pycom15g01740

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009802
Physical Location & Seq
Reverse (-)
1000747 .. 1005380
4634 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g01740.5

Sequence Viewer

Length: 2559 bp
ATGGAAACTCGCTCGGTTTCCAAGCTCTTAGTCTTGTTCATGTCGATGGCATTGTTCTTGGGTTCTGAGCTCATCCACTGCACCACCGTCACCTACGACAAGAAGGCCATTCTCATCGATGGCCAGAGAAGATTGCTCATTTCTGGATCCATTCATTATCCCAGAAGCACCCCTGAAATGTGGGAAGGCCTCATACAGAAAGCAAAAGATGGAGGCTTGGATGTTATCGACACCTACGTTTTCTGGAACGGACATGAACCTTCTCCTGGCAATTATTATTTTGAGGGGAGATACGATCTAGTAGGGTTCATAAAGACAGTAGAGAAAGCAGGGCTCCTTCTTCATCTGCGCATTGGGCCTTATGTTTGTGCAGAGTGGAATTTTGGAGGATTTCCTGTTTGGCTGAAATATGTACCCGGCATCAGTTTCAGGACGGATAATGGGCCATTTAAGATGGCAATGCAAGGGTTCACCCAGAAGATTGTCCAGATGATGAAAGATGAGAAGCTATTTGCATCACAAGGTGGTCCCATCATCCTTTCCCAGATTGAGAATGAGTATGGACCGGAGAGCAAGGCATTGGGAGCTGCTGGCCATAACTACATTAACTGGGCTGCTCAAATGGCGGTTGGATTGGATACCGGAGTCCCATGGGTAATGTGCAAGGAAGACGATGCCCCAGACCCTATGATAAATGCATGCAACGGTTTTTACTGTGATGGATTCACTCCCAACAAACCTTACAAGCCTGCCATGTGGACGGAAGCTTGGAGCGGCTGGTTTACAGAATTTGGTGGCACAATTCACCATCGACCTGTCCAAGATTTAGCATTTGCAGTTGGTCGATTCATACAGAAGGGTGGCTCATATGTGAATTACTACATGTATCATGGAGGAACAAACTTCGGACGAACAGCTGGAGGCCCCTTCATTACAACCAGTTACGACTATGATGCTCCCATTGATGAATATGGTTTGATCAGAGAACCTAAATATGGTCATCTGAAGGAGCTTCACAAGGCTATCAAGCTATGTGAACATTCTTTACTCTCTTCAGAGCCTACAGTCACTTCACTAGGAGCCTATCATCAGGCTTATGTATTCAATTCAGGACCAAAAAGATGCGCAGCGTTTCTCTCAAACTTCCATTCAACAGGAGCAAGAGTGACTTTCAATAACTTGCACTATGATTTGCCTCCTTGGTCTGTTAGCATCCTTCCTGATTGCAGGAATGAAGTATTCAACACCGCAAAGGTGGGAGTTCAAACGTCACACGTGCAGATGATCCCGACGAACAGTCCGTTGTTTTCATGGCAGACTTACGATGAAGATATCTCTTCTCTAAACGATGGGTCAAGCATACCGGCTGTTGGACTTCTAGAGCAGATAAATGTTACTAGAGATACTAGTGACTATCTGTGGTACATGACGAATGTTGACATCAGTTCATCAGACCTCGGCGGAGGAAAAAAGCCTACTCTTACTGTGCAGTCAGCTGGTCATGCCCTCCATGTCTTCGTCAACGGACAGTATTCAGGTTCGGCGTTTGGAACTAGGGAGCAGAGGCAATTCACATTTGCTGAACCAGTCAACCTCCGTGCTGGAATAAACAGAATTGCACTGCTCAGCATAGCTGTTGGATTACCAAATGTTGGGTTGCATTATGAGTCATGGAAAACAGGAATCCAAGGTCCGGTTTTTCTGGATGGTCTTGGCCACGGAAAGAAAGACTTAACGTCGCAGAAGTGGTTCTACAAGGTCGGCCTTAAGGGAGAAGAAATGAATTTAGTCTCTCCAAACGGAGCCTCATCAGTTGATTGGATCCGGGGGTCACTAGCTACCCAAGCCAAGCAGATGTTGAAATGGTACAAGGCTTATTTCAATGCACCCGGAGGAAATGAGCCATTGGCGTTGGACATGAGTAGCATGGGAAAGGGACAAGTATGGATCGATCGGCAGAGCATCGGTAGATACTGGATGGCTTATTCCAAAGGTGACTGCAGTTCGTGCAGTTACATTGGGACATTCCGTCCCACGAAATGTCAACTTGGCTGTGGCCAACCAACTCAACAATGGTATCATGTTCCTCGTTCATGGTTAAAGCCGACGCAAAATTTGGTGGTAGTGTTTGAGGAACTAGGCGGAGATCCATCGAAGATTACCCTTGTGAAAAGATCAGTGACCGGTGTTTGTGGTGACTTACATGAGAACCACCCAAATGTCGAAAATTTTGATGTTGAGGGCAATGAAAATTCTAAAACGCTTCACCAAGCACAGGTTCATCTGCAGTGTGCACCAGGGCAGTCTATCTCGAGCATTAAGTTTGCAAGTTTCGGAACTCCTACTGGAACTTGTGGGAGTTTCCAGCAAGGAACGTGTCATGCGACAAACTCACACGCCGTGGTGGAGAAGAATTGTATCAGCCGCGAGAGTTGCTCGGTTGCTGTAACCAATAGCATTTTCAAGACAGATCCATGTCCGAATGTACTCAAACGGCTATCAGTTGAAGCTGTTTGTTCGACGGAAATTACAACCAATCAACCCAATTCGAGGAGATGA

Protein Analysis

853

Amino Acids

94.24

Weight (kDa)

7.11

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 350, 1126
AccB7I CCANNNNNTGG 1 cut(s) 1652
AccBSI CCGCTC 1 cut(s) 774
AccII CGCG 1 cut(s) 2427
AciI CCGC 6 cut(s) 626, 774, 1248, 1461, 2142, 2425
AclWI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
AcoI YGGCCR 4 cut(s) 121, 592, 1714, 2056
AcsI RAATTY 6 cut(s) 379, 788, 1783, 2113, 2227, 2251
AcuI CTGAAG 2 cut(s) 1025, 1038
AcvI CACGTG 1 cut(s) 1276
AdeI CACNNNGTG 2 cut(s) 524, 2401
AfaI GTAC 4 cut(s) 414, 1424, 1868, 2487
AfiI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
AflII CTTAAG 1 cut(s) 1766
AflIII ACRYGT 3 cut(s) 882, 1273, 2375
AgeI ACCGGT 1 cut(s) 2183
AgsI TTSAA 9 cut(s) 1105, 1152, 1174, 1243, 1265, 1861, 1882, 2464, 2507
AhdI GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
AhlI ACTAGT 1 cut(s) 1406
AjnI CCWGG 2 cut(s) 265, 2296
AjuI GAANNNNNNNTTGG 4 cut(s) 2262, 2294, 2444, 2476
Alw21I GWGCWC 2 cut(s) 72, 2296
Alw26I GTCTC 1 cut(s) 1795
Alw44I GTGCAC 1 cut(s) 2292
AlwI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
Ama87I CYCGRG 1 cut(s) 2311
ApaLI GTGCAC 1 cut(s) 2292
ApeKI GCWGC 3 cut(s) 587, 614, 1127
ApoI RAATTY 6 cut(s) 379, 788, 1783, 2113, 2227, 2251
ArsI GACNNNNNNTTYG 2 cut(s) 2098, 2130
AsiGI ACCGGT 1 cut(s) 2183
Asp700I GAANNNNTTC 1 cut(s) 1748
AspLEI GCGC 2 cut(s) 351, 1127
AspS9I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
AsuC2I CCSGG 3 cut(s) 417, 1826, 1890
AsuHPI GGTGA 6 cut(s) 82, 463, 797, 2006, 2207, 2258
AvaI CYCGRG 1 cut(s) 2311
AvaII GGWCC 4 cut(s) 527, 563, 1112, 1691
BaeGI GKGCMC 1 cut(s) 2296
BaeI ACNNNNGTAYC 4 cut(s) 630, 663, 2060, 2093
BalI TGGCCA 4 cut(s) 123, 594, 1716, 2058
BamHI GGATCC 2 cut(s) 146, 1821
BanII GRGCYC 2 cut(s) 72, 336
BarI GAAGNNNNNNTAC 4 cut(s) 177, 209, 1736, 1768
BbrPI CACGTG 1 cut(s) 1276
BbsI GAAGAC 2 cut(s) 675, 1507
Bbv12I GWGCWC 2 cut(s) 72, 2296
BbvI GCAGC 3 cut(s) 574, 601, 1139
BceAI ACGGC 2 cut(s) 2384, 2510
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 2 cut(s) 267, 2298
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 3 cut(s) 417, 1826, 1890
BcoDI GTCTC 1 cut(s) 1795
BcuI ACTAGT 1 cut(s) 1406
BfaI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
BfmI CTRYAG 3 cut(s) 1062, 1999, 2285
BfrI CTTAAG 1 cut(s) 1766
BfuI GTATCC 1 cut(s) 631
BisI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
BlpI GCTNAGC 1 cut(s) 1625
BlsI GCNGC 5 cut(s) 589, 616, 776, 1129, 2426
Bme1390I CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
Bme18I GGWCC 4 cut(s) 527, 563, 1112, 1691
BmeRI GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
BmeT110I CYCGRG 1 cut(s) 2311
BmgT120I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
BmiI GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
BmrFI CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 7 cut(s) 429, 524, 664, 943, 1112, 1221, 1971
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 2 cut(s) 675, 1507
BplI GAGNNNNNCTC 2 cut(s) 2420, 2452
BpmI CTGGAG 1 cut(s) 939
Bpu1102I GCTNAGC 1 cut(s) 1625
BpuMI CCSGG 3 cut(s) 417, 1826, 1890
Bsa29I ATCGAT 2 cut(s) 117, 1950
BsaAI YACGTR 1 cut(s) 1276
BsaJI CCNNGG 8 cut(s) 650, 1199, 1456, 1687, 1717, 1825, 2297, 2400
BsaWI WCCGGW 4 cut(s) 565, 641, 1693, 2183
BsaXI ACNNNNNCTCC 2 cut(s) 1149, 1179
Bsc4I CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
Bse118I RCCGGY 2 cut(s) 1363, 2183
Bse1I ACTGG 5 cut(s) 614, 939, 1586, 1979, 2350
Bse3DI GCAATG 2 cut(s) 465, 2251
BseBI CCWGG 2 cut(s) 267, 2298
BseCI ATCGAT 2 cut(s) 117, 1950
BseDI CCNNGG 8 cut(s) 650, 1199, 1456, 1687, 1717, 1825, 2297, 2400
BseGI GGATG 6 cut(s) 72, 226, 534, 1212, 1711, 1983
BseLI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
BseMI GCAATG 2 cut(s) 465, 2251
BseMII CTCAG 2 cut(s) 57, 1639
BseNI ACTGG 5 cut(s) 614, 939, 1586, 1979, 2350
BseSI GKGCMC 1 cut(s) 2296
BseXI GCAGC 3 cut(s) 574, 601, 1139
BsgI GTGCAG 5 cut(s) 64, 390, 1298, 1508, 2029
Bsh1236I CGCG 1 cut(s) 2427
Bsh1285I CGRYCG 1 cut(s) 1954
BshTI ACCGGT 1 cut(s) 2183
BshVI ATCGAT 2 cut(s) 117, 1950
BsiEI CGRYCG 1 cut(s) 1954
BsiHKAI GWGCWC 2 cut(s) 72, 2296
BsiHKCI CYCGRG 1 cut(s) 2311
BsiSI CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
BslFI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
BslI CCNNNNNNNGG 7 cut(s) 266, 995, 1370, 1652, 1693, 2275, 2550
BsmAI GTCTC 1 cut(s) 1795
BsmFI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
BsoBI CYCGRG 1 cut(s) 2311
Bsp1286I GDGCHC 3 cut(s) 72, 336, 2296
Bsp1720I GCTNAGC 1 cut(s) 1625
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 6 cut(s) 626, 774, 1248, 1461, 2142, 2425
BspCNI CTCAG 2 cut(s) 58, 1638
BspDI ATCGAT 2 cut(s) 117, 1950
BspFNI CGCG 1 cut(s) 2427
BspLI GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
BspMAI CTGCAG 2 cut(s) 2003, 2289
BspPI GGATC 8 cut(s) 141, 154, 1279, 1816, 1829, 1955, 2141, 2465
BspTI CTTAAG 1 cut(s) 1766
BsrBI CCGCTC 1 cut(s) 774
BsrDI GCAATG 2 cut(s) 465, 2251
BsrFI RCCGGY 2 cut(s) 1363, 2183
BsrI ACTGG 5 cut(s) 614, 939, 1586, 1979, 2350
BssAI RCCGGY 2 cut(s) 1363, 2183
BssECI CCNNGG 8 cut(s) 650, 1199, 1456, 1687, 1717, 1825, 2297, 2400
BssT1I CCWWGG 3 cut(s) 650, 1199, 1687
Bst2UI CCWGG 2 cut(s) 267, 2298
Bst4CI ACNGT 8 cut(s) 88, 319, 707, 716, 1066, 1298, 1486, 1530
Bst6I CTCTTC 2 cut(s) 1057, 1342
BstAFI CTTAAG 1 cut(s) 1766
BstAPI GCANNNNNTGC 1 cut(s) 2007
BstBAI YACGTR 1 cut(s) 1276
BstC8I GCNNGC 3 cut(s) 592, 700, 750
BstDEI CTNAG 3 cut(s) 28, 66, 1625
BstDSI CCRYGG 3 cut(s) 650, 1717, 2400
BstF5I GGATG 6 cut(s) 72, 226, 534, 1212, 1711, 1983
BstFNI CGCG 1 cut(s) 2427
BstHHI GCGC 2 cut(s) 351, 1127
BstMAI GTCTC 1 cut(s) 1795
BstMCI CGRYCG 1 cut(s) 1954
BstMWI GCNNNNNNNGC 7 cut(s) 355, 584, 623, 1502, 1844, 2007, 2433
BstNI CCWGG 2 cut(s) 267, 2298
BstNSI RCATGY 2 cut(s) 702, 886
BstSCI CCNGG 5 cut(s) 265, 415, 1824, 1888, 2296
BstSFI CTRYAG 3 cut(s) 1062, 1999, 2285
BstSLI GKGCMC 1 cut(s) 2296
BstUI CGCG 1 cut(s) 2427
BstV1I GCAGC 3 cut(s) 574, 601, 1139
BstV2I GAAGAC 2 cut(s) 675, 1507
BstX2I RGATCY 4 cut(s) 146, 1821, 2146, 2470
BstYI RGATCY 4 cut(s) 146, 1821, 2146, 2470
Bsu15I ATCGAT 2 cut(s) 117, 1950
BsuI GTATCC 1 cut(s) 631
BsuTUI ATCGAT 2 cut(s) 117, 1950
BtgI CCRYGG 3 cut(s) 650, 1717, 2400
BtsCI GGATG 6 cut(s) 72, 226, 534, 1212, 1711, 1983
BtsI GCAGTG 3 cut(s) 76, 1619, 2294
BtsIMutI CAGTG 4 cut(s) 76, 1619, 2184, 2294
Cac8I GCNNGC 3 cut(s) 592, 700, 750
CfoI GCGC 2 cut(s) 351, 1127
Cfr10I RCCGGY 2 cut(s) 1363, 2183
Cfr13I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
ClaI ATCGAT 2 cut(s) 117, 1950
CseI GACGC 1 cut(s) 2116
Csp6I GTAC 4 cut(s) 413, 1423, 1867, 2486
CspAI ACCGGT 1 cut(s) 2183
CviQI GTAC 4 cut(s) 413, 1423, 1867, 2486
DdeI CTNAG 3 cut(s) 28, 66, 1625
DraIII CACNNNGTG 2 cut(s) 524, 2401
DriI GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
EaeI YGGCCR 4 cut(s) 121, 592, 1714, 2056
Eam1104I CTCTTC 2 cut(s) 1057, 1342
Eam1105I GACNNNNNGTC 3 cut(s) 1296, 1735, 2028
EarI CTCTTC 2 cut(s) 1057, 1342
EciI GGCGGA 2 cut(s) 1476, 2157
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 3 cut(s) 650, 1199, 1687
Eco147I AGGCCT 1 cut(s) 189
Eco24I GRGCYC 2 cut(s) 72, 336
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 4 cut(s) 527, 563, 1112, 1691
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 2 cut(s) 1025, 1038
Eco72I CACGTG 1 cut(s) 1276
Eco88I CYCGRG 1 cut(s) 2311
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 923
EcoRII CCWGG 2 cut(s) 265, 2296
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 3 cut(s) 650, 1199, 1687
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 2 cut(s) 72, 336
ErhI CCWWGG 3 cut(s) 650, 1199, 1687
FalI AAGNNNNNCTT 4 cut(s) 1153, 1185, 1715, 1747
FaqI GGGAC 5 cut(s) 513, 632, 1950, 2016, 2035
FauNDI CATATG 1 cut(s) 868
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
FokI GGATG 6 cut(s) 59, 233, 521, 1199, 1718, 1990
FriOI GRGCYC 2 cut(s) 72, 336
Fsp4HI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
FspBI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
FspI TGCGCA 2 cut(s) 350, 1126
GlaI GCGC 2 cut(s) 350, 1126
GluI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
GsuI CTGGAG 1 cut(s) 939
HapII CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
HgaI GACGC 1 cut(s) 2116
HhaI GCGC 2 cut(s) 351, 1127
Hin6I GCGC 2 cut(s) 349, 1125
HinP1I GCGC 2 cut(s) 349, 1125
HincII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindII GTYRAC 4 cut(s) 1438, 1522, 1591, 2045
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 5 cut(s) 645, 723, 846, 1667, 1683
HpaII CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
HphI GGTGA 6 cut(s) 82, 463, 797, 2006, 2207, 2258
Hpy166II GTNNAC 9 cut(s) 471, 759, 783, 1037, 1438, 1522, 1591, 2045, 2294
Hpy188I TCNGA 8 cut(s) 67, 908, 983, 1005, 1057, 1453, 2336, 2481
Hpy8I GTNNAC 9 cut(s) 471, 759, 783, 1037, 1438, 1522, 1591, 2045, 2294
Hpy99I CGWCG 4 cut(s) 1294, 1741, 2110, 2524
HpyAV CCTTC 8 cut(s) 97, 179, 270, 347, 850, 937, 1000, 1226
HpyCH4III ACNGT 8 cut(s) 88, 319, 707, 716, 1066, 1298, 1486, 1530
HpyCH4IV ACGT 5 cut(s) 237, 1268, 1275, 1736, 2375
HpyF10VI GCNNNNNNNGC 7 cut(s) 355, 584, 623, 1502, 1844, 2007, 2433
HpyF3I CTNAG 3 cut(s) 28, 66, 1625
HpySE526I ACGT 5 cut(s) 237, 1268, 1275, 1736, 2375
HspAI GCGC 2 cut(s) 349, 1125
Ksp22I TGATCA 1 cut(s) 978
LmnI GCTCC 9 cut(s) 339, 584, 771, 961, 1009, 1079, 1157, 1558, 1802
Lsp1109I GCAGC 3 cut(s) 574, 601, 1139
LweI GCATC 7 cut(s) 429, 524, 664, 943, 1112, 1221, 1971
MaeI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
MaeII ACGT 5 cut(s) 237, 1268, 1275, 1736, 2375
MbiI CCGCTC 1 cut(s) 774
MflI RGATCY 4 cut(s) 146, 1821, 2146, 2470
MhlI GDGCHC 3 cut(s) 72, 336, 2296
MlsI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MluNI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MlyI GAGTC 2 cut(s) 654, 1676
MmeI TCCRAC 4 cut(s) 610, 1351, 1618, 1893
Mox20I TGGCCA 4 cut(s) 123, 594, 1716, 2058
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 1748
MscI TGGCCA 4 cut(s) 123, 594, 1716, 2058
MseI TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
MslI CAYNNNNRTG 2 cut(s) 44, 2217
Msp20I TGGCCA 4 cut(s) 123, 594, 1716, 2058
MspA1I CMGCKG 2 cut(s) 917, 1496
MspCI CTTAAG 1 cut(s) 1766
MspI CCGG 8 cut(s) 417, 566, 642, 1364, 1694, 1825, 1890, 2184
MspR9I CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
MvaI CCWGG 2 cut(s) 267, 2298
MvnI CGCG 1 cut(s) 2427
MwoI GCNNNNNNNGC 7 cut(s) 355, 584, 623, 1502, 1844, 2007, 2433
NciI CCSGG 3 cut(s) 417, 1826, 1890
NcoI CCATGG 1 cut(s) 650
NdeI CATATG 1 cut(s) 868
NlaIV GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
NmeAIII GCCGAG 1 cut(s) 1437
NmuCI GTSAC 9 cut(s) 88, 1066, 1165, 1269, 1409, 1830, 1994, 2179, 2195
NsbI TGCGCA 2 cut(s) 350, 1126
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 2 cut(s) 702, 886
PaeI GCATGC 1 cut(s) 702
PaeR7I CTCGAG 1 cut(s) 2311
PceI AGGCCT 1 cut(s) 189
PciI ACATGT 1 cut(s) 882
PcsI WCGNNNNNNNCGW 3 cut(s) 234, 1298, 2381
PdmI GAANNNNTTC 1 cut(s) 1748
PfeI GAWTC 3 cut(s) 723, 846, 1683
PflMI CCANNNNNTGG 1 cut(s) 1652
PinAI ACCGGT 1 cut(s) 2183
PkrI GCNGC 5 cut(s) 589, 616, 776, 1129, 2426
Ple19I CGATCG 1 cut(s) 1954
PleI GAGTC 2 cut(s) 653, 1675
PmaCI CACGTG 1 cut(s) 1276
PmlI CACGTG 1 cut(s) 1276
PpsI GAGTC 2 cut(s) 653, 1675
Ppu21I YACGTR 1 cut(s) 1276
PscI ACATGT 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 265, 2296
PspCI CACGTG 1 cut(s) 1276
PspGI CCWGG 2 cut(s) 265, 2296
PspN4I GGNNCC 7 cut(s) 148, 335, 529, 925, 1081, 1804, 1823
PspPI GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
PsrI GAACNNNNNNTAC 2 cut(s) 1029, 1061
PstI CTGCAG 2 cut(s) 2003, 2289
PsuI RGATCY 4 cut(s) 146, 1821, 2146, 2470
PvuI CGATCG 1 cut(s) 1954
PvuII CAGCTG 2 cut(s) 917, 1496
RsaI GTAC 4 cut(s) 414, 1424, 1868, 2487
RsaNI GTAC 4 cut(s) 413, 1423, 1867, 2486
RseI CAYNNNNRTG 2 cut(s) 44, 2217
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
SatI GCNGC 5 cut(s) 588, 615, 775, 1128, 2425
Sau96I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1691
SchI GAGTC 2 cut(s) 654, 1676
ScrFI CCNGG 5 cut(s) 267, 417, 1826, 1890, 2298
SduI GDGCHC 3 cut(s) 72, 336, 2296
SfaNI GCATC 7 cut(s) 429, 524, 664, 943, 1112, 1221, 1971
SfcI CTRYAG 3 cut(s) 1062, 1999, 2285
Sfr274I CTCGAG 1 cut(s) 2311
SinI GGWCC 4 cut(s) 527, 563, 1112, 1691
SlaI CTCGAG 1 cut(s) 2311
SmiMI CAYNNNNRTG 2 cut(s) 44, 2217
SmlI CTYRAG 2 cut(s) 1766, 2311
SmoI CTYRAG 2 cut(s) 1766, 2311
SpeI ACTAGT 1 cut(s) 1406
SphI GCATGC 1 cut(s) 702
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 6 cut(s) 626, 774, 1248, 1461, 2142, 2425
SspMI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
SstI GAGCTC 1 cut(s) 72
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 5 cut(s) 265, 415, 1824, 1888, 2296
StyI CCWWGG 3 cut(s) 650, 1199, 1687
TaaI ACNGT 8 cut(s) 88, 319, 707, 716, 1066, 1298, 1486, 1530
TaiI ACGT 5 cut(s) 240, 1271, 1278, 1739, 2378
TatI WGTACW 1 cut(s) 2485
TauI GCSGC 2 cut(s) 777, 2427
TfiI GAWTC 3 cut(s) 723, 846, 1683
Tru1I TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
Tru9I TTAA 6 cut(s) 450, 606, 1733, 1767, 2099, 2319
TscAI CASTG 4 cut(s) 83, 1626, 2184, 2294
TseFI GTSAC 9 cut(s) 88, 1066, 1165, 1269, 1409, 1830, 1994, 2179, 2195
TseI GCWGC 3 cut(s) 587, 614, 1127
Tsp45I GTSAC 9 cut(s) 88, 1066, 1165, 1269, 1409, 1830, 1994, 2179, 2195
TspRI CASTG 4 cut(s) 83, 1626, 2184, 2294
Van91I CCANNNNNTGG 1 cut(s) 1652
Vha464I CTTAAG 1 cut(s) 1766
VneI GTGCAC 1 cut(s) 2292
VpaK11BI GGWCC 4 cut(s) 527, 563, 1112, 1691
XapI RAATTY 6 cut(s) 379, 788, 1783, 2113, 2227, 2251
XbaI TCTAGA 1 cut(s) 1378
XceI RCATGY 2 cut(s) 702, 886
XcmI CCANNNNNNNNNTGG 1 cut(s) 2070
XhoI CTCGAG 1 cut(s) 2311
XmnI GAANNNNTTC 1 cut(s) 1748
XspI CTAG 8 cut(s) 299, 1076, 1379, 1398, 1407, 1554, 1835, 2138
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.