Rroxscaffold_1G00039520

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017921
Physical Location & Seq
Forward (+)
57181523 .. 57182002
480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039520.1

Sequence Viewer

Length: 480 bp
ATGCTGCTCGCCAAGGTAGAGATTGTCATTTCAGTACTTCTACCATCCCTAACCCTAGTTGTGATAGCAGAAGGGGATGATATTTCTTCGGTAGTGCTACAAAATATCAATGGCAGTTCATCTATGTCGCCATTTTTGAAGAAAATCGTGGAATGGCCTAGACCACGGGAACCGGGATGCCGGAACAGGCCATGGACATGTCGTCGGCCGCGGGAGTTTCCTAGCAGATGGCGGTGTTGTCGAAACCAATGTGTTGACGTGAGCTCAGACGCGAACAATTGCAGGTTTTGTGGATATAGATGCCCTTTAGGATGGCAATGTTGCCGTGGTTTTTGTGTGAATACAAATATCAGCATTTTCAATTGTGGAAGATGTGGGAATAGGTGTCCTTTTGGGGTACTTTGCACCTATGGAATGTGTGGGTATGCTCAACCTTGGCCTCGGCTTCCTTTCCTTTGTCCTCCACCCCCAATGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

159

Amino Acids

17.97

Weight (kDa)

8.9

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 59 - 143 9.5e-24 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 273
AccII CGCG 2 cut(s) 211, 272
AciI CCGC 3 cut(s) 209, 211, 232
AcoI YGGCCR 1 cut(s) 206
AfaI GTAC 2 cut(s) 36, 399
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 2 cut(s) 139, 361
AhdI GACNNNNNGTC 1 cut(s) 201
AjiI CACGTC 1 cut(s) 259
AluBI AGCT 1 cut(s) 264
AluI AGCT 1 cut(s) 264
Alw21I GWGCWC 1 cut(s) 266
AoxI GGCC 4 cut(s) 155, 188, 206, 437
ApeKI GCWGC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 174
BanII GRGCYC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 266
BccI CCATC 3 cut(s) 52, 222, 306
BceAI ACGGC 1 cut(s) 309
BcnI CCSGG 1 cut(s) 174
BfaI CTAG 4 cut(s) 56, 159, 222, 478
BfuAI ACCTGC 1 cut(s) 273
BisI GCNGC 2 cut(s) 5, 209
BlsI GCNGC 2 cut(s) 6, 210
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 174
BmeRI GACNNNNNGTC 1 cut(s) 201
BmgBI CACGTC 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 167, 290
BpuMI CCSGG 1 cut(s) 174
BsaJI CCNNGG 7 cut(s) 12, 164, 191, 209, 325, 434, 440
Bse3DI GCAATG 1 cut(s) 323
BseDI CCNNGG 7 cut(s) 12, 164, 191, 209, 325, 434, 440
BseGI GGATG 4 cut(s) 44, 82, 182, 317
BseMI GCAATG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 279
BseX3I CGGCCG 1 cut(s) 206
Bsh1236I CGCG 2 cut(s) 211, 272
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 4 cut(s) 157, 190, 208, 439
BsiEI CGRYCG 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 266
BsiSI CCGG 2 cut(s) 173, 181
BsnI GGCC 4 cut(s) 157, 190, 208, 439
Bsp1286I GDGCHC 1 cut(s) 266
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 3 cut(s) 209, 211, 232
BspANI GGCC 4 cut(s) 157, 190, 208, 439
BspCNI CTCAG 1 cut(s) 278
BspFNI CGCG 2 cut(s) 211, 272
BspLI GGNNCC 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 323
BssECI CCNNGG 7 cut(s) 12, 164, 191, 209, 325, 434, 440
BssT1I CCWWGG 3 cut(s) 12, 191, 434
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 1 cut(s) 265
BstDSI CCRYGG 4 cut(s) 164, 191, 209, 325
BstF5I GGATG 4 cut(s) 44, 82, 182, 317
BstFNI CGCG 2 cut(s) 211, 272
BstMCI CGRYCG 1 cut(s) 209
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 172
BstUI CGCG 2 cut(s) 211, 272
BstZI CGGCCG 1 cut(s) 206
BsuRI GGCC 4 cut(s) 157, 190, 208, 439
BtgI CCRYGG 4 cut(s) 164, 191, 209, 325
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 4 cut(s) 44, 82, 182, 317
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 9
Cfr42I CCGCGG 1 cut(s) 212
CseI GACGC 1 cut(s) 278
Csp6I GTAC 2 cut(s) 35, 398
CviAII CATG 2 cut(s) 192, 198
CviJI RGCY 6 cut(s) 157, 190, 208, 264, 439, 445
CviKI_1 RGCY 6 cut(s) 157, 190, 208, 264, 439, 445
CviQI GTAC 2 cut(s) 35, 398
DdeI CTNAG 1 cut(s) 265
DriI GACNNNNNGTC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 206
EagI CGGCCG 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 264
EclXI CGGCCG 1 cut(s) 206
Eco130I CCWWGG 3 cut(s) 12, 191, 434
Eco24I GRGCYC 1 cut(s) 266
Eco52I CGGCCG 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 264
EcoT14I CCWWGG 3 cut(s) 12, 191, 434
EcoT38I GRGCYC 1 cut(s) 266
ErhI CCWWGG 3 cut(s) 12, 191, 434
FaeI CATG 2 cut(s) 195, 201
FaiI YATR 6 cut(s) 125, 193, 199, 297, 411, 426
FatI CATG 2 cut(s) 191, 197
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 5, 209
FokI GGATG 4 cut(s) 31, 89, 189, 324
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 5, 209
FspBI CTAG 4 cut(s) 56, 159, 222, 478
GluI GCNGC 2 cut(s) 5, 209
HaeIII GGCC 4 cut(s) 157, 190, 208, 439
HapII CCGG 2 cut(s) 173, 181
HgaI GACGC 1 cut(s) 278
Hin1II CATG 2 cut(s) 195, 201
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HpaII CCGG 2 cut(s) 173, 181
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 1 cut(s) 268
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 282, 405
HpyF3I CTNAG 1 cut(s) 265
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 2 cut(s) 195, 201
KspI CCGCGG 1 cut(s) 212
LpnPI CCDG 4 cut(s) 172, 186, 194, 268
LweI GCATC 2 cut(s) 167, 290
MaeI CTAG 4 cut(s) 56, 159, 222, 478
MaeII ACGT 1 cut(s) 258
MboII GAAGA 3 cut(s) 78, 151, 381
MfeI CAATTG 2 cut(s) 277, 361
MhlI GDGCHC 1 cut(s) 266
MluCI AATT 2 cut(s) 277, 361
MnlI CCTC 2 cut(s) 450, 471
MslI CAYNNNNRTG 1 cut(s) 196
MspA1I CMGCKG 1 cut(s) 211
MspI CCGG 2 cut(s) 173, 181
MspR9I CCNGG 1 cut(s) 174
MunI CAATTG 2 cut(s) 277, 361
MvnI CGCG 2 cut(s) 211, 272
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 191
NlaIII CATG 2 cut(s) 195, 201
NlaIV GGNNCC 1 cut(s) 171
NmeAIII GCCGAG 1 cut(s) 421
NspI RCATGY 1 cut(s) 201
PciI ACATGT 1 cut(s) 197
PkrI GCNGC 2 cut(s) 6, 210
PscI ACATGT 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 171
RsaI GTAC 2 cut(s) 36, 399
RsaNI GTAC 2 cut(s) 35, 398
RseI CAYNNNNRTG 1 cut(s) 196
SacI GAGCTC 1 cut(s) 266
SacII CCGCGG 1 cut(s) 212
SatI GCNGC 2 cut(s) 5, 209
ScaI AGTACT 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 266
SetI ASST 7 cut(s) 18, 261, 266, 287, 386, 410, 436
SfaNI GCATC 2 cut(s) 167, 290
Sfr303I CCGCGG 1 cut(s) 212
SgrBI CCGCGG 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 196
Sse9I AATT 2 cut(s) 277, 361
SsiI CCGC 3 cut(s) 209, 211, 232
SspMI CTAG 4 cut(s) 56, 159, 222, 478
SstI GAGCTC 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 3 cut(s) 12, 191, 434
TaiI ACGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 241
TasI AATT 2 cut(s) 277, 361
TatI WGTACW 1 cut(s) 34
TauI GCSGC 1 cut(s) 211
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 108
XceI RCATGY 1 cut(s) 201
XspI CTAG 4 cut(s) 56, 159, 222, 478
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.