MD08G1023600.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009802
Physical Location & Seq
Reverse (-)
1693538 .. 1698934
5397 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1023600.v1.1.491

Sequence Viewer

Length: 2559 bp
ATGGAAACTCACTCGGTTTCCAAGCTCTTAGTTTTGTTCTTGACGATGACATTGTTCATAGCTTCCGAGCTGATCCACTGCACCACCGTCACCTACGACAAGAAGGCCATTCTCATCAATGGCCAAAGAAGATTGCTCATTTCTGGATCCATTCACTATCCCAGAAGTACCCCTGAAATGTGGGAAGGCCTCATACAGAAAGCCAAAGATGGAGGCTTGGATGTGATCGATACCTACGTTTTCTGGAACGGCCATGAACCTTCTCCTGGCAACTATTATTTTGAAGGGAGATACGATCTGGTACGGTTCATAAAGACAGTACAGAAAGCAGGGCTCTTTCTTCATCTGCGCATTGGACCTTATGTTTGTGCAGAGTGGAATTTTGGAGGATTTCCTGTTTGGCTGAAATATGTACCTGGCATCAGTTTCAGAACAGATAATGAGCCATTTAAGATGGCAATGCAAGGGTTTACCCAGAAGATTGTCCAGATGATGAAAAATGAGAAGCTATTTGCATCACAAGGTGGTCCTATCATCCTTTCCCAGATTGAGAATGAGTATGGACCGGAGCGGAAGGCACTTGGAGTTGCTGGACAGAACTACATTAACTGGGCTGCTAAAATGGCGGTTGGATTGGATACGGGAGTACCATGGGTGATGTGCAAGGAAGATGATGCCCCGGACCCTATGATAAATGCATGCAACGGTTTTTACTGCGATGGATTCACTCCCAACAAACCTTACAAGCCTACCATGTGGACGGAAGCTTGGAGCGGCTGGTTTACGGAGTTTGGTGGCACAATTCACCATCGACCTGTTCAAGATTTAGCATTCGCAGTTGCTCGATTCATACAAAGGGGCGGCTCATTTGTGAATTACTACATGTATCATGGAGGAACAAACTTCGGACGCACTGCTGGAGGCCCCTTCATTACAACCAGTTACGACTATGATGCCCCCATTGATGAATACGGTTTGATCAGACAACCAAAATACGGTCATCTGAAGGAGCTTCACAAGGCTATTAAATTATGTGAACATTCTTTACTCTCTTCAGAGCCTACCGTCACTTCACTCGGAACCTATCATCAGGCTTATGTATTCAATTCAGGACCGAGAAGCTGCGCAGCGTTTCTCTCAAACTTCCATTCAGTAGAAGCAAGGGTGACTTTTAACAACAAGCACTATGATTTGCCTCCTTGGTCTGTTAGCATCCTTCCTGATTGCAGGAATGAAGTATATAACACTGCAAAGGTGGGAGTTCAAACATCACATGTGCAGATGATCCCAACGAACAGTCGGTTGTTTTCATGGCAGACTTACGATGAAGATATCTCTTCTGTACACGAGAGATCGAGCATACCAGCTGTCGGACTTATTGAGCAGATAAATGTTACTAGAGATACTAGTGACTATCTGTGGTACATGACGAATGTTGACATCAGTTCTTCAGACCTCAGCGGAGGAAAAAAGCCTACTCTTACTGTGCAGTCAGCTGGCCATGCCCTCCATGTCTTCGTCAACGGACAGTTTTCAGGGTCGGCGTTTGGAACTAGGGAGCAGAGGCAATTTACATTTGATGAACCAGTCAACCTTCGCGCTGGAATAAACAGAATTGCACTGCTCAGCATGGCTGTTGGATTACCGAATGTCGGGTTGCATTACGAGTCATGGAAAACAGGAATCCAAGGTCCGGTTTTTCTGGATGGTCTTGGCAACGGAAAGAAAGACTTAACATTGCATAAGTGGTTCAACAAGGTCGGCCTTAAGGGAGAAGCAATGAATTTGGTCTCTCCAAACGGAGCATCATCGGTTGGTTGGATTCGAAGGTCACAAGCTACTCAAACCAAGCAGACGTTGAAATGGTACAAGGCTTATTTCAATGCACCTGGAGGAAACGAGCCGTTGGCGTTGGACATGAGGAGGATGGGAAAGGGACAAGTATGGATCAATGGTCAGAGCATCGGTAGATACTGGATGGCTTATGCCAAAGGCGACTGCAGTTCGTGCAGTTACATTGGGACATTCCGACCCACGAAATGTCAACTTCACTGTGGCCGACCAACTCAACGATGGTATCATGTTCCTCGTTCATGGTTAAAGCCAACGCAAAATTTGGTGGTAGTGTTTGAGGAACTAGGCGGAGATCCATCAAAGATAACCCTAGTGAGGAGATCAGTGACCGGTGTATGTGGTGACTTACATGAGAACCACCCGAACGCTGAAAATTTTGATGTCGAGGGTAATGAAGATTCTAAAACGCTTCATCAGGCTCAGGTTCATCTGCATTGTGCACCAGGGCAGTCTATCTCGAGCATTAAGTTTGCAAGTTTCGGAACTCCTAGCGGAACTTGTGGGAGTTTCCAGCAAGGAACATGTCACGCGACCAACTCACACGCCGTTGTGGAGAAGAATTGTATCGGCCGCGAGAGTTGCTCAGTTGCTGTATCGAATAGCGCCTTCGAGACAGATCCATGTCCGAACGTACTCAAGCGGCTGTCTGTTGAAGCTGTTTGTTCGACAGAAGTTACGACCGATGAAGCCAATTCGAGGACATGA

Protein Analysis

853

Amino Acids

94.89

Weight (kDa)

8.24

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 341 1.6e-122 Glycosyl hydrolases family 35
GHD PF17834 364 - 419 3.2e-22 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 472 - 525 4.3e-09 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 621 - 705 9.2e-27 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 762 - 839 4.6e-23 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 350, 1126
AccBSI CCGCTC 2 cut(s) 571, 774
AccII CGCG 3 cut(s) 1599, 2384, 2427
AciI CCGC 9 cut(s) 571, 626, 774, 861, 1461, 2142, 2346, 2425, 2494
AclWI GGATC 7 cut(s) 67, 141, 154, 1279, 1955, 2141, 2465
AcoI YGGCCR 5 cut(s) 121, 250, 1498, 2056, 2422
AcsI RAATTY 4 cut(s) 379, 1783, 2113, 2227
AcuI CTGAAG 3 cut(s) 1025, 1038, 1434
AdeI CACNNNGTG 1 cut(s) 524
AfaI GTAC 9 cut(s) 169, 303, 321, 414, 648, 1344, 1424, 1868, 2487
AfiI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2169, 2550
AflII CTTAAG 1 cut(s) 1766
AflIII ACRYGT 3 cut(s) 882, 1273, 2375
AgeI ACCGGT 1 cut(s) 2183
AgsI TTSAA 8 cut(s) 284, 821, 1105, 1265, 1753, 1861, 1882, 2507
AhlI ACTAGT 1 cut(s) 1406
AjnI CCWGG 4 cut(s) 265, 415, 1888, 2296
Alw21I GWGCWC 1 cut(s) 2296
Alw26I GTCTC 2 cut(s) 1795, 2459
Alw44I GTGCAC 1 cut(s) 2292
AlwI GGATC 7 cut(s) 67, 141, 154, 1279, 1955, 2141, 2465
AlwNI CAGNNNCTG 1 cut(s) 2444
Ama87I CYCGRG 1 cut(s) 2311
AoxI GGCC 9 cut(s) 105, 121, 187, 250, 922, 1498, 1762, 2056, 2422
ApaLI GTGCAC 1 cut(s) 2292
ApeKI GCWGC 3 cut(s) 614, 1122, 1127
ApoI RAATTY 4 cut(s) 379, 1783, 2113, 2227
ArsI GACNNNNNNTTYG 2 cut(s) 348, 380
AsiGI ACCGGT 1 cut(s) 2183
AspLEI GCGC 4 cut(s) 351, 1127, 1601, 2459
AspS9I GGNCC 7 cut(s) 356, 527, 563, 682, 923, 1112, 1691
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 5 cut(s) 82, 667, 797, 1177, 2207
AsuII TTCGAA 1 cut(s) 1825
AvaI CYCGRG 1 cut(s) 2311
AvaII GGWCC 6 cut(s) 356, 527, 563, 682, 1112, 1691
BaeGI GKGCMC 1 cut(s) 2296
BaeI ACNNNNGTAYC 8 cut(s) 222, 255, 630, 630, 663, 663, 2060, 2093
BalI TGGCCA 2 cut(s) 123, 1500
BamHI GGATCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 336
BarI GAAGNNNNNNTAC 2 cut(s) 177, 209
BauI CACGAG 1 cut(s) 1346
BbsI GAAGAC 1 cut(s) 1507
Bbv12I GWGCWC 1 cut(s) 2296
BbvCI CCTCAGC 1 cut(s) 1457
BbvI GCAGC 3 cut(s) 601, 1109, 1139
BccI CCATC 9 cut(s) 203, 448, 713, 816, 1700, 1921, 1972, 2067, 2158
BceAI ACGGC 3 cut(s) 265, 1888, 2384
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 4 cut(s) 267, 417, 1890, 2298
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 2 cut(s) 1795, 2459
BcuI ACTAGT 1 cut(s) 1406
BfaI CTAG 6 cut(s) 1398, 1407, 1554, 2138, 2165, 2343
BfmI CTRYAG 1 cut(s) 1999
BfoI RGCGCY 1 cut(s) 2460
BfrI CTTAAG 1 cut(s) 1766
BfuI GTATCC 1 cut(s) 631
BisI GCNGC 7 cut(s) 615, 775, 862, 1123, 1128, 2425, 2495
BlpI GCTNAGC 1 cut(s) 1625
BlsI GCNGC 7 cut(s) 616, 776, 863, 1124, 1129, 2426, 2496
Bme1390I CCNGG 5 cut(s) 267, 417, 680, 1890, 2298
Bme18I GGWCC 6 cut(s) 356, 527, 563, 682, 1112, 1691
BmeT110I CYCGRG 1 cut(s) 2311
BmgT120I GGNCC 7 cut(s) 356, 527, 563, 682, 923, 1112, 1691
BmiI GGNNCC 4 cut(s) 148, 684, 925, 1081
BmrFI CCNGG 5 cut(s) 267, 417, 680, 1890, 2298
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 7 cut(s) 429, 524, 664, 943, 1221, 1814, 1971
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 1507
BplI GAGNNNNNCTC 2 cut(s) 2420, 2452
BpmI CTGGAG 2 cut(s) 939, 1911
Bpu10I CCTNAGC 2 cut(s) 1457, 2274
Bpu1102I GCTNAGC 1 cut(s) 1625
Bpu14I TTCGAA 1 cut(s) 1825
BpuEI CTTGAG 1 cut(s) 2474
BpuMI CCSGG 1 cut(s) 680
Bsa29I ATCGAT 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 1795
BsaJI CCNNGG 5 cut(s) 650, 678, 1199, 1687, 2297
BsaWI WCCGGW 3 cut(s) 565, 1693, 2183
Bsc4I CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2169, 2550
Bse118I RCCGGY 1 cut(s) 2183
Bse1I ACTGG 4 cut(s) 614, 939, 1586, 1979
Bse3DI GCAATG 3 cut(s) 465, 1736, 1785
BseBI CCWGG 4 cut(s) 267, 417, 1890, 2298
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 5 cut(s) 650, 678, 1199, 1687, 2297
BseGI GGATG 6 cut(s) 226, 534, 1212, 1711, 1932, 1983
BseLI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2169, 2550
BseMI GCAATG 3 cut(s) 465, 1736, 1785
BseMII CTCAG 4 cut(s) 1471, 1639, 2288, 2451
BseNI ACTGG 4 cut(s) 614, 939, 1586, 1979
BseRI GAGGAG 2 cut(s) 1936, 2185
BseSI GKGCMC 1 cut(s) 2296
BseX3I CGGCCG 1 cut(s) 2422
BseXI GCAGC 3 cut(s) 601, 1109, 1139
BsgI GTGCAG 5 cut(s) 64, 390, 1298, 1508, 2029
Bsh1236I CGCG 3 cut(s) 1599, 2384, 2427
Bsh1285I CGRYCG 2 cut(s) 2425, 2535
BshFI GGCC 9 cut(s) 107, 123, 189, 252, 924, 1500, 1764, 2058, 2424
BshTI ACCGGT 1 cut(s) 2183
BshVI ATCGAT 1 cut(s) 228
BsiEI CGRYCG 2 cut(s) 2425, 2535
BsiHKAI GWGCWC 1 cut(s) 2296
BsiHKCI CYCGRG 1 cut(s) 2311
BsiSI CCGG 4 cut(s) 566, 680, 1694, 2184
BslFI GGGAC 2 cut(s) 1950, 2035
BslI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2169, 2550
BsmAI GTCTC 2 cut(s) 1795, 2459
BsmFI GGGAC 2 cut(s) 1950, 2035
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 9 cut(s) 107, 123, 189, 252, 924, 1500, 1764, 2058, 2424
Bso31I GGTCTC 1 cut(s) 1795
BsoBI CYCGRG 1 cut(s) 2311
Bsp119I TTCGAA 1 cut(s) 1825
Bsp1286I GDGCHC 2 cut(s) 336, 2296
Bsp1407I TGTACA 1 cut(s) 1342
Bsp1720I GCTNAGC 1 cut(s) 1625
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 9 cut(s) 571, 626, 774, 861, 1461, 2142, 2346, 2425, 2494
BspANI GGCC 9 cut(s) 107, 123, 189, 252, 924, 1500, 1764, 2058, 2424
BspCNI CTCAG 4 cut(s) 1470, 1638, 2287, 2450
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 3 cut(s) 1599, 2384, 2427
BspLI GGNNCC 4 cut(s) 148, 684, 925, 1081
BspMAI CTGCAG 1 cut(s) 2003
BspPI GGATC 7 cut(s) 67, 141, 154, 1279, 1955, 2141, 2465
BspT104I TTCGAA 1 cut(s) 1825
BspTI CTTAAG 1 cut(s) 1766
BspTNI GGTCTC 1 cut(s) 1795
BsrBI CCGCTC 2 cut(s) 571, 774
BsrDI GCAATG 3 cut(s) 465, 1736, 1785
BsrFI RCCGGY 1 cut(s) 2183
BsrGI TGTACA 1 cut(s) 1342
BsrI ACTGG 4 cut(s) 614, 939, 1586, 1979
BssAI RCCGGY 1 cut(s) 2183
BssECI CCNNGG 5 cut(s) 650, 678, 1199, 1687, 2297
BssSI CACGAG 1 cut(s) 1346
BssT1I CCWWGG 3 cut(s) 650, 1199, 1687
Bst2BI CACGAG 1 cut(s) 1346
Bst2UI CCWGG 4 cut(s) 267, 417, 1890, 2298
Bst6I CTCTTC 2 cut(s) 1057, 1342
BstAFI CTTAAG 1 cut(s) 1766
BstAPI GCANNNNNTGC 1 cut(s) 2007
BstAUI TGTACA 1 cut(s) 1342
BstBI TTCGAA 1 cut(s) 1825
BstC8I GCNNGC 2 cut(s) 700, 1498
BstDEI CTNAG 5 cut(s) 28, 1457, 1625, 2274, 2437
BstDSI CCRYGG 1 cut(s) 650
BstF5I GGATG 6 cut(s) 226, 534, 1212, 1711, 1932, 1983
BstFNI CGCG 3 cut(s) 1599, 2384, 2427
BstH2I RGCGCY 1 cut(s) 2460
BstHHI GCGC 4 cut(s) 351, 1127, 1601, 2459
BstMAI GTCTC 2 cut(s) 1795, 2459
BstMCI CGRYCG 2 cut(s) 2425, 2535
BstMWI GCNNNNNNNGC 4 cut(s) 623, 1502, 2007, 2433
BstNI CCWGG 4 cut(s) 267, 417, 1890, 2298
BstNSI RCATGY 4 cut(s) 702, 886, 1277, 2379
BstSCI CCNGG 5 cut(s) 265, 415, 678, 1888, 2296
BstSFI CTRYAG 1 cut(s) 1999
BstSLI GKGCMC 1 cut(s) 2296
BstUI CGCG 3 cut(s) 1599, 2384, 2427
BstV1I GCAGC 3 cut(s) 601, 1109, 1139
BstV2I GAAGAC 1 cut(s) 1507
BstX2I RGATCY 3 cut(s) 146, 2146, 2470
BstYI RGATCY 3 cut(s) 146, 2146, 2470
BstZI CGGCCG 1 cut(s) 2422
Bsu15I ATCGAT 1 cut(s) 228
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 9 cut(s) 107, 123, 189, 252, 924, 1500, 1764, 2058, 2424
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 650
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 6 cut(s) 226, 534, 1212, 1711, 1932, 1983
BtsI GCAGTG 4 cut(s) 76, 912, 1245, 1619
BtsIMutI CAGTG 6 cut(s) 76, 912, 1245, 1619, 2050, 2184
Cac8I GCNNGC 2 cut(s) 700, 1498
CaiI CAGNNNCTG 1 cut(s) 2444
CfoI GCGC 4 cut(s) 351, 1127, 1601, 2459
Cfr10I RCCGGY 1 cut(s) 2183
Cfr13I GGNCC 7 cut(s) 356, 527, 563, 682, 923, 1112, 1691
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 918
Csp6I GTAC 9 cut(s) 168, 302, 320, 413, 647, 1343, 1423, 1867, 2486
CspAI ACCGGT 1 cut(s) 2183
CviQI GTAC 9 cut(s) 168, 302, 320, 413, 647, 1343, 1423, 1867, 2486
DdeI CTNAG 5 cut(s) 28, 1457, 1625, 2274, 2437
DraIII CACNNNGTG 1 cut(s) 524
EaeI YGGCCR 5 cut(s) 121, 250, 1498, 2056, 2422
EagI CGGCCG 1 cut(s) 2422
Eam1104I CTCTTC 2 cut(s) 1057, 1342
EarI CTCTTC 2 cut(s) 1057, 1342
EciI GGCGGA 1 cut(s) 2157
EclXI CGGCCG 1 cut(s) 2422
Eco130I CCWWGG 3 cut(s) 650, 1199, 1687
Eco147I AGGCCT 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 1795
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 6 cut(s) 356, 527, 563, 682, 1112, 1691
Eco52I CGGCCG 1 cut(s) 2422
Eco57I CTGAAG 3 cut(s) 1025, 1038, 1434
Eco88I CYCGRG 1 cut(s) 2311
EcoO109I RGGNCCY 1 cut(s) 923
EcoRII CCWGG 4 cut(s) 265, 415, 1888, 2296
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 3 cut(s) 650, 1199, 1687
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 3 cut(s) 650, 1199, 1687
FalI AAGNNNNNCTT 4 cut(s) 1153, 1185, 1715, 1747
FaqI GGGAC 2 cut(s) 1950, 2035
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 7 cut(s) 615, 775, 862, 1123, 1128, 2425, 2495
FokI GGATG 6 cut(s) 233, 521, 1199, 1718, 1939, 1990
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 7 cut(s) 615, 775, 862, 1123, 1128, 2425, 2495
FspBI CTAG 6 cut(s) 1398, 1407, 1554, 2138, 2165, 2343
FspI TGCGCA 2 cut(s) 350, 1126
GlaI GCGC 4 cut(s) 350, 1126, 1600, 2458
GluI GCNGC 7 cut(s) 615, 775, 862, 1123, 1128, 2425, 2495
GsuI CTGGAG 2 cut(s) 939, 1911
HaeII RGCGCY 1 cut(s) 2460
HaeIII GGCC 9 cut(s) 107, 123, 189, 252, 924, 1500, 1764, 2058, 2424
HapII CCGG 4 cut(s) 566, 680, 1694, 2184
HgaI GACGC 1 cut(s) 918
HhaI GCGC 4 cut(s) 351, 1127, 1601, 2459
Hin6I GCGC 4 cut(s) 349, 1125, 1599, 2457
HinP1I GCGC 4 cut(s) 349, 1125, 1599, 2457
HincII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 6 cut(s) 723, 846, 1667, 1683, 1822, 2252
HpaII CCGG 4 cut(s) 566, 680, 1694, 2184
HphI GGTGA 5 cut(s) 82, 667, 797, 1177, 2207
HpyCH4IV ACGT 3 cut(s) 237, 1856, 2484
HpyF10VI GCNNNNNNNGC 4 cut(s) 623, 1502, 2007, 2433
HpyF3I CTNAG 5 cut(s) 28, 1457, 1625, 2274, 2437
HpySE526I ACGT 3 cut(s) 237, 1856, 2484
HspAI GCGC 4 cut(s) 349, 1125, 1599, 2457
Ksp22I TGATCA 1 cut(s) 978
LmnI GCTCC 5 cut(s) 568, 771, 1009, 1558, 1802
Lsp1109I GCAGC 3 cut(s) 601, 1109, 1139
LweI GCATC 7 cut(s) 429, 524, 664, 943, 1221, 1814, 1971
MaeI CTAG 6 cut(s) 1398, 1407, 1554, 2138, 2165, 2343
MaeII ACGT 3 cut(s) 237, 1856, 2484
MbiI CCGCTC 2 cut(s) 571, 774
MflI RGATCY 3 cut(s) 146, 2146, 2470
MhlI GDGCHC 2 cut(s) 336, 2296
MlsI TGGCCA 2 cut(s) 123, 1500
MluNI TGGCCA 2 cut(s) 123, 1500
MlyI GAGTC 1 cut(s) 1676
MmeI TCCRAC 6 cut(s) 610, 1351, 1618, 1799, 1893, 2053
Mox20I TGGCCA 2 cut(s) 123, 1500
Mph1103I ATGCAT 1 cut(s) 700
MscI TGGCCA 2 cut(s) 123, 1500
MseI TTAA 8 cut(s) 450, 606, 1026, 1173, 1733, 1767, 2099, 2319
Msp20I TGGCCA 2 cut(s) 123, 1500
MspA1I CMGCKG 3 cut(s) 1367, 1461, 1496
MspCI CTTAAG 1 cut(s) 1766
MspI CCGG 4 cut(s) 566, 680, 1694, 2184
MspR9I CCNGG 5 cut(s) 267, 417, 680, 1890, 2298
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 4 cut(s) 267, 417, 1890, 2298
MvnI CGCG 3 cut(s) 1599, 2384, 2427
MwoI GCNNNNNNNGC 4 cut(s) 623, 1502, 2007, 2433
NciI CCSGG 1 cut(s) 680
NcoI CCATGG 1 cut(s) 650
NlaIV GGNNCC 4 cut(s) 148, 684, 925, 1081
NmuCI GTSAC 8 cut(s) 88, 1066, 1165, 1409, 1830, 2179, 2195, 2378
NsbI TGCGCA 2 cut(s) 350, 1126
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 4 cut(s) 702, 886, 1277, 2379
NspV TTCGAA 1 cut(s) 1825
PaeI GCATGC 1 cut(s) 702
PaeR7I CTCGAG 1 cut(s) 2311
PceI AGGCCT 1 cut(s) 189
PciI ACATGT 3 cut(s) 882, 1273, 2375
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 830
PfeI GAWTC 5 cut(s) 723, 846, 1683, 1822, 2252
PinAI ACCGGT 1 cut(s) 2183
PkrI GCNGC 7 cut(s) 616, 776, 863, 1124, 1129, 2426, 2496
PleI GAGTC 1 cut(s) 1675
PpsI GAGTC 1 cut(s) 1675
PscI ACATGT 3 cut(s) 882, 1273, 2375
Psp6I CCWGG 4 cut(s) 265, 415, 1888, 2296
PspGI CCWGG 4 cut(s) 265, 415, 1888, 2296
PspN4I GGNNCC 4 cut(s) 148, 684, 925, 1081
PspPI GGNCC 7 cut(s) 356, 527, 563, 682, 923, 1112, 1691
PsrI GAACNNNNNNTAC 2 cut(s) 1029, 1061
PstI CTGCAG 1 cut(s) 2003
PstNI CAGNNNCTG 1 cut(s) 2444
PsuI RGATCY 3 cut(s) 146, 2146, 2470
PvuII CAGCTG 2 cut(s) 1367, 1496
RsaI GTAC 9 cut(s) 169, 303, 321, 414, 648, 1344, 1424, 1868, 2487
RsaNI GTAC 9 cut(s) 168, 302, 320, 413, 647, 1343, 1423, 1867, 2486
SaqAI TTAA 8 cut(s) 450, 606, 1026, 1173, 1733, 1767, 2099, 2319
SatI GCNGC 7 cut(s) 615, 775, 862, 1123, 1128, 2425, 2495
Sau96I GGNCC 7 cut(s) 356, 527, 563, 682, 923, 1112, 1691
SchI GAGTC 1 cut(s) 1676
ScrFI CCNGG 5 cut(s) 267, 417, 680, 1890, 2298
SduI GDGCHC 2 cut(s) 336, 2296
SfaNI GCATC 7 cut(s) 429, 524, 664, 943, 1221, 1814, 1971
SfcI CTRYAG 1 cut(s) 1999
Sfr274I CTCGAG 1 cut(s) 2311
SfuI TTCGAA 1 cut(s) 1825
SinI GGWCC 6 cut(s) 356, 527, 563, 682, 1112, 1691
SlaI CTCGAG 1 cut(s) 2311
SmlI CTYRAG 3 cut(s) 1766, 2311, 2489
SmoI CTYRAG 3 cut(s) 1766, 2311, 2489
SpeI ACTAGT 1 cut(s) 1406
SphI GCATGC 1 cut(s) 702
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 9 cut(s) 571, 626, 774, 861, 1461, 2142, 2346, 2425, 2494
SspMI CTAG 6 cut(s) 1398, 1407, 1554, 2138, 2165, 2343
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 5 cut(s) 265, 415, 678, 1888, 2296
StyI CCWWGG 3 cut(s) 650, 1199, 1687
TaiI ACGT 3 cut(s) 240, 1859, 2487
TaqII GACCGA 2 cut(s) 1129, 2549
TatI WGTACW 2 cut(s) 319, 1342
TauI GCSGC 4 cut(s) 777, 864, 2427, 2497
TfiI GAWTC 5 cut(s) 723, 846, 1683, 1822, 2252
Tru1I TTAA 8 cut(s) 450, 606, 1026, 1173, 1733, 1767, 2099, 2319
Tru9I TTAA 8 cut(s) 450, 606, 1026, 1173, 1733, 1767, 2099, 2319
TscAI CASTG 6 cut(s) 83, 919, 1252, 1626, 2057, 2184
TseFI GTSAC 8 cut(s) 88, 1066, 1165, 1409, 1830, 2179, 2195, 2378
TseI GCWGC 3 cut(s) 614, 1122, 1127
Tsp45I GTSAC 8 cut(s) 88, 1066, 1165, 1409, 1830, 2179, 2195, 2378
TspGWI ACGGA 5 cut(s) 776, 800, 1539, 1734, 1815
TspRI CASTG 6 cut(s) 83, 919, 1252, 1626, 2057, 2184
Vha464I CTTAAG 1 cut(s) 1766
VneI GTGCAC 1 cut(s) 2292
VpaK11BI GGWCC 6 cut(s) 356, 527, 563, 682, 1112, 1691
XapI RAATTY 4 cut(s) 379, 1783, 2113, 2227
XceI RCATGY 4 cut(s) 702, 886, 1277, 2379
XcmI CCANNNNNNNNNTGG 1 cut(s) 2070
XhoI CTCGAG 1 cut(s) 2311
XspI CTAG 6 cut(s) 1398, 1407, 1554, 2138, 2165, 2343
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.