RLG00000013405

Histidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012320
Physical Location & Seq
Forward (+)
31115553 .. 31123541
7989 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013405

Sequence Viewer

Length: 2079 bp
ATGGAGATTGACAATGGCGTTGTTGCCATGGTCGACATTCTCTCCGAGGATTTCGACCCAACAGCCGTCGTCGCCGAGCCCCTCCCTCCCGTCGTCACCGGCGACTCCAACGGCGATCTGATCGGAAAGGTCGCCGAGGATCTCAAGGGCCGGAAGAGAGAGATCGTCCTCGGCCGGAACGTCCACACCACGTGCCTCGAAGTCACCGAGCCCGACGCCAACGACGAGTTCACCGGCGATAAGGACGCCTACATGGCCGGCGTCCTCGCTCGCTACCGCAAGACTCTGATTGAGAGGACCAAGCACCATCTAGGGTATCCTTATAATCTGGACTTTGACTACGGAGCTCTGAGTCAGCTGCAGCATTTCTCCATCAACAATCTCGGTGATCCGTTTATTGAGAGCAACTATGGAGTCCATTCGAGACAGTTTGAGGTCGGTGTTCTTGATTGGTTTGCTCGGTTGTGGGAGATTGAGAAGAATGAGTACTGGGGTTACATCACCAATTGCGGTACCGAAGGAAATCTTCATGGAGTTTTAGTAGGGAGAGAAGTGCTTCCGGATGGGATTCTGTATGCTTCGAGGGATACGCATTATTCGATTTTTAAAGCGGCAAGAATGTATAGAATGGAATGCATAAAGGTTGACACTCTGATGTCTGGTGAGATTGATTGCGCCGATTTTAGAGCTAAGCTTCTTGCTAACAAGGACAAACCAGCTATCATTAACGTTAACATAGGAACAACTGTTAAAGGAGCTGTTGATGATCTGGATCTTGTTATAAAAACCCTTGAAGAATGTGGATTCAATCGTGATCGGTTTTACATTCACTGCGATGGAGCCCTATTTGGACTCATGATGCCCTTTGTCAAACTTGCACCAAAAGTTTCTTTCAAGAAGCCCATTGGAAGTGTGAGCGTTTCTGGTCACAAGTTTGTAGGATGTCCGATGCCTTGTGGTGTTCAGATAACAAGGCTGGAGCACATTAATGTCCTTTCAAATAATGTAGAATACCTTGCTTCAAGGGATGCGACAATCATGGGAAGCAGGAATGGTCATGCTCCTATTTTCCTATGGTACACCCTCAACAGAAAAGGTTACAAAGGGTTCCAAAAAGAAGTTCAGAAGTGTCTTCGAAATGCTCACTACTTGAAGGACCGTCTTCGTGATGCTGGGATCAGTGCCATGCTGAATGAGCTAAGTAGCACAGTTGTATTTGAGCGGCCTCAAGATGAGGAGTTTGTTCGTAGGTGGCAGTTGGCTTGCCAAGGAAACATTGCACATGTTGTGGTGATGCCAAGTGTGAACATTGATAAATTGGATGATTTCTTAAACGAACTAGTTGAAAAACGCTCAACTTGGTACAAGGATGACAAAGTTCAGCCTCCTTGTATTGCAGCTGATTCTCGAGGAATTAGTATTTTCCAGGATGGCCAAGGATTGCAAAGAAACACAACTCCTTTTGAGCTCTTTGGTGCAATCGAAGAATCGAGGTTTGCCGTTATTGTTCTCTCGCAAAATTATGCTTCTTCGCCCCGGCGCTTGAATGAACTTTCAAAGATCCTAGAGTGCATGAAAGATCGGAACAGGATTCTGCCGGTATTTCACGATGTTAATATCTTGGATGTCCAAAAACAAAAAGGTACTTTTGAGAAAGCATTTGACAAGCATGAAGAAAGGTTTCAGGATGACTTGGAAAAGGTTCAAGCCTGGAGAGATGCTTTAACCCAAGTGTGCAATTTTGCTGGATGGACTACCAATGATAGGAATGAAGTACAGGTTATAGAAGAAATTACTGAAGCACTGTGGAACAAACTACATTCTAGACTCAGTCCAACGGAAAAACTGCACCGGCCGACATCCACACACAACTTGGTCGCATTCACCAGCACTAGTGTTAATGAGGACAACCATGACCATAACCATCCAATAAACATTACAGATGTGACGTCAACCGCTAGACTCCAAATATTCAAGGAACACGAAGTAATGAACGAACTCTATGATTTCGAGTTTGGCAAGATTAATTCCAGGCACATATTGTCTGTTACAAAATGCAACAAAGCTAACAGGCTTTAG

Protein Analysis

693

Amino Acids

78.69

Weight (kDa)

5.89

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 190 - 403 3.4e-23 Pyridoxal-dependent decarboxylase conserved domain
TIR PF01582 469 - 611 7.3e-29 TIR domain
TIR_2 PF13676 471 - 540 9e-06 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43710
fragaria_vesca FvH4_2g14160
malus_domestica MD05G1021600.v1.1 MD10G1023600.v1.1
prunus_persica Prupe.1G560500_v2.0.a1 Prupe.8G026000_v2.0.a1
pyrus_communis pycom05g01270 pycom10g01550 pycom10g01560
rosa_chinensis RchiOBHm_Chr6g0276311
rosa_laevigata RLG00000013405
rosa_multiflora Rmu_sc0003665.1_g000004
rosa_roxburghii Rroxscaffold_7G00192400
rosa_rugosa Rorug06G0099800
rosa_samantha Rh6AG211100 Rh6BG215400 Rh6CG217800 Rh6DG207800
rosa_wichuraiana Rw6G018420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 324, 782
AatII GACGTC 1 cut(s) 1952
Acc65I GGTACC 1 cut(s) 512
AccB1I GGYRCC 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 1222
AccI GTMKAC 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 5 cut(s) 277, 510, 611, 1222, 1956
AclI AACGTT 1 cut(s) 729
AclWI GGATC 5 cut(s) 147, 383, 780, 1184, 1555
AcoI YGGCCR 4 cut(s) 172, 255, 1432, 1853
AcuI CTGAAG 1 cut(s) 1818
AcvI CACGTG 1 cut(s) 192
AcyI GRCGYC 4 cut(s) 216, 246, 261, 1949
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 6 cut(s) 488, 514, 1079, 1364, 1645, 1776
AfiI CCNNNNNNNGG 1 cut(s) 1764
AflIII ACRYGT 1 cut(s) 1282
AhlI ACTAGT 2 cut(s) 1339, 1892
AjnI CCWGG 3 cut(s) 1425, 1709, 2030
AjuI GAANNNNNNNTTGG 4 cut(s) 212, 244, 1104, 1136
Alw21I GWGCWC 3 cut(s) 349, 984, 1470
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 5 cut(s) 147, 383, 780, 1184, 1555
Ama87I CYCGRG 1 cut(s) 1407
Aor13HI TCCGGA 1 cut(s) 559
AoxI GGCC 6 cut(s) 148, 172, 255, 1223, 1432, 1853
ApeKI GCWGC 3 cut(s) 358, 361, 1397
AseI ATTAAT 2 cut(s) 987, 2025
Asp700I GAANNNNTTC 3 cut(s) 555, 1680, 1701
Asp718I GGTACC 1 cut(s) 512
AspLEI GCGC 2 cut(s) 677, 1542
AspS9I GGNCC 3 cut(s) 148, 297, 1156
AsuC2I CCSGG 1 cut(s) 1537
AsuHPI GGTGA 8 cut(s) 88, 196, 223, 398, 493, 674, 1303, 1876
AsuII TTCGAA 1 cut(s) 1135
AvaI CYCGRG 1 cut(s) 1407
AvaII GGWCC 2 cut(s) 297, 1156
BalI TGGCCA 1 cut(s) 1434
BanI GGYRCC 1 cut(s) 512
BanII GRGCYC 5 cut(s) 81, 213, 349, 844, 1470
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
BbrPI CACGTG 1 cut(s) 192
BbsI GAAGAC 2 cut(s) 1124, 1154
Bbv12I GWGCWC 3 cut(s) 349, 984, 1470
BbvI GCAGC 3 cut(s) 345, 373, 1409
BccI CCATC 7 cut(s) 315, 380, 557, 830, 1424, 1743, 1932
BceAI ACGGC 3 cut(s) 50, 127, 1484
BciT130I CCWGG 3 cut(s) 1427, 1711, 2032
BciVI GTATCC 2 cut(s) 327, 580
BcnI CCSGG 1 cut(s) 1537
BcoDI GTCTC 1 cut(s) 418
BcuI ACTAGT 2 cut(s) 1339, 1892
BfaI CTAG 6 cut(s) 311, 1340, 1565, 1824, 1893, 1959
BfmI CTRYAG 1 cut(s) 359
BfoI RGCGCY 1 cut(s) 1543
BfuI GTATCC 2 cut(s) 327, 580
BglI GCCNNNNNGGC 1 cut(s) 254
BisI GCNGC 5 cut(s) 359, 362, 612, 1223, 1398
BlpI GCTNAGC 1 cut(s) 690
BlsI GCNGC 5 cut(s) 360, 363, 613, 1224, 1399
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 4 cut(s) 1427, 1537, 1711, 2032
Bme18I GGWCC 2 cut(s) 297, 1156
BmeT110I CYCGRG 1 cut(s) 1407
BmgT120I GGNCC 3 cut(s) 148, 297, 1156
BmiI GGNNCC 3 cut(s) 514, 841, 1109
BmrFI CCNGG 4 cut(s) 1427, 1537, 1711, 2032
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 6 cut(s) 849, 939, 1018, 1159, 1284, 1708
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 2 cut(s) 1124, 1154
BpmI CTGGAG 2 cut(s) 998, 1732
Bpu1102I GCTNAGC 1 cut(s) 690
Bpu14I TTCGAA 1 cut(s) 1135
BpuEI CTTGAG 2 cut(s) 128, 1212
BpuMI CCSGG 1 cut(s) 1537
BsaAI YACGTR 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 771
BsaHI GRCGYC 4 cut(s) 216, 246, 261, 1949
BsaJI CCNNGG 7 cut(s) 27, 45, 135, 169, 1267, 1435, 1535
BsaWI WCCGGW 1 cut(s) 559
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 525, 555
Bsc4I CCNNNNNNNGG 1 cut(s) 1764
Bse118I RCCGGY 5 cut(s) 98, 233, 257, 1597, 1851
Bse1I ACTGG 1 cut(s) 494
Bse3DI GCAATG 1 cut(s) 1275
Bse8I GATNNNNATC 1 cut(s) 771
BseAI TCCGGA 1 cut(s) 559
BseBI CCWGG 3 cut(s) 1427, 1711, 2032
BseDI CCNNGG 7 cut(s) 27, 45, 135, 169, 1267, 1435, 1535
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 1 cut(s) 1764
BseMI GCAATG 1 cut(s) 1275
BseMII CTCAG 2 cut(s) 341, 1843
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 1 cut(s) 1250
BseX3I CGGCCG 2 cut(s) 172, 1853
BseXI GCAGC 3 cut(s) 345, 373, 1409
BseYI CCCAGC 1 cut(s) 1172
BsgI GTGCAG 1 cut(s) 1832
Bsh1285I CGRYCG 2 cut(s) 175, 1856
BshFI GGCC 6 cut(s) 150, 174, 257, 1225, 1434, 1855
BshNI GGYRCC 1 cut(s) 512
BsiEI CGRYCG 2 cut(s) 175, 1856
BsiHKAI GWGCWC 3 cut(s) 349, 984, 1470
BsiHKCI CYCGRG 1 cut(s) 1407
BsiSI CCGG 9 cut(s) 99, 151, 175, 234, 258, 560, 1537, 1598, 1852
BslI CCNNNNNNNGG 1 cut(s) 1764
BsmAI GTCTC 1 cut(s) 418
BsmI GAATGC 2 cut(s) 638, 1880
BsnI GGCC 6 cut(s) 150, 174, 257, 1225, 1434, 1855
BsoBI CYCGRG 1 cut(s) 1407
Bsp119I TTCGAA 1 cut(s) 1135
Bsp1286I GDGCHC 6 cut(s) 81, 213, 349, 844, 984, 1470
Bsp13I TCCGGA 1 cut(s) 559
Bsp1720I GCTNAGC 1 cut(s) 690
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 5 cut(s) 277, 510, 611, 1222, 1956
BspANI GGCC 6 cut(s) 150, 174, 257, 1225, 1434, 1855
BspCNI CTCAG 2 cut(s) 342, 1842
BspEI TCCGGA 1 cut(s) 559
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 3 cut(s) 514, 841, 1109
BspMAI CTGCAG 1 cut(s) 363
BspPI GGATC 5 cut(s) 147, 383, 780, 1184, 1555
BspT104I TTCGAA 1 cut(s) 1135
BspT107I GGYRCC 1 cut(s) 512
BsrBI CCGCTC 1 cut(s) 1222
BsrDI GCAATG 1 cut(s) 1275
BsrFI RCCGGY 5 cut(s) 98, 233, 257, 1597, 1851
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 5 cut(s) 98, 233, 257, 1597, 1851
BssECI CCNNGG 7 cut(s) 27, 45, 135, 169, 1267, 1435, 1535
BssNI GRCGYC 4 cut(s) 216, 246, 261, 1949
BssT1I CCWWGG 3 cut(s) 27, 1267, 1435
Bst2UI CCWGG 3 cut(s) 1427, 1711, 2032
Bst4CI ACNGT 5 cut(s) 429, 748, 1160, 1210, 1806
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 4 cut(s) 216, 246, 261, 1949
BstBAI YACGTR 1 cut(s) 192
BstBI TTCGAA 1 cut(s) 1135
BstC8I GCNNGC 3 cut(s) 259, 271, 1264
BstDEI CTNAG 4 cut(s) 350, 690, 1199, 1829
BstDSI CCRYGG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 1543
BstHHI GCGC 2 cut(s) 677, 1542
BstMAI GTCTC 1 cut(s) 418
BstMCI CGRYCG 2 cut(s) 175, 1856
BstMWI GCNNNNNNNGC 3 cut(s) 71, 254, 1195
BstNI CCWGG 3 cut(s) 1427, 1711, 2032
BstNSI RCATGY 1 cut(s) 1286
BstSCI CCNGG 4 cut(s) 1425, 1535, 1709, 2030
BstSFI CTRYAG 1 cut(s) 359
BstV1I GCAGC 3 cut(s) 345, 373, 1409
BstV2I GAAGAC 2 cut(s) 1124, 1154
BstX2I RGATCY 3 cut(s) 139, 772, 1560
BstYI RGATCY 3 cut(s) 139, 772, 1560
BstZI CGGCCG 2 cut(s) 172, 1853
BsuI GTATCC 2 cut(s) 327, 580
BsuRI GGCC 6 cut(s) 150, 174, 257, 1225, 1434, 1855
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 849
BtsI GCAGTG 1 cut(s) 829
BtsIMutI CAGTG 3 cut(s) 829, 1186, 1802
Cac8I GCNNGC 3 cut(s) 259, 271, 1264
CciI TCATGA 1 cut(s) 855
CfoI GCGC 2 cut(s) 677, 1542
Cfr10I RCCGGY 5 cut(s) 98, 233, 257, 1597, 1851
Cfr13I GGNCC 3 cut(s) 148, 297, 1156
CseI GACGC 3 cut(s) 224, 250, 254
Csp6I GTAC 6 cut(s) 487, 513, 1078, 1363, 1644, 1775
CviQI GTAC 6 cut(s) 487, 513, 1078, 1363, 1644, 1775
DdeI CTNAG 4 cut(s) 350, 690, 1199, 1829
DraI TTTAAA 1 cut(s) 607
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 4 cut(s) 172, 255, 1432, 1853
EagI CGGCCG 2 cut(s) 172, 1853
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 2 cut(s) 347, 1468
EclXI CGGCCG 2 cut(s) 172, 1853
Eco130I CCWWGG 3 cut(s) 27, 1267, 1435
Eco24I GRGCYC 5 cut(s) 81, 213, 349, 844, 1470
Eco47I GGWCC 2 cut(s) 297, 1156
Eco52I CGGCCG 2 cut(s) 172, 1853
Eco53kI GAGCTC 2 cut(s) 347, 1468
Eco57I CTGAAG 1 cut(s) 1818
Eco72I CACGTG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 1407
EcoICRI GAGCTC 2 cut(s) 347, 1468
EcoRII CCWGG 3 cut(s) 1425, 1709, 2030
EcoT14I CCWWGG 3 cut(s) 27, 1267, 1435
EcoT22I ATGCAT 1 cut(s) 638
EcoT38I GRGCYC 5 cut(s) 81, 213, 349, 844, 1470
ErhI CCWWGG 3 cut(s) 27, 1267, 1435
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 5 cut(s) 359, 362, 612, 1223, 1398
FriOI GRGCYC 5 cut(s) 81, 213, 349, 844, 1470
Fsp4HI GCNGC 5 cut(s) 359, 362, 612, 1223, 1398
FspBI CTAG 6 cut(s) 311, 1340, 1565, 1824, 1893, 1959
GlaI GCGC 2 cut(s) 676, 1541
GluI GCNGC 5 cut(s) 359, 362, 612, 1223, 1398
GsaI CCCAGC 1 cut(s) 1176
GsuI CTGGAG 2 cut(s) 998, 1732
HaeII RGCGCY 1 cut(s) 1543
HaeIII GGCC 6 cut(s) 150, 174, 257, 1225, 1434, 1855
HapII CCGG 9 cut(s) 99, 151, 175, 234, 258, 560, 1537, 1598, 1852
HgaI GACGC 3 cut(s) 224, 250, 254
HhaI GCGC 2 cut(s) 677, 1542
Hin1I GRCGYC 4 cut(s) 216, 246, 261, 1949
Hin6I GCGC 2 cut(s) 675, 1540
HinP1I GCGC 2 cut(s) 675, 1540
HincII GTYRAC 4 cut(s) 34, 646, 733, 1953
HindII GTYRAC 4 cut(s) 34, 646, 733, 1953
HindIII AAGCTT 1 cut(s) 692
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 9 cut(s) 99, 151, 175, 234, 258, 560, 1537, 1598, 1852
HphI GGTGA 8 cut(s) 88, 196, 223, 398, 493, 674, 1303, 1876
Hpy166II GTNNAC 8 cut(s) 34, 184, 231, 646, 733, 1080, 1306, 1953
Hpy8I GTNNAC 8 cut(s) 34, 184, 231, 646, 733, 1080, 1306, 1953
Hpy99I CGWCG 5 cut(s) 71, 74, 95, 218, 227
HpyAV CCTTC 2 cut(s) 512, 1147
HpyCH4III ACNGT 5 cut(s) 429, 748, 1160, 1210, 1806
HpyCH4IV ACGT 4 cut(s) 180, 191, 729, 1949
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 254, 1195
HpyF3I CTNAG 4 cut(s) 350, 690, 1199, 1829
HpySE526I ACGT 4 cut(s) 180, 191, 729, 1949
Hsp92I GRCGYC 4 cut(s) 216, 246, 261, 1949
HspAI GCGC 2 cut(s) 675, 1540
Kpn2I TCCGGA 1 cut(s) 559
KpnI GGTACC 1 cut(s) 516
KroI GCCGGC 1 cut(s) 257
KroNI GCCGGC 1 cut(s) 259
KspAI GTTAAC 1 cut(s) 733
LmnI GCTCC 5 cut(s) 344, 755, 839, 979, 1066
Lsp1109I GCAGC 3 cut(s) 345, 373, 1409
LweI GCATC 6 cut(s) 849, 939, 1018, 1159, 1284, 1708
MaeI CTAG 6 cut(s) 311, 1340, 1565, 1824, 1893, 1959
MaeII ACGT 4 cut(s) 180, 191, 729, 1949
MaeIII GTNAC 7 cut(s) 94, 202, 494, 926, 1097, 1945, 2047
MbiI CCGCTC 1 cut(s) 1222
MfeI CAATTG 1 cut(s) 505
MflI RGATCY 3 cut(s) 139, 772, 1560
MhlI GDGCHC 6 cut(s) 81, 213, 349, 844, 984, 1470
MlsI TGGCCA 1 cut(s) 1434
MluCI AATT 7 cut(s) 505, 1316, 1413, 1519, 1738, 1791, 2026
MluNI TGGCCA 1 cut(s) 1434
MlyI GAGTC 7 cut(s) 98, 277, 361, 423, 846, 1821, 1956
MmeI TCCRAC 2 cut(s) 132, 1859
Mox20I TGGCCA 1 cut(s) 1434
Mph1103I ATGCAT 1 cut(s) 638
MroI TCCGGA 1 cut(s) 559
MroNI GCCGGC 1 cut(s) 257
MroXI GAANNNNTTC 3 cut(s) 555, 1680, 1701
MscI TGGCCA 1 cut(s) 1434
MslI CAYNNNNRTG 3 cut(s) 653, 834, 987
Msp20I TGGCCA 1 cut(s) 1434
MspA1I CMGCKG 2 cut(s) 358, 1400
MspI CCGG 9 cut(s) 99, 151, 175, 234, 258, 560, 1537, 1598, 1852
MspR9I CCNGG 4 cut(s) 1427, 1537, 1711, 2032
MunI CAATTG 1 cut(s) 505
Mva1269I GAATGC 2 cut(s) 638, 1880
MvaI CCWGG 3 cut(s) 1427, 1711, 2032
MwoI GCNNNNNNNGC 3 cut(s) 71, 254, 1195
NaeI GCCGGC 1 cut(s) 259
NciI CCSGG 1 cut(s) 1537
NcoI CCATGG 1 cut(s) 27
NgoMIV GCCGGC 1 cut(s) 257
NlaIV GGNNCC 3 cut(s) 514, 841, 1109
NmeAIII GCCGAG 3 cut(s) 100, 150, 160
NmuCI GTSAC 4 cut(s) 94, 202, 926, 1945
NsiI ATGCAT 1 cut(s) 638
NspI RCATGY 1 cut(s) 1286
NspV TTCGAA 1 cut(s) 1135
PaeR7I CTCGAG 1 cut(s) 1407
PagI TCATGA 1 cut(s) 855
PciI ACATGT 1 cut(s) 1282
PcsI WCGNNNNNNNCGW 5 cut(s) 99, 177, 204, 222, 596
PctI GAATGC 2 cut(s) 638, 1880
PdiI GCCGGC 1 cut(s) 259
PdmI GAANNNNTTC 3 cut(s) 555, 1680, 1701
PfeI GAWTC 5 cut(s) 568, 804, 1403, 1487, 1591
PflFI GACNNNGTC 1 cut(s) 1830
PfoI TCCNGGA 1 cut(s) 1425
PkrI GCNGC 5 cut(s) 360, 363, 613, 1224, 1399
PleI GAGTC 7 cut(s) 98, 277, 360, 422, 846, 1821, 1956
PmaCI CACGTG 1 cut(s) 192
PmlI CACGTG 1 cut(s) 192
PpsI GAGTC 7 cut(s) 98, 277, 360, 422, 846, 1821, 1956
Ppu21I YACGTR 1 cut(s) 192
PscI ACATGT 1 cut(s) 1282
PshBI ATTAAT 2 cut(s) 987, 2025
PsiI TTATAA 2 cut(s) 324, 782
Psp124BI GAGCTC 2 cut(s) 349, 1470
Psp1406I AACGTT 1 cut(s) 729
Psp6I CCWGG 3 cut(s) 1425, 1709, 2030
PspCI CACGTG 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 1172
PspGI CCWGG 3 cut(s) 1425, 1709, 2030
PspN4I GGNNCC 3 cut(s) 514, 841, 1109
PspPI GGNCC 3 cut(s) 148, 297, 1156
PstI CTGCAG 1 cut(s) 363
PsuI RGATCY 3 cut(s) 139, 772, 1560
PsyI GACNNNGTC 1 cut(s) 1830
PvuII CAGCTG 2 cut(s) 358, 1400
RsaI GTAC 6 cut(s) 488, 514, 1079, 1364, 1645, 1776
RsaNI GTAC 6 cut(s) 487, 513, 1078, 1363, 1644, 1775
RseI CAYNNNNRTG 3 cut(s) 653, 834, 987
SacI GAGCTC 2 cut(s) 349, 1470
SalI GTCGAC 1 cut(s) 32
SatI GCNGC 5 cut(s) 359, 362, 612, 1223, 1398
Sau96I GGNCC 3 cut(s) 148, 297, 1156
ScaI AGTACT 1 cut(s) 488
SchI GAGTC 7 cut(s) 98, 277, 361, 423, 846, 1821, 1956
ScrFI CCNGG 4 cut(s) 1427, 1537, 1711, 2032
SduI GDGCHC 6 cut(s) 81, 213, 349, 844, 984, 1470
SfaNI GCATC 6 cut(s) 849, 939, 1018, 1159, 1284, 1708
SfcI CTRYAG 1 cut(s) 359
Sfr274I CTCGAG 1 cut(s) 1407
SfuI TTCGAA 1 cut(s) 1135
SgrAI CRCCGGYG 2 cut(s) 98, 233
SinI GGWCC 2 cut(s) 297, 1156
SlaI CTCGAG 1 cut(s) 1407
SmiMI CAYNNNNRTG 3 cut(s) 653, 834, 987
SmlI CTYRAG 3 cut(s) 143, 1227, 1407
SmoI CTYRAG 3 cut(s) 143, 1227, 1407
SpeI ACTAGT 2 cut(s) 1339, 1892
Sse9I AATT 7 cut(s) 505, 1316, 1413, 1519, 1738, 1791, 2026
SsiI CCGC 5 cut(s) 277, 510, 611, 1222, 1956
SspI AATATT 1 cut(s) 1971
SspMI CTAG 6 cut(s) 311, 1340, 1565, 1824, 1893, 1959
SstI GAGCTC 2 cut(s) 349, 1470
StyD4I CCNGG 4 cut(s) 1425, 1535, 1709, 2030
StyI CCWWGG 3 cut(s) 27, 1267, 1435
TaaI ACNGT 5 cut(s) 429, 748, 1160, 1210, 1806
TaiI ACGT 4 cut(s) 183, 194, 732, 1952
TasI AATT 7 cut(s) 505, 1316, 1413, 1519, 1738, 1791, 2026
TatI WGTACW 2 cut(s) 486, 1774
TauI GCSGC 2 cut(s) 614, 1225
TfiI GAWTC 5 cut(s) 568, 804, 1403, 1487, 1591
TscAI CASTG 3 cut(s) 836, 1186, 1809
TseFI GTSAC 4 cut(s) 94, 202, 926, 1945
TseI GCWGC 3 cut(s) 358, 361, 1397
Tsp45I GTSAC 4 cut(s) 94, 202, 926, 1945
TspDTI ATGAA 6 cut(s) 518, 1563, 1589, 1686, 1785, 2006
TspGWI ACGGA 3 cut(s) 357, 381, 1853
TspRI CASTG 3 cut(s) 836, 1186, 1809
Tth111I GACNNNGTC 1 cut(s) 1830
VpaK11BI GGWCC 2 cut(s) 297, 1156
VspI ATTAAT 2 cut(s) 987, 2025
XbaI TCTAGA 1 cut(s) 1823
XceI RCATGY 1 cut(s) 1286
XcmI CCANNNNNNNNNTGG 1 cut(s) 1870
XhoI CTCGAG 1 cut(s) 1407
XmiI GTMKAC 1 cut(s) 33
XmnI GAANNNNTTC 3 cut(s) 555, 1680, 1701
XspI CTAG 6 cut(s) 311, 1340, 1565, 1824, 1893, 1959
ZraI GACGTC 1 cut(s) 1950
ZrmI AGTACT 1 cut(s) 488
Zsp2I ATGCAT 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.