AT1G50650

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0017921
Physical Location & Seq
Reverse (-)
18763640 .. 18764367
728 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50650.1

Sequence Viewer

Length: 525 bp
ATGATGAGCATTAAGCTGACATTGTGTGCCTTAATCTTCTTCCTCTTAAATTCACTTCTTCATCATGTTCTCGGTCACGATAACCAACTTAACACAACCTCGTCATGGCTTAAGAGCCACATCAAAGCAGCTACAACAACAAATTGGGGTAGACCAAAGCCACCGATGTGTAAACCATGGATTTGCAAACGGTCCGGACCATCAGAGGCTCGAATGAGATGTTGTCGTAACCAATGCGTTGATGTCTTGTCCGATCCAAACCATTGTCGGTTCTGTTTCAAGAGTTGTAGGTTCGCATTGTCGTGCTGCGATGGAGATTGTGTGGACACGAACACTGATCCGTCTAATTGTGGACAATGCGGGAACGAGTGTGAATCCGGTGCGCCTTGTGAGTTTGGTATGTGTGGTTATGCTGCTCCATCGTCACAGCCTGGAAAACGTCATCGTCGTCACAAGTTTCATCGGCCACGTCCTCCTCCTTCTCCGGACAGTAAACTTAATTATGATGATCATGATGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

174

Amino Acids

19.53

Weight (kDa)

8.45

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 58 - 138 1.8e-24 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 151
AccIII TCCGGA 2 cut(s) 194, 484
AciI CCGC 1 cut(s) 360
AclWI GGATC 2 cut(s) 248, 332
AcoI YGGCCR 1 cut(s) 464
AcsI RAATTY 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 105
AflII CTTAAG 1 cut(s) 110
AgsI TTSAA 1 cut(s) 280
AjiI CACGTC 1 cut(s) 470
AjnI CCWGG 1 cut(s) 430
AleI CACNNNNGTG 1 cut(s) 166
AluBI AGCT 2 cut(s) 16, 131
AluI AGCT 2 cut(s) 16, 131
AlwI GGATC 2 cut(s) 248, 332
Aor13HI TCCGGA 2 cut(s) 194, 484
AoxI GGCC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 128, 306, 413
ApoI RAATTY 1 cut(s) 49
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 192, 197
AvaII GGWCC 2 cut(s) 192, 197
BbvI GCAGC 3 cut(s) 140, 293, 400
BccI CCATC 3 cut(s) 208, 305, 427
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 1 cut(s) 508
BfrI CTTAAG 1 cut(s) 110
BisI GCNGC 3 cut(s) 129, 307, 414
BlsI GCNGC 3 cut(s) 130, 308, 415
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 2 cut(s) 192, 197
BmgBI CACGTC 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 192, 197
BmrFI CCNGG 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 176
BsaWI WCCGGW 3 cut(s) 194, 377, 484
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 105
BseAI TCCGGA 2 cut(s) 194, 484
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 176
BseLI CCNNNNNNNGG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 465
BseXI GCAGC 3 cut(s) 140, 293, 400
BshFI GGCC 1 cut(s) 466
BsiSI CCGG 3 cut(s) 195, 378, 485
BslI CCNNNNNNNGG 1 cut(s) 105
BsnI GGCC 1 cut(s) 466
Bsp13I TCCGGA 2 cut(s) 194, 484
Bsp143I GATC 3 cut(s) 253, 337, 508
Bsp19I CCATGG 1 cut(s) 176
BspACI CCGC 1 cut(s) 360
BspANI GGCC 1 cut(s) 466
BspEI TCCGGA 2 cut(s) 194, 484
BspHI TCATGA 1 cut(s) 511
BspPI GGATC 2 cut(s) 248, 332
BspTI CTTAAG 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 3 cut(s) 253, 337, 508
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 2 cut(s) 192, 491
BstAFI CTTAAG 1 cut(s) 110
BstDSI CCRYGG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 3 cut(s) 256, 340, 511
BstMBI GATC 3 cut(s) 253, 337, 508
BstNI CCWGG 1 cut(s) 432
BstSCI CCNGG 1 cut(s) 430
BstV1I GCAGC 3 cut(s) 140, 293, 400
BsuRI GGCC 1 cut(s) 466
BtgI CCRYGG 1 cut(s) 176
BtgZI GCGATG 1 cut(s) 324
BtrI CACGTC 1 cut(s) 470
BtsIMutI CAGTG 1 cut(s) 333
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 192, 197
CpoI CGGWCCG 1 cut(s) 192
CspI CGGWCCG 1 cut(s) 192
CviAII CATG 4 cut(s) 65, 105, 177, 512
CviJI RGCY 8 cut(s) 16, 109, 117, 131, 160, 209, 430, 466
CviKI_1 RGCY 8 cut(s) 16, 109, 117, 131, 160, 209, 430, 466
DpnI GATC 3 cut(s) 255, 339, 510
DpnII GATC 3 cut(s) 253, 337, 508
EaeI YGGCCR 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 192, 197
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 4 cut(s) 68, 108, 180, 515
FaiI YATR 7 cut(s) 66, 106, 178, 401, 411, 504, 513
FatI CATG 4 cut(s) 64, 104, 176, 511
FauI CCCGC 1 cut(s) 353
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 129, 307, 414
Fsp4HI GCNGC 3 cut(s) 129, 307, 414
GlaI GCGC 1 cut(s) 384
GluI GCNGC 3 cut(s) 129, 307, 414
HaeIII GGCC 1 cut(s) 466
HapII CCGG 3 cut(s) 195, 378, 485
HhaI GCGC 1 cut(s) 385
Hin1II CATG 4 cut(s) 68, 108, 180, 515
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HinfI GANTC 1 cut(s) 374
HpaII CCGG 3 cut(s) 195, 378, 485
Hpy166II GTNNAC 5 cut(s) 152, 173, 325, 353, 494
Hpy188I TCNGA 2 cut(s) 205, 253
Hpy188III TCNNGA 5 cut(s) 77, 195, 280, 485, 512
Hpy8I GTNNAC 5 cut(s) 152, 173, 325, 353, 494
Hpy99I CGWCG 1 cut(s) 450
HpyAV CCTTC 1 cut(s) 489
HpyCH4III ACNGT 2 cut(s) 192, 491
HpyCH4IV ACGT 2 cut(s) 439, 469
HpyCH4V TGCA 1 cut(s) 186
HpySE526I ACGT 2 cut(s) 439, 469
Hsp92II CATG 4 cut(s) 68, 108, 180, 515
HspAI GCGC 1 cut(s) 383
Kpn2I TCCGGA 2 cut(s) 194, 484
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 3 cut(s) 253, 337, 508
LmnI GCTCC 1 cut(s) 421
LpnPI CCDG 5 cut(s) 208, 391, 417, 444, 498
Lsp1109I GCAGC 3 cut(s) 140, 293, 400
MaeII ACGT 2 cut(s) 439, 469
MaeIII GTNAC 4 cut(s) 74, 227, 423, 449
MalI GATC 3 cut(s) 255, 339, 510
MboI GATC 3 cut(s) 253, 337, 508
MboII GAAGA 3 cut(s) 28, 31, 50
MluCI AATT 4 cut(s) 49, 142, 346, 499
MnlI CCTC 5 cut(s) 53, 109, 199, 483, 486
MroI TCCGGA 2 cut(s) 194, 484
MseI TTAA 6 cut(s) 12, 32, 47, 90, 111, 498
MslI CAYNNNNRTG 3 cut(s) 166, 301, 516
MspCI CTTAAG 1 cut(s) 110
MspI CCGG 3 cut(s) 195, 378, 485
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
NcoI CCATGG 1 cut(s) 176
NdeII GATC 3 cut(s) 253, 337, 508
NlaIII CATG 4 cut(s) 68, 108, 180, 515
NmuCI GTSAC 3 cut(s) 74, 423, 449
OliI CACNNNNGTG 1 cut(s) 166
PagI TCATGA 1 cut(s) 511
PcsI WCGNNNNNNNCGW 1 cut(s) 445
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 3 cut(s) 130, 308, 415
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspPI GGNCC 2 cut(s) 192, 197
RseI CAYNNNNRTG 3 cut(s) 166, 301, 516
Rsr2I CGGWCCG 1 cut(s) 192
RsrII CGGWCCG 1 cut(s) 192
SaqAI TTAA 6 cut(s) 12, 32, 47, 90, 111, 498
SatI GCNGC 3 cut(s) 129, 307, 414
Sau3AI GATC 3 cut(s) 253, 337, 508
Sau96I GGNCC 2 cut(s) 192, 197
ScrFI CCNGG 1 cut(s) 432
SetI ASST 6 cut(s) 18, 101, 133, 293, 442, 472
SinI GGWCC 2 cut(s) 192, 197
SmiMI CAYNNNNRTG 3 cut(s) 166, 301, 516
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 49, 142, 346, 499
SsiI CCGC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 430
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 2 cut(s) 192, 491
TaiI ACGT 2 cut(s) 442, 472
TaqI TCGA 1 cut(s) 211
TaqII GACCGA 1 cut(s) 62
TasI AATT 4 cut(s) 49, 142, 346, 499
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 6 cut(s) 12, 32, 47, 90, 111, 498
Tru9I TTAA 6 cut(s) 12, 32, 47, 90, 111, 498
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 3 cut(s) 74, 423, 449
TseI GCWGC 3 cut(s) 128, 306, 413
Tsp45I GTSAC 3 cut(s) 74, 423, 449
TspDTI ATGAA 2 cut(s) 50, 449
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 1 cut(s) 340
Vha464I CTTAAG 1 cut(s) 110
VpaK11BI GGWCC 2 cut(s) 192, 197
XapI RAATTY 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.