Rh5CG313000

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017921
Physical Location & Seq
Reverse (-)
34513784 .. 34514669
886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG313000.1

Sequence Viewer

Length: 606 bp
ATGCAAGAAAAGCATGAACAAGGTATTAAACAATCAAATCGAAACGATCTCCCAGCTTTTTGGTTACTTGTATATATCGCATCAGTGCCCTCCTCTATCTTCATCTTGTGCATGCATCTTAAAGCAATGCTGCTCGCCAAGGTAGAGATTGTCATTTCAGTACTTCTACTATCCCTAACCCTAGTTGTGATAGCAGAAGGGGATGATATTTCTTCGGTAGTGCTACAAAATATCAATGGCAGTTCATCTATGTCGCCATTTTTGAAGAAAATCGTGGAATGGCCTAGACCACGGGAACCAGGATGCCGGAACAGGCCATGGACATGTCGTCGGCCGCGGAAGTTTCCTCGCAGATGGCGGTGTTGTCGAAACCAATGTGTTGACGTGAGCTCAGACGTGAACAATTGCCGGTTTTGTGGATATAGATGCCCTTTAGGATGGCAATGTTGCCGTGGTTTTTGCGTGAATACAAATATCAGCATTTTCAATTGTGGAAGATGTGGGAATAGGTGTCCTTTTGGGGTACTTTGCACCTATGGAATGTGTGGGTATGCTCAACCTTGGCCTCGGCCTCCTTTCCTTTGTCCTCCACCCCCAATGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

201

Amino Acids

22.91

Weight (kDa)

9.16

Isoelectric Point (pI)

55.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 101 - 185 3.6e-23 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 337
AciI CCGC 3 cut(s) 335, 337, 358
AcoI YGGCCR 1 cut(s) 332
AfaI GTAC 2 cut(s) 162, 525
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 2 cut(s) 265, 487
AhdI GACNNNNNGTC 1 cut(s) 327
AjiI CACGTC 2 cut(s) 385, 397
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 2 cut(s) 56, 390
AluI AGCT 2 cut(s) 56, 390
Alw21I GWGCWC 1 cut(s) 392
AoxI GGCC 5 cut(s) 281, 314, 332, 563, 569
ApeKI GCWGC 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 90
BanII GRGCYC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 1 cut(s) 117
BccI CCATC 2 cut(s) 348, 432
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 1 cut(s) 300
BfaI CTAG 3 cut(s) 182, 285, 604
BisI GCNGC 2 cut(s) 131, 335
BlsI GCNGC 2 cut(s) 132, 336
BmcAI AGTACT 1 cut(s) 162
Bme1390I CCNGG 1 cut(s) 300
BmeRI GACNNNNNGTC 1 cut(s) 327
BmgBI CACGTC 2 cut(s) 385, 397
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 4 cut(s) 89, 124, 293, 416
BsaJI CCNNGG 7 cut(s) 138, 290, 317, 335, 451, 560, 566
Bse118I RCCGGY 1 cut(s) 408
Bse3DI GCAATG 2 cut(s) 132, 449
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 7 cut(s) 138, 290, 317, 335, 451, 560, 566
BseGI GGATG 3 cut(s) 208, 308, 443
BseMI GCAATG 2 cut(s) 132, 449
BseMII CTCAG 1 cut(s) 405
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 90
BseX3I CGGCCG 1 cut(s) 332
BseXI GCAGC 1 cut(s) 117
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 335
BshFI GGCC 5 cut(s) 283, 316, 334, 565, 571
BsiEI CGRYCG 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 392
BsiSI CCGG 2 cut(s) 307, 409
BsnI GGCC 5 cut(s) 283, 316, 334, 565, 571
Bsp1286I GDGCHC 2 cut(s) 90, 392
Bsp143I GATC 1 cut(s) 46
Bsp19I CCATGG 1 cut(s) 317
BspACI CCGC 3 cut(s) 335, 337, 358
BspANI GGCC 5 cut(s) 283, 316, 334, 565, 571
BspCNI CTCAG 1 cut(s) 404
BspFNI CGCG 1 cut(s) 337
BspLI GGNNCC 1 cut(s) 297
BsrDI GCAATG 2 cut(s) 132, 449
BsrFI RCCGGY 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 7 cut(s) 138, 290, 317, 335, 451, 560, 566
BssMI GATC 1 cut(s) 46
BssT1I CCWWGG 3 cut(s) 138, 317, 560
Bst2UI CCWGG 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 113, 135
BstDEI CTNAG 1 cut(s) 391
BstDSI CCRYGG 4 cut(s) 290, 317, 335, 451
BstF5I GGATG 3 cut(s) 208, 308, 443
BstFNI CGCG 1 cut(s) 337
BstKTI GATC 1 cut(s) 49
BstMBI GATC 1 cut(s) 46
BstMCI CGRYCG 1 cut(s) 335
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 2 cut(s) 115, 327
BstSCI CCNGG 1 cut(s) 298
BstSLI GKGCMC 1 cut(s) 90
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 117
BstXI CCANNNNNNTGG 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 332
BsuRI GGCC 5 cut(s) 283, 316, 334, 565, 571
BtgI CCRYGG 4 cut(s) 290, 317, 335, 451
BtrI CACGTC 2 cut(s) 385, 397
BtsCI GGATG 3 cut(s) 208, 308, 443
BtsIMutI CAGTG 1 cut(s) 90
Cac8I GCNNGC 2 cut(s) 113, 135
Cfr10I RCCGGY 1 cut(s) 408
Cfr42I CCGCGG 1 cut(s) 338
Csp6I GTAC 2 cut(s) 161, 524
CviAII CATG 4 cut(s) 14, 112, 318, 324
CviJI RGCY 7 cut(s) 56, 283, 316, 334, 390, 565, 571
CviKI_1 RGCY 7 cut(s) 56, 283, 316, 334, 390, 565, 571
CviQI GTAC 2 cut(s) 161, 524
DdeI CTNAG 1 cut(s) 391
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 332
EagI CGGCCG 1 cut(s) 332
Eam1105I GACNNNNNGTC 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 332
Eco130I CCWWGG 3 cut(s) 138, 317, 560
Eco24I GRGCYC 1 cut(s) 392
Eco52I CGGCCG 1 cut(s) 332
Eco53kI GAGCTC 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 3 cut(s) 138, 317, 560
EcoT22I ATGCAT 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 3 cut(s) 138, 317, 560
FaeI CATG 4 cut(s) 17, 115, 321, 327
FatI CATG 4 cut(s) 13, 111, 317, 323
Fnu4HI GCNGC 2 cut(s) 131, 335
FokI GGATG 3 cut(s) 215, 315, 450
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 2 cut(s) 131, 335
FspBI CTAG 3 cut(s) 182, 285, 604
GluI GCNGC 2 cut(s) 131, 335
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 5 cut(s) 283, 316, 334, 565, 571
HapII CCGG 2 cut(s) 307, 409
Hin1II CATG 4 cut(s) 17, 115, 321, 327
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HpaII CCGG 2 cut(s) 307, 409
Hpy166II GTNNAC 2 cut(s) 382, 400
Hpy188I TCNGA 1 cut(s) 394
Hpy8I GTNNAC 2 cut(s) 382, 400
Hpy99I CGWCG 1 cut(s) 333
HpyAV CCTTC 1 cut(s) 191
HpyCH4IV ACGT 2 cut(s) 384, 396
HpyCH4V TGCA 4 cut(s) 4, 111, 115, 531
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 391
HpySE526I ACGT 2 cut(s) 384, 396
Hsp92II CATG 4 cut(s) 17, 115, 321, 327
KspI CCGCGG 1 cut(s) 338
Kzo9I GATC 1 cut(s) 46
LpnPI CCDG 6 cut(s) 66, 285, 298, 312, 320, 422
Lsp1109I GCAGC 1 cut(s) 117
LweI GCATC 4 cut(s) 89, 124, 293, 416
MaeI CTAG 3 cut(s) 182, 285, 604
MaeII ACGT 2 cut(s) 384, 396
MaeIII GTNAC 1 cut(s) 63
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 4 cut(s) 91, 204, 277, 507
MfeI CAATTG 2 cut(s) 403, 487
MhlI GDGCHC 2 cut(s) 90, 392
MluCI AATT 2 cut(s) 403, 487
MnlI CCTC 6 cut(s) 100, 103, 357, 576, 582, 597
Mph1103I ATGCAT 1 cut(s) 117
MseI TTAA 2 cut(s) 27, 120
MslI CAYNNNNRTG 1 cut(s) 322
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 2 cut(s) 307, 409
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 2 cut(s) 403, 487
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 10
NcoI CCATGG 1 cut(s) 317
NdeII GATC 1 cut(s) 46
NlaIII CATG 4 cut(s) 17, 115, 321, 327
NlaIV GGNNCC 1 cut(s) 297
NmeAIII GCCGAG 1 cut(s) 547
NsiI ATGCAT 1 cut(s) 117
NspI RCATGY 2 cut(s) 115, 327
PaeI GCATGC 1 cut(s) 115
PciI ACATGT 1 cut(s) 323
PkrI GCNGC 2 cut(s) 132, 336
PscI ACATGT 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 1 cut(s) 297
RsaI GTAC 2 cut(s) 162, 525
RsaNI GTAC 2 cut(s) 161, 524
RseI CAYNNNNRTG 1 cut(s) 322
SacI GAGCTC 1 cut(s) 392
SacII CCGCGG 1 cut(s) 338
SaqAI TTAA 2 cut(s) 27, 120
SatI GCNGC 2 cut(s) 131, 335
Sau3AI GATC 1 cut(s) 46
ScaI AGTACT 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 2 cut(s) 90, 392
SetI ASST 9 cut(s) 25, 58, 144, 387, 392, 399, 512, 536, 562
SfaNI GCATC 4 cut(s) 89, 124, 293, 416
Sfr303I CCGCGG 1 cut(s) 338
SgrBI CCGCGG 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 322
SphI GCATGC 1 cut(s) 115
Sse9I AATT 2 cut(s) 403, 487
SsiI CCGC 3 cut(s) 335, 337, 358
SspMI CTAG 3 cut(s) 182, 285, 604
SstI GAGCTC 1 cut(s) 392
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 3 cut(s) 138, 317, 560
TaiI ACGT 2 cut(s) 387, 399
TaqI TCGA 2 cut(s) 40, 367
TasI AATT 2 cut(s) 403, 487
TatI WGTACW 1 cut(s) 160
TauI GCSGC 1 cut(s) 337
Tru1I TTAA 2 cut(s) 27, 120
Tru9I TTAA 2 cut(s) 27, 120
TscAI CASTG 1 cut(s) 90
TseI GCWGC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 30, 91, 234
TspRI CASTG 1 cut(s) 90
XceI RCATGY 2 cut(s) 115, 327
XspI CTAG 3 cut(s) 182, 285, 604
ZrmI AGTACT 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.