Prupe.4G221300_v2.0.a1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017921
Physical Location & Seq
Reverse (-)
13901818 .. 13902330
513 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G221300.1

Sequence Viewer

Length: 513 bp
ATGCTAGCCGAACTTCACTTTTTCTTTGTACTTGTTCTATTGCTATACCTTCTTGTGATCATAGTTGAAGGCCTAGGGCATTCAAATGCCACTTCTCCATGGCTAAACAAAGTAGCAACGAACTGGCCACGACCACCGCGGCCGGGAGGGTGCCGGCGCCAGCCTTGGGTATGTCGTCAGCAAGGAAACCCTCCGACCAGGTGGCGGTGCTGTAAGGACCGTTGCGTCAACATCAGCTCCGATGTTAACAATTGTGGGCTGTGTCGAATTAGATGTCCATTTAGTTGGCAATGTTGTCGTGGTTTTTGCGTGAACACCAATATTAGCCCTTTTAATTGTGGGAGGTGTGGAAATAGGTGCCCCTTTGGTGTGCTTTGCTTGTATGGCCTGTGTGGCTATGCTCAGCCGACCTTGCCTCCATGGCATTGTCCTCCATGGCCTCGCCCTCCATTCCCCTTTCCTCCTAAGCGACCATGGCCTCCACGACCTCAGCCACCCCAAATTATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

171

Amino Acids

19.49

Weight (kDa)

9.36

Isoelectric Point (pI)

60.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AccB1I GGYRCC 3 cut(s) 150, 156, 357
AccB7I CCANNNNNTGG 1 cut(s) 505
AccII CGCG 1 cut(s) 139
AciI CCGC 3 cut(s) 137, 139, 205
AcoI YGGCCR 2 cut(s) 125, 140
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 4 cut(s) 143, 166, 204, 505
AgsI TTSAA 2 cut(s) 68, 84
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 1 cut(s) 237
AluI AGCT 1 cut(s) 237
AoxI GGCC 6 cut(s) 70, 125, 140, 385, 437, 476
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 144
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 217
AvrII CCTAGG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 127
BanI GGYRCC 3 cut(s) 150, 156, 357
BbvCI CCTCAGC 1 cut(s) 489
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 1 cut(s) 199
BclI TGATCA 1 cut(s) 57
BcnI CCSGG 1 cut(s) 144
BfaI CTAG 2 cut(s) 5, 74
BfoI RGCGCY 1 cut(s) 160
BglI GCCNNNNNGGC 2 cut(s) 393, 421
BisI GCNGC 1 cut(s) 140
BlnI CCTAGG 1 cut(s) 73
BlpI GCTNAGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 141
Bme1390I CCNGG 2 cut(s) 144, 199
Bme18I GGWCC 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 3 cut(s) 152, 158, 359
BmrFI CCNGG 2 cut(s) 144, 199
BmtI GCTAGC 1 cut(s) 8
Bpu10I CCTNAGC 2 cut(s) 465, 489
Bpu1102I GCTNAGC 1 cut(s) 402
BpuMI CCSGG 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 157
BsaJI CCNNGG 7 cut(s) 73, 98, 137, 164, 419, 434, 473
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 400, 430
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 166, 204, 505
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 296
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 7 cut(s) 73, 98, 137, 164, 419, 434, 473
BseLI CCNNNNNNNGG 4 cut(s) 143, 166, 204, 505
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 2 cut(s) 416, 503
BseNI ACTGG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 362
BseX3I CGGCCG 1 cut(s) 140
Bsh1236I CGCG 1 cut(s) 139
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 6 cut(s) 72, 127, 142, 387, 439, 478
BshNI GGYRCC 3 cut(s) 150, 156, 357
BsiEI CGRYCG 1 cut(s) 143
BsiSI CCGG 2 cut(s) 143, 154
BslI CCNNNNNNNGG 4 cut(s) 143, 166, 204, 505
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 6 cut(s) 72, 127, 142, 387, 439, 478
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 1 cut(s) 57
Bsp1720I GCTNAGC 1 cut(s) 402
Bsp19I CCATGG 4 cut(s) 98, 419, 434, 473
BspACI CCGC 3 cut(s) 137, 139, 205
BspANI GGCC 6 cut(s) 72, 127, 142, 387, 439, 478
BspCNI CTCAG 2 cut(s) 415, 502
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 3 cut(s) 152, 158, 359
BspOI GCTAGC 1 cut(s) 8
BspT107I GGYRCC 3 cut(s) 150, 156, 357
BsrDI GCAATG 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 153
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 7 cut(s) 73, 98, 137, 164, 419, 434, 473
BssMI GATC 1 cut(s) 57
BssNI GRCGYC 1 cut(s) 157
BssT1I CCWWGG 6 cut(s) 73, 98, 164, 419, 434, 473
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 221
BstACI GRCGYC 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 6, 155, 161
BstDEI CTNAG 3 cut(s) 402, 465, 489
BstDSI CCRYGG 5 cut(s) 98, 137, 419, 434, 473
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 1 cut(s) 60
BstMBI GATC 1 cut(s) 57
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 5 cut(s) 384, 393, 412, 421, 475
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 2 cut(s) 142, 197
BstSLI GKGCMC 1 cut(s) 362
BstUI CGCG 1 cut(s) 139
BstXI CCANNNNNNTGG 1 cut(s) 285
BstZI CGGCCG 1 cut(s) 140
BsuRI GGCC 6 cut(s) 72, 127, 142, 387, 439, 478
BtgI CCRYGG 5 cut(s) 98, 137, 419, 434, 473
Cac8I GCNNGC 3 cut(s) 6, 155, 161
CfoI GCGC 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 1 cut(s) 217
Cfr42I CCGCGG 1 cut(s) 140
CseI GACGC 1 cut(s) 214
CsiI ACCWGGT 1 cut(s) 197
Csp6I GTAC 1 cut(s) 29
CviAII CATG 4 cut(s) 99, 420, 435, 474
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 3 cut(s) 402, 465, 489
DinI GGCGCC 1 cut(s) 158
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 2 cut(s) 125, 140
EagI CGGCCG 1 cut(s) 140
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 6 cut(s) 73, 98, 164, 419, 434, 473
Eco147I AGGCCT 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 217
Eco52I CGGCCG 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 6 cut(s) 73, 98, 164, 419, 434, 473
EgeI GGCGCC 1 cut(s) 158
EheI GGCGCC 1 cut(s) 158
ErhI CCWWGG 6 cut(s) 73, 98, 164, 419, 434, 473
FaeI CATG 4 cut(s) 102, 423, 438, 477
FatI CATG 4 cut(s) 98, 419, 434, 473
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 1 cut(s) 140
Fsp4HI GCNGC 1 cut(s) 140
FspBI CTAG 2 cut(s) 5, 74
GlaI GCGC 1 cut(s) 158
GluI GCNGC 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 6 cut(s) 72, 127, 142, 387, 439, 478
HapII CCGG 2 cut(s) 143, 154
HgaI GACGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 4 cut(s) 102, 423, 438, 477
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 2 cut(s) 229, 247
HindII GTYRAC 2 cut(s) 229, 247
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 2 cut(s) 143, 154
Hpy166II GTNNAC 3 cut(s) 229, 247, 313
Hpy188I TCNGA 2 cut(s) 195, 241
Hpy8I GTNNAC 3 cut(s) 229, 247, 313
HpyAV CCTTC 2 cut(s) 59, 62
HpyCH4III ACNGT 1 cut(s) 221
HpyF10VI GCNNNNNNNGC 5 cut(s) 384, 393, 412, 421, 475
HpyF3I CTNAG 3 cut(s) 402, 465, 489
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 4 cut(s) 102, 423, 438, 477
HspAI GCGC 1 cut(s) 157
KasI GGCGCC 1 cut(s) 156
KroI GCCGGC 1 cut(s) 153
KroNI GCCGGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 57
KspAI GTTAAC 1 cut(s) 247
KspI CCGCGG 1 cut(s) 140
Kzo9I GATC 1 cut(s) 57
LmnI GCTCC 1 cut(s) 242
LpnPI CCDG 7 cut(s) 109, 156, 167, 173, 184, 211, 401
MabI ACCWGGT 1 cut(s) 197
MaeI CTAG 2 cut(s) 5, 74
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MfeI CAATTG 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 362
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 4 cut(s) 250, 267, 334, 501
MluNI TGGCCA 1 cut(s) 127
Mly113I GGCGCC 1 cut(s) 157
MmeI TCCRAC 1 cut(s) 218
Mox20I TGGCCA 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 153
MscI TGGCCA 1 cut(s) 127
MseI TTAA 2 cut(s) 246, 333
MslI CAYNNNNRTG 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 127
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 2 cut(s) 143, 154
MspR9I CCNGG 2 cut(s) 144, 199
MunI CAATTG 1 cut(s) 250
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 5 cut(s) 384, 393, 412, 421, 475
NaeI GCCGGC 1 cut(s) 155
NarI GGCGCC 1 cut(s) 157
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 4 cut(s) 98, 419, 434, 473
NdeII GATC 1 cut(s) 57
NgoMIV GCCGGC 1 cut(s) 153
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 4 cut(s) 102, 423, 438, 477
NlaIV GGNNCC 3 cut(s) 152, 158, 359
PceI AGGCCT 1 cut(s) 72
PctI GAATGC 1 cut(s) 79
PdiI GCCGGC 1 cut(s) 155
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 1 cut(s) 141
PluTI GGCGCC 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 3 cut(s) 152, 158, 359
PspPI GGNCC 1 cut(s) 217
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 1 cut(s) 84
SacII CCGCGG 1 cut(s) 140
SaqAI TTAA 2 cut(s) 246, 333
SatI GCNGC 1 cut(s) 140
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 1 cut(s) 217
ScrFI CCNGG 2 cut(s) 144, 199
SduI GDGCHC 1 cut(s) 362
SetI ASST 7 cut(s) 51, 203, 239, 347, 359, 413, 490
SexAI ACCWGGT 1 cut(s) 197
SfoI GGCGCC 1 cut(s) 158
Sfr303I CCGCGG 1 cut(s) 140
SgrBI CCGCGG 1 cut(s) 140
SinI GGWCC 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse9I AATT 4 cut(s) 250, 267, 334, 501
SseBI AGGCCT 1 cut(s) 72
SsiI CCGC 3 cut(s) 137, 139, 205
SspDI GGCGCC 1 cut(s) 156
SspI AATATT 1 cut(s) 322
SspMI CTAG 2 cut(s) 5, 74
StuI AGGCCT 1 cut(s) 72
StyD4I CCNGG 2 cut(s) 142, 197
StyI CCWWGG 6 cut(s) 73, 98, 164, 419, 434, 473
TaaI ACNGT 1 cut(s) 221
TaqI TCGA 1 cut(s) 265
TasI AATT 4 cut(s) 250, 267, 334, 501
TatI WGTACW 1 cut(s) 28
TauI GCSGC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 246, 333
Tru9I TTAA 2 cut(s) 246, 333
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 217
XmaJI CCTAGG 1 cut(s) 73
XspI CTAG 2 cut(s) 5, 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.