Rorug06G0310300

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009802
Physical Location & Seq
Forward (+)
46161095 .. 46162209
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0310300.1

Sequence Viewer

Length: 687 bp
ATGACTGGCTCTGGTTCTCCTTGTGGAGCCTGCAAGTTCTTGAGAAGAAAATGTGTGAGGGGTTGTATTTTTGCACCTTATTTCTGCCATGAAAATGGCGCTACCCATTTTGCTGCCATCCATAAGGTTTTCGGCGCAAGCAATGTGTCGAAGCTGCTGGCTCACCTTCCGGTGAGTGATCGTTGTGAAGCTGCAGTGACTATCTCATATGAAGCTCAGGCCAGGCTCCAAGATCCCATTTATGGCTGTGTTTCACATATTTTTGCTCTCCAACAACAGGTTGTCAACCTCCAGGCACAATTAGCTTCCCTCAAGGATCAAGCACCTCAAAGCTTTGTGAATGGCTCTGCAGCTGTAGCAAACCCTAACGAGAATTGGTTTCCTGATTTAGAGAATTCATCTAACTCAGTGGCCCAAGTCAATTCAAATTACCATATCAATAATGGTGGAACAACATCATACTATGAGAACGGAATTATGAGTTCAAACTCCATTGGGAGTAGTAATTATGATCAAAACTCAGGAATCCGGGATGAAAATGCATCCTATGGAAGCTTTGAAGAGGTGTCTCCTTCCCTGTCTTCTTTCAACATGCAAATGAATAACAGGCAATATTGGGGTAATTATGATCGGGACGTGGATGAGCTTCAATCTATGACTTTCAGCTATGTTGATCAGCATTCTTGA

Protein Analysis

228

Amino Acids

25.04

Weight (kDa)

5.39

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 7 - 105 3.8e-35 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 227, 324
AcsI RAATTY 1 cut(s) 394
AfiI CCNNNNNNNGG 2 cut(s) 242, 277
AgsI TTSAA 5 cut(s) 426, 486, 560, 589, 650
AjiI CACGTC 1 cut(s) 637
AjnI CCWGG 2 cut(s) 221, 291
AluBI AGCT 9 cut(s) 154, 191, 215, 305, 333, 353, 555, 646, 666
AluI AGCT 9 cut(s) 154, 191, 215, 305, 333, 353, 555, 646, 666
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 2 cut(s) 227, 324
AoxI GGCC 2 cut(s) 219, 411
ApeKI GCWGC 4 cut(s) 113, 154, 191, 350
ApoI RAATTY 1 cut(s) 394
AspLEI GCGC 2 cut(s) 101, 137
AspS9I GGNCC 1 cut(s) 412
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 2 cut(s) 155, 184
BbsI GAAGAC 1 cut(s) 573
BbvI GCAGC 4 cut(s) 100, 141, 178, 362
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 2 cut(s) 223, 293
BclI TGATCA 2 cut(s) 511, 673
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 573
BfmI CTRYAG 3 cut(s) 192, 348, 354
BfoI RGCGCY 1 cut(s) 102
BisI GCNGC 4 cut(s) 114, 155, 192, 351
BlsI GCNGC 4 cut(s) 115, 156, 193, 352
Bme1390I CCNGG 3 cut(s) 223, 293, 530
BmgBI CACGTC 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 28, 227
BmrFI CCNGG 3 cut(s) 223, 293, 530
BmsI GCATC 1 cut(s) 551
BpiI GAAGAC 1 cut(s) 573
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 2 cut(s) 61, 296
BpuMI CCSGG 1 cut(s) 530
BsaWI WCCGGW 1 cut(s) 169
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 277
Bse1I ACTGG 1 cut(s) 10
Bse3DI GCAATG 1 cut(s) 148
BseBI CCWGG 2 cut(s) 223, 293
BseGI GGATG 4 cut(s) 117, 538, 542, 646
BseLI CCNNNNNNNGG 2 cut(s) 242, 277
BseMI GCAATG 1 cut(s) 148
BseMII CTCAG 3 cut(s) 230, 420, 534
BseNI ACTGG 1 cut(s) 10
BseXI GCAGC 4 cut(s) 100, 141, 178, 362
BshFI GGCC 2 cut(s) 221, 413
BsiSI CCGG 2 cut(s) 170, 529
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 2 cut(s) 242, 277
BsmAI GTCTC 1 cut(s) 573
BsmFI GGGAC 1 cut(s) 647
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 2 cut(s) 221, 413
Bsp143I GATC 6 cut(s) 178, 232, 316, 511, 628, 673
BspANI GGCC 2 cut(s) 221, 413
BspCNI CTCAG 3 cut(s) 229, 419, 533
BspLI GGNNCC 2 cut(s) 28, 227
BspMAI CTGCAG 2 cut(s) 196, 352
BspPI GGATC 2 cut(s) 227, 324
BsrDI GCAATG 1 cut(s) 148
BsrI ACTGG 1 cut(s) 10
BssMI GATC 6 cut(s) 178, 232, 316, 511, 628, 673
Bst2UI CCWGG 2 cut(s) 223, 293
Bst6I CTCTTC 1 cut(s) 555
BstC8I GCNNGC 3 cut(s) 31, 139, 159
BstDEI CTNAG 3 cut(s) 216, 406, 520
BstF5I GGATG 4 cut(s) 117, 538, 542, 646
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 101, 137
BstKTI GATC 6 cut(s) 181, 235, 319, 514, 631, 676
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 6 cut(s) 178, 232, 316, 511, 628, 673
BstMWI GCNNNNNNNGC 2 cut(s) 302, 356
BstNI CCWGG 2 cut(s) 223, 293
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 3 cut(s) 221, 291, 528
BstSFI CTRYAG 3 cut(s) 192, 348, 354
BstV1I GCAGC 4 cut(s) 100, 141, 178, 362
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 1 cut(s) 232
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 1 cut(s) 232
BsuRI GGCC 2 cut(s) 221, 413
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 4 cut(s) 117, 538, 542, 646
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 201, 414
Cac8I GCNNGC 3 cut(s) 31, 139, 159
CfoI GCGC 2 cut(s) 101, 137
Cfr13I GGNCC 1 cut(s) 412
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 2 cut(s) 89, 592
DdeI CTNAG 3 cut(s) 216, 406, 520
DpnI GATC 6 cut(s) 180, 234, 318, 513, 630, 675
DpnII GATC 6 cut(s) 178, 232, 316, 511, 628, 673
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 221, 291
EcoT22I ATGCAT 1 cut(s) 544
FaeI CATG 2 cut(s) 92, 595
FaqI GGGAC 1 cut(s) 647
FatI CATG 2 cut(s) 88, 591
FauNDI CATATG 1 cut(s) 208
FbaI TGATCA 2 cut(s) 511, 673
Fnu4HI GCNGC 4 cut(s) 114, 155, 192, 351
FokI GGATG 4 cut(s) 104, 529, 545, 653
Fsp4HI GCNGC 4 cut(s) 114, 155, 192, 351
GlaI GCGC 2 cut(s) 100, 136
GluI GCNGC 4 cut(s) 114, 155, 192, 351
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 2 cut(s) 221, 413
HapII CCGG 2 cut(s) 170, 529
HhaI GCGC 2 cut(s) 101, 137
Hin1II CATG 2 cut(s) 92, 595
Hin6I GCGC 2 cut(s) 99, 135
HinP1I GCGC 2 cut(s) 99, 135
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HindIII AAGCTT 2 cut(s) 331, 553
HinfI GANTC 1 cut(s) 525
HpaII CCGG 2 cut(s) 170, 529
HphI GGTGA 2 cut(s) 155, 184
Hpy166II GTNNAC 1 cut(s) 286
Hpy188III TCNNGA 5 cut(s) 40, 383, 522, 632, 684
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 2 cut(s) 176, 582
HpyCH4IV ACGT 1 cut(s) 636
HpyCH4V TGCA 6 cut(s) 33, 74, 194, 350, 542, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 356
HpyF3I CTNAG 3 cut(s) 216, 406, 520
HpySE526I ACGT 1 cut(s) 636
Hsp92II CATG 2 cut(s) 92, 595
HspAI GCGC 2 cut(s) 99, 135
Ksp22I TGATCA 2 cut(s) 511, 673
Kzo9I GATC 6 cut(s) 178, 232, 316, 511, 628, 673
LmnI GCTCC 2 cut(s) 26, 231
Lsp1109I GCAGC 4 cut(s) 100, 141, 178, 362
LweI GCATC 1 cut(s) 551
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 1 cut(s) 196
MalI GATC 6 cut(s) 180, 234, 318, 513, 630, 675
MboI GATC 6 cut(s) 178, 232, 316, 511, 628, 673
MboII GAAGA 3 cut(s) 57, 572, 573
MflI RGATCY 1 cut(s) 232
MluCI AATT 8 cut(s) 299, 373, 394, 421, 427, 474, 505, 622
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 5 cut(s) 51, 299, 320, 336, 556
Mph1103I ATGCAT 1 cut(s) 544
MslI CAYNNNNRTG 2 cut(s) 93, 596
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 170, 529
MspR9I CCNGG 3 cut(s) 223, 293, 530
Mva1269I GAATGC 1 cut(s) 679
MvaI CCWGG 2 cut(s) 223, 293
MwoI GCNNNNNNNGC 2 cut(s) 302, 356
NciI CCSGG 1 cut(s) 530
NdeI CATATG 1 cut(s) 208
NdeII GATC 6 cut(s) 178, 232, 316, 511, 628, 673
NlaIII CATG 2 cut(s) 92, 595
NlaIV GGNNCC 2 cut(s) 28, 227
NmuCI GTSAC 1 cut(s) 196
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 595
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 1 cut(s) 525
PfoI TCCNGGA 1 cut(s) 528
PkrI GCNGC 4 cut(s) 115, 156, 193, 352
Psp6I CCWGG 2 cut(s) 221, 291
PspGI CCWGG 2 cut(s) 221, 291
PspN4I GGNNCC 2 cut(s) 28, 227
PspPI GGNCC 1 cut(s) 412
PstI CTGCAG 2 cut(s) 196, 352
PsuI RGATCY 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 353
RseI CAYNNNNRTG 2 cut(s) 93, 596
SatI GCNGC 4 cut(s) 114, 155, 192, 351
Sau3AI GATC 6 cut(s) 178, 232, 316, 511, 628, 673
Sau96I GGNCC 1 cut(s) 412
ScrFI CCNGG 3 cut(s) 223, 293, 530
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 3 cut(s) 192, 348, 354
SmiMI CAYNNNNRTG 2 cut(s) 93, 596
SmlI CTYRAG 2 cut(s) 40, 311
SmoI CTYRAG 2 cut(s) 40, 311
Sse9I AATT 8 cut(s) 299, 373, 394, 421, 427, 474, 505, 622
SspI AATATT 1 cut(s) 614
StyD4I CCNGG 3 cut(s) 221, 291, 528
TaiI ACGT 1 cut(s) 639
TaqI TCGA 1 cut(s) 149
TasI AATT 8 cut(s) 299, 373, 394, 421, 427, 474, 505, 622
TfiI GAWTC 1 cut(s) 525
TscAI CASTG 2 cut(s) 201, 414
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 4 cut(s) 113, 154, 191, 350
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 5 cut(s) 105, 225, 387, 549, 614
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 2 cut(s) 201, 414
XapI RAATTY 1 cut(s) 394
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.