MD11G1211300.v1.1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0017921
Physical Location & Seq
Forward (+)
30912416 .. 30912895
480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1211300.v1.1.491

Sequence Viewer

Length: 480 bp
ATGCTGGTTTCCAAACTTCAATTTGTGATCGTTTCCACACTTCTACTATTGCTATCGCTTTTTGTGAGCACAATCCAAGGGCATTATTCCAATGCCACCACAACTGCTGCTGCCGGTGCATCCACGTCACCGTGGCTGAAAAAAGTAACAACGAACTGGCCACGCCCACCAAGGCCACGAGGGTGCCGGGGCCGGCCTTGGATATGTCGTAGGAAGGAAGAGTTTCCGGAAAAATGGCCGGATCCGTTTCGGCAAAGATGGCGGTGCTGTAGAGATCGTTGTGTTGACGTCAGCTTCGATGTTTACAATTGTGGGTTGTGTGGAATCAGATGCCCGTTTAGTTGGCAATGCTGTGGTGGTTTTTGCAGTAATACCAATATTAGCCCATTTAATTGTGGGAGATGTGGAAACAGGTGCCCTTATGGTATGCGCTGCTTGTGTGGCATGTGTGGCTATGCGGAACCGGACTTGCCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0005575 GO:0005576 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042335 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

160

Amino Acids

18.0

Weight (kDa)

9.14

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 61 - 153 2.2e-21 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50650
malus_domestica MD11G1211300.v1.1
prunus_persica Prupe.4G221300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041151
rosa_laevigata RLG00000034030
rosa_roxburghii Rroxscaffold_1G00039520
rosa_rugosa Rorug05G0190200 Rorug06G0014500
rosa_samantha Rh5BG280800 Rh5CG313000
rosa_wichuraiana Rw5G025960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
AccB1I GGYRCC 2 cut(s) 183, 414
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 2 cut(s) 262, 458
AclWI GGATC 2 cut(s) 236, 249
AcoI YGGCCR 2 cut(s) 158, 236
AcyI GRCGYC 1 cut(s) 288
AgsI TTSAA 1 cut(s) 20
AjiI CACGTC 1 cut(s) 126
AleI CACNNNNGTG 1 cut(s) 181
AluBI AGCT 1 cut(s) 294
AluI AGCT 1 cut(s) 294
Alw21I GWGCWC 1 cut(s) 71
AlwI GGATC 2 cut(s) 236, 249
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 5 cut(s) 158, 173, 190, 194, 236
ApeKI GCWGC 3 cut(s) 107, 110, 432
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 120
BaeGI GKGCMC 1 cut(s) 419
BalI TGGCCA 1 cut(s) 160
BamHI GGATCC 1 cut(s) 241
BanI GGYRCC 2 cut(s) 183, 414
BauI CACGAG 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 3 cut(s) 94, 97, 419
BccI CCATC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 268
BisI GCNGC 3 cut(s) 108, 111, 433
BlsI GCNGC 3 cut(s) 109, 112, 434
Bme1390I CCNGG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 5 cut(s) 185, 191, 243, 416, 462
BmrFI CCNGG 1 cut(s) 188
BmsI GCATC 2 cut(s) 128, 320
BpuMI CCSGG 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 5 cut(s) 76, 131, 170, 187, 197
BsaWI WCCGGW 2 cut(s) 226, 463
Bse118I RCCGGY 2 cut(s) 113, 192
Bse1I ACTGG 1 cut(s) 161
Bse3DI GCAATG 1 cut(s) 353
BseAI TCCGGA 1 cut(s) 226
BseDI CCNNGG 5 cut(s) 76, 131, 170, 187, 197
BseGI GGATG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 161
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 3 cut(s) 94, 97, 419
BshFI GGCC 5 cut(s) 160, 175, 192, 196, 238
BshNI GGYRCC 2 cut(s) 183, 414
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 6 cut(s) 114, 187, 193, 227, 239, 464
BsnI GGCC 5 cut(s) 160, 175, 192, 196, 238
Bsp1286I GDGCHC 2 cut(s) 71, 419
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 3 cut(s) 27, 241, 274
BspACI CCGC 2 cut(s) 262, 458
BspANI GGCC 5 cut(s) 160, 175, 192, 196, 238
BspEI TCCGGA 1 cut(s) 226
BspLI GGNNCC 5 cut(s) 185, 191, 243, 416, 462
BspPI GGATC 2 cut(s) 236, 249
BspT107I GGYRCC 2 cut(s) 183, 414
BsrDI GCAATG 1 cut(s) 353
BsrFI RCCGGY 2 cut(s) 113, 192
BsrI ACTGG 1 cut(s) 161
BssAI RCCGGY 2 cut(s) 113, 192
BssECI CCNNGG 5 cut(s) 76, 131, 170, 187, 197
BssMI GATC 3 cut(s) 27, 241, 274
BssNI GRCGYC 1 cut(s) 288
BssSI CACGAG 1 cut(s) 177
BssT1I CCWWGG 3 cut(s) 76, 170, 197
Bst2BI CACGAG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 132
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 194
BstDSI CCRYGG 1 cut(s) 131
BstF5I GGATG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 3 cut(s) 30, 244, 277
BstMBI GATC 3 cut(s) 27, 241, 274
BstMWI GCNNNNNNNGC 4 cut(s) 116, 259, 441, 450
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 268
BstSLI GKGCMC 1 cut(s) 419
BstV1I GCAGC 3 cut(s) 94, 97, 419
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 5 cut(s) 160, 175, 192, 196, 238
BtgI CCRYGG 1 cut(s) 131
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 194
CfoI GCGC 1 cut(s) 432
Cfr10I RCCGGY 2 cut(s) 113, 192
Cfr13I GGNCC 1 cut(s) 190
CviAII CATG 2 cut(s) 445, 477
CviJI RGCY 9 cut(s) 136, 160, 175, 192, 196, 238, 294, 384, 453
CviKI_1 RGCY 9 cut(s) 136, 160, 175, 192, 196, 238, 294, 384, 453
DpnI GATC 3 cut(s) 29, 243, 276
DpnII GATC 3 cut(s) 27, 241, 274
EaeI YGGCCR 2 cut(s) 158, 236
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco130I CCWWGG 3 cut(s) 76, 170, 197
EcoT14I CCWWGG 3 cut(s) 76, 170, 197
ErhI CCWWGG 3 cut(s) 76, 170, 197
FaeI CATG 2 cut(s) 448, 480
FaiI YATR 6 cut(s) 205, 423, 428, 446, 456, 478
FatI CATG 2 cut(s) 444, 476
Fnu4HI GCNGC 3 cut(s) 108, 111, 433
FokI GGATG 1 cut(s) 106
FseI GGCCGGCC 1 cut(s) 196
Fsp4HI GCNGC 3 cut(s) 108, 111, 433
GlaI GCGC 1 cut(s) 431
GluI GCNGC 3 cut(s) 108, 111, 433
HaeIII GGCC 5 cut(s) 160, 175, 192, 196, 238
HapII CCGG 6 cut(s) 114, 187, 193, 227, 239, 464
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 2 cut(s) 448, 480
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 324
HpaII CCGG 6 cut(s) 114, 187, 193, 227, 239, 464
HphI GGTGA 1 cut(s) 120
Hpy166II GTNNAC 2 cut(s) 286, 304
Hpy188I TCNGA 1 cut(s) 329
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 2 cut(s) 286, 304
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4IV ACGT 2 cut(s) 125, 288
HpyCH4V TGCA 2 cut(s) 119, 366
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 259, 441, 450
HpySE526I ACGT 2 cut(s) 125, 288
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 2 cut(s) 448, 480
HspAI GCGC 1 cut(s) 430
Kpn2I TCCGGA 1 cut(s) 226
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Kzo9I GATC 3 cut(s) 27, 241, 274
LpnPI CCDG 7 cut(s) 127, 142, 200, 206, 240, 252, 397
Lsp1109I GCAGC 3 cut(s) 94, 97, 419
LweI GCATC 2 cut(s) 128, 320
MaeII ACGT 2 cut(s) 125, 288
MaeIII GTNAC 2 cut(s) 126, 145
MalI GATC 3 cut(s) 29, 243, 276
MboI GATC 3 cut(s) 27, 241, 274
MboII GAAGA 1 cut(s) 230
MfeI CAATTG 1 cut(s) 307
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 2 cut(s) 71, 419
MlsI TGGCCA 1 cut(s) 160
MluCI AATT 3 cut(s) 20, 307, 391
MluNI TGGCCA 1 cut(s) 160
MnlI CCTC 1 cut(s) 173
Mox20I TGGCCA 1 cut(s) 160
MroI TCCGGA 1 cut(s) 226
MroNI GCCGGC 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 222
MscI TGGCCA 1 cut(s) 160
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 181
Msp20I TGGCCA 1 cut(s) 160
MspI CCGG 6 cut(s) 114, 187, 193, 227, 239, 464
MspR9I CCNGG 1 cut(s) 188
MunI CAATTG 1 cut(s) 307
MwoI GCNNNNNNNGC 4 cut(s) 116, 259, 441, 450
NaeI GCCGGC 1 cut(s) 194
NciI CCSGG 1 cut(s) 188
NdeII GATC 3 cut(s) 27, 241, 274
NgoMIV GCCGGC 1 cut(s) 192
NlaIII CATG 2 cut(s) 448, 480
NlaIV GGNNCC 5 cut(s) 185, 191, 243, 416, 462
NmuCI GTSAC 1 cut(s) 126
NspI RCATGY 1 cut(s) 448
OliI CACNNNNGTG 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdiI GCCGGC 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 1 cut(s) 324
PkrI GCNGC 3 cut(s) 109, 112, 434
PspN4I GGNNCC 5 cut(s) 185, 191, 243, 416, 462
PspPI GGNCC 1 cut(s) 190
PsuI RGATCY 1 cut(s) 241
RigI GGCCGGCC 1 cut(s) 196
RseI CAYNNNNRTG 1 cut(s) 181
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 3 cut(s) 108, 111, 433
Sau3AI GATC 3 cut(s) 27, 241, 274
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 2 cut(s) 71, 419
SetI ASST 4 cut(s) 128, 291, 296, 416
SfaNI GCATC 2 cut(s) 128, 320
SfcI CTRYAG 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 181
Sse9I AATT 3 cut(s) 20, 307, 391
SsiI CCGC 2 cut(s) 262, 458
SspI AATATT 1 cut(s) 379
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 3 cut(s) 76, 170, 197
TaaI ACNGT 1 cut(s) 132
TaiI ACGT 2 cut(s) 128, 291
TaqI TCGA 1 cut(s) 297
TasI AATT 3 cut(s) 20, 307, 391
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TseFI GTSAC 1 cut(s) 126
TseI GCWGC 3 cut(s) 107, 110, 432
Tsp45I GTSAC 1 cut(s) 126
TspGWI ACGGA 1 cut(s) 234
XceI RCATGY 1 cut(s) 448
XmnI GAANNNNTTC 1 cut(s) 222
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.