Rroxscaffold_7G00192400

Histidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012320
Physical Location & Seq
Forward (+)
33590334 .. 33593020
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00192400.1

Sequence Viewer

Length: 1191 bp
ATGGCCGGCGTCCTCGCTCGCTACCGCAAGACTCTGATTGAGAGGACCAAGCACCATCTGGGGTATCCTTATAATCTGGACTTCGACTATGGAGCTTTGAGTCAGCTGCAGCATTTCTCCATCAACAATCTCGGCGATCCGTTTATTGAGAGCAACTATGGAGTCCATTCGAGACAGTTTGAGGTCGGTGTTCTTGATTGGTTTGCTCGGTTGTGGGAGATTGAGAAGAATGAGTACTGGGGTTACATCACCAATTGCGGTACCGAAGGAAATCTTCATGGAGTTTTAGTAGGGAGAGAAGTGCTTCCGGATGGGATTCTGTATGCTTCGAGGGATACGCATTATTCGATATTTAAAGCGGCAAGAATGTATAGAATGGAATGCGTAAAGGTTGATACTCTGATGTCTGGTGAGATTGATTGCGCCGATTTTAGAGCTAAGCTTCTTGCTAACAAGGACAAACCAGCTATCATTAATGTTAACATAGGAACAACTGTTAAAGGAGCTGTTGACGATCTGGATCTTGTCATAAAAACCCTTGAAGAATGTGGATTCAATCGTGATCGGTTCTACATTCACTGCGATGGAGCCTTATTTGGACTCATGATGCCTTTTGTCAAACTTGCACCAAAAGTTTCTTTCAAGAAGCCCATTGGAAGTGTGAGCGTTTCTGGTCACAAGTTTGTAGGATGTCCGATGCCTTGCGGTGTTCAGATAACAAGGCTGGAGCACATTAATGTCCTTTCAAATAATGTAGAATACCTTGCTTCAAGGGATGCGACAATCATGGGAAGCAGGAATGGCCATGCTCCTATTTTCCTATGGTACACCCTCAACAGAAAAGGTTACAAAGGGTTCCAAAAAGAAGTTCAGAAGTGTCTTCGAAATGCTCACTACTTGAAGGACCGTCTTCGTGATGCTGGGATCAGTGCCATGCTGAATGAGCTAAGTAGCACAGTTGTATTTGAGCGGCCTCAAGATGAGGAGTTTGTTCGTAGGTGGCAGTTGGCTTGCCAAGGAAACATTGCACATGTTGTGGTGATGCCAAGTGTGAACATTGATAAATTGGATGATTTCTTAAACGAACTAGTTGAAAAACGCTCAACTTGGTACAAGGATGAGAAAGTTCAGCCTCCTTGTATTGCAGCTGATGTAGGGACTCAGAATTGTCTTTGCGGGAAGCACAAGTGA

Protein Analysis

396

Amino Acids

44.97

Weight (kDa)

8.17

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 106 - 319 3.2e-23 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43710
fragaria_vesca FvH4_2g14160
malus_domestica MD05G1021600.v1.1 MD10G1023600.v1.1
prunus_persica Prupe.1G560500_v2.0.a1 Prupe.8G026000_v2.0.a1
pyrus_communis pycom05g01270 pycom10g01550 pycom10g01560
rosa_chinensis RchiOBHm_Chr6g0276311
rosa_laevigata RLG00000013405
rosa_multiflora Rmu_sc0003665.1_g000004
rosa_roxburghii Rroxscaffold_7G00192400
rosa_rugosa Rorug06G0099800
rosa_samantha Rh6AG211100 Rh6BG215400 Rh6CG217800 Rh6DG207800
rosa_wichuraiana Rw6G018420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
Acc65I GGTACC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 260
AccBSI CCGCTC 1 cut(s) 970
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 6 cut(s) 25, 258, 359, 705, 970, 1176
AclWI GGATC 3 cut(s) 131, 528, 932
AcoI YGGCCR 2 cut(s) 3, 802
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 4 cut(s) 236, 262, 827, 1112
AflIII ACRYGT 1 cut(s) 1030
AgsI TTSAA 7 cut(s) 542, 556, 643, 747, 771, 901, 1094
AhlI ACTAGT 1 cut(s) 1087
AjuI GAANNNNNNNTTGG 2 cut(s) 852, 884
AluBI AGCT 8 cut(s) 95, 106, 437, 442, 467, 506, 946, 1148
AluI AGCT 8 cut(s) 95, 106, 437, 442, 467, 506, 946, 1148
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 1 cut(s) 166
AlwI GGATC 3 cut(s) 131, 528, 932
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 3 cut(s) 3, 802, 971
ApeKI GCWGC 3 cut(s) 106, 109, 1145
AseI ATTAAT 2 cut(s) 474, 735
Asp700I GAANNNNTTC 1 cut(s) 303
Asp718I GGTACC 1 cut(s) 260
AspLEI GCGC 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 45, 904
AsuHPI GGTGA 3 cut(s) 241, 422, 1051
AsuII TTCGAA 1 cut(s) 883
AvaII GGWCC 2 cut(s) 45, 904
BalI TGGCCA 1 cut(s) 804
BanI GGYRCC 1 cut(s) 260
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BbsI GAAGAC 2 cut(s) 872, 902
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 3 cut(s) 93, 121, 1157
BccI CCATC 4 cut(s) 63, 128, 305, 578
BciVI GTATCC 2 cut(s) 75, 328
BcoDI GTCTC 1 cut(s) 166
BcuI ACTAGT 1 cut(s) 1087
BfaI CTAG 1 cut(s) 1088
BfmI CTRYAG 1 cut(s) 107
BfuI GTATCC 2 cut(s) 75, 328
BisI GCNGC 5 cut(s) 107, 110, 360, 971, 1146
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 5 cut(s) 108, 111, 361, 972, 1147
BmcAI AGTACT 1 cut(s) 236
Bme18I GGWCC 2 cut(s) 45, 904
BmgT120I GGNCC 2 cut(s) 45, 904
BmiI GGNNCC 3 cut(s) 262, 589, 857
BmrI ACTGGG 1 cut(s) 247
BmsI GCATC 5 cut(s) 597, 687, 766, 907, 1032
BmuI ACTGGG 1 cut(s) 247
BpiI GAAGAC 2 cut(s) 872, 902
BpmI CTGGAG 1 cut(s) 746
Bpu1102I GCTNAGC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 883
BpuEI CTTGAG 1 cut(s) 960
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 1015
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 1023
Bse8I GATNNNNATC 1 cut(s) 519
BseAI TCCGGA 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 1015
BseGI GGATG 5 cut(s) 316, 695, 781, 1075, 1123
BseJI GATNNNNATC 1 cut(s) 519
BseMI GCAATG 1 cut(s) 1023
BseMII CTCAG 1 cut(s) 1175
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 998
BseXI GCAGC 3 cut(s) 93, 121, 1157
BseYI CCCAGC 1 cut(s) 920
BshFI GGCC 3 cut(s) 5, 804, 973
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 732
BsiSI CCGG 2 cut(s) 6, 308
BslFI GGGAC 1 cut(s) 1171
BsmAI GTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 1171
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 3 cut(s) 5, 804, 973
Bsp119I TTCGAA 1 cut(s) 883
Bsp1286I GDGCHC 1 cut(s) 732
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 5 cut(s) 136, 514, 520, 562, 924
Bsp1720I GCTNAGC 1 cut(s) 438
BspACI CCGC 6 cut(s) 25, 258, 359, 705, 970, 1176
BspANI GGCC 3 cut(s) 5, 804, 973
BspCNI CTCAG 1 cut(s) 1174
BspEI TCCGGA 1 cut(s) 307
BspHI TCATGA 1 cut(s) 603
BspLI GGNNCC 3 cut(s) 262, 589, 857
BspMAI CTGCAG 1 cut(s) 111
BspPI GGATC 3 cut(s) 131, 528, 932
BspT104I TTCGAA 1 cut(s) 883
BspT107I GGYRCC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 970
BsrDI GCAATG 1 cut(s) 1023
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 1015
BssMI GATC 5 cut(s) 136, 514, 520, 562, 924
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 1015
Bst4CI ACNGT 4 cut(s) 177, 496, 908, 958
BstACI GRCGYC 1 cut(s) 9
BstBI TTCGAA 1 cut(s) 883
BstC8I GCNNGC 3 cut(s) 7, 19, 1012
BstDEI CTNAG 3 cut(s) 438, 947, 1161
BstF5I GGATG 5 cut(s) 316, 695, 781, 1075, 1123
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 5 cut(s) 139, 517, 523, 565, 927
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 5 cut(s) 136, 514, 520, 562, 924
BstMWI GCNNNNNNNGC 2 cut(s) 801, 943
BstNSI RCATGY 1 cut(s) 1034
BstSFI CTRYAG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 93, 121, 1157
BstV2I GAAGAC 2 cut(s) 872, 902
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BsuI GTATCC 2 cut(s) 75, 328
BsuRI GGCC 3 cut(s) 5, 804, 973
BtgZI GCGATG 1 cut(s) 597
BtsCI GGATG 5 cut(s) 316, 695, 781, 1075, 1123
BtsI GCAGTG 1 cut(s) 577
BtsIMutI CAGTG 2 cut(s) 577, 934
Cac8I GCNNGC 3 cut(s) 7, 19, 1012
CciI TCATGA 1 cut(s) 603
CfoI GCGC 1 cut(s) 425
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 2 cut(s) 45, 904
Csp6I GTAC 4 cut(s) 235, 261, 826, 1111
CviAII CATG 6 cut(s) 278, 604, 787, 806, 934, 1031
CviQI GTAC 4 cut(s) 235, 261, 826, 1111
DdeI CTNAG 3 cut(s) 438, 947, 1161
DpnI GATC 5 cut(s) 138, 516, 522, 564, 926
DpnII GATC 5 cut(s) 136, 514, 520, 562, 924
DraI TTTAAA 1 cut(s) 355
EaeI YGGCCR 2 cut(s) 3, 802
Eco130I CCWWGG 1 cut(s) 1015
Eco47I GGWCC 2 cut(s) 45, 904
EcoT14I CCWWGG 1 cut(s) 1015
ErhI CCWWGG 1 cut(s) 1015
FaeI CATG 6 cut(s) 281, 607, 790, 809, 937, 1034
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 1 cut(s) 1171
FatI CATG 6 cut(s) 277, 603, 786, 805, 933, 1030
FauI CCCGC 1 cut(s) 1169
Fnu4HI GCNGC 5 cut(s) 107, 110, 360, 971, 1146
FokI GGATG 5 cut(s) 323, 702, 788, 1082, 1130
Fsp4HI GCNGC 5 cut(s) 107, 110, 360, 971, 1146
FspBI CTAG 1 cut(s) 1088
GlaI GCGC 1 cut(s) 424
GluI GCNGC 5 cut(s) 107, 110, 360, 971, 1146
GsaI CCCAGC 1 cut(s) 924
GsuI CTGGAG 1 cut(s) 746
HaeIII GGCC 3 cut(s) 5, 804, 973
HapII CCGG 2 cut(s) 6, 308
HhaI GCGC 1 cut(s) 425
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 6 cut(s) 281, 607, 790, 809, 937, 1034
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 2 cut(s) 481, 511
HindII GTYRAC 2 cut(s) 481, 511
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 7 cut(s) 31, 100, 162, 316, 552, 600, 1159
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 2 cut(s) 6, 308
HphI GGTGA 3 cut(s) 241, 422, 1051
Hpy166II GTNNAC 4 cut(s) 481, 511, 828, 1054
Hpy188I TCNGA 6 cut(s) 36, 402, 696, 714, 873, 1164
Hpy8I GTNNAC 4 cut(s) 481, 511, 828, 1054
HpyAV CCTTC 2 cut(s) 260, 895
HpyCH4III ACNGT 4 cut(s) 177, 496, 908, 958
HpyCH4V TGCA 4 cut(s) 109, 626, 1028, 1145
HpyF10VI GCNNNNNNNGC 2 cut(s) 801, 943
HpyF3I CTNAG 3 cut(s) 438, 947, 1161
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 6 cut(s) 281, 607, 790, 809, 937, 1034
HspAI GCGC 1 cut(s) 423
Kpn2I TCCGGA 1 cut(s) 307
KpnI GGTACC 1 cut(s) 264
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 5 cut(s) 136, 514, 520, 562, 924
LmnI GCTCC 5 cut(s) 92, 503, 587, 727, 814
Lsp1109I GCAGC 3 cut(s) 93, 121, 1157
LweI GCATC 5 cut(s) 597, 687, 766, 907, 1032
MaeI CTAG 1 cut(s) 1088
MaeIII GTNAC 3 cut(s) 242, 674, 845
MalI GATC 5 cut(s) 138, 516, 522, 564, 926
MbiI CCGCTC 1 cut(s) 970
MboI GATC 5 cut(s) 136, 514, 520, 562, 924
MboII GAAGA 5 cut(s) 238, 266, 554, 872, 902
MfeI CAATTG 1 cut(s) 253
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 732
MlsI TGGCCA 1 cut(s) 804
MluCI AATT 3 cut(s) 253, 1064, 1165
MluNI TGGCCA 1 cut(s) 804
MlyI GAGTC 5 cut(s) 25, 109, 171, 594, 1153
MnlI CCTC 8 cut(s) 23, 36, 175, 324, 842, 976, 984, 1143
Mox20I TGGCCA 1 cut(s) 804
MroI TCCGGA 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 303
MscI TGGCCA 1 cut(s) 804
MseI TTAA 6 cut(s) 354, 474, 480, 498, 735, 1079
MslI CAYNNNNRTG 2 cut(s) 582, 735
Msp20I TGGCCA 1 cut(s) 804
MspA1I CMGCKG 2 cut(s) 106, 1148
MspI CCGG 2 cut(s) 6, 308
MunI CAATTG 1 cut(s) 253
Mva1269I GAATGC 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 801, 943
NaeI GCCGGC 1 cut(s) 7
NdeII GATC 5 cut(s) 136, 514, 520, 562, 924
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 6 cut(s) 281, 607, 790, 809, 937, 1034
NlaIV GGNNCC 3 cut(s) 262, 589, 857
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 1 cut(s) 674
NspI RCATGY 1 cut(s) 1034
NspV TTCGAA 1 cut(s) 883
PagI TCATGA 1 cut(s) 603
PciI ACATGT 1 cut(s) 1030
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PctI GAATGC 1 cut(s) 386
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 2 cut(s) 316, 552
PkrI GCNGC 5 cut(s) 108, 111, 361, 972, 1147
PleI GAGTC 5 cut(s) 25, 108, 170, 594, 1153
PpsI GAGTC 5 cut(s) 25, 108, 170, 594, 1153
PscI ACATGT 1 cut(s) 1030
PshBI ATTAAT 2 cut(s) 474, 735
PsiI TTATAA 1 cut(s) 72
PspFI CCCAGC 1 cut(s) 920
PspN4I GGNNCC 3 cut(s) 262, 589, 857
PspPI GGNCC 2 cut(s) 45, 904
PstI CTGCAG 1 cut(s) 111
PsuI RGATCY 1 cut(s) 520
PvuII CAGCTG 2 cut(s) 106, 1148
RsaI GTAC 4 cut(s) 236, 262, 827, 1112
RsaNI GTAC 4 cut(s) 235, 261, 826, 1111
RseI CAYNNNNRTG 2 cut(s) 582, 735
SaqAI TTAA 6 cut(s) 354, 474, 480, 498, 735, 1079
SatI GCNGC 5 cut(s) 107, 110, 360, 971, 1146
Sau3AI GATC 5 cut(s) 136, 514, 520, 562, 924
Sau96I GGNCC 2 cut(s) 45, 904
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 5 cut(s) 25, 109, 171, 594, 1153
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 5 cut(s) 597, 687, 766, 907, 1032
SfcI CTRYAG 1 cut(s) 107
SfuI TTCGAA 1 cut(s) 883
SinI GGWCC 2 cut(s) 45, 904
SmiMI CAYNNNNRTG 2 cut(s) 582, 735
SmlI CTYRAG 1 cut(s) 975
SmoI CTYRAG 1 cut(s) 975
SpeI ACTAGT 1 cut(s) 1087
Sse9I AATT 3 cut(s) 253, 1064, 1165
SsiI CCGC 6 cut(s) 25, 258, 359, 705, 970, 1176
SspMI CTAG 1 cut(s) 1088
StyI CCWWGG 1 cut(s) 1015
TaaI ACNGT 4 cut(s) 177, 496, 908, 958
TaqI TCGA 5 cut(s) 84, 170, 329, 347, 883
TasI AATT 3 cut(s) 253, 1064, 1165
TatI WGTACW 1 cut(s) 234
TauI GCSGC 2 cut(s) 362, 973
TfiI GAWTC 2 cut(s) 316, 552
Tru1I TTAA 6 cut(s) 354, 474, 480, 498, 735, 1079
Tru9I TTAA 6 cut(s) 354, 474, 480, 498, 735, 1079
TscAI CASTG 2 cut(s) 584, 934
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 3 cut(s) 106, 109, 1145
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 1 cut(s) 266
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 2 cut(s) 584, 934
VpaK11BI GGWCC 2 cut(s) 45, 904
VspI ATTAAT 2 cut(s) 474, 735
XceI RCATGY 1 cut(s) 1034
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 1 cut(s) 1088
ZrmI AGTACT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.