AT1G49970

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014451
Physical Location & Seq
Reverse (-)
18501570 .. 18504654
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49970.1

Sequence Viewer

Length: 1164 bp
ATGGCGACTGCTCTGGTTTCTCCTCTTACTTCACAACTCAACCATGAAGCTGTCTGTTCCAAGTTTGTGTTACCCAAATCGCCGTTCATGTCGGGATCTAAGCTCTTTTCATCGAACATGCCTTGCTCTACTGTTCCTAGACGCACAAGAAGATCACACTGCTTTGCTTCTGCTAAAGATATGAGCTTTGACCATATCCCTAAACAATTTCGTGGAGACAATCTCAAAGACGGAGTGATGCAGAACTTCAAGAACGTACCACAATATTTTTACGGGCTTAATTCAGCTCAAATGGATATGTTCATGACAGAAGATAGCCCTGTCCGTAGGCAAGCGGAAAAAGTTACAGAGGAAAGCATCTCGTCTAGAAATAACTATTTGAACAATGGAGGAATATGGAGTATGTCCGGTATGAATGCAGCAGATGCTAGAAGATATAGCATGAGTGTCCAGATGTACAGAGGTGGAGGTGGCGGCGGAGGATCTGAAAGACCAAGAACTGCTCCTCCAGATTTGCCTTCTTTGCTATTGGATGCTCGCATATGCTACCTTGGCATGCCAATCGTACCTGCAGTTACTGAGTTACTCGTTGCTCAATTTATGTGGTTAGACTATGATAACCCAACAAAGCCCATCTACTTATATATAAATTCACCCGGGACCCAGAATGAGAAGATGGAAACTGTTGGATCAGAAACAGAGGCTTATGCCATAGCCGATACTATTTCTTATTGCAAATCAGATGTATACACTATCAATTGTGGCATGGCTTTTGGTCAAGCAGCAATGCTTCTTTCTCTCGGGAAAAAAGGTTATCGTGCCGTACAACCACATTCATCAACAAAATTATATCTGCCAAAAGTAAACAGATCGAGTGGAGCTGCCATAGACATGTGGATAAAGGCCAAGGAACTTGATGCAAATACCGAGTACTACATCGAGCTGTTAGCTAAGGGAACAGGGAAATCCAAAGAGCAGATCAACGAGGACATCAAGCGACCTAAATATCTCCAAGCTCAAGCAGCCATTGACTATGGAATTGCAGACAAGATAGCTGACTCGCAGGACAGTTCTTTCGAGAAACGGGATTACGATGGGACCCTTGCCCAGAGAGCTATGAGACCTGGAGGAGGCAGTCCAGCAGCTCCAGCCGGACTAAGATGA

Protein Analysis

387

Amino Acids

42.63

Weight (kDa)

8.82

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 173 - 354 3.6e-37 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014451)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49970
fragaria_vesca FvH4_2g04150
malus_domestica MD05G1098400.v1.1 MD10G1104200.v1.1
prunus_persica Prupe.8G143200_v2.0.a1
pyrus_communis pycom05g09630 pycom10g08980
rosa_chinensis RchiOBHm_Chr6g0253511
rosa_laevigata RLG00000015013
rosa_multiflora Rmu_ssc0000079.1_g000005
rosa_roxburghii Rroxscaffold_7G00211820
rosa_rugosa Rorug05G0544700 Rorug05G0544700
rosa_samantha Rh6AG059800 Rh6BG053800 Rh6DG051100
rosa_wichuraiana Rw6G005320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccI GTMKAC 1 cut(s) 747
AciI CCGC 3 cut(s) 335, 474, 477
AclWI GGATC 3 cut(s) 103, 490, 697
AcsI RAATTY 1 cut(s) 649
AfaI GTAC 5 cut(s) 258, 458, 567, 825, 932
AfiI CCNNNNNNNGG 1 cut(s) 1130
AflIII ACRYGT 1 cut(s) 891
AgsI TTSAA 2 cut(s) 250, 382
AjnI CCWGG 1 cut(s) 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AloI GAACNNNNNNTCC 2 cut(s) 490, 522
Alw26I GTCTC 2 cut(s) 210, 1114
AlwI GGATC 3 cut(s) 103, 490, 697
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 2 cut(s) 656, 800
AoxI GGCC 1 cut(s) 903
ApeKI GCWGC 5 cut(s) 419, 782, 881, 1022, 1142
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
AspS9I GGNCC 2 cut(s) 660, 1098
AsuC2I CCSGG 2 cut(s) 657, 658
AsuHPI GGTGA 1 cut(s) 645
AvaI CYCGRG 2 cut(s) 656, 800
AvaII GGWCC 2 cut(s) 660, 1098
BbvI GCAGC 5 cut(s) 431, 794, 868, 1034, 1154
BccI CCATC 3 cut(s) 641, 670, 1088
BceAI ACGGC 2 cut(s) 67, 806
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 1 cut(s) 1125
BcnI CCSGG 2 cut(s) 657, 658
BcoDI GTCTC 2 cut(s) 210, 1114
BfaI CTAG 3 cut(s) 138, 366, 429
BfmI CTRYAG 1 cut(s) 570
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 6 cut(s) 420, 475, 783, 882, 1023, 1143
BlsI GCNGC 6 cut(s) 421, 476, 784, 883, 1024, 1144
BmcAI AGTACT 1 cut(s) 932
Bme1390I CCNGG 3 cut(s) 657, 658, 1125
Bme18I GGWCC 2 cut(s) 660, 1098
BmeT110I CYCGRG 2 cut(s) 656, 800
BmgT120I GGNCC 2 cut(s) 660, 1098
BmiI GGNNCC 4 cut(s) 661, 662, 1099, 1100
BmrFI CCNGG 3 cut(s) 657, 658, 1125
BmsI GCATC 5 cut(s) 228, 366, 415, 523, 907
BplI GAGNNNNNCTC 2 cut(s) 207, 239
BpmI CTGGAG 3 cut(s) 492, 1131, 1146
Bpu10I CCTNAGC 1 cut(s) 951
BpuEI CTTGAG 1 cut(s) 1002
BpuMI CCSGG 2 cut(s) 657, 658
BsaI GGTCTC 1 cut(s) 1114
BsaJI CCNNGG 3 cut(s) 550, 656, 906
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 2 cut(s) 490, 520
Bsc4I CCNNNNNNNGG 1 cut(s) 1130
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 1 cut(s) 1125
BseDI CCNNGG 3 cut(s) 550, 656, 906
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 1 cut(s) 1130
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 1 cut(s) 570
BseRI GAGGAG 3 cut(s) 12, 495, 1143
BseXI GCAGC 5 cut(s) 431, 794, 868, 1034, 1154
BshFI GGCC 1 cut(s) 905
BsiHKCI CYCGRG 2 cut(s) 656, 800
BsiSI CCGG 3 cut(s) 408, 657, 1152
BslFI GGGAC 2 cut(s) 673, 1111
BslI CCNNNNNNNGG 1 cut(s) 1130
BsmAI GTCTC 2 cut(s) 210, 1114
BsmFI GGGAC 2 cut(s) 673, 1111
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 1 cut(s) 905
Bso31I GGTCTC 1 cut(s) 1114
BsoBI CYCGRG 2 cut(s) 656, 800
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 6 cut(s) 95, 152, 482, 689, 869, 978
BspACI CCGC 3 cut(s) 335, 474, 477
BspANI GGCC 1 cut(s) 905
BspCNI CTCAG 1 cut(s) 571
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 4 cut(s) 661, 662, 1099, 1100
BspMAI CTGCAG 1 cut(s) 574
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 3 cut(s) 103, 490, 697
BspTNI GGTCTC 1 cut(s) 1114
BsrDI GCAATG 1 cut(s) 792
BsrGI TGTACA 1 cut(s) 456
BssECI CCNNGG 3 cut(s) 550, 656, 906
BssMI GATC 6 cut(s) 95, 152, 482, 689, 869, 978
BssNAI GTATAC 1 cut(s) 748
BssT1I CCWWGG 2 cut(s) 550, 906
Bst1107I GTATAC 1 cut(s) 748
Bst2UI CCWGG 1 cut(s) 1125
Bst4CI ACNGT 3 cut(s) 133, 685, 1070
BstAPI GCANNNNNTGC 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 3 cut(s) 333, 538, 557
BstDEI CTNAG 4 cut(s) 99, 579, 951, 1157
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 6 cut(s) 98, 155, 485, 692, 872, 981
BstMAI GTCTC 2 cut(s) 210, 1114
BstMBI GATC 6 cut(s) 95, 152, 482, 689, 869, 978
BstMWI GCNNNNNNNGC 6 cut(s) 425, 523, 552, 1022, 1112, 1148
BstNI CCWGG 1 cut(s) 1125
BstNSI RCATGY 3 cut(s) 121, 559, 895
BstSCI CCNGG 3 cut(s) 655, 656, 1123
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 5 cut(s) 431, 794, 868, 1034, 1154
BstX2I RGATCY 2 cut(s) 95, 482
BstYI RGATCY 2 cut(s) 95, 482
BstZ17I GTATAC 1 cut(s) 748
BsuRI GGCC 1 cut(s) 905
BtsCI GGATG 1 cut(s) 538
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 157
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 3 cut(s) 333, 538, 557
CaiI CAGNNNCTG 1 cut(s) 578
CciI TCATGA 1 cut(s) 303
Cfr13I GGNCC 2 cut(s) 660, 1098
Cfr9I CCCGGG 1 cut(s) 656
CseI GACGC 1 cut(s) 150
Csp6I GTAC 5 cut(s) 257, 457, 566, 824, 931
CspCI CAANNNNNGTGG 2 cut(s) 584, 619
CviAII CATG 8 cut(s) 44, 88, 118, 304, 442, 556, 766, 892
CviQI GTAC 5 cut(s) 257, 457, 566, 824, 931
DdeI CTNAG 4 cut(s) 99, 579, 951, 1157
DpnI GATC 6 cut(s) 97, 154, 484, 691, 871, 980
DpnII GATC 6 cut(s) 95, 152, 482, 689, 869, 978
EciI GGCGGA 1 cut(s) 492
Eco130I CCWWGG 2 cut(s) 550, 906
Eco31I GGTCTC 1 cut(s) 1114
Eco47I GGWCC 2 cut(s) 660, 1098
Eco88I CYCGRG 2 cut(s) 656, 800
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 2 cut(s) 660, 1098
EcoRII CCWGG 1 cut(s) 1123
EcoT14I CCWWGG 2 cut(s) 550, 906
ErhI CCWWGG 2 cut(s) 550, 906
FaeI CATG 8 cut(s) 47, 91, 121, 307, 445, 559, 769, 895
FaqI GGGAC 2 cut(s) 673, 1111
FatI CATG 8 cut(s) 43, 87, 117, 303, 441, 555, 765, 891
FauNDI CATATG 1 cut(s) 542
FblI GTMKAC 1 cut(s) 747
Fnu4HI GCNGC 6 cut(s) 420, 475, 783, 882, 1023, 1143
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 6 cut(s) 420, 475, 783, 882, 1023, 1143
FspBI CTAG 3 cut(s) 138, 366, 429
GluI GCNGC 6 cut(s) 420, 475, 783, 882, 1023, 1143
GsuI CTGGAG 3 cut(s) 492, 1131, 1146
HaeIII GGCC 1 cut(s) 905
HapII CCGG 3 cut(s) 408, 657, 1152
HgaI GACGC 1 cut(s) 150
Hin1II CATG 8 cut(s) 47, 91, 121, 307, 445, 559, 769, 895
HinfI GANTC 1 cut(s) 1058
HpaII CCGG 3 cut(s) 408, 657, 1152
HphI GGTGA 1 cut(s) 645
Hpy166II GTNNAC 2 cut(s) 748, 865
Hpy188I TCNGA 3 cut(s) 487, 694, 742
Hpy188III TCNNGA 8 cut(s) 93, 250, 304, 366, 451, 509, 802, 1078
Hpy8I GTNNAC 2 cut(s) 748, 865
HpyAV CCTTC 1 cut(s) 528
HpyCH4III ACNGT 3 cut(s) 133, 685, 1070
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 6 cut(s) 241, 419, 572, 735, 920, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 425, 523, 552, 1022, 1112, 1148
HpyF3I CTNAG 4 cut(s) 99, 579, 951, 1157
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 8 cut(s) 47, 91, 121, 307, 445, 559, 769, 895
KflI GGGWCCC 2 cut(s) 660, 1098
Kzo9I GATC 6 cut(s) 95, 152, 482, 689, 869, 978
LmnI GCTCC 3 cut(s) 508, 878, 1150
Lsp1109I GCAGC 5 cut(s) 431, 794, 868, 1034, 1154
LweI GCATC 5 cut(s) 228, 366, 415, 523, 907
MaeI CTAG 3 cut(s) 138, 366, 429
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 4 cut(s) 69, 343, 574, 582
MalI GATC 6 cut(s) 97, 154, 484, 691, 871, 980
MboI GATC 6 cut(s) 95, 152, 482, 689, 869, 978
MboII GAAGA 4 cut(s) 162, 323, 444, 685
MfeI CAATTG 1 cut(s) 757
MflI RGATCY 2 cut(s) 95, 482
MluCI AATT 7 cut(s) 206, 280, 596, 649, 757, 845, 1038
MlyI GAGTC 1 cut(s) 1052
MmeI TCCRAC 1 cut(s) 667
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 890
MspI CCGG 3 cut(s) 408, 657, 1152
MspR9I CCNGG 3 cut(s) 657, 658, 1125
MunI CAATTG 1 cut(s) 757
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 1 cut(s) 1125
MwoI GCNNNNNNNGC 6 cut(s) 425, 523, 552, 1022, 1112, 1148
NciI CCSGG 2 cut(s) 657, 658
NdeI CATATG 1 cut(s) 542
NdeII GATC 6 cut(s) 95, 152, 482, 689, 869, 978
NlaIII CATG 8 cut(s) 47, 91, 121, 307, 445, 559, 769, 895
NlaIV GGNNCC 4 cut(s) 661, 662, 1099, 1100
NspI RCATGY 3 cut(s) 121, 559, 895
PaeI GCATGC 1 cut(s) 559
PagI TCATGA 1 cut(s) 303
PciI ACATGT 1 cut(s) 891
PctI GAATGC 1 cut(s) 421
PkrI GCNGC 6 cut(s) 421, 476, 784, 883, 1024, 1144
PleI GAGTC 1 cut(s) 1052
PpsI GAGTC 1 cut(s) 1052
PpuMI RGGWCCY 2 cut(s) 660, 1098
PscI ACATGT 1 cut(s) 891
Psp5II RGGWCCY 2 cut(s) 660, 1098
Psp6I CCWGG 1 cut(s) 1123
PspGI CCWGG 1 cut(s) 1123
PspN4I GGNNCC 4 cut(s) 661, 662, 1099, 1100
PspPI GGNCC 2 cut(s) 660, 1098
PspPPI RGGWCCY 2 cut(s) 660, 1098
PstI CTGCAG 1 cut(s) 574
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 2 cut(s) 95, 482
RsaI GTAC 5 cut(s) 258, 458, 567, 825, 932
RsaNI GTAC 5 cut(s) 257, 457, 566, 824, 931
RseI CAYNNNNRTG 1 cut(s) 890
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 6 cut(s) 420, 475, 783, 882, 1023, 1143
Sau3AI GATC 6 cut(s) 95, 152, 482, 689, 869, 978
Sau96I GGNCC 2 cut(s) 660, 1098
ScaI AGTACT 1 cut(s) 932
SchI GAGTC 1 cut(s) 1052
ScrFI CCNGG 3 cut(s) 657, 658, 1125
SfaNI GCATC 5 cut(s) 228, 366, 415, 523, 907
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 2 cut(s) 660, 1098
SmaI CCCGGG 1 cut(s) 658
SmiMI CAYNNNNRTG 1 cut(s) 890
SmlI CTYRAG 1 cut(s) 1017
SmoI CTYRAG 1 cut(s) 1017
SphI GCATGC 1 cut(s) 559
Sse9I AATT 7 cut(s) 206, 280, 596, 649, 757, 845, 1038
SsiI CCGC 3 cut(s) 335, 474, 477
SspI AATATT 1 cut(s) 266
SspMI CTAG 3 cut(s) 138, 366, 429
StyD4I CCNGG 3 cut(s) 655, 656, 1123
StyI CCWWGG 2 cut(s) 550, 906
TaaI ACNGT 3 cut(s) 133, 685, 1070
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 113, 872, 939, 1077
TasI AATT 7 cut(s) 206, 280, 596, 649, 757, 845, 1038
TatI WGTACW 2 cut(s) 456, 930
TauI GCSGC 1 cut(s) 477
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 164
TseI GCWGC 5 cut(s) 419, 782, 881, 1022, 1142
TspDTI ATGAA 6 cut(s) 60, 76, 99, 292, 428, 825
TspGWI ACGGA 2 cut(s) 246, 314
TspMI CCCGGG 1 cut(s) 656
TspRI CASTG 1 cut(s) 164
VpaK11BI GGWCC 2 cut(s) 660, 1098
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 1 cut(s) 649
XbaI TCTAGA 1 cut(s) 365
XceI RCATGY 3 cut(s) 121, 559, 895
XmaI CCCGGG 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 747
XspI CTAG 3 cut(s) 138, 366, 429
ZrmI AGTACT 1 cut(s) 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.