Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008918

Physical Location & Seq

Genomic Coordinates Forward (+)

18781764 .. 18784582

Gene Structure 2819 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G50700.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGGGGAATTGCTTAGCCAAGAAATACGGATTGGTGATGAAACCACAACAAAATGGTGAAAGATCAGTTGAGATCGAGAACAGAAGAAGGAGCACCCACCAAGATCCGTCTAAAATATCCACCGGAACGAATCAACCACCACCTTGGCGGAATCCAGCGAAACACAGTGGTGCTGCAGCTATACTCGAGAAGCCGTACGAAGACGTGAAACTGTTCTACACTTTGAGCAAAGAATTGGGTCGAGGCCAGTTTGGAGTAACGTATCTGTGCACAGAGAAGTCGACGGGGAAGAGATTCGCTTGCAAGTCGATTTCCAAGAAGAAACTTGTGACGAAAGGTGATAAAGAAGATATGAGGAGAGAGATTCAGATTATGCAGCATTTGAGTGGACAACCTAACATTGTTGAGTTTAAAGGAGCTTATGAAGATGAGAAAGCTGTGAATTTGGTTATGGAGCTTTGTGCTGGTGGTGAATTGTTCGATAGGATTTTAGCAAAGGGACATTACAGTGAGAGAGCAGCTGCTTCTGTTTGTAGACAGATTGTTAATGTTGTCAATATTTGTCATTTCATGGGTGTGATGCATAGAGACTTAAAGCCTGAGAATTTCTTGTTATCTAGCAAAGATGAGAAGGCTCTTATCAAAGCCACTGATTTCGGATTATCAGTCTTTATCGAAGAAGGGAGAGTGTATAAAGATATAGTTGGGAGTGCATATTATGTTGCTCCTGAAGTTTTGAAGCGGAGATATGGTAAAGAAATCGATATATGGAGTGCTGGAATTATACTATACATTCTACTTAGTGGTGTGCCTCCGTTTTGGGCTGAAACGGAGAAAGGGATATTTGATGCTATATTGGAAGGTGAAATCGACTTTGAATCCCAACCTTGGCCCTCAATTTCCAATAGTGCTAAAGATTTGGTACGTAGAATGTTGACACAAGATCCGAAAAGACGGATTTCTGCTGCTGAAGTTCTTAAGCATCCATGGCTAAGAGAAGGTGGAGAAGCATCGGATAAGCCTATTGATAGTGCTGTTCTCTCAAGGATGAAGCAATTTAGAGCGATGAATAAACTAAAGAAACTTGCTTTAAAGGTCATTGCGGAGAACATTGACACGGAAGAAATACAAGGCTTAAAGGCGATGTTTGCTAATATAGATACAGACAATAGTGGTACTATCACATACGAAGAATTGAAAGAAGGATTAGCCAAACTGGGATCAAGACTCACTGAAGCTGAAGTGAAACAGCTCATGGATGCTGCTGACGTTGATGGAAATGGGTCAATAGATTACATTGAGTTTATTACTGCAACAATGCATAGACATAGGCTTGAAAGTAATGAGAACGTTTACAAAGCCTTCCAACATTTTGACAAAGATGGGAGTGGATACATTACGACAGACGAGCTAGAGGCTGCTTTGAAGGAATATGGAATGGGAGATGATGCTACTATCAAAGAGATTTTGTCTGATGTCGACGCTGATAATGACGGTAGAATCAACTATGACGAGTTTTGTGCTATGATGAGAAGTGGGAATCCACAACAACCAAGATTGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

521

Amino Acids

58.61

Weight (kDa)

6.04

Isoelectric Point (pI)

41.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	73 - 331	1.2e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	74 - 326	3.6e-30	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	378 - 465	9.4e-09	EF-hand domain pair
EF-hand_1	PF00036	379 - 406	4.4e-07	EF hand domain
EF-hand_7	PF13499	476 - 537	7.4e-17	EF-hand domain pair
EF-hand_1	PF00036	478 - 503	3.1e-07	EF hand domain
EF-hand_6	PF13405	478 - 505	6.1e-08	EF-hand domain
EF-hand_1	PF00036	512 - 538	9.6e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008918)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50700 AT1G50700 AT1G50700 AT3G20410 AT3G20410
fragaria_vesca	FvH4_3g22730 FvH4_3g22730
malus_domestica	MD03G1200500.v1.1 MD11G1217500.v1.1
prunus_persica	Prupe.4G213800_v2.0.a1 Prupe.4G213800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0039651
rosa_laevigata	RLG00000033934
rosa_multiflora	Rmu_ssc0000400.1_g000007 Rmu_ssc0000400.1_g000008
rosa_roxburghii	Rroxscaffold_1G00041560
rosa_rugosa	Rorug05G0180900
rosa_samantha	Rh5AG267700 Rh5BG272000 Rh5CG304600 Rh5DG279700
rosa_wichuraiana	Rw5G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	281, 535, 1479
AciI	CCGC	3 cut(s)	148, 742, 1103
AclI	AACGTT	1 cut(s)	1350
AclWI	GGATC	3 cut(s)	98, 938, 1228
AcsI	RAATTY	2 cut(s)	442, 604
AcuI	CTGAAG	4 cut(s)	750, 990, 1254, 1260
AfaI	GTAC	3 cut(s)	197, 924, 1177
AfiI	CCNNNNNNNGG	1 cut(s)	888
AflII	CTTAAG	1 cut(s)	977
AgsI	TTSAA	5 cut(s)	739, 878, 1198, 1337, 1426
AjiI	CACGTC	1 cut(s)	205
AjuI	GAANNNNNNNTTGG	2 cut(s)	14, 46
AleI	CACNNNNGTG	1 cut(s)	168
AluBI	AGCT	8 cut(s)	179, 419, 437, 457, 521, 1238, 1252, 1411
AluI	AGCT	8 cut(s)	179, 419, 437, 457, 521, 1238, 1252, 1411
Alw21I	GWGCWC	2 cut(s)	95, 272
Alw26I	GTCTC	1 cut(s)	582
Alw44I	GTGCAC	1 cut(s)	268
AlwI	GGATC	3 cut(s)	98, 938, 1228
Ama87I	CYCGRG	1 cut(s)	185
AoxI	GGCC	2 cut(s)	244, 890
ApaLI	GTGCAC	1 cut(s)	268
ApeKI	GCWGC	8 cut(s)	173, 176, 376, 518, 521, 965, 1262, 1418
ApoI	RAATTY	2 cut(s)	442, 604
Asp700I	GAANNNNTTC	2 cut(s)	212, 293
AspS9I	GGNCC	1 cut(s)	891
AsuHPI	GGTGA	5 cut(s)	46, 68, 350, 482, 875
AvaI	CYCGRG	1 cut(s)	185
BaeGI	GKGCMC	1 cut(s)	272
BbsI	GAAGAC	1 cut(s)	207
Bbv12I	GWGCWC	2 cut(s)	95, 272
BbvI	GCAGC	8 cut(s)	160, 188, 388, 508, 530, 952, 1249, 1405
BccI	CCATC	2 cut(s)	1268, 1376
BceAI	ACGGC	1 cut(s)	178
BcgI	CGANNNNNNTGC	2 cut(s)	1502, 1536
BciVI	GTATCC	1 cut(s)	1385
BcoDI	GTCTC	1 cut(s)	582
BfaI	CTAG	2 cut(s)	618, 1412
BfmI	CTRYAG	1 cut(s)	174
BfrI	CTTAAG	1 cut(s)	977
BfuI	GTATCC	1 cut(s)	1385
BisI	GCNGC	8 cut(s)	174, 177, 377, 519, 522, 966, 1263, 1419
BlpI	GCTNAGC	1 cut(s)	13
BlsI	GCNGC	8 cut(s)	175, 178, 378, 520, 523, 967, 1264, 1420
BmeT110I	CYCGRG	1 cut(s)	185
BmgBI	CACGTC	1 cut(s)	205
BmgT120I	GGNCC	1 cut(s)	891
BmrI	ACTGGG	1 cut(s)	1226
BmsI	GCATC	6 cut(s)	570, 838, 991, 1019, 1249, 1438
BmuI	ACTGGG	1 cut(s)	1226
BpiI	GAAGAC	1 cut(s)	207
Bpu1102I	GCTNAGC	1 cut(s)	13
BpuEI	CTTGAG	1 cut(s)	1027
Bsa29I	ATCGAT	1 cut(s)	762
BsaAI	YACGTR	1 cut(s)	926
BsaJI	CCNNGG	3 cut(s)	143, 887, 986
BsaWI	WCCGGW	1 cut(s)	122
BsaXI	ACNNNNNCTCC	2 cut(s)	736, 766
Bsc4I	CCNNNNNNNGG	1 cut(s)	888
Bse1I	ACTGG	2 cut(s)	247, 1221
Bse3DI	GCAATG	1 cut(s)	1098
BseCI	ATCGAT	1 cut(s)	762
BseDI	CCNNGG	3 cut(s)	143, 887, 986
BseGI	GGATG	3 cut(s)	982, 1053, 1264
BseLI	CCNNNNNNNGG	1 cut(s)	888
BseMI	GCAATG	1 cut(s)	1098
BseMII	CTCAG	1 cut(s)	591
BseNI	ACTGG	2 cut(s)	247, 1221
BseRI	GAGGAG	1 cut(s)	370
BseSI	GKGCMC	1 cut(s)	272
BseXI	GCAGC	8 cut(s)	160, 188, 388, 508, 530, 952, 1249, 1405
BshFI	GGCC	2 cut(s)	246, 892
BshVI	ATCGAT	1 cut(s)	762
BsiHKAI	GWGCWC	2 cut(s)	95, 272
BsiHKCI	CYCGRG	1 cut(s)	185
BsiSI	CCGG	1 cut(s)	123
BsiWI	CGTACG	1 cut(s)	195
BslFI	GGGAC	1 cut(s)	513
BslI	CCNNNNNNNGG	1 cut(s)	888
BsmAI	GTCTC	1 cut(s)	582
BsmFI	GGGAC	1 cut(s)	513
BsnI	GGCC	2 cut(s)	246, 892
BsoBI	CYCGRG	1 cut(s)	185
Bsp1286I	GDGCHC	2 cut(s)	95, 272
Bsp143I	GATC	5 cut(s)	62, 72, 103, 943, 1220
Bsp1720I	GCTNAGC	1 cut(s)	13
Bsp19I	CCATGG	1 cut(s)	986
BspACI	CCGC	3 cut(s)	148, 742, 1103
BspANI	GGCC	2 cut(s)	246, 892
BspCNI	CTCAG	1 cut(s)	592
BspDI	ATCGAT	1 cut(s)	762
BspMAI	CTGCAG	1 cut(s)	178
BspPI	GGATC	3 cut(s)	98, 938, 1228
BspTI	CTTAAG	1 cut(s)	977
BsrDI	GCAATG	1 cut(s)	1098
BsrI	ACTGG	2 cut(s)	247, 1221
BssECI	CCNNGG	3 cut(s)	143, 887, 986
BssMI	GATC	5 cut(s)	62, 72, 103, 943, 1220
BssT1I	CCWWGG	3 cut(s)	143, 887, 986
Bst4CI	ACNGT	4 cut(s)	167, 213, 509, 1496
Bst6I	CTCTTC	1 cut(s)	284
BstAFI	CTTAAG	1 cut(s)	977
BstBAI	YACGTR	1 cut(s)	926
BstC8I	GCNNGC	1 cut(s)	301
BstDEI	CTNAG	4 cut(s)	13, 600, 800, 992
BstDSI	CCRYGG	1 cut(s)	986
BstF5I	GGATG	3 cut(s)	982, 1053, 1264
BstKTI	GATC	5 cut(s)	65, 75, 106, 946, 1223
BstMAI	GTCTC	1 cut(s)	582
BstMBI	GATC	5 cut(s)	62, 72, 103, 943, 1220
BstMWI	GCNNNNNNNGC	2 cut(s)	988, 1148
BstSFI	CTRYAG	1 cut(s)	174
BstSLI	GKGCMC	1 cut(s)	272
BstSNI	TACGTA	1 cut(s)	926
BstV1I	GCAGC	8 cut(s)	160, 188, 388, 508, 530, 952, 1249, 1405
BstV2I	GAAGAC	1 cut(s)	207
BstX2I	RGATCY	2 cut(s)	103, 943
BstXI	CCANNNNNNTGG	1 cut(s)	144
BstYI	RGATCY	2 cut(s)	103, 943
Bsu15I	ATCGAT	1 cut(s)	762
BsuI	GTATCC	1 cut(s)	1385
BsuRI	GGCC	2 cut(s)	246, 892
BsuTUI	ATCGAT	1 cut(s)	762
BtgI	CCRYGG	1 cut(s)	986
BtgZI	GCGATG	2 cut(s)	1079, 1157
BtrI	CACGTC	1 cut(s)	205
BtsCI	GGATG	3 cut(s)	982, 1053, 1264
BtsIMutI	CAGTG	4 cut(s)	172, 514, 648, 1230
Cac8I	GCNNGC	1 cut(s)	301
Cfr13I	GGNCC	1 cut(s)	891
ClaI	ATCGAT	1 cut(s)	762
CseI	GACGC	1 cut(s)	1490
Csp6I	GTAC	3 cut(s)	196, 923, 1176
CviAII	CATG	3 cut(s)	571, 987, 1255
CviQI	GTAC	3 cut(s)	196, 923, 1176
DdeI	CTNAG	4 cut(s)	13, 600, 800, 992
DpnI	GATC	5 cut(s)	64, 74, 105, 945, 1222
DpnII	GATC	5 cut(s)	62, 72, 103, 943, 1220
DraI	TTTAAA	2 cut(s)	412, 1092
Eam1104I	CTCTTC	1 cut(s)	284
EarI	CTCTTC	1 cut(s)	284
EciI	GGCGGA	1 cut(s)	163
Eco105I	TACGTA	1 cut(s)	926
Eco130I	CCWWGG	3 cut(s)	143, 887, 986
Eco57I	CTGAAG	4 cut(s)	750, 990, 1254, 1260
Eco88I	CYCGRG	1 cut(s)	185
EcoT14I	CCWWGG	3 cut(s)	143, 887, 986
EcoT22I	ATGCAT	2 cut(s)	585, 1323
ErhI	CCWWGG	3 cut(s)	143, 887, 986
FaeI	CATG	3 cut(s)	574, 990, 1258
FaqI	GGGAC	1 cut(s)	513
FatI	CATG	3 cut(s)	570, 986, 1254
FblI	GTMKAC	3 cut(s)	281, 535, 1479
Fnu4HI	GCNGC	8 cut(s)	174, 177, 377, 519, 522, 966, 1263, 1419
FokI	GGATG	3 cut(s)	969, 1060, 1271
Fsp4HI	GCNGC	8 cut(s)	174, 177, 377, 519, 522, 966, 1263, 1419
FspBI	CTAG	2 cut(s)	618, 1412
GluI	GCNGC	8 cut(s)	174, 177, 377, 519, 522, 966, 1263, 1419
HaeIII	GGCC	2 cut(s)	246, 892
HapII	CCGG	1 cut(s)	123
HgaI	GACGC	1 cut(s)	1490
Hin1II	CATG	3 cut(s)	574, 990, 1258
HincII	GTYRAC	3 cut(s)	282, 936, 1480
HindII	GTYRAC	3 cut(s)	282, 936, 1480
HinfI	GANTC	8 cut(s)	130, 151, 294, 364, 878, 1227, 1500, 1540
HpaII	CCGG	1 cut(s)	123
HphI	GGTGA	5 cut(s)	46, 68, 350, 482, 875
Hpy166II	GTNNAC	7 cut(s)	270, 282, 389, 536, 936, 1354, 1480
Hpy188I	TCNGA	5 cut(s)	369, 659, 948, 1015, 1474
Hpy188III	TCNNGA	4 cut(s)	76, 187, 728, 1224
Hpy8I	GTNNAC	7 cut(s)	270, 282, 389, 536, 936, 1354, 1480
Hpy99I	CGWCG	2 cut(s)	286, 1484
HpyAV	CCTTC	8 cut(s)	81, 625, 674, 854, 992, 1196, 1372, 1420
HpyCH4III	ACNGT	4 cut(s)	167, 213, 509, 1496
HpyCH4IV	ACGT	5 cut(s)	204, 260, 925, 1269, 1350
HpyCH4V	TGCA	8 cut(s)	176, 270, 303, 376, 583, 713, 1313, 1321
HpyF10VI	GCNNNNNNNGC	2 cut(s)	988, 1148
HpyF3I	CTNAG	4 cut(s)	13, 600, 800, 992
HpySE526I	ACGT	5 cut(s)	204, 260, 925, 1269, 1350
Hsp92II	CATG	3 cut(s)	574, 990, 1258
Kzo9I	GATC	5 cut(s)	62, 72, 103, 943, 1220
LmnI	GCTCC	4 cut(s)	90, 416, 454, 730
LpnPI	CCDG	8 cut(s)	136, 168, 260, 450, 612, 741, 762, 1202
Lsp1109I	GCAGC	8 cut(s)	160, 188, 388, 508, 530, 952, 1249, 1405
LweI	GCATC	6 cut(s)	570, 838, 991, 1019, 1249, 1438
MaeI	CTAG	2 cut(s)	618, 1412
MaeII	ACGT	5 cut(s)	204, 260, 925, 1269, 1350
MaeIII	GTNAC	2 cut(s)	256, 328
MalI	GATC	5 cut(s)	64, 74, 105, 945, 1222
MboI	GATC	5 cut(s)	62, 72, 103, 943, 1220
MboII	GAAGA	9 cut(s)	96, 212, 301, 331, 359, 437, 689, 1133, 1202
MflI	RGATCY	2 cut(s)	103, 943
MhlI	GDGCHC	2 cut(s)	95, 272
MluCI	AATT	9 cut(s)	7, 233, 442, 473, 604, 780, 897, 1055, 1193
MlyI	GAGTC	1 cut(s)	1221
MmeI	TCCRAC	1 cut(s)	1390
MnlI	CCTC	5 cut(s)	236, 348, 822, 904, 1408
Mph1103I	ATGCAT	2 cut(s)	585, 1323
MroXI	GAANNNNTTC	2 cut(s)	212, 293
MseI	TTAA	6 cut(s)	411, 546, 593, 978, 1091, 1136
MslI	CAYNNNNRTG	4 cut(s)	168, 384, 507, 575
MspA1I	CMGCKG	1 cut(s)	521
MspCI	CTTAAG	1 cut(s)	977
MspI	CCGG	1 cut(s)	123
MwoI	GCNNNNNNNGC	2 cut(s)	988, 1148
NcoI	CCATGG	1 cut(s)	986
NdeII	GATC	5 cut(s)	62, 72, 103, 943, 1220
NlaIII	CATG	3 cut(s)	574, 990, 1258
NmuCI	GTSAC	1 cut(s)	328
NsiI	ATGCAT	2 cut(s)	585, 1323
OliI	CACNNNNGTG	1 cut(s)	168
PaeR7I	CTCGAG	1 cut(s)	185
PdmI	GAANNNNTTC	2 cut(s)	212, 293
PfeI	GAWTC	7 cut(s)	130, 151, 294, 364, 878, 1500, 1540
Pfl23II	CGTACG	1 cut(s)	195
PkrI	GCNGC	8 cut(s)	175, 178, 378, 520, 523, 967, 1264, 1420
PleI	GAGTC	1 cut(s)	1221
PpsI	GAGTC	1 cut(s)	1221
Ppu21I	YACGTR	1 cut(s)	926
Psp1406I	AACGTT	1 cut(s)	1350
PspLI	CGTACG	1 cut(s)	195
PspPI	GGNCC	1 cut(s)	891
PstI	CTGCAG	1 cut(s)	178
PsuI	RGATCY	2 cut(s)	103, 943
PvuII	CAGCTG	1 cut(s)	521
RsaI	GTAC	3 cut(s)	197, 924, 1177
RsaNI	GTAC	3 cut(s)	196, 923, 1176
RseI	CAYNNNNRTG	4 cut(s)	168, 384, 507, 575
SalI	GTCGAC	2 cut(s)	280, 1478
SaqAI	TTAA	6 cut(s)	411, 546, 593, 978, 1091, 1136
SatI	GCNGC	8 cut(s)	174, 177, 377, 519, 522, 966, 1263, 1419
Sau3AI	GATC	5 cut(s)	62, 72, 103, 943, 1220
Sau96I	GGNCC	1 cut(s)	891
SchI	GAGTC	1 cut(s)	1221
SduI	GDGCHC	2 cut(s)	95, 272
SfaNI	GCATC	6 cut(s)	570, 838, 991, 1019, 1249, 1438
SfcI	CTRYAG	1 cut(s)	174
Sfr274I	CTCGAG	1 cut(s)	185
SlaI	CTCGAG	1 cut(s)	185
SmiMI	CAYNNNNRTG	4 cut(s)	168, 384, 507, 575
SmlI	CTYRAG	3 cut(s)	185, 977, 1042
SmoI	CTYRAG	3 cut(s)	185, 977, 1042
SnaBI	TACGTA	1 cut(s)	926
Sse9I	AATT	9 cut(s)	7, 233, 442, 473, 604, 780, 897, 1055, 1193
SsiI	CCGC	3 cut(s)	148, 742, 1103
SspI	AATATT	1 cut(s)	559
SspMI	CTAG	2 cut(s)	618, 1412
StyI	CCWWGG	3 cut(s)	143, 887, 986
TaaI	ACNGT	4 cut(s)	167, 213, 509, 1496
TaiI	ACGT	5 cut(s)	207, 263, 928, 1272, 1353
TasI	AATT	9 cut(s)	7, 233, 442, 473, 604, 780, 897, 1055, 1193
TfiI	GAWTC	7 cut(s)	130, 151, 294, 364, 878, 1500, 1540
Tru1I	TTAA	6 cut(s)	411, 546, 593, 978, 1091, 1136
Tru9I	TTAA	6 cut(s)	411, 546, 593, 978, 1091, 1136
TscAI	CASTG	4 cut(s)	172, 514, 655, 1237
TseFI	GTSAC	1 cut(s)	328
TseI	GCWGC	8 cut(s)	173, 176, 376, 518, 521, 965, 1262, 1418
Tsp45I	GTSAC	1 cut(s)	328
TspDTI	ATGAA	5 cut(s)	53, 438, 559, 1064, 1082
TspGWI	ACGGA	6 cut(s)	42, 96, 804, 845, 970, 1133
TspRI	CASTG	4 cut(s)	172, 514, 655, 1237
Vha464I	CTTAAG	1 cut(s)	977
VneI	GTGCAC	1 cut(s)	268
XapI	RAATTY	2 cut(s)	442, 604
XhoI	CTCGAG	1 cut(s)	185
XmiI	GTMKAC	3 cut(s)	281, 535, 1479
XmnI	GAANNNNTTC	2 cut(s)	212, 293
XspI	CTAG	2 cut(s)	618, 1412
Zsp2I	ATGCAT	2 cut(s)	585, 1323

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.