MD15G1021100.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009802
Physical Location & Seq
Reverse (-)
1216768 .. 1221862
5095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1021100.v1.1.491

Sequence Viewer

Length: 2559 bp
ATGGAAACTCACTCGGTTTCCAAGCTCTTAGTTTTTTTCATGTCGATGGCATTGTTCTTGGGTTCTGAGCTTATCCACTGCACCACCGTCACCTACGACAAGAAGGCCATTCTCATCAATGGCCAGAGAAGATTGCTCATTTCTGGATCCATTCATTATCCCAGAAGTACCCCTGAAATGTGGGAAGGCCTCATACAGAAAGCAAAAGATGGAGGCTTGGATGTGATCGACACCTACGTTTTCTGGAACGGCCATGAACCTTCTCCTGGCAATTATTATTTTGAGGGGAGATACGATCTAGTAGGGTTCATAAAGACAGTACAAAAAGCAGGGCTCTTTCTTCATCTGCGCATCGGGCCTTATATTTGTGCAGAGTGGAATTTTGGAGGATTTCCCGTTTGGCTGAAATATGTACCTGGCATCAGTTTCAGGACAGATAATGGGCCGTTTAAGATGGCAATGCAAGGGTTCACCCAGAAGATTGTCCAGATGATGAAGGATGAGAAGCTATTTGCATCACAAGGTGGTCCCATCATCCTTTCCCAGATTGAGAATGAGTATGGACCGGAGAGCAAGGCACTGGGAGCTGCTGGCCATAACTACATTAACTGGGCTGCTCAAATGGCGGTTGGATTGGATACCGGAGTCCCATGGGTGATGTGCAAGGAAGACGATGCCCCAGACCCTATGATAAATGCATGCAACGGTTTTTACTGCGATGGATTCACTCCCAACAAACCTTACAAGCCTGCCATGTGGACGGAAGCTTGGAGCGGCTGGTTTACAGAGTTTGGTGGCACAATTCACCATCGACCTGTCCAAGATTTGGCATTCGCAGTTGGTCGATTCATACAGAAGGGTGGCTCATATGTGAATTACTACATGTATCATGGAGGAACAAACTTCGGACGAACAGCTGGAGGCCCCTTCATTACAACCAGTTACGACTATGATGCTCCCATTGATGAATACGGTTTGATCAGAGAACCTAAATATGGTCATCTGAAGGAGCTTCACAAGGCTATCAAGCTATGTGAACATTCTTTACTCTCTTCAGAGCCTACCGTCACTTCACTAGGAACCTATCATCAGGCTTATGTATTCAATTCAGGACCAAATAGATGCGCAGCGTTTCTCTCAAACTTCCATTCAACAGGAGCAAAAGTGACTTTTAATAACTTGCACTATGATTTGCCGCCTTGGTCTGTTAGCATCCTTCCTGATTGCAGGAATGAAGTATTCAACACTGCAAAGGTGGGAGTTCAAACGTCACATGTGCAGATGATCCCAACGAACAGTCTGTTGTTTTCATGGCAGACTTACGATGAAGATATCTCTTCTCTAAACGATGGGTCAAGCATACCGGCTGTTGGACTTCTAGAGCAGATAAATGTAACTAGAGATACTAGTGACTATCTGTGGTACATGACAAATGTTGACATCAGTTCATCAGACCTCGGAGGAGGAAAAAAGCCTACTCTTACTGTGCAGTCAGCAGGTCATGCCCTCCATGTCTTCGTCAACGGGCAGTTTTCAGGTTCGGCGTTTGGAACTAGGGAGCAGAGGCAATTCACATTTGCTGAACCAGTCAACCTCCGCACTGGAATAAACAGAATTGCACTGCTCAGCATAGCTGTTGGATTACCAAATGTCGGGTTGCATTACGAGTCGTGGAAAACGGGAATCCAAGGTCCGGTTTTTCTGGATGGTCTTGGCCACGGAAAGAAAGACTTAACGTCGCAGAAGTGGTTCTACAAGGTCGGCCTTAAGGGAGAAGAAATGAATTTGGTCTCTCCAAACGGAGCCTCATCAGTTGATTGGATCCGTAGGTCACTAGCTACCCAAACCAAGCAGACGTTGAAATGGTACAAGGCTTATTTCAATGCACCCGGAGGAAATGAGCCATTGGCGTTGGACATGAGTAGCATGGGAAAGGGACAAGTATGGATCAATGGGCAGAGCATCGGTAGATACTGGATGGCTTATGCCAAAGGTGACTGCAGTTCGTGCAGTTACATTGGGACATTCCGTCCCACGGAATGTCAACTTGGCTGTGGCCAACCAACTCAACGATGGTACCATGTTCCTCGTTCATGGTTAAAGCCGACGCAAAATTTGGTGGTAGTGTTTGAGGAACTAGGCGGAGATCCATCAAAGATAACCCTAGTGAAAAGATCTGTGACCGGTGTTTGTGGTGACTTACATGAGAACCACCCAAATGTCGAAAATTTTGATGTCGAGGGCAATGAAAATTCTAAAACGCTTCACCAAGCTCAGGTTCATCTGCAGTGTGCACCGGGGAAGTCTATCTCGAGCATTAAGTTTGCAAGTTTCGGAACTCCTACAGGAACTTGTGGGAGTTTCCAGCAAGGAACGTGTCACGCGAAAAACTCACACGGCGTGGTGGAGAAGAATTGTATCGGGCGTGAGAGTTGCTCGGTTGCTGTAACCAATAGCATTTTCAAGACAGATCCATGTCCGAATGTACTCAAACGGCTATCAGTTGAAGCTGTTTGTTCGACGGAAATTACAACCAATCAGCCCAATTCGAGGAGATGA

Protein Analysis

853

Amino Acids

94.31

Weight (kDa)

7.55

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 341 3.9e-121 Glycosyl hydrolases family 35
GHD PF17834 364 - 419 5e-22 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 472 - 535 2e-09 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 621 - 705 1.5e-27 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 762 - 839 1.6e-22 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 350, 1126
Acc36I ACCTGC 1 cut(s) 1487
Acc65I GGTACC 1 cut(s) 2076
AccB1I GGYRCC 1 cut(s) 2076
AccB7I CCANNNNNTGG 1 cut(s) 826
AccBSI CCGCTC 1 cut(s) 774
AccII CGCG 1 cut(s) 2384
AciI CCGC 5 cut(s) 626, 774, 1196, 1597, 2142
AclWI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
AcoI YGGCCR 5 cut(s) 121, 250, 592, 1714, 2056
AcsI RAATTY 5 cut(s) 379, 1783, 2113, 2227, 2251
AcuI CTGAAG 2 cut(s) 1025, 1038
AdeI CACNNNGTG 2 cut(s) 524, 2401
AfaI GTAC 7 cut(s) 169, 321, 414, 1424, 1868, 2078, 2487
AfiI CCNNNNNNNGG 9 cut(s) 266, 826, 995, 1370, 1652, 1693, 2035, 2275, 2550
AflII CTTAAG 1 cut(s) 1766
AflIII ACRYGT 3 cut(s) 882, 1273, 2375
AgeI ACCGGT 1 cut(s) 2183
AgsI TTSAA 8 cut(s) 1105, 1152, 1243, 1265, 1861, 1882, 2464, 2507
AhdI GACNNNNNGTC 2 cut(s) 1735, 2028
AhlI ACTAGT 1 cut(s) 1406
AjnI CCWGG 2 cut(s) 265, 415
AjuI GAANNNNNNNTTGG 6 cut(s) 2031, 2063, 2262, 2294, 2444, 2476
Alw21I GWGCWC 1 cut(s) 2296
Alw26I GTCTC 1 cut(s) 1795
Alw44I GTGCAC 1 cut(s) 2292
AlwI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
Ama87I CYCGRG 1 cut(s) 2311
ApaLI GTGCAC 1 cut(s) 2292
ApeKI GCWGC 3 cut(s) 587, 614, 1127
ApoI RAATTY 5 cut(s) 379, 1783, 2113, 2227, 2251
ArsI GACNNNNNNTTYG 2 cut(s) 2098, 2130
AsiGI ACCGGT 1 cut(s) 2183
Asp700I GAANNNNTTC 1 cut(s) 1748
Asp718I GGTACC 1 cut(s) 2076
AspLEI GCGC 2 cut(s) 351, 1127
AspS9I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
AsuC2I CCSGG 2 cut(s) 1890, 2298
AsuHPI GGTGA 7 cut(s) 82, 463, 667, 797, 2006, 2207, 2258
AvaI CYCGRG 1 cut(s) 2311
AvaII GGWCC 4 cut(s) 527, 563, 1112, 1691
BaeGI GKGCMC 1 cut(s) 2296
BaeI ACNNNNGTAYC 4 cut(s) 630, 663, 2060, 2093
BalI TGGCCA 4 cut(s) 123, 594, 1716, 2058
BamHI GGATCC 2 cut(s) 146, 1821
BanI GGYRCC 1 cut(s) 2076
BanII GRGCYC 1 cut(s) 336
BarI GAAGNNNNNNTAC 4 cut(s) 177, 209, 1736, 1768
BbsI GAAGAC 2 cut(s) 675, 1507
Bbv12I GWGCWC 1 cut(s) 2296
BbvI GCAGC 3 cut(s) 574, 601, 1139
BceAI ACGGC 4 cut(s) 265, 430, 2413, 2510
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 2 cut(s) 267, 417
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 2 cut(s) 1890, 2298
BcoDI GTCTC 1 cut(s) 1795
BcuI ACTAGT 1 cut(s) 1406
BfaI CTAG 9 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138, 2165
BfmI CTRYAG 3 cut(s) 1999, 2285, 2343
BfrI CTTAAG 1 cut(s) 1766
BfuAI ACCTGC 1 cut(s) 1487
BfuI GTATCC 1 cut(s) 631
BglII AGATCT 1 cut(s) 2174
BisI GCNGC 5 cut(s) 588, 615, 775, 1128, 1196
BlpI GCTNAGC 1 cut(s) 1625
BlsI GCNGC 5 cut(s) 589, 616, 776, 1129, 1197
Bme1390I CCNGG 4 cut(s) 267, 417, 1890, 2298
Bme18I GGWCC 4 cut(s) 527, 563, 1112, 1691
BmeRI GACNNNNNGTC 2 cut(s) 1735, 2028
BmeT110I CYCGRG 1 cut(s) 2311
BmgT120I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
BmiI GGNNCC 7 cut(s) 148, 529, 925, 1081, 1804, 1823, 2078
BmrFI CCNGG 4 cut(s) 267, 417, 1890, 2298
BmrI ACTGGG 2 cut(s) 590, 619
BmsI GCATC 8 cut(s) 360, 429, 524, 664, 943, 1112, 1221, 1971
BmuI ACTGGG 2 cut(s) 590, 619
BpiI GAAGAC 2 cut(s) 675, 1507
BplI GAGNNNNNCTC 2 cut(s) 2420, 2452
BpmI CTGGAG 1 cut(s) 939
Bpu10I CCTNAGC 1 cut(s) 2274
Bpu1102I GCTNAGC 1 cut(s) 1625
BpuMI CCSGG 2 cut(s) 1890, 2298
BsaI GGTCTC 1 cut(s) 1795
BsaJI CCNNGG 7 cut(s) 650, 1199, 1456, 1687, 1717, 2034, 2297
BsaWI WCCGGW 4 cut(s) 565, 641, 1693, 2183
BsaXI ACNNNNNCTCC 2 cut(s) 1149, 1179
Bsc4I CCNNNNNNNGG 9 cut(s) 266, 826, 995, 1370, 1652, 1693, 2035, 2275, 2550
Bse118I RCCGGY 2 cut(s) 1363, 2183
Bse1I ACTGG 6 cut(s) 585, 614, 939, 1586, 1606, 1979
Bse3DI GCAATG 2 cut(s) 465, 2251
BseBI CCWGG 2 cut(s) 267, 417
BseDI CCNNGG 7 cut(s) 650, 1199, 1456, 1687, 1717, 2034, 2297
BseGI GGATG 6 cut(s) 226, 505, 534, 1212, 1711, 1983
BseLI CCNNNNNNNGG 9 cut(s) 266, 826, 995, 1370, 1652, 1693, 2035, 2275, 2550
BseMI GCAATG 2 cut(s) 465, 2251
BseMII CTCAG 3 cut(s) 57, 1639, 2288
BseNI ACTGG 6 cut(s) 585, 614, 939, 1586, 1606, 1979
BseRI GAGGAG 1 cut(s) 1476
BseSI GKGCMC 1 cut(s) 2296
BseXI GCAGC 3 cut(s) 574, 601, 1139
BsgI GTGCAG 5 cut(s) 64, 390, 1298, 1508, 2029
Bsh1236I CGCG 1 cut(s) 2384
BshNI GGYRCC 1 cut(s) 2076
BshTI ACCGGT 1 cut(s) 2183
BsiHKAI GWGCWC 1 cut(s) 2296
BsiHKCI CYCGRG 1 cut(s) 2311
BsiSI CCGG 7 cut(s) 566, 642, 1364, 1694, 1890, 2184, 2297
BslFI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
BslI CCNNNNNNNGG 9 cut(s) 266, 826, 995, 1370, 1652, 1693, 2035, 2275, 2550
BsmAI GTCTC 1 cut(s) 1795
BsmFI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
BsmI GAATGC 1 cut(s) 830
Bso31I GGTCTC 1 cut(s) 1795
BsoBI CYCGRG 1 cut(s) 2311
Bsp1286I GDGCHC 2 cut(s) 336, 2296
Bsp1720I GCTNAGC 1 cut(s) 1625
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 5 cut(s) 626, 774, 1196, 1597, 2142
BspCNI CTCAG 3 cut(s) 58, 1638, 2287
BspFNI CGCG 1 cut(s) 2384
BspLI GGNNCC 7 cut(s) 148, 529, 925, 1081, 1804, 1823, 2078
BspMAI CTGCAG 2 cut(s) 2003, 2289
BspMI ACCTGC 1 cut(s) 1487
BspPI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
BspT107I GGYRCC 1 cut(s) 2076
BspTI CTTAAG 1 cut(s) 1766
BspTNI GGTCTC 1 cut(s) 1795
BsrBI CCGCTC 1 cut(s) 774
BsrDI GCAATG 2 cut(s) 465, 2251
BsrFI RCCGGY 2 cut(s) 1363, 2183
BsrI ACTGG 6 cut(s) 585, 614, 939, 1586, 1606, 1979
BssAI RCCGGY 2 cut(s) 1363, 2183
BssECI CCNNGG 7 cut(s) 650, 1199, 1456, 1687, 1717, 2034, 2297
BssT1I CCWWGG 3 cut(s) 650, 1199, 1687
Bst2UI CCWGG 2 cut(s) 267, 417
Bst4CI ACNGT 7 cut(s) 88, 319, 707, 974, 1066, 1298, 1486
Bst6I CTCTTC 2 cut(s) 1057, 1342
BstAFI CTTAAG 1 cut(s) 1766
BstAPI GCANNNNNTGC 2 cut(s) 1502, 2007
BstC8I GCNNGC 3 cut(s) 592, 700, 750
BstDEI CTNAG 4 cut(s) 28, 66, 1625, 2274
BstDSI CCRYGG 3 cut(s) 650, 1717, 2034
BstF5I GGATG 6 cut(s) 226, 505, 534, 1212, 1711, 1983
BstFNI CGCG 1 cut(s) 2384
BstHHI GCGC 2 cut(s) 351, 1127
BstMAI GTCTC 1 cut(s) 1795
BstMWI GCNNNNNNNGC 5 cut(s) 355, 584, 623, 1502, 2007
BstNI CCWGG 2 cut(s) 267, 417
BstNSI RCATGY 3 cut(s) 702, 886, 1277
BstSCI CCNGG 4 cut(s) 265, 415, 1888, 2296
BstSFI CTRYAG 3 cut(s) 1999, 2285, 2343
BstSLI GKGCMC 1 cut(s) 2296
BstUI CGCG 1 cut(s) 2384
BstV1I GCAGC 3 cut(s) 574, 601, 1139
BstV2I GAAGAC 2 cut(s) 675, 1507
BstX2I RGATCY 5 cut(s) 146, 1821, 2146, 2174, 2470
BstYI RGATCY 5 cut(s) 146, 1821, 2146, 2174, 2470
BsuI GTATCC 1 cut(s) 631
BtgI CCRYGG 3 cut(s) 650, 1717, 2034
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 6 cut(s) 226, 505, 534, 1212, 1711, 1983
BtsI GCAGTG 4 cut(s) 76, 1245, 1619, 2294
BtsIMutI CAGTG 6 cut(s) 76, 578, 1245, 1599, 1619, 2294
BveI ACCTGC 1 cut(s) 1487
Cac8I GCNNGC 3 cut(s) 592, 700, 750
CfoI GCGC 2 cut(s) 351, 1127
Cfr10I RCCGGY 2 cut(s) 1363, 2183
Cfr13I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
CseI GACGC 1 cut(s) 2116
Csp6I GTAC 7 cut(s) 168, 320, 413, 1423, 1867, 2077, 2486
CspAI ACCGGT 1 cut(s) 2183
CviQI GTAC 7 cut(s) 168, 320, 413, 1423, 1867, 2077, 2486
DdeI CTNAG 4 cut(s) 28, 66, 1625, 2274
DraIII CACNNNGTG 2 cut(s) 524, 2401
DriI GACNNNNNGTC 2 cut(s) 1735, 2028
EaeI YGGCCR 5 cut(s) 121, 250, 592, 1714, 2056
Eam1104I CTCTTC 2 cut(s) 1057, 1342
Eam1105I GACNNNNNGTC 2 cut(s) 1735, 2028
EarI CTCTTC 2 cut(s) 1057, 1342
EciI GGCGGA 1 cut(s) 2157
Eco130I CCWWGG 3 cut(s) 650, 1199, 1687
Eco147I AGGCCT 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 1795
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 4 cut(s) 527, 563, 1112, 1691
Eco57I CTGAAG 2 cut(s) 1025, 1038
Eco88I CYCGRG 1 cut(s) 2311
EcoO109I RGGNCCY 1 cut(s) 923
EcoRII CCWGG 2 cut(s) 265, 415
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 3 cut(s) 650, 1199, 1687
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 3 cut(s) 650, 1199, 1687
FalI AAGNNNNNCTT 2 cut(s) 1715, 1747
FaqI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
FauNDI CATATG 1 cut(s) 868
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 5 cut(s) 588, 615, 775, 1128, 1196
FokI GGATG 6 cut(s) 233, 512, 521, 1199, 1718, 1990
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 5 cut(s) 588, 615, 775, 1128, 1196
FspBI CTAG 9 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138, 2165
FspI TGCGCA 2 cut(s) 350, 1126
GlaI GCGC 2 cut(s) 350, 1126
GluI GCNGC 5 cut(s) 588, 615, 775, 1128, 1196
GsuI CTGGAG 1 cut(s) 939
HapII CCGG 7 cut(s) 566, 642, 1364, 1694, 1890, 2184, 2297
HgaI GACGC 1 cut(s) 2116
HhaI GCGC 2 cut(s) 351, 1127
Hin6I GCGC 2 cut(s) 349, 1125
HinP1I GCGC 2 cut(s) 349, 1125
HincII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 5 cut(s) 645, 723, 846, 1667, 1683
HpaII CCGG 7 cut(s) 566, 642, 1364, 1694, 1890, 2184, 2297
HphI GGTGA 7 cut(s) 82, 463, 667, 797, 2006, 2207, 2258
Hpy166II GTNNAC 9 cut(s) 471, 759, 783, 1037, 1438, 1522, 1591, 2045, 2294
Hpy188I TCNGA 9 cut(s) 67, 908, 983, 1005, 1057, 1453, 1460, 2336, 2481
Hpy8I GTNNAC 9 cut(s) 471, 759, 783, 1037, 1438, 1522, 1591, 2045, 2294
Hpy99I CGWCG 3 cut(s) 1741, 2110, 2524
HpyAV CCTTC 8 cut(s) 97, 179, 270, 490, 850, 937, 1000, 1226
HpyCH4III ACNGT 7 cut(s) 88, 319, 707, 974, 1066, 1298, 1486
HpyCH4IV ACGT 5 cut(s) 237, 1268, 1736, 1856, 2375
HpyF10VI GCNNNNNNNGC 5 cut(s) 355, 584, 623, 1502, 2007
HpyF3I CTNAG 4 cut(s) 28, 66, 1625, 2274
HpySE526I ACGT 5 cut(s) 237, 1268, 1736, 1856, 2375
HspAI GCGC 2 cut(s) 349, 1125
KpnI GGTACC 1 cut(s) 2080
Ksp22I TGATCA 1 cut(s) 978
LmnI GCTCC 7 cut(s) 584, 771, 961, 1009, 1157, 1558, 1802
Lsp1109I GCAGC 3 cut(s) 574, 601, 1139
LweI GCATC 8 cut(s) 360, 429, 524, 664, 943, 1112, 1221, 1971
MaeI CTAG 9 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138, 2165
MaeII ACGT 5 cut(s) 237, 1268, 1736, 1856, 2375
MbiI CCGCTC 1 cut(s) 774
MflI RGATCY 5 cut(s) 146, 1821, 2146, 2174, 2470
MhlI GDGCHC 2 cut(s) 336, 2296
MlsI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MluNI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MlyI GAGTC 2 cut(s) 654, 1676
MmeI TCCRAC 4 cut(s) 610, 1351, 1618, 1893
Mox20I TGGCCA 4 cut(s) 123, 594, 1716, 2058
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 1748
MscI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MseI TTAA 7 cut(s) 450, 606, 1173, 1733, 1767, 2099, 2319
MslI CAYNNNNRTG 2 cut(s) 44, 2217
Msp20I TGGCCA 4 cut(s) 123, 594, 1716, 2058
MspA1I CMGCKG 1 cut(s) 917
MspCI CTTAAG 1 cut(s) 1766
MspI CCGG 7 cut(s) 566, 642, 1364, 1694, 1890, 2184, 2297
MspR9I CCNGG 4 cut(s) 267, 417, 1890, 2298
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 2 cut(s) 267, 417
MvnI CGCG 1 cut(s) 2384
MwoI GCNNNNNNNGC 5 cut(s) 355, 584, 623, 1502, 2007
NciI CCSGG 2 cut(s) 1890, 2298
NcoI CCATGG 1 cut(s) 650
NdeI CATATG 1 cut(s) 868
NlaIV GGNNCC 7 cut(s) 148, 529, 925, 1081, 1804, 1823, 2078
NsbI TGCGCA 2 cut(s) 350, 1126
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 3 cut(s) 702, 886, 1277
PaeI GCATGC 1 cut(s) 702
PaeR7I CTCGAG 1 cut(s) 2311
PceI AGGCCT 1 cut(s) 189
PciI ACATGT 2 cut(s) 882, 1273
PcsI WCGNNNNNNNCGW 2 cut(s) 234, 2381
PctI GAATGC 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 1748
PfeI GAWTC 3 cut(s) 723, 846, 1683
PflMI CCANNNNNTGG 1 cut(s) 826
PinAI ACCGGT 1 cut(s) 2183
PkrI GCNGC 5 cut(s) 589, 616, 776, 1129, 1197
PleI GAGTC 2 cut(s) 653, 1675
PpsI GAGTC 2 cut(s) 653, 1675
PscI ACATGT 2 cut(s) 882, 1273
Psp6I CCWGG 2 cut(s) 265, 415
PspGI CCWGG 2 cut(s) 265, 415
PspN4I GGNNCC 7 cut(s) 148, 529, 925, 1081, 1804, 1823, 2078
PspPI GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
PsrI GAACNNNNNNTAC 2 cut(s) 1029, 1061
PstI CTGCAG 2 cut(s) 2003, 2289
PsuI RGATCY 5 cut(s) 146, 1821, 2146, 2174, 2470
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 7 cut(s) 169, 321, 414, 1424, 1868, 2078, 2487
RsaNI GTAC 7 cut(s) 168, 320, 413, 1423, 1867, 2077, 2486
RseI CAYNNNNRTG 2 cut(s) 44, 2217
SaqAI TTAA 7 cut(s) 450, 606, 1173, 1733, 1767, 2099, 2319
SatI GCNGC 5 cut(s) 588, 615, 775, 1128, 1196
Sau96I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
SchI GAGTC 2 cut(s) 654, 1676
ScrFI CCNGG 4 cut(s) 267, 417, 1890, 2298
SduI GDGCHC 2 cut(s) 336, 2296
SfaNI GCATC 8 cut(s) 360, 429, 524, 664, 943, 1112, 1221, 1971
SfcI CTRYAG 3 cut(s) 1999, 2285, 2343
Sfr274I CTCGAG 1 cut(s) 2311
SinI GGWCC 4 cut(s) 527, 563, 1112, 1691
SlaI CTCGAG 1 cut(s) 2311
SmiMI CAYNNNNRTG 2 cut(s) 44, 2217
SmlI CTYRAG 2 cut(s) 1766, 2311
SmoI CTYRAG 2 cut(s) 1766, 2311
SpeI ACTAGT 1 cut(s) 1406
SphI GCATGC 1 cut(s) 702
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 5 cut(s) 626, 774, 1196, 1597, 2142
SspMI CTAG 9 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138, 2165
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 4 cut(s) 265, 415, 1888, 2296
StyI CCWWGG 3 cut(s) 650, 1199, 1687
TaaI ACNGT 7 cut(s) 88, 319, 707, 974, 1066, 1298, 1486
TaiI ACGT 5 cut(s) 240, 1271, 1739, 1859, 2378
TaqI TCGA 9 cut(s) 44, 228, 811, 844, 2223, 2238, 2312, 2519, 2549
TatI WGTACW 2 cut(s) 319, 2485
TauI GCSGC 2 cut(s) 777, 1198
TfiI GAWTC 3 cut(s) 723, 846, 1683
Tru1I TTAA 7 cut(s) 450, 606, 1173, 1733, 1767, 2099, 2319
Tru9I TTAA 7 cut(s) 450, 606, 1173, 1733, 1767, 2099, 2319
TscAI CASTG 6 cut(s) 83, 585, 1252, 1606, 1626, 2294
TseI GCWGC 3 cut(s) 587, 614, 1127
TspGWI ACGGA 7 cut(s) 776, 1734, 1814, 1815, 2018, 2051, 2537
TspRI CASTG 6 cut(s) 83, 585, 1252, 1606, 1626, 2294
Van91I CCANNNNNTGG 1 cut(s) 826
Vha464I CTTAAG 1 cut(s) 1766
VneI GTGCAC 1 cut(s) 2292
VpaK11BI GGWCC 4 cut(s) 527, 563, 1112, 1691
XapI RAATTY 5 cut(s) 379, 1783, 2113, 2227, 2251
XbaI TCTAGA 1 cut(s) 1378
XceI RCATGY 3 cut(s) 702, 886, 1277
XcmI CCANNNNNNNNNTGG 1 cut(s) 2070
XhoI CTCGAG 1 cut(s) 2311
XmnI GAANNNNTTC 1 cut(s) 1748
XspI CTAG 9 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138, 2165
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.