Rh5BG280800

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017921
Physical Location & Seq
Reverse (-)
35461869 .. 35462757
889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG280800.1

Sequence Viewer

Length: 597 bp
ATGCACAGCCATAAACCCATGCAAGAAAAGCATGAACAAGGAATTAAACAATCAAATCGAAACGATCTCCCAGCTTTTTGGTTACTTATATATATCGCATCAATGCCCTCCTCTATCTTCATCTTGTTCATGCATCTTAAAACAATGCTGCTCGCCAAGGTAGAGATTGTCATTTCAGTACTTCTACTATCCCTAACCCTAGTTGTGATAGCAGAAGGGGATGATATTTCTTCGGTAGTGCTACAAAATATCAATGGCAGTTCATCTATGTCGCCATTTTTGAAGAAAATCGTGGAATGGCCTAGACCACGGGAACCGGGATGCCGGAACAGGCCATGGACATGTCGTCGGCCGCGGGAGTTTCCTCGCAGATGGCGGTGTTGTCGAAACCAATGTGTTGACGTGAGCTCAGACGTGAACAATTGCAGGTTTTGTGGATATAGATGCCCTTTAGGATGGCAATGTTGCCGTGGTTTTTGCGTGAATACAAATATCAGCATTTTCAATTGTGGAAGATGTGGGAATAGGTGTCCTTTTGGGGTACTTTGCACCTATGGAATGTGTGGGTATGCTCAACCTTGGCCTCGGTCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

198

Amino Acids

22.74

Weight (kDa)

9.26

Isoelectric Point (pI)

57.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 107 - 191 2.5e-23 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 417
AccII CGCG 1 cut(s) 355
AciI CCGC 3 cut(s) 353, 355, 376
AcoI YGGCCR 1 cut(s) 350
AfaI GTAC 2 cut(s) 180, 543
AflIII ACRYGT 1 cut(s) 341
AgsI TTSAA 2 cut(s) 283, 505
AhdI GACNNNNNGTC 1 cut(s) 345
AjiI CACGTC 2 cut(s) 403, 415
AluBI AGCT 2 cut(s) 74, 408
AluI AGCT 2 cut(s) 74, 408
Alw21I GWGCWC 1 cut(s) 410
AoxI GGCC 4 cut(s) 299, 332, 350, 581
ApeKI GCWGC 1 cut(s) 148
AsuC2I CCSGG 1 cut(s) 318
BanII GRGCYC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 410
BbvI GCAGC 1 cut(s) 135
BccI CCATC 2 cut(s) 366, 450
BceAI ACGGC 1 cut(s) 453
BcnI CCSGG 1 cut(s) 318
BfaI CTAG 2 cut(s) 200, 303
BfuAI ACCTGC 1 cut(s) 417
BisI GCNGC 2 cut(s) 149, 353
BlsI GCNGC 2 cut(s) 150, 354
BmcAI AGTACT 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 318
BmeRI GACNNNNNGTC 1 cut(s) 345
BmgBI CACGTC 2 cut(s) 403, 415
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 4 cut(s) 107, 142, 311, 434
BpuMI CCSGG 1 cut(s) 318
BsaJI CCNNGG 7 cut(s) 156, 308, 335, 353, 469, 578, 584
Bse3DI GCAATG 1 cut(s) 467
BseDI CCNNGG 7 cut(s) 156, 308, 335, 353, 469, 578, 584
BseGI GGATG 3 cut(s) 226, 326, 461
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 423
BseRI GAGGAG 1 cut(s) 100
BseX3I CGGCCG 1 cut(s) 350
BseXI GCAGC 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 355
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 4 cut(s) 301, 334, 352, 583
BsiEI CGRYCG 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 410
BsiSI CCGG 2 cut(s) 317, 325
BsnI GGCC 4 cut(s) 301, 334, 352, 583
Bsp1286I GDGCHC 1 cut(s) 410
Bsp143I GATC 1 cut(s) 64
Bsp19I CCATGG 1 cut(s) 335
BspACI CCGC 3 cut(s) 353, 355, 376
BspANI GGCC 4 cut(s) 301, 334, 352, 583
BspCNI CTCAG 1 cut(s) 422
BspFNI CGCG 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 315
BspMI ACCTGC 1 cut(s) 417
BsrDI GCAATG 1 cut(s) 467
BssECI CCNNGG 7 cut(s) 156, 308, 335, 353, 469, 578, 584
BssMI GATC 1 cut(s) 64
BssT1I CCWWGG 3 cut(s) 156, 335, 578
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 409
BstDSI CCRYGG 4 cut(s) 308, 335, 353, 469
BstF5I GGATG 3 cut(s) 226, 326, 461
BstFNI CGCG 1 cut(s) 355
BstKTI GATC 1 cut(s) 67
BstMBI GATC 1 cut(s) 64
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 316
BstUI CGCG 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 135
BstXI CCANNNNNNTGG 1 cut(s) 78
BstZI CGGCCG 1 cut(s) 350
BsuRI GGCC 4 cut(s) 301, 334, 352, 583
BtgI CCRYGG 4 cut(s) 308, 335, 353, 469
BtrI CACGTC 2 cut(s) 403, 415
BtsCI GGATG 3 cut(s) 226, 326, 461
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 153
Cfr42I CCGCGG 1 cut(s) 356
Csp6I GTAC 2 cut(s) 179, 542
CviAII CATG 5 cut(s) 19, 32, 130, 336, 342
CviJI RGCY 7 cut(s) 9, 74, 301, 334, 352, 408, 583
CviKI_1 RGCY 7 cut(s) 9, 74, 301, 334, 352, 408, 583
CviQI GTAC 2 cut(s) 179, 542
DdeI CTNAG 1 cut(s) 409
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
DriI GACNNNNNGTC 1 cut(s) 345
EaeI YGGCCR 1 cut(s) 350
EagI CGGCCG 1 cut(s) 350
Eam1105I GACNNNNNGTC 1 cut(s) 345
Ecl136II GAGCTC 1 cut(s) 408
EclXI CGGCCG 1 cut(s) 350
Eco130I CCWWGG 3 cut(s) 156, 335, 578
Eco24I GRGCYC 1 cut(s) 410
Eco52I CGGCCG 1 cut(s) 350
Eco53kI GAGCTC 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 408
EcoT14I CCWWGG 3 cut(s) 156, 335, 578
EcoT22I ATGCAT 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 3 cut(s) 156, 335, 578
FaeI CATG 5 cut(s) 22, 35, 133, 339, 345
FatI CATG 5 cut(s) 18, 31, 129, 335, 341
FauI CCCGC 1 cut(s) 348
Fnu4HI GCNGC 2 cut(s) 149, 353
FokI GGATG 3 cut(s) 233, 333, 468
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 2 cut(s) 149, 353
FspBI CTAG 2 cut(s) 200, 303
GluI GCNGC 2 cut(s) 149, 353
GsaI CCCAGC 1 cut(s) 74
HaeIII GGCC 4 cut(s) 301, 334, 352, 583
HapII CCGG 2 cut(s) 317, 325
Hin1II CATG 5 cut(s) 22, 35, 133, 339, 345
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HpaII CCGG 2 cut(s) 317, 325
Hpy166II GTNNAC 2 cut(s) 400, 418
Hpy188I TCNGA 1 cut(s) 412
Hpy8I GTNNAC 2 cut(s) 400, 418
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 1 cut(s) 209
HpyCH4IV ACGT 2 cut(s) 402, 414
HpyCH4V TGCA 5 cut(s) 4, 22, 133, 426, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 28
HpyF3I CTNAG 1 cut(s) 409
HpySE526I ACGT 2 cut(s) 402, 414
Hsp92II CATG 5 cut(s) 22, 35, 133, 339, 345
KspI CCGCGG 1 cut(s) 356
Kzo9I GATC 1 cut(s) 64
LpnPI CCDG 5 cut(s) 84, 316, 330, 338, 412
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 4 cut(s) 107, 142, 311, 434
MaeI CTAG 2 cut(s) 200, 303
MaeII ACGT 2 cut(s) 402, 414
MaeIII GTNAC 1 cut(s) 81
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 4 cut(s) 109, 222, 295, 525
MfeI CAATTG 2 cut(s) 421, 505
MhlI GDGCHC 1 cut(s) 410
MluCI AATT 3 cut(s) 42, 421, 505
MnlI CCTC 4 cut(s) 118, 121, 375, 594
Mph1103I ATGCAT 1 cut(s) 135
MseI TTAA 3 cut(s) 45, 138, 595
MslI CAYNNNNRTG 1 cut(s) 340
MspA1I CMGCKG 1 cut(s) 355
MspI CCGG 2 cut(s) 317, 325
MspR9I CCNGG 1 cut(s) 318
MunI CAATTG 2 cut(s) 421, 505
MvnI CGCG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 28
NciI CCSGG 1 cut(s) 318
NcoI CCATGG 1 cut(s) 335
NdeII GATC 1 cut(s) 64
NlaIII CATG 5 cut(s) 22, 35, 133, 339, 345
NlaIV GGNNCC 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 135
NspI RCATGY 1 cut(s) 345
PciI ACATGT 1 cut(s) 341
PkrI GCNGC 2 cut(s) 150, 354
PscI ACATGT 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 410
PspFI CCCAGC 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 315
RsaI GTAC 2 cut(s) 180, 543
RsaNI GTAC 2 cut(s) 179, 542
RseI CAYNNNNRTG 1 cut(s) 340
SacI GAGCTC 1 cut(s) 410
SacII CCGCGG 1 cut(s) 356
SaqAI TTAA 3 cut(s) 45, 138, 595
SatI GCNGC 2 cut(s) 149, 353
Sau3AI GATC 1 cut(s) 64
ScaI AGTACT 1 cut(s) 180
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 1 cut(s) 410
SetI ASST 9 cut(s) 76, 162, 405, 410, 417, 431, 530, 554, 580
SfaNI GCATC 4 cut(s) 107, 142, 311, 434
Sfr303I CCGCGG 1 cut(s) 356
SgrBI CCGCGG 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 340
Sse9I AATT 3 cut(s) 42, 421, 505
SsiI CCGC 3 cut(s) 353, 355, 376
SspMI CTAG 2 cut(s) 200, 303
SstI GAGCTC 1 cut(s) 410
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 3 cut(s) 156, 335, 578
TaiI ACGT 2 cut(s) 405, 417
TaqI TCGA 2 cut(s) 58, 385
TaqII GACCGA 1 cut(s) 576
TasI AATT 3 cut(s) 42, 421, 505
TatI WGTACW 1 cut(s) 178
TauI GCSGC 1 cut(s) 355
Tru1I TTAA 3 cut(s) 45, 138, 595
Tru9I TTAA 3 cut(s) 45, 138, 595
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 4 cut(s) 48, 109, 118, 252
XceI RCATGY 1 cut(s) 345
XspI CTAG 2 cut(s) 200, 303
ZrmI AGTACT 1 cut(s) 180
Zsp2I ATGCAT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.