RLG00000011219

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009802
Physical Location & Seq
Reverse (-)
7763458 .. 7764715
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011219

Sequence Viewer

Length: 609 bp
ATGAATTCCTTGGAGTCATTTCTCCATGGAGGAAAATGGCCGACACTTACTCTGCAGTCAGCAGGTCATTTTAATGGACAGTTCTCAGGTTCGGCCTTTGGAACTAGGGAGCAGAGGGAATTTACATTTACAGGACCAGTGAACCTTCGTGCTGGAATAAACAGAATTGCACTCCTTAGCATACCTGTTGGACTACCGAATGTTGGGTTGCATTTTGAGACATGGAAAACAAGAATCCAAGGTCCAGTTTTTCTGGATGGTCTTAGTCGGGGAAGGAAAGATTTAACAATGCAGATGTGGTTCTACAAGGTTGGCCTAAATGGACAGACAATGAATTTGAACTCTCCCAATGGAGCCTCATCCGTTGATTGGATCAGAGCATCACTAGGTCAAGTGTGGATCAACGGGCAGAGTATTGGAAGATACTGGATGGCTTTTGCCAATGGCGACTGTAATATGTGCAGTTACACCCGGACGTACCGGCAGACAAAGTGTCAACTAGGCTGTGGTCAACCAACTCAACGATGGTATCATGTCCCTCGGTCCTGGTTAAAGCCAACAAAAAATTTACTCGTAGTTTTTGAAGAACTTGGTGGTGATCCATCCTAA

Protein Analysis

203

Amino Acids

22.67

Weight (kDa)

9.78

Isoelectric Point (pI)

27.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 118 - 189 6.6e-16 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 53
AclWI GGATC 3 cut(s) 380, 407, 593
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 4 cut(s) 4, 119, 334, 565
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 2 cut(s) 203, 369
AgsI TTSAA 2 cut(s) 340, 584
AhdI GACNNNNNGTC 1 cut(s) 492
AjnI CCWGG 1 cut(s) 545
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 3 cut(s) 380, 407, 593
AoxI GGCC 3 cut(s) 38, 93, 313
ApoI RAATTY 4 cut(s) 4, 119, 334, 565
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AspS9I GGNCC 3 cut(s) 134, 242, 543
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 1 cut(s) 608
AvaII GGWCC 3 cut(s) 134, 242, 543
BaeI ACNNNNGTAYC 2 cut(s) 512, 545
BccI CCATC 3 cut(s) 251, 424, 519
BciT130I CCWGG 1 cut(s) 547
BcnI CCSGG 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 3 cut(s) 105, 386, 500
BfmI CTRYAG 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 53
Bme1390I CCNGG 2 cut(s) 472, 547
Bme18I GGWCC 3 cut(s) 134, 242, 543
BmeRI GACNNNNNGTC 1 cut(s) 492
BmgT120I GGNCC 3 cut(s) 134, 242, 543
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 2 cut(s) 472, 547
BmsI GCATC 1 cut(s) 389
Bpu10I CCTNAGC 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 472
BsaJI CCNNGG 4 cut(s) 9, 25, 238, 539
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 369
Bse118I RCCGGY 1 cut(s) 480
Bse1I ACTGG 3 cut(s) 137, 245, 431
BseBI CCWGG 1 cut(s) 547
BseDI CCNNGG 4 cut(s) 9, 25, 238, 539
BseGI GGATG 4 cut(s) 262, 359, 435, 602
BseLI CCNNNNNNNGG 2 cut(s) 203, 369
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 3 cut(s) 137, 245, 431
BsgI GTGCAG 1 cut(s) 481
BshFI GGCC 3 cut(s) 40, 95, 315
BsiSI CCGG 2 cut(s) 472, 481
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 2 cut(s) 203, 369
BsmAI GTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 3 cut(s) 40, 95, 315
Bsp143I GATC 3 cut(s) 372, 399, 598
Bsp19I CCATGG 1 cut(s) 25
BspANI GGCC 3 cut(s) 40, 95, 315
BspCNI CTCAG 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 355
BspMAI CTGCAG 1 cut(s) 57
BspMI ACCTGC 1 cut(s) 53
BspPI GGATC 3 cut(s) 380, 407, 593
BsrFI RCCGGY 1 cut(s) 480
BsrI ACTGG 3 cut(s) 137, 245, 431
BssAI RCCGGY 1 cut(s) 480
BssECI CCNNGG 4 cut(s) 9, 25, 238, 539
BssMI GATC 3 cut(s) 372, 399, 598
BssT1I CCWWGG 3 cut(s) 9, 25, 238
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 2 cut(s) 81, 452
BstDEI CTNAG 3 cut(s) 85, 176, 263
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 4 cut(s) 262, 359, 435, 602
BstKTI GATC 3 cut(s) 375, 402, 601
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 3 cut(s) 372, 399, 598
BstNI CCWGG 1 cut(s) 547
BstSCI CCNGG 2 cut(s) 470, 545
BstSFI CTRYAG 1 cut(s) 53
BsuRI GGCC 3 cut(s) 40, 95, 315
BtgI CCRYGG 1 cut(s) 25
BtsCI GGATG 4 cut(s) 262, 359, 435, 602
BtsIMutI CAGTG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 480
Cfr13I GGNCC 3 cut(s) 134, 242, 543
Csp6I GTAC 1 cut(s) 478
CviAII CATG 3 cut(s) 26, 222, 533
CviJI RGCY 7 cut(s) 40, 95, 315, 356, 434, 504, 556
CviKI_1 RGCY 7 cut(s) 40, 95, 315, 356, 434, 504, 556
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 3 cut(s) 85, 176, 263
DpnI GATC 3 cut(s) 374, 401, 600
DpnII GATC 3 cut(s) 372, 399, 598
DriI GACNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 38
Eam1105I GACNNNNNGTC 1 cut(s) 492
Eco130I CCWWGG 3 cut(s) 9, 25, 238
Eco47I GGWCC 3 cut(s) 134, 242, 543
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 545
EcoT14I CCWWGG 3 cut(s) 9, 25, 238
ErhI CCWWGG 3 cut(s) 9, 25, 238
FaeI CATG 3 cut(s) 29, 225, 536
FaiI YATR 5 cut(s) 27, 182, 223, 458, 534
FaqI GGGAC 1 cut(s) 521
FatI CATG 3 cut(s) 25, 221, 532
FokI GGATG 4 cut(s) 269, 346, 442, 589
FspBI CTAG 3 cut(s) 105, 386, 500
HaeIII GGCC 3 cut(s) 40, 95, 315
HapII CCGG 2 cut(s) 472, 481
Hin1II CATG 3 cut(s) 29, 225, 536
HincII GTYRAC 2 cut(s) 497, 512
HindII GTYRAC 2 cut(s) 497, 512
HinfI GANTC 2 cut(s) 14, 234
HpaII CCGG 2 cut(s) 472, 481
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 3 cut(s) 142, 497, 512
Hpy188I TCNGA 1 cut(s) 377
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 3 cut(s) 142, 497, 512
HpyAV CCTTC 2 cut(s) 155, 267
HpyCH4III ACNGT 2 cut(s) 81, 452
HpyCH4IV ACGT 1 cut(s) 476
HpyCH4V TGCA 5 cut(s) 55, 170, 211, 292, 462
HpyF3I CTNAG 3 cut(s) 85, 176, 263
HpySE526I ACGT 1 cut(s) 476
Hsp92II CATG 3 cut(s) 29, 225, 536
Kzo9I GATC 3 cut(s) 372, 399, 598
LmnI GCTCC 2 cut(s) 109, 353
LweI GCATC 1 cut(s) 389
MaeI CTAG 3 cut(s) 105, 386, 500
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 464
MalI GATC 3 cut(s) 374, 401, 600
MboI GATC 3 cut(s) 372, 399, 598
MboII GAAGA 2 cut(s) 432, 596
MluCI AATT 5 cut(s) 4, 119, 165, 334, 565
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 4 cut(s) 23, 108, 367, 549
MseI TTAA 3 cut(s) 72, 284, 551
MslI CAYNNNNRTG 1 cut(s) 72
MspI CCGG 2 cut(s) 472, 481
MspR9I CCNGG 2 cut(s) 472, 547
MvaI CCWGG 1 cut(s) 547
NciI CCSGG 1 cut(s) 472
NcoI CCATGG 1 cut(s) 25
NdeII GATC 3 cut(s) 372, 399, 598
NlaIII CATG 3 cut(s) 29, 225, 536
NlaIV GGNNCC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 234
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 355
PspPI GGNCC 3 cut(s) 134, 242, 543
PstI CTGCAG 1 cut(s) 57
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 1 cut(s) 72
SaqAI TTAA 3 cut(s) 72, 284, 551
Sau3AI GATC 3 cut(s) 372, 399, 598
Sau96I GGNCC 3 cut(s) 134, 242, 543
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 472, 547
SetI ASST 8 cut(s) 67, 91, 147, 187, 244, 312, 391, 479
SfaNI GCATC 1 cut(s) 389
SfcI CTRYAG 1 cut(s) 53
SinI GGWCC 3 cut(s) 134, 242, 543
SmiMI CAYNNNNRTG 1 cut(s) 72
Sse9I AATT 5 cut(s) 4, 119, 165, 334, 565
SspMI CTAG 3 cut(s) 105, 386, 500
StyD4I CCNGG 2 cut(s) 470, 545
StyI CCWWGG 3 cut(s) 9, 25, 238
TaaI ACNGT 2 cut(s) 81, 452
TaiI ACGT 1 cut(s) 479
TaqII GACCGA 1 cut(s) 531
TasI AATT 5 cut(s) 4, 119, 165, 334, 565
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 3 cut(s) 72, 284, 551
Tru9I TTAA 3 cut(s) 72, 284, 551
TscAI CASTG 1 cut(s) 144
TspDTI ATGAA 2 cut(s) 17, 347
TspGWI ACGGA 1 cut(s) 352
TspRI CASTG 1 cut(s) 144
VpaK11BI GGWCC 3 cut(s) 134, 242, 543
XapI RAATTY 4 cut(s) 4, 119, 334, 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 522
XspI CTAG 3 cut(s) 105, 386, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.