AT1G45130

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009802
Physical Location & Seq
Forward (+)
17065111 .. 17069269
4159 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G45130.1

Sequence Viewer

Length: 2199 bp
ATGGGAACAACGATCTTGGTTCTCTCCAAGATCCTAACTTTCTTACTAACAACAATGCTGATTGGATCCAGTGTGATCCAGTGTAGTAGTGTAACCTACGATAAGAAAGCTATCGTCATCAATGGCCACCGTAGAATCCTCCTCTCTGGTTCTATTCACTACCCAAGAAGCACTCCTGAGATGTGGGAAGATCTTATAAAGAAAGCTAAAGACGGTGGCTTGGATGTTATTGATACTTATGTTTTCTGGAATGGTCATGAACCTTCTCCTGGAACTTACAATTTCGAAGGAAGATATGATTTGGTAAGATTCATTAAGACGATTCAGGAAGTGGGTCTTTATGTTCATCTCAGAATTGGTCCTTATGTTTGTGCAGAATGGAATTTTGGAGGGTTTCCTGTGTGGTTGAAGTATGTAGATGGGATTAGTTTCAGAACTGACAATGGACCCTTCAAGTCTGCAATGCAAGGATTCACAGAGAAGATTGTTCAGATGATGAAAGAACATAGATTCTTTGCGTCACAAGGTGGACCTATCATTCTTTCTCAGATTGAGAATGAGTTTGAACCAGATCTTAAAGGGCTTGGACCGGCTGGTCACTCGTATGTCAATTGGGCTGCGAAAATGGCGGTCGGTTTAAACACGGGAGTACCGTGGGTGATGTGCAAGGAAGATGATGCACCTGACCCAATTATAAACACTTGCAACGGATTCTACTGTGATTATTTTACTCCAAATAAACCGTATAAGCCAACTATGTGGACAGAGGCATGGAGTGGCTGGTTCACAGAGTTTGGTGGAACTGTTCCTAAACGACCTGTAGAGGATCTAGCATTTGGAGTAGCTCGTTTCATTCAAAAGGGCGGTTCGTATATAAACTACTACATGTACCATGGAGGAACAAACTTTGGACGCACCGCAGGAGGTCCATTTATCACCACTAGTTATGACTATGATGCTCCTATCGATGAATACGGGTTGGTCCAAGAGCCCAAGTACAGTCATCTTAAGCAGCTTCATCAGGCAATCAAGCAATGTGAAGCTGCCTTAGTTTCTTCTGATCCACATGTTACTAAACTAGGAAACTACGAGGAGGCTCATGTGTTCACTGCTGGCAAAGGAAGTTGTGTAGCTTTCTTAACGAACTATCACATGAATGCACCTGCAAAAGTAGTGTTCAATAACCGACACTATACTCTACCTGCTTGGTCCATCAGCATTCTTCCAGATTGTAGAAACGTTGTTTTCAACACTGCGACGGTTGCTGCAAAGACATCACATGTGCAAATGGTGCCATCTGGTTCCATATTGTACTCGGTTGCTAGATACGATGAAGATATTGCTACTTATGGAAACCGTGGGACAATCACAGCTCGTGGATTGTTGGAGCAGGTTAATGTTACACGAGATACAACTGATTACCTGTGGTACACAACCAGTGTGGATATTAAGGCATCAGAATCATTCTTGCGTGGAGGAAAATGGCCAACTCTTACAGTGGATTCTGCAGGGCATGCTGTTCATGTGTTTGTCAATGGACATTTTTACGGATCTGCCTTTGGAACAAGAGAAAACAGAAAGTTTTCATTTAGCTCCCAAGTCAATCTACGAGGTGGAGCTAACAAAATCGCACTACTGAGTGTAGCAGTTGGGTTGCCGAATGTTGGACCACATTTTGAGACGTGGGCCACAGGAATCGTCGGGTCTGTGGTGCTTCATGGCCTTGACGAGGGTAACAAAGACTTGAGTTGGCAGAAATGGACTTATCAGGCTGGTCTGCGAGGGGAATCAATGAACTTGGTCTCTCCTACTGAGGACTCCTCTGTTGATTGGATCAAAGGCTCATTGGCTAAGCAAAACAAACAACCTTTGACATGGTACAAGGCCTATTTTGACGCGCCTAGAGGGAACGAGCCGCTGGCTTTGGATCTAAAGAGTATGGGGAAAGGGCAAGCTTGGATAAACGGGCAAAGCATAGGGAGATACTGGATGGCATTTGCCAAAGGAGACTGCGGAAGTTGTAACTACGCTGGAACATACAGGCAGAACAAATGCCAGTCTGGTTGTGGCGAGCCGACACAAAGATGGTATCATGTTCCGCGTTCGTGGTTGAAGCCAAAAGGGAACTTGTTAGTACTTTTTGAAGAACTTGGTGGAGATATATCCAAAGTCTCTGTTGTGAAGAGATCAGTAAACTAA

Protein Analysis

732

Amino Acids

81.44

Weight (kDa)

8.48

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 38 - 342 2.7e-122 Glycosyl hydrolases family 35
GHD PF17834 365 - 421 1.2e-22 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 474 - 539 4.7e-07 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 624 - 709 2.1e-27 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 197, 695
AarI CACCTGC 1 cut(s) 1171
AasI GACNNNNNNGTC 1 cut(s) 594
Acc36I ACCTGC 3 cut(s) 1171, 1210, 1381
AccB1I GGYRCC 1 cut(s) 1291
AccII CGCG 2 cut(s) 1898, 2101
AciI CCGC 6 cut(s) 629, 864, 918, 1916, 2013, 2099
AclI AACGTT 1 cut(s) 1239
AclWI GGATC 9 cut(s) 25, 60, 70, 73, 834, 1055, 1558, 1841, 1935
AcoI YGGCCR 2 cut(s) 124, 1484
AcsI RAATTY 1 cut(s) 382
AdeI CACNNNGTG 1 cut(s) 527
AfaI GTAC 7 cut(s) 651, 890, 998, 1313, 1430, 1880, 2136
AfiI CCNNNNNNNGG 3 cut(s) 269, 1664, 1729
AflII CTTAAG 1 cut(s) 1007
AflIII ACRYGT 3 cut(s) 885, 1066, 1279
AgsI TTSAA 8 cut(s) 409, 454, 566, 857, 1180, 1249, 2113, 2144
AhlI ACTAGT 1 cut(s) 941
AjiI CACGTC 1 cut(s) 1683
AjnI CCWGG 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
Alw26I GTCTC 4 cut(s) 1673, 1807, 2001, 2176
AlwI GGATC 9 cut(s) 25, 60, 70, 73, 834, 1055, 1558, 1841, 1935
AoxI GGCC 5 cut(s) 124, 1484, 1686, 1720, 1884
ApeKI GCWGC 4 cut(s) 617, 1012, 1043, 1265
ApoI RAATTY 1 cut(s) 382
Asp700I GAANNNNTTC 1 cut(s) 1582
AspLEI GCGC 1 cut(s) 1900
AspS9I GGNCC 9 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667, 1686
AsuHPI GGTGA 2 cut(s) 670, 928
AsuII TTCGAA 1 cut(s) 285
AvaII GGWCC 8 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667
BaeI ACNNNNGTAYC 4 cut(s) 225, 258, 633, 666
BalI TGGCCA 2 cut(s) 126, 1486
BamHI GGATCC 1 cut(s) 65
BanI GGYRCC 1 cut(s) 1291
BanII GRGCYC 1 cut(s) 993
BauI CACGAG 2 cut(s) 1374, 1404
BbvI GCAGC 4 cut(s) 604, 1024, 1030, 1252
BccI CCATC 5 cut(s) 413, 1220, 1303, 1984, 2079
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 4 cut(s) 1673, 1807, 2001, 2176
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 5 cut(s) 830, 942, 1079, 1323, 1902
BfmI CTRYAG 2 cut(s) 819, 1506
BfrI CTTAAG 1 cut(s) 1007
BfuAI ACCTGC 3 cut(s) 1171, 1210, 1381
BglII AGATCT 2 cut(s) 190, 571
BisI GCNGC 5 cut(s) 618, 1013, 1044, 1266, 1916
BlpI GCTNAGC 1 cut(s) 1851
BlsI GCNGC 5 cut(s) 619, 1014, 1045, 1267, 1917
BmcAI AGTACT 1 cut(s) 2136
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 8 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667
BmgBI CACGTC 1 cut(s) 1683
BmgT120I GGNCC 9 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667, 1686
BmiI GGNNCC 4 cut(s) 67, 448, 1293, 1303
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 3 cut(s) 667, 946, 1463
BplI GAGNNNNNCTC 2 cut(s) 1805, 1837
Bpu1102I GCTNAGC 1 cut(s) 1851
Bpu14I TTCGAA 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 1765
Bsa29I ATCGAT 1 cut(s) 966
BsaI GGTCTC 1 cut(s) 1807
BsaJI CCNNGG 3 cut(s) 653, 892, 1357
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 1664, 1729
Bse118I RCCGGY 1 cut(s) 589
Bse1I ACTGG 5 cut(s) 69, 79, 1437, 1991, 2056
Bse3DI GCAATG 2 cut(s) 468, 1040
BseBI CCWGG 1 cut(s) 270
BseCI ATCGAT 1 cut(s) 966
BseDI CCNNGG 3 cut(s) 653, 892, 1357
BseGI GGATG 2 cut(s) 229, 1995
BseLI CCNNNNNNNGG 3 cut(s) 269, 1664, 1729
BseMI GCAATG 2 cut(s) 468, 1040
BseMII CTCAG 5 cut(s) 168, 364, 560, 1628, 1803
BseNI ACTGG 5 cut(s) 69, 79, 1437, 1991, 2056
BseRI GAGGAG 3 cut(s) 131, 1106, 1810
BseXI GCAGC 4 cut(s) 604, 1024, 1030, 1252
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 2 cut(s) 1898, 2101
Bsh1285I CGRYCG 1 cut(s) 633
BshFI GGCC 5 cut(s) 126, 1486, 1688, 1722, 1886
BshNI GGYRCC 1 cut(s) 1291
BshVI ATCGAT 1 cut(s) 966
BsiEI CGRYCG 1 cut(s) 633
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 1 cut(s) 1375
BslI CCNNNNNNNGG 3 cut(s) 269, 1664, 1729
BsmAI GTCTC 4 cut(s) 1673, 1807, 2001, 2176
BsmBI CGTCTC 1 cut(s) 1673
BsmFI GGGAC 1 cut(s) 1375
BsmI GAATGC 2 cut(s) 1162, 1218
BsnI GGCC 5 cut(s) 126, 1486, 1688, 1722, 1886
Bso31I GGTCTC 1 cut(s) 1807
Bsp119I TTCGAA 1 cut(s) 285
Bsp1286I GDGCHC 1 cut(s) 993
Bsp1720I GCTNAGC 1 cut(s) 1851
Bsp19I CCATGG 1 cut(s) 892
BspACI CCGC 6 cut(s) 629, 864, 918, 1916, 2013, 2099
BspANI GGCC 5 cut(s) 126, 1486, 1688, 1722, 1886
BspCNI CTCAG 5 cut(s) 169, 363, 559, 1629, 1804
BspDI ATCGAT 1 cut(s) 966
BspFNI CGCG 2 cut(s) 1898, 2101
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 4 cut(s) 67, 448, 1293, 1303
BspMAI CTGCAG 1 cut(s) 1510
BspMI ACCTGC 3 cut(s) 1171, 1210, 1381
BspPI GGATC 9 cut(s) 25, 60, 70, 73, 834, 1055, 1558, 1841, 1935
BspT104I TTCGAA 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 1291
BspTI CTTAAG 1 cut(s) 1007
BspTNI GGTCTC 1 cut(s) 1807
BsrDI GCAATG 2 cut(s) 468, 1040
BsrFI RCCGGY 1 cut(s) 589
BsrI ACTGG 5 cut(s) 69, 79, 1437, 1991, 2056
BssAI RCCGGY 1 cut(s) 589
BssECI CCNNGG 3 cut(s) 653, 892, 1357
BssSI CACGAG 2 cut(s) 1374, 1404
BssT1I CCWWGG 1 cut(s) 892
Bst2BI CACGAG 2 cut(s) 1374, 1404
Bst2UI CCWGG 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 2177
BstAFI CTTAAG 1 cut(s) 1007
BstAPI GCANNNNNTGC 2 cut(s) 1291, 1514
BstBI TTCGAA 1 cut(s) 285
BstC8I GCNNGC 5 cut(s) 1114, 1515, 1920, 1953, 2072
BstDEI CTNAG 7 cut(s) 177, 350, 546, 1048, 1637, 1812, 1851
BstDSI CCRYGG 3 cut(s) 653, 892, 1357
BstENI CCTNNNNNAGG 1 cut(s) 1727
BstF5I GGATG 2 cut(s) 229, 1995
BstFNI CGCG 2 cut(s) 1898, 2101
BstHHI GCGC 1 cut(s) 1900
BstMAI GTCTC 4 cut(s) 1673, 1807, 2001, 2176
BstMCI CGRYCG 1 cut(s) 633
BstMWI GCNNNNNNNGC 4 cut(s) 626, 1262, 1291, 1514
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 4 cut(s) 889, 1070, 1283, 1517
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 2 cut(s) 819, 1506
BstUI CGCG 2 cut(s) 1898, 2101
BstV1I GCAGC 4 cut(s) 604, 1024, 1030, 1252
BstX2I RGATCY 7 cut(s) 30, 65, 190, 571, 826, 1550, 1927
BstXI CCANNNNNNTGG 1 cut(s) 759
BstYI RGATCY 7 cut(s) 30, 65, 190, 571, 826, 1550, 1927
Bsu15I ATCGAT 1 cut(s) 966
BsuRI GGCC 5 cut(s) 126, 1486, 1688, 1722, 1886
BsuTUI ATCGAT 1 cut(s) 966
BtgI CCRYGG 3 cut(s) 653, 892, 1357
BtrI CACGTC 1 cut(s) 1683
BtsCI GGATG 2 cut(s) 229, 1995
BtsI GCAGTG 2 cut(s) 1107, 1251
BtsIMutI CAGTG 6 cut(s) 76, 86, 1107, 1251, 1444, 1503
BveI ACCTGC 3 cut(s) 1171, 1210, 1381
Cac8I GCNNGC 5 cut(s) 1114, 1515, 1920, 1953, 2072
CciI TCATGA 1 cut(s) 256
CfoI GCGC 1 cut(s) 1900
Cfr10I RCCGGY 1 cut(s) 589
Cfr13I GGNCC 9 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667, 1686
ClaI ATCGAT 1 cut(s) 966
CseI GACGC 3 cut(s) 507, 921, 1904
Csp6I GTAC 7 cut(s) 650, 889, 997, 1312, 1429, 1879, 2135
CspCI CAANNNNNGTGG 2 cut(s) 1422, 1457
CviQI GTAC 7 cut(s) 650, 889, 997, 1312, 1429, 1879, 2135
DdeI CTNAG 7 cut(s) 177, 350, 546, 1048, 1637, 1812, 1851
DraI TTTAAA 1 cut(s) 639
DraIII CACNNNGTG 1 cut(s) 527
DrdI GACNNNNNNGTC 1 cut(s) 594
DseDI GACNNNNNNGTC 1 cut(s) 594
EaeI YGGCCR 2 cut(s) 124, 1484
Eam1104I CTCTTC 1 cut(s) 2177
EarI CTCTTC 1 cut(s) 2177
Eco130I CCWWGG 1 cut(s) 892
Eco147I AGGCCT 1 cut(s) 1886
Eco24I GRGCYC 1 cut(s) 993
Eco31I GGTCTC 1 cut(s) 1807
Eco47I GGWCC 8 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667
EcoNI CCTNNNNNAGG 1 cut(s) 1727
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 1 cut(s) 892
Esp3I CGTCTC 1 cut(s) 1673
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 1 cut(s) 1375
Fnu4HI GCNGC 5 cut(s) 618, 1013, 1044, 1266, 1916
FokI GGATG 2 cut(s) 236, 2002
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 5 cut(s) 618, 1013, 1044, 1266, 1916
FspBI CTAG 5 cut(s) 830, 942, 1079, 1323, 1902
GlaI GCGC 1 cut(s) 1899
GluI GCNGC 5 cut(s) 618, 1013, 1044, 1266, 1916
HaeIII GGCC 5 cut(s) 126, 1486, 1688, 1722, 1886
HapII CCGG 1 cut(s) 590
HgaI GACGC 3 cut(s) 507, 921, 1904
HhaI GCGC 1 cut(s) 1900
Hin6I GCGC 1 cut(s) 1898
HinP1I GCGC 1 cut(s) 1898
HindIII AAGCTT 1 cut(s) 1953
HpaII CCGG 1 cut(s) 590
HphI GGTGA 2 cut(s) 670, 928
Hpy166II GTNNAC 6 cut(s) 530, 762, 786, 1107, 1431, 2194
Hpy188I TCNGA 6 cut(s) 353, 434, 492, 549, 1060, 1459
Hpy188III TCNNGA 5 cut(s) 176, 247, 257, 326, 1226
Hpy8I GTNNAC 6 cut(s) 530, 762, 786, 1107, 1431, 2194
Hpy99I CGWCG 2 cut(s) 1261, 1703
HpyAV CCTTC 3 cut(s) 273, 281, 460
HpyCH4IV ACGT 2 cut(s) 1239, 1682
HpyF10VI GCNNNNNNNGC 4 cut(s) 626, 1262, 1291, 1514
HpyF3I CTNAG 7 cut(s) 177, 350, 546, 1048, 1637, 1812, 1851
HpySE526I ACGT 2 cut(s) 1239, 1682
HspAI GCGC 1 cut(s) 1898
LmnI GCTCC 4 cut(s) 964, 1387, 1598, 1616
Lsp1109I GCAGC 4 cut(s) 604, 1024, 1030, 1252
LweI GCATC 3 cut(s) 667, 946, 1463
MaeI CTAG 5 cut(s) 830, 942, 1079, 1323, 1902
MaeII ACGT 2 cut(s) 1239, 1682
MaeIII GTNAC 7 cut(s) 91, 519, 596, 1069, 1399, 1733, 2021
MboII GAAGA 9 cut(s) 200, 303, 493, 683, 1047, 1214, 1346, 2156, 2194
MfeI CAATTG 1 cut(s) 610
MflI RGATCY 7 cut(s) 30, 65, 190, 571, 826, 1550, 1927
MhlI GDGCHC 1 cut(s) 993
MlsI TGGCCA 2 cut(s) 126, 1486
MluCI AATT 5 cut(s) 280, 354, 382, 610, 690
MluNI TGGCCA 2 cut(s) 126, 1486
MlyI GAGTC 1 cut(s) 1811
MmeI TCCRAC 2 cut(s) 1365, 1645
Mox20I TGGCCA 2 cut(s) 126, 1486
MroXI GAANNNNTTC 1 cut(s) 1582
MscI TGGCCA 2 cut(s) 126, 1486
MseI TTAA 7 cut(s) 315, 576, 638, 1008, 1139, 1395, 1449
MslI CAYNNNNRTG 3 cut(s) 603, 1155, 2083
Msp20I TGGCCA 2 cut(s) 126, 1486
MspA1I CMGCKG 1 cut(s) 1918
MspCI CTTAAG 1 cut(s) 1007
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 1 cut(s) 270
MssI GTTTAAAC 1 cut(s) 639
MunI CAATTG 1 cut(s) 610
Mva1269I GAATGC 2 cut(s) 1162, 1218
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 2 cut(s) 1898, 2101
MwoI GCNNNNNNNGC 4 cut(s) 626, 1262, 1291, 1514
NcoI CCATGG 1 cut(s) 892
NlaIV GGNNCC 4 cut(s) 67, 448, 1293, 1303
NmuCI GTSAC 2 cut(s) 519, 596
NspI RCATGY 4 cut(s) 889, 1070, 1283, 1517
NspV TTCGAA 1 cut(s) 285
PaeI GCATGC 1 cut(s) 1517
PagI TCATGA 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 1171
PceI AGGCCT 1 cut(s) 1886
PciI ACATGT 3 cut(s) 885, 1066, 1279
PcsI WCGNNNNNNNCGW 1 cut(s) 650
PctI GAATGC 2 cut(s) 1162, 1218
PdmI GAANNNNTTC 1 cut(s) 1582
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 5 cut(s) 619, 1014, 1045, 1267, 1917
PleI GAGTC 1 cut(s) 1811
PmeI GTTTAAAC 1 cut(s) 639
PpsI GAGTC 1 cut(s) 1811
PscI ACATGT 3 cut(s) 885, 1066, 1279
PsiI TTATAA 2 cut(s) 197, 695
Psp1406I AACGTT 1 cut(s) 1239
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 4 cut(s) 67, 448, 1293, 1303
PspPI GGNCC 9 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667, 1686
PstI CTGCAG 1 cut(s) 1510
PsuI RGATCY 7 cut(s) 30, 65, 190, 571, 826, 1550, 1927
RsaI GTAC 7 cut(s) 651, 890, 998, 1313, 1430, 1880, 2136
RsaNI GTAC 7 cut(s) 650, 889, 997, 1312, 1429, 1879, 2135
RseI CAYNNNNRTG 3 cut(s) 603, 1155, 2083
SaqAI TTAA 7 cut(s) 315, 576, 638, 1008, 1139, 1395, 1449
SatI GCNGC 5 cut(s) 618, 1013, 1044, 1266, 1916
Sau96I GGNCC 9 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667, 1686
ScaI AGTACT 1 cut(s) 2136
SchI GAGTC 1 cut(s) 1811
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 3 cut(s) 667, 946, 1463
SfcI CTRYAG 2 cut(s) 819, 1506
SfuI TTCGAA 1 cut(s) 285
SinI GGWCC 8 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667
SmiMI CAYNNNNRTG 3 cut(s) 603, 1155, 2083
SmlI CTYRAG 2 cut(s) 1007, 1744
SmoI CTYRAG 2 cut(s) 1007, 1744
SpeI ACTAGT 1 cut(s) 941
SphI GCATGC 1 cut(s) 1517
Sse9I AATT 5 cut(s) 280, 354, 382, 610, 690
SseBI AGGCCT 1 cut(s) 1886
SsiI CCGC 6 cut(s) 629, 864, 918, 1916, 2013, 2099
SspMI CTAG 5 cut(s) 830, 942, 1079, 1323, 1902
StuI AGGCCT 1 cut(s) 1886
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 892
TaiI ACGT 2 cut(s) 1242, 1685
TaqI TCGA 2 cut(s) 285, 966
TasI AATT 5 cut(s) 280, 354, 382, 610, 690
TatI WGTACW 3 cut(s) 996, 1311, 2134
TauI GCSGC 1 cut(s) 1918
Tru1I TTAA 7 cut(s) 315, 576, 638, 1008, 1139, 1395, 1449
Tru9I TTAA 7 cut(s) 315, 576, 638, 1008, 1139, 1395, 1449
TscAI CASTG 6 cut(s) 76, 86, 1114, 1258, 1444, 1503
TseFI GTSAC 2 cut(s) 519, 596
TseI GCWGC 4 cut(s) 617, 1012, 1043, 1265
Tsp45I GTSAC 2 cut(s) 519, 596
TspGWI ACGGA 2 cut(s) 723, 1563
TspRI CASTG 6 cut(s) 76, 86, 1114, 1258, 1444, 1503
Vha464I CTTAAG 1 cut(s) 1007
VpaK11BI GGWCC 8 cut(s) 359, 446, 530, 587, 926, 982, 1209, 1667
XagI CCTNNNNNAGG 1 cut(s) 1727
XapI RAATTY 1 cut(s) 382
XceI RCATGY 4 cut(s) 889, 1070, 1283, 1517
XcmI CCANNNNNNNNNTGG 1 cut(s) 2063
XmnI GAANNNNTTC 1 cut(s) 1582
XspI CTAG 5 cut(s) 830, 942, 1079, 1323, 1902
ZrmI AGTACT 1 cut(s) 2136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.