pycom05g01270

Histidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012320
Physical Location & Seq
Forward (+)
1634578 .. 1635426
849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01270.4

Sequence Viewer

Length: 657 bp
ATGAATTGCTTCAATTGGATTGGAATTGAGCGATTATTGTTAGTACGCAGACTGATTATTTCATACGTGGCAAAATATTACAGAACAGATTCTCTAAAATTCCTCGATATTTATTACAAACCACAACCGCCAATAACGGCTATAAATACTAAACAGAAACCGACGCTCTTCCCTCCCTCCCTCTTTCCAAACTCTCCTCCCCCTCTTCTGGTGTTTCAGCTCCTCCAACTCTCGACCGTTCGATCTTTCGGCATTAGAGGTTGCATGGATCATGAAAATGGAGTTGTGGCGGTGGAGATGTTACCCAAGGATTTCGATCCGACGGCTGTGGTTACGGAGCCTCTGCCTCCGGTCGTAACCAGCACCGCCGACTGCGATCTCATTGGAAAATTGGCTGAGGACCACAAAGGAACCCGGGAGAAACAGATGGTGCTCGGCCGGAACGTGCACACCACTTGCCTCGCCGTGACGGAGCCTGAACCCAACGACGAGTTCACGGGCGATAAGGACGCCTACATGGCCAGCGTCCTCGCTCGCTACCGCAAAACCCTCGTCGAGAGGACCAAGCACCATCTCGGTAAGCATCCCGTACAATTTGGACTTCGACTATGGTGCTCTGGCGCAGCTTCAGCATTTCTCCATCAACAATTTGGGTGA

Protein Analysis

219

Amino Acids

24.58

Weight (kDa)

8.78

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43710
fragaria_vesca FvH4_2g14160
malus_domestica MD05G1021600.v1.1 MD10G1023600.v1.1
prunus_persica Prupe.1G560500_v2.0.a1 Prupe.8G026000_v2.0.a1
pyrus_communis pycom05g01270 pycom10g01550 pycom10g01560
rosa_chinensis RchiOBHm_Chr6g0276311
rosa_laevigata RLG00000013405
rosa_multiflora Rmu_sc0003665.1_g000004
rosa_roxburghii Rroxscaffold_7G00192400
rosa_rugosa Rorug06G0099800
rosa_samantha Rh6AG211100 Rh6BG215400 Rh6CG217800 Rh6DG207800
rosa_wichuraiana Rw6G018420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 128, 290, 366, 541
AclWI GGATC 2 cut(s) 276, 311
AcoI YGGCCR 2 cut(s) 436, 519
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 510
AfaI GTAC 2 cut(s) 45, 591
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 2 cut(s) 220, 626
AluI AGCT 2 cut(s) 220, 626
Alw21I GWGCWC 3 cut(s) 435, 450, 617
Alw44I GTGCAC 1 cut(s) 446
AlwI GGATC 2 cut(s) 276, 311
Ama87I CYCGRG 1 cut(s) 414
AoxI GGCC 2 cut(s) 436, 519
ApaLI GTGCAC 1 cut(s) 446
ApeKI GCWGC 1 cut(s) 623
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 2 cut(s) 537, 569
Asp700I GAANNNNTTC 2 cut(s) 8, 88
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 2 cut(s) 400, 561
AsuC2I CCSGG 2 cut(s) 415, 416
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 2 cut(s) 400, 561
BaeGI GKGCMC 1 cut(s) 450
BalI TGGCCA 1 cut(s) 521
Bbv12I GWGCWC 3 cut(s) 435, 450, 617
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 635
BccI CCATC 3 cut(s) 421, 579, 648
BceAI ACGGC 3 cut(s) 153, 339, 449
BcgI CGANNNNNNTGC 4 cut(s) 532, 566, 594, 628
BcnI CCSGG 2 cut(s) 415, 416
BglI GCCNNNNNGGC 1 cut(s) 518
BisI GCNGC 1 cut(s) 624
BlsI GCNGC 1 cut(s) 625
Bme1390I CCNGG 2 cut(s) 415, 416
Bme18I GGWCC 2 cut(s) 400, 561
BmeT110I CYCGRG 1 cut(s) 414
BmgT120I GGNCC 2 cut(s) 400, 561
BmiI GGNNCC 3 cut(s) 339, 412, 474
BmrFI CCNGG 2 cut(s) 415, 416
BmsI GCATC 1 cut(s) 592
Bpu10I CCTNAGC 1 cut(s) 396
BpuMI CCSGG 2 cut(s) 415, 416
BsaAI YACGTR 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 306, 414
BsaWI WCCGGW 1 cut(s) 349
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse8I GATNNNNATC 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 306, 414
BseGI GGATG 1 cut(s) 583
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 387
BseRI GAGGAG 2 cut(s) 186, 212
BseSI GKGCMC 1 cut(s) 450
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 635
Bsh1285I CGRYCG 3 cut(s) 237, 354, 439
BshFI GGCC 2 cut(s) 438, 521
BsiEI CGRYCG 3 cut(s) 237, 354, 439
BsiHKAI GWGCWC 3 cut(s) 435, 450, 617
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 3 cut(s) 350, 415, 439
BslI CCNNNNNNNGG 1 cut(s) 208
BsnI GGCC 2 cut(s) 438, 521
BsoBI CYCGRG 1 cut(s) 414
Bsp1286I GDGCHC 3 cut(s) 435, 450, 617
Bsp143I GATC 4 cut(s) 242, 268, 316, 376
BspACI CCGC 4 cut(s) 128, 290, 366, 541
BspANI GGCC 2 cut(s) 438, 521
BspCNI CTCAG 1 cut(s) 388
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 3 cut(s) 339, 412, 474
BspPI GGATC 2 cut(s) 276, 311
BspQI GCTCTTC 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 306, 414
BssMI GATC 4 cut(s) 242, 268, 316, 376
BssNI GRCGYC 1 cut(s) 510
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 2 cut(s) 173, 210
BstACI GRCGYC 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 523, 535
BstDEI CTNAG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 583
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 4 cut(s) 245, 271, 319, 379
BstMBI GATC 4 cut(s) 242, 268, 316, 376
BstMCI CGRYCG 3 cut(s) 237, 354, 439
BstMWI GCNNNNNNNGC 2 cut(s) 518, 629
BstSCI CCNGG 2 cut(s) 413, 414
BstSLI GKGCMC 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 635
BstZI CGGCCG 1 cut(s) 436
BsuRI GGCC 2 cut(s) 438, 521
BtsCI GGATG 1 cut(s) 583
Cac8I GCNNGC 2 cut(s) 523, 535
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 623
Cfr13I GGNCC 2 cut(s) 400, 561
Cfr9I CCCGGG 1 cut(s) 414
CseI GACGC 3 cut(s) 172, 514, 518
Csp6I GTAC 2 cut(s) 44, 590
CviAII CATG 3 cut(s) 265, 272, 517
CviJI RGCY 9 cut(s) 140, 220, 326, 340, 395, 438, 475, 521, 626
CviKI_1 RGCY 9 cut(s) 140, 220, 326, 340, 395, 438, 475, 521, 626
CviQI GTAC 2 cut(s) 44, 590
DdeI CTNAG 1 cut(s) 396
DpnI GATC 4 cut(s) 244, 270, 318, 378
DpnII GATC 4 cut(s) 242, 268, 316, 376
EaeI YGGCCR 2 cut(s) 436, 519
EagI CGGCCG 1 cut(s) 436
Eam1104I CTCTTC 2 cut(s) 173, 210
EarI CTCTTC 2 cut(s) 173, 210
EclXI CGGCCG 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 2 cut(s) 400, 561
Eco52I CGGCCG 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 3 cut(s) 268, 275, 520
FaiI YATR 6 cut(s) 64, 143, 266, 273, 518, 610
FatI CATG 3 cut(s) 264, 271, 516
Fnu4HI GCNGC 1 cut(s) 624
FokI GGATG 1 cut(s) 570
Fsp4HI GCNGC 1 cut(s) 624
GlaI GCGC 1 cut(s) 622
GluI GCNGC 1 cut(s) 624
HaeIII GGCC 2 cut(s) 438, 521
HapII CCGG 3 cut(s) 350, 415, 439
HgaI GACGC 3 cut(s) 172, 514, 518
HhaI GCGC 1 cut(s) 623
Hin1I GRCGYC 1 cut(s) 510
Hin1II CATG 3 cut(s) 268, 275, 520
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HinfI GANTC 1 cut(s) 89
HpaII CCGG 3 cut(s) 350, 415, 439
Hpy166II GTNNAC 2 cut(s) 448, 495
Hpy188I TCNGA 1 cut(s) 321
Hpy188III TCNNGA 3 cut(s) 232, 272, 556
Hpy8I GTNNAC 2 cut(s) 448, 495
Hpy99I CGWCG 4 cut(s) 166, 325, 491, 557
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 2 cut(s) 66, 444
HpyCH4V TGCA 2 cut(s) 264, 448
HpyF10VI GCNNNNNNNGC 2 cut(s) 518, 629
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 2 cut(s) 66, 444
Hsp92I GRCGYC 1 cut(s) 510
Hsp92II CATG 3 cut(s) 268, 275, 520
HspAI GCGC 1 cut(s) 621
Kzo9I GATC 4 cut(s) 242, 268, 316, 376
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 3 cut(s) 225, 337, 472
LpnPI CCDG 8 cut(s) 194, 363, 373, 428, 452, 489, 535, 603
Lsp1109I GCAGC 1 cut(s) 635
LweI GCATC 1 cut(s) 592
MaeII ACGT 2 cut(s) 66, 444
MaeIII GTNAC 4 cut(s) 300, 331, 355, 466
MalI GATC 4 cut(s) 244, 270, 318, 378
MboI GATC 4 cut(s) 242, 268, 316, 376
MboII GAAGA 2 cut(s) 160, 197
MfeI CAATTG 1 cut(s) 13
MhlI GDGCHC 3 cut(s) 435, 450, 617
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 7 cut(s) 4, 13, 24, 98, 389, 593, 647
MluNI TGGCCA 1 cut(s) 521
MmeI TCCRAC 2 cut(s) 250, 344
Mox20I TGGCCA 1 cut(s) 521
MroXI GAANNNNTTC 2 cut(s) 8, 88
MscI TGGCCA 1 cut(s) 521
MslI CAYNNNNRTG 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 521
MspI CCGG 3 cut(s) 350, 415, 439
MspR9I CCNGG 2 cut(s) 415, 416
MunI CAATTG 1 cut(s) 13
MwoI GCNNNNNNNGC 2 cut(s) 518, 629
NciI CCSGG 2 cut(s) 415, 416
NdeII GATC 4 cut(s) 242, 268, 316, 376
NlaIII CATG 3 cut(s) 268, 275, 520
NlaIV GGNNCC 3 cut(s) 339, 412, 474
NmeAIII GCCGAG 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 466
PagI TCATGA 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 173
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PdmI GAANNNNTTC 2 cut(s) 8, 88
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 625
Ppu21I YACGTR 1 cut(s) 67
PspN4I GGNNCC 3 cut(s) 339, 412, 474
PspPI GGNCC 2 cut(s) 400, 561
RsaI GTAC 2 cut(s) 45, 591
RsaNI GTAC 2 cut(s) 44, 590
RseI CAYNNNNRTG 1 cut(s) 276
SapI GCTCTTC 1 cut(s) 173
SatI GCNGC 1 cut(s) 624
Sau3AI GATC 4 cut(s) 242, 268, 316, 376
Sau96I GGNCC 2 cut(s) 400, 561
ScrFI CCNGG 2 cut(s) 415, 416
SduI GDGCHC 3 cut(s) 435, 450, 617
SetI ASST 5 cut(s) 69, 222, 262, 447, 628
SfaNI GCATC 1 cut(s) 592
SinI GGWCC 2 cut(s) 400, 561
SmaI CCCGGG 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 276
Sse9I AATT 7 cut(s) 4, 13, 24, 98, 389, 593, 647
SsiI CCGC 4 cut(s) 128, 290, 366, 541
SspI AATATT 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 413, 414
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 2 cut(s) 69, 447
TaqI TCGA 6 cut(s) 105, 233, 241, 315, 555, 604
TasI AATT 7 cut(s) 4, 13, 24, 98, 389, 593, 647
TfiI GAWTC 1 cut(s) 89
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 1 cut(s) 623
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 3 cut(s) 17, 51, 288
TspGWI ACGGA 2 cut(s) 350, 485
TspMI CCCGGG 1 cut(s) 414
VneI GTGCAC 1 cut(s) 446
VpaK11BI GGWCC 2 cut(s) 400, 561
XapI RAATTY 1 cut(s) 98
XcmI CCANNNNNNNNNTGG 1 cut(s) 647
XmaI CCCGGG 1 cut(s) 414
XmnI GAANNNNTTC 2 cut(s) 8, 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.