map01110
Pfam Domains
Protein Families
Pyridoxal_deC

Protein Analysis

458

Amino Acids

51.6

Weight (kDa)

5.99

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43710
fragaria_vesca FvH4_2g14160
malus_domestica MD05G1021600.v1.1 MD10G1023600.v1.1
prunus_persica Prupe.1G560500_v2.0.a1 Prupe.8G026000_v2.0.a1
pyrus_communis pycom05g01270 pycom10g01550 pycom10g01560
rosa_chinensis RchiOBHm_Chr6g0276311
rosa_laevigata RLG00000013405
rosa_multiflora Rmu_sc0003665.1_g000004
rosa_roxburghii Rroxscaffold_7G00192400
rosa_rugosa Rorug06G0099800
rosa_samantha Rh6AG211100 Rh6BG215400 Rh6CG217800 Rh6DG207800
rosa_wichuraiana Rw6G018420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1153
AccII CGCG 1 cut(s) 1098
AciI CCGC 3 cut(s) 1090, 1153, 1157
AclWI GGATC 3 cut(s) 22, 314, 327
AcoI YGGCCR 1 cut(s) 1154
AcuI CTGAAG 3 cut(s) 275, 929, 1259
AfaI GTAC 2 cut(s) 445, 1010
AfiI CCNNNNNNNGG 1 cut(s) 1099
AflII CTTAAG 1 cut(s) 1064
AgeI ACCGGT 1 cut(s) 747
AleI CACNNNNGTG 1 cut(s) 1142
Alw21I GWGCWC 2 cut(s) 117, 280
Alw26I GTCTC 2 cut(s) 592, 788
Alw44I GTGCAC 2 cut(s) 113, 276
AlwI GGATC 3 cut(s) 22, 314, 327
AoxI GGCC 3 cut(s) 856, 1101, 1154
ApaLI GTGCAC 2 cut(s) 113, 276
ApeKI GCWGC 3 cut(s) 542, 975, 1186
AseI ATTAAT 1 cut(s) 918
AsiGI ACCGGT 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 2 cut(s) 391, 811
AspS9I GGNCC 1 cut(s) 228
AsuC2I CCSGG 1 cut(s) 1105
AsuHPI GGTGA 3 cut(s) 586, 605, 1228
AsuNHI GCTAGC 1 cut(s) 188
AvaII GGWCC 1 cut(s) 228
BaeGI GKGCMC 3 cut(s) 117, 280, 1343
BamHI GGATCC 1 cut(s) 319
BanII GRGCYC 2 cut(s) 144, 775
BarI GAAGNNNNNNTAC 2 cut(s) 401, 433
Bbv12I GWGCWC 2 cut(s) 117, 280
BbvI GCAGC 3 cut(s) 554, 987, 1198
BccI CCATC 6 cut(s) 311, 488, 553, 659, 761, 1295
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciVI GTATCC 1 cut(s) 1022
BcnI CCSGG 1 cut(s) 1105
BcoDI GTCTC 2 cut(s) 592, 788
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 812
BfrI CTTAAG 1 cut(s) 1064
BfuI GTATCC 1 cut(s) 1022
BisI GCNGC 5 cut(s) 543, 976, 1154, 1157, 1187
BlsI GCNGC 5 cut(s) 544, 977, 1155, 1158, 1188
Bme1390I CCNGG 1 cut(s) 1105
Bme18I GGWCC 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 228
BmiI GGNNCC 3 cut(s) 321, 455, 1040
BmrFI CCNGG 1 cut(s) 1105
BmrI ACTGGG 2 cut(s) 290, 430
BmsI GCATC 4 cut(s) 780, 863, 949, 1288
BmtI GCTAGC 1 cut(s) 192
BmuI ACTGGG 2 cut(s) 290, 430
BpuEI CTTGAG 2 cut(s) 913, 1282
BpuMI CCSGG 1 cut(s) 1105
BsaBI GATNNNNATC 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 788
BsaJI CCNNGG 2 cut(s) 313, 1198
BsaWI WCCGGW 1 cut(s) 747
Bsc4I CCNNNNNNNGG 1 cut(s) 1099
Bse118I RCCGGY 1 cut(s) 747
Bse1I ACTGG 4 cut(s) 169, 285, 386, 425
Bse3DI GCAATG 2 cut(s) 940, 1122
Bse8I GATNNNNATC 1 cut(s) 702
BseDI CCNNGG 2 cut(s) 313, 1198
BseGI GGATG 6 cut(s) 555, 564, 651, 878, 964, 1339
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 1 cut(s) 1099
BseMI GCAATG 2 cut(s) 940, 1122
BseMII CTCAG 1 cut(s) 1121
BseNI ACTGG 4 cut(s) 169, 285, 386, 425
BseRI GAGGAG 2 cut(s) 1178, 1181
BseSI GKGCMC 3 cut(s) 117, 280, 1343
BseX3I CGGCCG 1 cut(s) 1154
BseXI GCAGC 3 cut(s) 554, 987, 1198
Bsh1236I CGCG 1 cut(s) 1098
Bsh1285I CGRYCG 2 cut(s) 748, 1157
BshFI GGCC 3 cut(s) 858, 1103, 1156
BshTI ACCGGT 1 cut(s) 747
BsiEI CGRYCG 2 cut(s) 748, 1157
BsiHKAI GWGCWC 2 cut(s) 117, 280
BsiSI CCGG 3 cut(s) 41, 748, 1104
BslFI GGGAC 1 cut(s) 297
BslI CCNNNNNNNGG 1 cut(s) 1099
BsmAI GTCTC 2 cut(s) 592, 788
BsmFI GGGAC 1 cut(s) 297
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 3 cut(s) 858, 1103, 1156
Bso31I GGTCTC 1 cut(s) 788
Bsp1286I GDGCHC 5 cut(s) 117, 144, 280, 775, 1343
Bsp143I GATC 4 cut(s) 27, 319, 697, 703
BspACI CCGC 3 cut(s) 1090, 1153, 1157
BspANI GGCC 3 cut(s) 858, 1103, 1156
BspCNI CTCAG 1 cut(s) 1122
BspFNI CGCG 1 cut(s) 1098
BspHI TCATGA 1 cut(s) 786
BspLI GGNNCC 3 cut(s) 321, 455, 1040
BspOI GCTAGC 1 cut(s) 192
BspPI GGATC 3 cut(s) 22, 314, 327
BspTI CTTAAG 1 cut(s) 1064
BspTNI GGTCTC 1 cut(s) 788
BsrBI CCGCTC 1 cut(s) 1153
BsrDI GCAATG 2 cut(s) 940, 1122
BsrFI RCCGGY 1 cut(s) 747
BsrI ACTGG 4 cut(s) 169, 285, 386, 425
BssAI RCCGGY 1 cut(s) 747
BssECI CCNNGG 2 cut(s) 313, 1198
BssMI GATC 4 cut(s) 27, 319, 697, 703
BssT1I CCWWGG 2 cut(s) 313, 1198
Bst4CI ACNGT 6 cut(s) 64, 344, 360, 679, 764, 1141
Bst6I CTCTTC 1 cut(s) 80
BstAFI CTTAAG 1 cut(s) 1064
BstC8I GCNNGC 7 cut(s) 190, 202, 648, 733, 990, 1191, 1195
BstDEI CTNAG 1 cut(s) 1130
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstF5I GGATG 6 cut(s) 555, 564, 651, 878, 964, 1339
BstFNI CGCG 1 cut(s) 1098
BstH2I RGCGCY 1 cut(s) 812
BstHHI GCGC 2 cut(s) 391, 811
BstKTI GATC 4 cut(s) 30, 322, 700, 706
BstMAI GTCTC 2 cut(s) 592, 788
BstMBI GATC 4 cut(s) 27, 319, 697, 703
BstMCI CGRYCG 2 cut(s) 748, 1157
BstMWI GCNNNNNNNGC 4 cut(s) 185, 930, 1126, 1276
BstNSI RCATGY 2 cut(s) 126, 992
BstSCI CCNGG 1 cut(s) 1103
BstSFI CTRYAG 1 cut(s) 205
BstSLI GKGCMC 3 cut(s) 117, 280, 1343
BstUI CGCG 1 cut(s) 1098
BstV1I GCAGC 3 cut(s) 554, 987, 1198
BstX2I RGATCY 2 cut(s) 27, 319
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 2 cut(s) 27, 319
BstZI CGGCCG 1 cut(s) 1154
BsuI GTATCC 1 cut(s) 1022
BsuRI GGCC 3 cut(s) 858, 1103, 1156
BtsCI GGATG 6 cut(s) 555, 564, 651, 878, 964, 1339
BtsIMutI CAGTG 3 cut(s) 162, 278, 760
Cac8I GCNNGC 7 cut(s) 190, 202, 648, 733, 990, 1191, 1195
CciI TCATGA 1 cut(s) 786
CciNI GCGGCCGC 1 cut(s) 1154
CfoI GCGC 2 cut(s) 391, 811
Cfr10I RCCGGY 1 cut(s) 747
Cfr13I GGNCC 1 cut(s) 228
CseI GACGC 2 cut(s) 181, 1317
Csp6I GTAC 2 cut(s) 444, 1009
CspAI ACCGGT 1 cut(s) 747
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
CviAII CATG 8 cut(s) 123, 128, 461, 787, 885, 970, 989, 1214
CviQI GTAC 2 cut(s) 444, 1009
DdeI CTNAG 1 cut(s) 1130
DpnI GATC 4 cut(s) 29, 321, 699, 705
DpnII GATC 4 cut(s) 27, 319, 697, 703
EaeI YGGCCR 1 cut(s) 1154
EagI CGGCCG 1 cut(s) 1154
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EclXI CGGCCG 1 cut(s) 1154
Eco130I CCWWGG 2 cut(s) 313, 1198
Eco24I GRGCYC 2 cut(s) 144, 775
Eco31I GGTCTC 1 cut(s) 788
Eco47I GGWCC 1 cut(s) 228
Eco52I CGGCCG 1 cut(s) 1154
Eco57I CTGAAG 3 cut(s) 275, 929, 1259
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoT14I CCWWGG 2 cut(s) 313, 1198
EcoT38I GRGCYC 2 cut(s) 144, 775
ErhI CCWWGG 2 cut(s) 313, 1198
FaeI CATG 8 cut(s) 126, 131, 464, 790, 888, 973, 992, 1217
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FaqI GGGAC 1 cut(s) 297
FatI CATG 8 cut(s) 122, 127, 460, 786, 884, 969, 988, 1213
Fnu4HI GCNGC 5 cut(s) 543, 976, 1154, 1157, 1187
FokI GGATG 6 cut(s) 562, 571, 638, 885, 971, 1346
FriOI GRGCYC 2 cut(s) 144, 775
Fsp4HI GCNGC 5 cut(s) 543, 976, 1154, 1157, 1187
FspBI CTAG 1 cut(s) 189
GlaI GCGC 2 cut(s) 390, 810
GluI GCNGC 5 cut(s) 543, 976, 1154, 1157, 1187
HaeII RGCGCY 1 cut(s) 812
HaeIII GGCC 3 cut(s) 858, 1103, 1156
HapII CCGG 3 cut(s) 41, 748, 1104
HgaI GACGC 2 cut(s) 181, 1317
HhaI GCGC 2 cut(s) 391, 811
Hin1II CATG 8 cut(s) 126, 131, 464, 790, 888, 973, 992, 1217
Hin6I GCGC 2 cut(s) 389, 809
HinP1I GCGC 2 cut(s) 389, 809
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 3 cut(s) 177, 1247, 1307
HinfI GANTC 4 cut(s) 158, 499, 518, 1018
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 3 cut(s) 41, 748, 1104
HphI GGTGA 3 cut(s) 586, 605, 1228
Hpy166II GTNNAC 5 cut(s) 115, 278, 577, 664, 1011
Hpy188I TCNGA 3 cut(s) 104, 1056, 1333
Hpy8I GTNNAC 5 cut(s) 115, 278, 577, 664, 1011
Hpy99I CGWCG 4 cut(s) 61, 64, 155, 1333
HpyAV CCTTC 2 cut(s) 99, 467
HpyCH4III ACNGT 6 cut(s) 64, 344, 360, 679, 764, 1141
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 6 cut(s) 115, 278, 895, 962, 1073, 1115
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 930, 1126, 1276
HpyF3I CTNAG 1 cut(s) 1130
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 8 cut(s) 126, 131, 464, 790, 888, 973, 992, 1217
HspAI GCGC 2 cut(s) 389, 809
KspAI GTTAAC 1 cut(s) 664
Kzo9I GATC 4 cut(s) 27, 319, 697, 703
LmnI GCTCC 4 cut(s) 396, 686, 997, 1321
Lsp1109I GCAGC 3 cut(s) 554, 987, 1198
LweI GCATC 4 cut(s) 780, 863, 949, 1288
MaeI CTAG 1 cut(s) 189
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 52, 632, 817
MalI GATC 4 cut(s) 29, 321, 699, 705
MbiI CCGCTC 1 cut(s) 1153
MboI GATC 4 cut(s) 27, 319, 697, 703
MboII GAAGA 6 cut(s) 37, 94, 97, 421, 737, 1265
MflI RGATCY 2 cut(s) 27, 319
MhlI GDGCHC 5 cut(s) 117, 144, 280, 775, 1343
MluCI AATT 4 cut(s) 401, 436, 919, 1348
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 2 cut(s) 850, 1311
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 6 cut(s) 537, 663, 681, 918, 1065, 1082
MslI CAYNNNNRTG 2 cut(s) 765, 1142
MspCI CTTAAG 1 cut(s) 1064
MspI CCGG 3 cut(s) 41, 748, 1104
MspR9I CCNGG 1 cut(s) 1105
Mva1269I GAATGC 1 cut(s) 1075
MvnI CGCG 1 cut(s) 1098
MwoI GCNNNNNNNGC 4 cut(s) 185, 930, 1126, 1276
NciI CCSGG 1 cut(s) 1105
NdeII GATC 4 cut(s) 27, 319, 697, 703
NheI GCTAGC 1 cut(s) 188
NlaIII CATG 8 cut(s) 126, 131, 464, 790, 888, 973, 992, 1217
NlaIV GGNNCC 3 cut(s) 321, 455, 1040
NmuCI GTSAC 2 cut(s) 52, 632
NotI GCGGCCGC 1 cut(s) 1154
NspI RCATGY 2 cut(s) 126, 992
OliI CACNNNNGTG 1 cut(s) 1142
PaeI GCATGC 1 cut(s) 992
PagI TCATGA 1 cut(s) 786
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 1075
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 3 cut(s) 158, 499, 1018
PinAI ACCGGT 1 cut(s) 747
PkrI GCNGC 5 cut(s) 544, 977, 1155, 1158, 1188
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PshBI ATTAAT 1 cut(s) 918
PspN4I GGNNCC 3 cut(s) 321, 455, 1040
PspPI GGNCC 1 cut(s) 228
PsuI RGATCY 2 cut(s) 27, 319
RsaI GTAC 2 cut(s) 445, 1010
RsaNI GTAC 2 cut(s) 444, 1009
RseI CAYNNNNRTG 2 cut(s) 765, 1142
SaqAI TTAA 6 cut(s) 537, 663, 681, 918, 1065, 1082
SatI GCNGC 5 cut(s) 543, 976, 1154, 1157, 1187
Sau3AI GATC 4 cut(s) 27, 319, 697, 703
Sau96I GGNCC 1 cut(s) 228
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 1 cut(s) 1105
SduI GDGCHC 5 cut(s) 117, 144, 280, 775, 1343
SfaNI GCATC 4 cut(s) 780, 863, 949, 1288
SfcI CTRYAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 228
SmiMI CAYNNNNRTG 2 cut(s) 765, 1142
SmlI CTYRAG 3 cut(s) 928, 1064, 1261
SmoI CTYRAG 3 cut(s) 928, 1064, 1261
SphI GCATGC 1 cut(s) 992
Sse9I AATT 4 cut(s) 401, 436, 919, 1348
SsiI CCGC 3 cut(s) 1090, 1153, 1157
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 1103
StyI CCWWGG 2 cut(s) 313, 1198
TaaI ACNGT 6 cut(s) 64, 344, 360, 679, 764, 1141
TaiI ACGT 1 cut(s) 114
TaqI TCGA 3 cut(s) 267, 330, 1021
TasI AATT 4 cut(s) 401, 436, 919, 1348
TauI GCSGC 2 cut(s) 1156, 1159
TfiI GAWTC 3 cut(s) 158, 499, 1018
Tru1I TTAA 6 cut(s) 537, 663, 681, 918, 1065, 1082
Tru9I TTAA 6 cut(s) 537, 663, 681, 918, 1065, 1082
TscAI CASTG 3 cut(s) 169, 285, 767
TseFI GTSAC 2 cut(s) 52, 632
TseI GCWGC 3 cut(s) 542, 975, 1186
Tsp45I GTSAC 2 cut(s) 52, 632
TspDTI ATGAA 3 cut(s) 144, 338, 449
TspGWI ACGGA 2 cut(s) 312, 1047
TspRI CASTG 3 cut(s) 169, 285, 767
Vha464I CTTAAG 1 cut(s) 1064
VneI GTGCAC 2 cut(s) 113, 276
VpaK11BI GGWCC 1 cut(s) 228
VspI ATTAAT 1 cut(s) 918
XagI CCTNNNNNAGG 1 cut(s) 1097
XceI RCATGY 2 cut(s) 126, 992
XcmI CCANNNNNNNNNTGG 1 cut(s) 866
XmnI GAANNNNTTC 1 cut(s) 486
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.