RchiOBHm_Chr5g0041151

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0017921
Physical Location & Seq
Reverse (-)
35808330 .. 35808851
522 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31959

Sequence Viewer

Length: 522 bp
ATGCCCTCCTCTATCTTCATCTTGTGCATGCATCTTAAAACAATGCTGCTCGCCAAGGTAGAGATTGTCATTTCAGTACTTCTACTATCCCTAACCCTAGTTGTGATAGCAGAAGGGGATGATATTTCTTCGGTAGTGCTACAAAATATCAATGGCAGTTCATCTATGTCGCCATTTTTGAAGAAAATCGTGGAATGGCCTAGACCACGGGAACCGGGATGCCGGAACAGGCCATGGACATGTCGTCGGCCGCGGGAGTTTCCTCGCAGATGGCGGTGTTGTCGAAACCAATGTGTTGACGTGAGCTCAGACGTGAACAATTGCAGGATTTGTGGATATAGATGCCCTTTAGGATGGCAATGTTGCCGTGGTTTTTGCGTGAATACAAATATCAGCATTTTCAATTGTGGAAGATGTGGGAATAGGTGTCCTTTTGGGGTACTTTGCACCTATGGAATGTGTGGGTATGCTCAACCTTGGCCTCGGCCTCCTTTCCTTTGTCCTCCACCCCCAATGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

173

Amino Acids

19.64

Weight (kDa)

9.11

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 73 - 157 2e-23 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 253
AciI CCGC 3 cut(s) 251, 253, 274
AcoI YGGCCR 1 cut(s) 248
AfaI GTAC 2 cut(s) 78, 441
AflIII ACRYGT 1 cut(s) 239
AgsI TTSAA 2 cut(s) 181, 403
AhdI GACNNNNNGTC 1 cut(s) 243
AjiI CACGTC 2 cut(s) 301, 313
AluBI AGCT 1 cut(s) 306
AluI AGCT 1 cut(s) 306
Alw21I GWGCWC 1 cut(s) 308
AoxI GGCC 5 cut(s) 197, 230, 248, 479, 485
ApeKI GCWGC 1 cut(s) 46
AsuC2I CCSGG 1 cut(s) 216
BanII GRGCYC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 1 cut(s) 33
BccI CCATC 2 cut(s) 264, 348
BceAI ACGGC 1 cut(s) 351
BcnI CCSGG 1 cut(s) 216
BfaI CTAG 3 cut(s) 98, 201, 520
BisI GCNGC 2 cut(s) 47, 251
BlsI GCNGC 2 cut(s) 48, 252
BmcAI AGTACT 1 cut(s) 78
Bme1390I CCNGG 1 cut(s) 216
BmeRI GACNNNNNGTC 1 cut(s) 243
BmgBI CACGTC 2 cut(s) 301, 313
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 3 cut(s) 40, 209, 332
BpuMI CCSGG 1 cut(s) 216
BsaJI CCNNGG 7 cut(s) 54, 206, 233, 251, 367, 476, 482
Bse3DI GCAATG 1 cut(s) 365
BseDI CCNNGG 7 cut(s) 54, 206, 233, 251, 367, 476, 482
BseGI GGATG 3 cut(s) 124, 224, 359
BseMI GCAATG 1 cut(s) 365
BseMII CTCAG 1 cut(s) 321
BseX3I CGGCCG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 253
Bsh1285I CGRYCG 1 cut(s) 251
BshFI GGCC 5 cut(s) 199, 232, 250, 481, 487
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 2 cut(s) 215, 223
BsnI GGCC 5 cut(s) 199, 232, 250, 481, 487
Bsp1286I GDGCHC 1 cut(s) 308
Bsp19I CCATGG 1 cut(s) 233
BspACI CCGC 3 cut(s) 251, 253, 274
BspANI GGCC 5 cut(s) 199, 232, 250, 481, 487
BspCNI CTCAG 1 cut(s) 320
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 365
BssECI CCNNGG 7 cut(s) 54, 206, 233, 251, 367, 476, 482
BssT1I CCWWGG 3 cut(s) 54, 233, 476
BstC8I GCNNGC 2 cut(s) 29, 51
BstDEI CTNAG 1 cut(s) 307
BstDSI CCRYGG 4 cut(s) 206, 233, 251, 367
BstF5I GGATG 3 cut(s) 124, 224, 359
BstFNI CGCG 1 cut(s) 253
BstMCI CGRYCG 1 cut(s) 251
BstNSI RCATGY 2 cut(s) 31, 243
BstSCI CCNGG 1 cut(s) 214
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 33
BstZI CGGCCG 1 cut(s) 248
BsuRI GGCC 5 cut(s) 199, 232, 250, 481, 487
BtgI CCRYGG 4 cut(s) 206, 233, 251, 367
BtrI CACGTC 2 cut(s) 301, 313
BtsCI GGATG 3 cut(s) 124, 224, 359
Cac8I GCNNGC 2 cut(s) 29, 51
Cfr42I CCGCGG 1 cut(s) 254
Csp6I GTAC 2 cut(s) 77, 440
CviAII CATG 3 cut(s) 28, 234, 240
CviJI RGCY 6 cut(s) 199, 232, 250, 306, 481, 487
CviKI_1 RGCY 6 cut(s) 199, 232, 250, 306, 481, 487
CviQI GTAC 2 cut(s) 77, 440
DdeI CTNAG 1 cut(s) 307
DriI GACNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 248
EagI CGGCCG 1 cut(s) 248
Eam1105I GACNNNNNGTC 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 306
EclXI CGGCCG 1 cut(s) 248
Eco130I CCWWGG 3 cut(s) 54, 233, 476
Eco24I GRGCYC 1 cut(s) 308
Eco52I CGGCCG 1 cut(s) 248
Eco53kI GAGCTC 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 306
EcoT14I CCWWGG 3 cut(s) 54, 233, 476
EcoT22I ATGCAT 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 308
ErhI CCWWGG 3 cut(s) 54, 233, 476
FaeI CATG 3 cut(s) 31, 237, 243
FaiI YATR 7 cut(s) 29, 167, 235, 241, 339, 453, 468
FatI CATG 3 cut(s) 27, 233, 239
FauI CCCGC 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 47, 251
FokI GGATG 3 cut(s) 131, 231, 366
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 2 cut(s) 47, 251
FspBI CTAG 3 cut(s) 98, 201, 520
GluI GCNGC 2 cut(s) 47, 251
HaeIII GGCC 5 cut(s) 199, 232, 250, 481, 487
HapII CCGG 2 cut(s) 215, 223
Hin1II CATG 3 cut(s) 31, 237, 243
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HpaII CCGG 2 cut(s) 215, 223
Hpy166II GTNNAC 2 cut(s) 298, 316
Hpy188I TCNGA 1 cut(s) 310
Hpy8I GTNNAC 2 cut(s) 298, 316
Hpy99I CGWCG 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 107
HpyCH4IV ACGT 2 cut(s) 300, 312
HpyCH4V TGCA 4 cut(s) 27, 31, 324, 447
HpyF3I CTNAG 1 cut(s) 307
HpySE526I ACGT 2 cut(s) 300, 312
Hsp92II CATG 3 cut(s) 31, 237, 243
KspI CCGCGG 1 cut(s) 254
LpnPI CCDG 4 cut(s) 214, 228, 236, 310
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 3 cut(s) 40, 209, 332
MaeI CTAG 3 cut(s) 98, 201, 520
MaeII ACGT 2 cut(s) 300, 312
MboII GAAGA 4 cut(s) 7, 120, 193, 423
MfeI CAATTG 2 cut(s) 319, 403
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 2 cut(s) 319, 403
MnlI CCTC 6 cut(s) 16, 19, 273, 492, 498, 513
Mph1103I ATGCAT 1 cut(s) 33
MseI TTAA 1 cut(s) 36
MslI CAYNNNNRTG 1 cut(s) 238
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 2 cut(s) 215, 223
MspR9I CCNGG 1 cut(s) 216
MunI CAATTG 2 cut(s) 319, 403
MvnI CGCG 1 cut(s) 253
NciI CCSGG 1 cut(s) 216
NcoI CCATGG 1 cut(s) 233
NlaIII CATG 3 cut(s) 31, 237, 243
NlaIV GGNNCC 1 cut(s) 213
NmeAIII GCCGAG 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 33
NspI RCATGY 2 cut(s) 31, 243
PaeI GCATGC 1 cut(s) 31
PciI ACATGT 1 cut(s) 239
PkrI GCNGC 2 cut(s) 48, 252
PscI ACATGT 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 213
RsaI GTAC 2 cut(s) 78, 441
RsaNI GTAC 2 cut(s) 77, 440
RseI CAYNNNNRTG 1 cut(s) 238
SacI GAGCTC 1 cut(s) 308
SacII CCGCGG 1 cut(s) 254
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 2 cut(s) 47, 251
ScaI AGTACT 1 cut(s) 78
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 1 cut(s) 308
SetI ASST 7 cut(s) 60, 303, 308, 315, 428, 452, 478
SfaNI GCATC 3 cut(s) 40, 209, 332
Sfr303I CCGCGG 1 cut(s) 254
SgrBI CCGCGG 1 cut(s) 254
SmiMI CAYNNNNRTG 1 cut(s) 238
SphI GCATGC 1 cut(s) 31
Sse9I AATT 2 cut(s) 319, 403
SsiI CCGC 3 cut(s) 251, 253, 274
SspMI CTAG 3 cut(s) 98, 201, 520
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 3 cut(s) 54, 233, 476
TaiI ACGT 2 cut(s) 303, 315
TaqI TCGA 1 cut(s) 283
TasI AATT 2 cut(s) 319, 403
TatI WGTACW 1 cut(s) 76
TauI GCSGC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TseI GCWGC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 7, 150
XceI RCATGY 2 cut(s) 31, 243
XspI CTAG 3 cut(s) 98, 201, 520
ZrmI AGTACT 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.