RLG00000034030

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017921
Physical Location & Seq
Reverse (-)
35550255 .. 35550734
480 bp
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UTR
Exon/CDS
Intron
RLM00000034030

Sequence Viewer

Length: 480 bp
ATGCTGCTCGCCAAGGGGGAGATTGTCATTTCAGTACTTCTACTATCCCTAACCCTAGTTGTGATAGCAGAAGGGGATGATATTTCTTCGGTAGTGCTACAAAATATCAATGGCAGTTCATCTATGTCGCCATTTTTGAAGAAAATCGTGGAATGGCCTAGACCACGGGAACCGGGATGCCGGAACAGGCCATGGACATGTCGTCGGCCAAGGGAGTTTCCTCGCCGATGGCGGTTTTGTCAAAACCAATGTGTTGACGTGAGCTCAGACGTGAACAATTGCAGGTTTTGTGGATATAGATGCCCTTTTGGATGGCAATGTTGCCGTGGTTTTTGCGTGAATACAAATATCAGCATTTTCAATTGTGGAAGATGTGGGAATAGGTGTCCTTTTGGGGTACTTTGCACCTATGGAATGTGTGGGTATGCTCAACCTCGGCCTCGGTCTCCTTTCCTTTATCCTCCACCCCCAATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

160

Amino Acids

18.08

Weight (kDa)

9.08

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 59 - 143 3.6e-23 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 273
AciI CCGC 1 cut(s) 232
AcoI YGGCCR 1 cut(s) 206
AfaI GTAC 2 cut(s) 36, 399
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 2 cut(s) 139, 361
AhdI GACNNNNNGTC 1 cut(s) 201
AjiI CACGTC 2 cut(s) 259, 271
AluBI AGCT 1 cut(s) 264
AluI AGCT 1 cut(s) 264
Alw21I GWGCWC 1 cut(s) 266
Alw26I GTCTC 1 cut(s) 450
AoxI GGCC 4 cut(s) 155, 188, 206, 437
ApeKI GCWGC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 174
BanII GRGCYC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 266
BccI CCATC 2 cut(s) 222, 306
BceAI ACGGC 1 cut(s) 309
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 3 cut(s) 56, 159, 478
BfuAI ACCTGC 1 cut(s) 273
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 174
BmeRI GACNNNNNGTC 1 cut(s) 201
BmgBI CACGTC 2 cut(s) 259, 271
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 167, 290
BpuMI CCSGG 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 7 cut(s) 12, 164, 191, 209, 325, 434, 440
Bse3DI GCAATG 1 cut(s) 323
BseDI CCNNGG 7 cut(s) 12, 164, 191, 209, 325, 434, 440
BseGI GGATG 3 cut(s) 82, 182, 317
BseMI GCAATG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 279
BshFI GGCC 4 cut(s) 157, 190, 208, 439
BsiHKAI GWGCWC 1 cut(s) 266
BsiSI CCGG 2 cut(s) 173, 181
BsmAI GTCTC 1 cut(s) 450
BsnI GGCC 4 cut(s) 157, 190, 208, 439
Bso31I GGTCTC 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 266
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 1 cut(s) 232
BspANI GGCC 4 cut(s) 157, 190, 208, 439
BspCNI CTCAG 1 cut(s) 278
BspLI GGNNCC 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 450
BsrDI GCAATG 1 cut(s) 323
BssECI CCNNGG 7 cut(s) 12, 164, 191, 209, 325, 434, 440
BssT1I CCWWGG 3 cut(s) 12, 191, 209
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 1 cut(s) 265
BstDSI CCRYGG 3 cut(s) 164, 191, 325
BstF5I GGATG 3 cut(s) 82, 182, 317
BstMAI GTCTC 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 172
BsuRI GGCC 4 cut(s) 157, 190, 208, 439
BtgI CCRYGG 3 cut(s) 164, 191, 325
BtrI CACGTC 2 cut(s) 259, 271
BtsCI GGATG 3 cut(s) 82, 182, 317
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 9
Csp6I GTAC 2 cut(s) 35, 398
CviAII CATG 2 cut(s) 192, 198
CviJI RGCY 5 cut(s) 157, 190, 208, 264, 439
CviKI_1 RGCY 5 cut(s) 157, 190, 208, 264, 439
CviQI GTAC 2 cut(s) 35, 398
DdeI CTNAG 1 cut(s) 265
DriI GACNNNNNGTC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 12, 191, 209
Eco24I GRGCYC 1 cut(s) 266
Eco31I GGTCTC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 264
EcoT14I CCWWGG 3 cut(s) 12, 191, 209
EcoT38I GRGCYC 1 cut(s) 266
ErhI CCWWGG 3 cut(s) 12, 191, 209
FaeI CATG 2 cut(s) 195, 201
FaiI YATR 6 cut(s) 125, 193, 199, 297, 411, 426
FatI CATG 2 cut(s) 191, 197
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 3 cut(s) 89, 189, 324
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 3 cut(s) 56, 159, 478
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 4 cut(s) 157, 190, 208, 439
HapII CCGG 2 cut(s) 173, 181
Hin1II CATG 2 cut(s) 195, 201
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HpaII CCGG 2 cut(s) 173, 181
Hpy166II GTNNAC 2 cut(s) 256, 274
Hpy188I TCNGA 1 cut(s) 268
Hpy8I GTNNAC 2 cut(s) 256, 274
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 1 cut(s) 65
HpyCH4IV ACGT 2 cut(s) 258, 270
HpyCH4V TGCA 2 cut(s) 282, 405
HpyF3I CTNAG 1 cut(s) 265
HpySE526I ACGT 2 cut(s) 258, 270
Hsp92II CATG 2 cut(s) 195, 201
LpnPI CCDG 4 cut(s) 172, 186, 194, 268
LweI GCATC 2 cut(s) 167, 290
MaeI CTAG 3 cut(s) 56, 159, 478
MaeII ACGT 2 cut(s) 258, 270
MboII GAAGA 3 cut(s) 78, 151, 381
MfeI CAATTG 2 cut(s) 277, 361
MhlI GDGCHC 1 cut(s) 266
MluCI AATT 2 cut(s) 277, 361
MnlI CCTC 4 cut(s) 231, 444, 450, 471
MslI CAYNNNNRTG 1 cut(s) 196
MspI CCGG 2 cut(s) 173, 181
MspR9I CCNGG 1 cut(s) 174
MunI CAATTG 2 cut(s) 277, 361
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 191
NlaIII CATG 2 cut(s) 195, 201
NlaIV GGNNCC 1 cut(s) 171
NmeAIII GCCGAG 1 cut(s) 415
NspI RCATGY 1 cut(s) 201
PciI ACATGT 1 cut(s) 197
PkrI GCNGC 1 cut(s) 6
PscI ACATGT 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 171
RsaI GTAC 2 cut(s) 36, 399
RsaNI GTAC 2 cut(s) 35, 398
RseI CAYNNNNRTG 1 cut(s) 196
SacI GAGCTC 1 cut(s) 266
SatI GCNGC 1 cut(s) 5
ScaI AGTACT 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 266
SetI ASST 7 cut(s) 261, 266, 273, 287, 386, 410, 436
SfaNI GCATC 2 cut(s) 167, 290
SmiMI CAYNNNNRTG 1 cut(s) 196
Sse9I AATT 2 cut(s) 277, 361
SsiI CCGC 1 cut(s) 232
SspMI CTAG 3 cut(s) 56, 159, 478
SstI GAGCTC 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 3 cut(s) 12, 191, 209
TaiI ACGT 2 cut(s) 261, 273
TaqII GACCGA 1 cut(s) 432
TasI AATT 2 cut(s) 277, 361
TatI WGTACW 1 cut(s) 34
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 108
XceI RCATGY 1 cut(s) 201
XspI CTAG 3 cut(s) 56, 159, 478
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.