Rh6BG428900

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009802
Physical Location & Seq
Forward (+)
64929377 .. 64936163
6787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG428900.1

Sequence Viewer

Length: 1704 bp
ATGGAAACTCACTCGGTTTCCAAGCTCTTAGCTTTGTACTTGGTGGCCGCCTTGTTTGTGGGTTCTGAGCTGATCCAATGCACCACTGTGAGCTATGACAAGAAGGCCATTCTCATCAATGGCCGGAGAAGACTACTCATCTCCGGCTCAATTCACTATCCCAGAAGCACCCCTGAAATGTGGGAAGGTCTTATACAGAAAGCTAAAGATGGAGGGTTGGATGTGATTGACACTTATGTTTTCTGGAATGGTCATGAACCTTCTCCTGGCAATTACTATTTTGAGGGGAGATATGATCTTGTACGGTTCATAAGGACAGTACAGAAAGCAGGGCTCTACCTTCATCTGCGAATTGGACCTTATGTTTGTGCAGAATGGAATTTTGGAGGATTTCCTGTTTGGCTAAAATATGTTCCTGGAATCAGCTTCAGAACAGACAATGGGCCATTCAAGATGGCAATGCAAGGGTTTACCCAGAAAATTGTCCAGATGATGAAAAATGAAAGGCTATTTGCATCACAAGGTGGCCCCATAATCCTTTCTCAGATTGAGAATGAATATGGGCCAGAAAGCAAGGCACTTGGAGCTGCTGGTCATAACTACATAAACTGGGCTGCCAAAATGGCCGTTGGAATGAATACCGGAGTCCCATGGGTGATGTGCAAGGAAGATGATGCCCCCGACCCTATGATAAATGCATGTAATGGTTTTTACTGTGATGGTTTCACTCCCAACAAACCTTACAAGCCTACTTTGTGGACTGAGGCTTGGAGTGGCTGGTTTTCTGAGTTTGGCGGCACAGTTCACCACCGACCTGTTCAAGATTTGGCATTTGCAGTCGCTCGCTTCATACAGAAGGGTGGCTCATATGTTAATTACTACATGTATCATGGAGGTACCAACTTCGGACGCACTGCTGGAGGCCCATTCATTACAACAAGCTATGACTATGATGCTCCAATTGATGAATATGGTTTGATCAGAGAACCTAAATATACTCATCTGAAGGAGCTTCATAGGGCTATTAAGCTATCTGAACATGCTTTAGTTTCTTCTGATCCTACTGTTACTTCATTAGGAACCTATCAGCAGGCTTATGTATTCAATTCAGGACCAAGAAGCTGTGCAGCTTTTCTCTCGAACTTTCATATGAAATCTGCTGCAACTGTGACTTTTAATAACATGCACTATGATCTACCTGCTTGGTCCGTCAGCATCCTTCCTGATTGCAGAAATGCAGTATTTAACACTGCAAAGGTGGGAGTCCCAACTTCACGGGTGCAGATGACCCCAACCCACAATCGTTTGCTTTATTGGGAGACTTATGGTGAAGATCTTTCCTCTCTACATGATAGGTCGAGCATATCAACTATTGGACTTCTAGAGCAGATAAATGTCACTAGAGATACCAGTGACTATCTGTGGTACATGACCAACATTGAGATCAATTCATGGGAGTCTTTTCTCCATGGAGGAAAATGGCCGACACTTACTCTGCAGTCAGCAGGTCATGCCGTCCATGTCTTCATCAATGGACAGTTCTCAGGTTCAGCCTTTGGAACTAGGGAGCAGAGGGAATTTACATTTACAAAACCAGTGAACCTTCATGCTGGAATAAACAGAATTGCACTGCTCAGCATAGCTGTTGGACTACCGGTTAGTGCCCTTCCTACCTCAACTTTCAGATGGGGCAGCATGAGCTGA

Protein Analysis

567

Amino Acids

63.52

Weight (kDa)

8.04

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 341 5.4e-122 Glycosyl hydrolases family 35
GHD PF17834 364 - 420 7.1e-23 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 473 - 540 1.7e-08 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1207, 1496
Acc65I GGTACC 1 cut(s) 896
AccB1I GGYRCC 1 cut(s) 896
AciI CCGC 2 cut(s) 48, 795
AclWI GGATC 2 cut(s) 67, 1052
AcoI YGGCCR 4 cut(s) 45, 121, 624, 1481
AcsI RAATTY 2 cut(s) 379, 1577
AcuI CTGAAG 2 cut(s) 412, 1025
AdeI CACNNNGTG 1 cut(s) 524
AfaI GTAC 5 cut(s) 38, 303, 321, 898, 1427
AfiI CCNNNNNNNGG 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 882
AgeI ACCGGT 1 cut(s) 1654
AgsI TTSAA 3 cut(s) 451, 821, 1105
AjnI CCWGG 2 cut(s) 265, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AleI CACNNNNGTG 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 1313
AlwI GGATC 2 cut(s) 67, 1052
AoxI GGCC 9 cut(s) 45, 105, 121, 443, 526, 563, 624, 922, 1481
ApeKI GCWGC 5 cut(s) 587, 614, 1127, 1160, 1692
ApoI RAATTY 2 cut(s) 379, 1577
ArsI GACNNNNNNTTYG 2 cut(s) 348, 380
AsiGI ACCGGT 1 cut(s) 1654
Asp718I GGTACC 1 cut(s) 896
AspS9I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1206
AsuHPI GGTGA 3 cut(s) 667, 797, 1340
AvaII GGWCC 3 cut(s) 356, 1112, 1206
BaeGI GKGCMC 1 cut(s) 1666
BanI GGYRCC 1 cut(s) 896
BanII GRGCYC 1 cut(s) 336
BarI GAAGNNNNNNTAC 2 cut(s) 177, 209
BbsI GAAGAC 2 cut(s) 136, 1516
BbvI GCAGC 4 cut(s) 574, 601, 1139, 1147
BccI CCATC 4 cut(s) 203, 448, 713, 1680
BceAI ACGGC 2 cut(s) 611, 1499
BciT130I CCWGG 2 cut(s) 267, 417
BclI TGATCA 1 cut(s) 978
BcoDI GTCTC 1 cut(s) 1313
BfaI CTAG 3 cut(s) 1382, 1401, 1563
BfmI CTRYAG 1 cut(s) 1496
BfuAI ACCTGC 2 cut(s) 1207, 1496
BglI GCCNNNNNGGC 1 cut(s) 623
BglII AGATCT 1 cut(s) 1333
BisI GCNGC 7 cut(s) 48, 588, 615, 796, 1128, 1161, 1693
BlpI GCTNAGC 1 cut(s) 1634
BlsI GCNGC 7 cut(s) 49, 589, 616, 797, 1129, 1162, 1694
Bme1390I CCNGG 2 cut(s) 267, 417
Bme18I GGWCC 3 cut(s) 356, 1112, 1206
BmgT120I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1206
BmiI GGNNCC 3 cut(s) 529, 898, 1081
BmrFI CCNGG 2 cut(s) 267, 417
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 4 cut(s) 524, 664, 943, 1224
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 2 cut(s) 136, 1516
BpmI CTGGAG 1 cut(s) 939
Bpu1102I GCTNAGC 1 cut(s) 1634
BsaJI CCNNGG 2 cut(s) 650, 1468
BsaWI WCCGGW 2 cut(s) 641, 1654
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse118I RCCGGY 1 cut(s) 1654
Bse1I ACTGG 3 cut(s) 614, 1410, 1595
Bse3DI GCAATG 1 cut(s) 465
BseBI CCWGG 2 cut(s) 267, 417
BseDI CCNNGG 2 cut(s) 650, 1468
BseGI GGATG 2 cut(s) 226, 1215
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 6 cut(s) 57, 557, 753, 777, 1557, 1648
BseNI ACTGG 3 cut(s) 614, 1410, 1595
BseSI GKGCMC 1 cut(s) 1666
BseXI GCAGC 4 cut(s) 574, 601, 1139, 1147
BsgI GTGCAG 3 cut(s) 390, 1146, 1301
BshFI GGCC 9 cut(s) 47, 107, 123, 445, 528, 565, 626, 924, 1483
BshNI GGYRCC 1 cut(s) 896
BshTI ACCGGT 1 cut(s) 1654
BsiSI CCGG 4 cut(s) 124, 144, 642, 1655
BslFI GGGAC 2 cut(s) 632, 1250
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 1 cut(s) 1313
BsmFI GGGAC 2 cut(s) 632, 1250
BsnI GGCC 9 cut(s) 47, 107, 123, 445, 528, 565, 626, 924, 1483
Bsp1286I GDGCHC 2 cut(s) 336, 1666
Bsp143I GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
Bsp1720I GCTNAGC 1 cut(s) 1634
Bsp19I CCATGG 2 cut(s) 650, 1468
BspACI CCGC 2 cut(s) 48, 795
BspANI GGCC 9 cut(s) 47, 107, 123, 445, 528, 565, 626, 924, 1483
BspCNI CTCAG 6 cut(s) 58, 556, 754, 778, 1556, 1647
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 529, 898, 1081
BspMAI CTGCAG 1 cut(s) 1500
BspMI ACCTGC 2 cut(s) 1207, 1496
BspPI GGATC 2 cut(s) 67, 1052
BspT107I GGYRCC 1 cut(s) 896
BsrDI GCAATG 1 cut(s) 465
BsrFI RCCGGY 1 cut(s) 1654
BsrI ACTGG 3 cut(s) 614, 1410, 1595
BssAI RCCGGY 1 cut(s) 1654
BssECI CCNNGG 2 cut(s) 650, 1468
BssMI GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
BssT1I CCWWGG 2 cut(s) 650, 1468
Bst2UI CCWGG 2 cut(s) 267, 417
Bst4CI ACNGT 8 cut(s) 88, 306, 319, 716, 802, 1066, 1168, 1539
BstAPI GCANNNNNTGC 1 cut(s) 1511
BstC8I GCNNGC 2 cut(s) 844, 1092
BstDEI CTNAG 7 cut(s) 28, 66, 543, 762, 786, 1543, 1634
BstDSI CCRYGG 2 cut(s) 650, 1468
BstF5I GGATG 2 cut(s) 226, 1215
BstKTI GATC 7 cut(s) 75, 298, 981, 1060, 1195, 1336, 1446
BstMAI GTCTC 1 cut(s) 1313
BstMBI GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
BstMWI GCNNNNNNNGC 4 cut(s) 584, 623, 1511, 1698
BstNI CCWGG 2 cut(s) 267, 417
BstNSI RCATGY 4 cut(s) 702, 886, 1043, 1186
BstSCI CCNGG 2 cut(s) 265, 415
BstSFI CTRYAG 1 cut(s) 1496
BstSLI GKGCMC 1 cut(s) 1666
BstV1I GCAGC 4 cut(s) 574, 601, 1139, 1147
BstV2I GAAGAC 2 cut(s) 136, 1516
BstX2I RGATCY 1 cut(s) 1333
BstYI RGATCY 1 cut(s) 1333
BsuRI GGCC 9 cut(s) 47, 107, 123, 445, 528, 565, 626, 924, 1483
BtgI CCRYGG 2 cut(s) 650, 1468
BtsCI GGATG 2 cut(s) 226, 1215
BtsI GCAGTG 3 cut(s) 912, 1248, 1628
BtsIMutI CAGTG 6 cut(s) 84, 912, 1248, 1417, 1602, 1628
BveI ACCTGC 2 cut(s) 1207, 1496
Cac8I GCNNGC 2 cut(s) 844, 1092
CciI TCATGA 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 1654
Cfr13I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1206
CseI GACGC 1 cut(s) 918
Csp6I GTAC 5 cut(s) 37, 302, 320, 897, 1426
CspAI ACCGGT 1 cut(s) 1654
CviQI GTAC 5 cut(s) 37, 302, 320, 897, 1426
DdeI CTNAG 7 cut(s) 28, 66, 543, 762, 786, 1543, 1634
DpnI GATC 7 cut(s) 74, 297, 980, 1059, 1194, 1335, 1445
DpnII GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
DraIII CACNNNGTG 1 cut(s) 524
EaeI YGGCCR 4 cut(s) 45, 121, 624, 1481
Eco130I CCWWGG 2 cut(s) 650, 1468
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 3 cut(s) 356, 1112, 1206
Eco57I CTGAAG 2 cut(s) 412, 1025
EcoRII CCWGG 2 cut(s) 265, 415
EcoT14I CCWWGG 2 cut(s) 650, 1468
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 2 cut(s) 650, 1468
FaqI GGGAC 2 cut(s) 632, 1250
FauNDI CATATG 2 cut(s) 868, 1149
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 7 cut(s) 48, 588, 615, 796, 1128, 1161, 1693
FokI GGATG 2 cut(s) 233, 1202
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 7 cut(s) 48, 588, 615, 796, 1128, 1161, 1693
FspBI CTAG 3 cut(s) 1382, 1401, 1563
GluI GCNGC 7 cut(s) 48, 588, 615, 796, 1128, 1161, 1693
GsuI CTGGAG 1 cut(s) 939
HaeIII GGCC 9 cut(s) 47, 107, 123, 445, 528, 565, 626, 924, 1483
HapII CCGG 4 cut(s) 124, 144, 642, 1655
HgaI GACGC 1 cut(s) 918
HinfI GANTC 4 cut(s) 420, 645, 1263, 1457
HpaII CCGG 4 cut(s) 124, 144, 642, 1655
HphI GGTGA 3 cut(s) 667, 797, 1340
Hpy166II GTNNAC 4 cut(s) 471, 759, 805, 1600
Hpy188III TCNNGA 9 cut(s) 244, 254, 451, 487, 821, 1110, 1138, 1223, 1382
Hpy8I GTNNAC 4 cut(s) 471, 759, 805, 1600
HpyAV CCTTC 9 cut(s) 97, 179, 270, 350, 850, 1000, 1229, 1613, 1676
HpyCH4III ACNGT 8 cut(s) 88, 306, 319, 716, 802, 1066, 1168, 1539
HpyF10VI GCNNNNNNNGC 4 cut(s) 584, 623, 1511, 1698
HpyF3I CTNAG 7 cut(s) 28, 66, 543, 762, 786, 1543, 1634
KpnI GGTACC 1 cut(s) 900
Ksp22I TGATCA 1 cut(s) 978
Kzo9I GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
LmnI GCTCC 4 cut(s) 584, 961, 1009, 1567
Lsp1109I GCAGC 4 cut(s) 574, 601, 1139, 1147
LweI GCATC 4 cut(s) 524, 664, 943, 1224
MaeI CTAG 3 cut(s) 1382, 1401, 1563
MaeIII GTNAC 4 cut(s) 1066, 1168, 1396, 1412
MalI GATC 7 cut(s) 74, 297, 980, 1059, 1194, 1335, 1445
MboI GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
MboII GAAGA 5 cut(s) 141, 680, 1044, 1343, 1516
MfeI CAATTG 1 cut(s) 960
MflI RGATCY 1 cut(s) 1333
MhlI GDGCHC 2 cut(s) 336, 1666
MlyI GAGTC 3 cut(s) 654, 1272, 1466
MmeI TCCRAC 3 cut(s) 198, 610, 1627
Mph1103I ATGCAT 1 cut(s) 700
MseI TTAA 4 cut(s) 873, 1026, 1176, 1245
MslI CAYNNNNRTG 1 cut(s) 86
MspI CCGG 4 cut(s) 124, 144, 642, 1655
MspR9I CCNGG 2 cut(s) 267, 417
MunI CAATTG 1 cut(s) 960
MvaI CCWGG 2 cut(s) 267, 417
MwoI GCNNNNNNNGC 4 cut(s) 584, 623, 1511, 1698
NcoI CCATGG 2 cut(s) 650, 1468
NdeI CATATG 2 cut(s) 868, 1149
NdeII GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
NlaIV GGNNCC 3 cut(s) 529, 898, 1081
NmuCI GTSAC 3 cut(s) 1168, 1396, 1412
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 4 cut(s) 702, 886, 1043, 1186
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 1 cut(s) 253
PciI ACATGT 1 cut(s) 882
PfeI GAWTC 1 cut(s) 420
PfoI TCCNGGA 1 cut(s) 415
PinAI ACCGGT 1 cut(s) 1654
PkrI GCNGC 7 cut(s) 49, 589, 616, 797, 1129, 1162, 1694
PleI GAGTC 3 cut(s) 653, 1271, 1465
PpsI GAGTC 3 cut(s) 653, 1271, 1465
PscI ACATGT 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 265, 415
PspGI CCWGG 2 cut(s) 265, 415
PspN4I GGNNCC 3 cut(s) 529, 898, 1081
PspPI GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1206
PstI CTGCAG 1 cut(s) 1500
PsuI RGATCY 1 cut(s) 1333
RsaI GTAC 5 cut(s) 38, 303, 321, 898, 1427
RsaNI GTAC 5 cut(s) 37, 302, 320, 897, 1426
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 4 cut(s) 873, 1026, 1176, 1245
SatI GCNGC 7 cut(s) 48, 588, 615, 796, 1128, 1161, 1693
Sau3AI GATC 7 cut(s) 72, 295, 978, 1057, 1192, 1333, 1443
Sau96I GGNCC 7 cut(s) 356, 443, 527, 563, 923, 1112, 1206
SchI GAGTC 3 cut(s) 654, 1272, 1466
ScrFI CCNGG 2 cut(s) 267, 417
SduI GDGCHC 2 cut(s) 336, 1666
SfaNI GCATC 4 cut(s) 524, 664, 943, 1224
SfcI CTRYAG 1 cut(s) 1496
SinI GGWCC 3 cut(s) 356, 1112, 1206
SmiMI CAYNNNNRTG 1 cut(s) 86
SsiI CCGC 2 cut(s) 48, 795
SspMI CTAG 3 cut(s) 1382, 1401, 1563
StyD4I CCNGG 2 cut(s) 265, 415
StyI CCWWGG 2 cut(s) 650, 1468
TaaI ACNGT 8 cut(s) 88, 306, 319, 716, 802, 1066, 1168, 1539
TaqI TCGA 2 cut(s) 1139, 1358
TatI WGTACW 2 cut(s) 36, 319
TauI GCSGC 2 cut(s) 50, 798
TfiI GAWTC 1 cut(s) 420
Tru1I TTAA 4 cut(s) 873, 1026, 1176, 1245
Tru9I TTAA 4 cut(s) 873, 1026, 1176, 1245
TscAI CASTG 6 cut(s) 91, 919, 1255, 1417, 1602, 1635
TseFI GTSAC 3 cut(s) 1168, 1396, 1412
TseI GCWGC 5 cut(s) 587, 614, 1127, 1160, 1692
Tsp45I GTSAC 3 cut(s) 1168, 1396, 1412
TspGWI ACGGA 1 cut(s) 1198
TspRI CASTG 6 cut(s) 91, 919, 1255, 1417, 1602, 1635
VpaK11BI GGWCC 3 cut(s) 356, 1112, 1206
XapI RAATTY 2 cut(s) 379, 1577
XbaI TCTAGA 1 cut(s) 1381
XceI RCATGY 4 cut(s) 702, 886, 1043, 1186
XspI CTAG 3 cut(s) 1382, 1401, 1563
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.