Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0008918

Physical Location & Seq

Genomic Coordinates Forward (+)

34358808 .. 34363177

Gene Structure 4370 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033934

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGGGCAAGTGTCTAAGCAAAAGCAGAGACCCAGAGCCACAACATAATGGGTACAGATCAGGAGGAGGAGCTGGACAACATTACCGAAAACCCCATGATCCAGTTGTTCACCAATCCAAGGCCTCTGTTCAGCAACATCATCAATTACCAGAGAAGCGCGTCATGGCAGCCCATCAAGAACCCCAACCAGCTTGGAAGCCATCTGTTCCAGCCAGAAACCCCAAACCAGTTCTCAGGCCAGACACAATTCTGGGGAAGCCATATGAGGATGTGAAGTTGCACTACACCTTGGGAAAAGAGTTGGGTAGAGGGCAATTTGGGGTCACATATCTTTGTACTGAGAATTCAAGTGGGAGGAAATATGCTTGCAAGTCAATCTCAAAGAGGAAGCTGGTGACCAAAAATGACAAGGAGGATATAAAGAGGGAGATTCAGATTATGCAGCATTTGAGTGGGCAGCCAAACATTGTGGAGTTCAAGGGGTCTTATGAGGACAAGCAGTCTGTGCATGTTTGTATGGAGCTTTGTGCAGGTGGTGAGCTTTTTGATAGGATTATTGCAAAGGGTCATTACAGTGAGAGAGCAGCTTCTGCAATATGCAGGTCAATTGTGAGTGTTGTGCATATCTGTCATTTCATGGGTGTGATGCATAGAGACCTCAAGCCTGAGAATTTCTTGCTGTCTAGCAAGGATGAGAATGCCCTTCTCAAGGCTACTGATTTCGGGTTGTCAGTGTTCATTGAAGAAGGAAAGGTGTATCGTGATATAGTTGGGAGTGCTTATTATGTTGCTCCTGAAGTACTACGGCGCAGATACGGCAAGGAAATAGATATCTGGAGTGCAGGAGTTATCCTGTATATTTTACTAAGTGGAGTACCTCCATTTTGGGCTGAATCAGAGAAGGGAATATTTGATGCCATATTGGAGGGAGTGATCGACTTTGATAGTGCACCGTGGCCATCAATATCACACAGTGCTAAGGACCTGGTCCGGAGGATGCTGACACAAGACCCTAAAAAGCGAATTACTTCAGCTCAGGTCTTAGAGCACCCGTGGCTCAAAGAAGAAGCATCAGACAAACCCATAGACAGTGCCGTGCTTTCTAGAATGAAGCAGTTTAGAGCAATGAACAAACTGAAAAAACTTGCATTGAAGGTTATTGCCGAAAATCTTTCTGAAGAAGAAATCCATGGGCTTAAGGCAATGTTTGCAAACATGGATACTGACAATAGTGGAACCATCACTTATGATGAATTGAAGTCAGGATTGGCTCGACTTGGCTCTAAGCTGACTGAGGCTGAAGTCAAGCAGCTAATGGAAGCTGCTGATGTGGATGGGAATGGAGCAATTGACTACATTGAATTCATTACTGCTACAATGCACAGACACAAGCTAGAGAGGGACGAACATCTTTACAAAGCCTTCCAATATTTTGACACAGACAACAGCGGGTTTATTACTAGAGATGAGCTAGAAAAAGCTATGAAAGAGTATGGTATGGGTGACGAAGCCACAATTAGGGATATAATATCTGAAGTAGATACAGATAATGATGGTAGGATCAACTATGAGGAGTTCTGCACGATGATGAGAAGTGGAACCCAACAGCAGGGCAAGCTATTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

542

Amino Acids

61.13

Weight (kDa)

6.72

Isoelectric Point (pI)

43.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	95 - 353	5.2e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	96 - 349	1.6e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	399 - 459	3.8e-13	EF-hand domain pair
EF-hand_1	PF00036	399 - 425	8.5e-07	EF hand domain
EF-hand_7	PF13499	469 - 531	3.3e-18	EF-hand domain pair
EF-hand_1	PF00036	471 - 497	4.6e-07	EF hand domain
EF-hand_6	PF13405	471 - 499	1.3e-07	EF-hand domain
EF-hand_8	PF13833	484 - 531	1.3e-08	EF-hand domain pair
EF-hand_1	PF00036	506 - 532	1.1e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008918)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50700 AT1G50700 AT1G50700 AT3G20410 AT3G20410
fragaria_vesca	FvH4_3g22730 FvH4_3g22730
malus_domestica	MD03G1200500.v1.1 MD11G1217500.v1.1
prunus_persica	Prupe.4G213800_v2.0.a1 Prupe.4G213800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0039651
rosa_laevigata	RLG00000033934
rosa_multiflora	Rmu_ssc0000400.1_g000007 Rmu_ssc0000400.1_g000008
rosa_roxburghii	Rroxscaffold_1G00041560
rosa_rugosa	Rorug05G0180900
rosa_samantha	Rh5AG267700 Rh5BG272000 Rh5CG304600 Rh5DG279700
rosa_wichuraiana	Rw5G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	521
Acc36I	ACCTGC	2 cut(s)	521, 591
AccII	CGCG	1 cut(s)	159
AccIII	TCCGGA	1 cut(s)	990
AciI	CCGC	1 cut(s)	1449
AclWI	GGATC	2 cut(s)	92, 1568
AcoI	YGGCCR	1 cut(s)	956
AcsI	RAATTY	3 cut(s)	343, 670, 1361
AcuI	CTGAAG	5 cut(s)	816, 1014, 1197, 1320, 1554
AdeI	CACNNNGTG	1 cut(s)	974
AfaI	GTAC	4 cut(s)	53, 337, 801, 876
AfiI	CCNNNNNNNGG	4 cut(s)	118, 709, 1518, 1609
AflII	CTTAAG	1 cut(s)	1196
AgsI	TTSAA	6 cut(s)	348, 478, 743, 1153, 1258, 1361
AhdI	GACNNNNNGTC	1 cut(s)	499
AjnI	CCWGG	1 cut(s)	984
AjuI	GAANNNNNNNTTGG	2 cut(s)	1250, 1282
Alw21I	GWGCWC	2 cut(s)	952, 1050
Alw26I	GTCTC	2 cut(s)	21, 648
Alw44I	GTGCAC	1 cut(s)	948
AlwI	GGATC	2 cut(s)	92, 1568
AlwNI	CAGNNNCTG	1 cut(s)	590
Aor13HI	TCCGGA	1 cut(s)	990
AoxI	GGCC	3 cut(s)	120, 236, 956
ApaLI	GTGCAC	1 cut(s)	948
ApeKI	GCWGC	6 cut(s)	167, 442, 457, 584, 1309, 1322
ApoI	RAATTY	3 cut(s)	343, 670, 1361
Asp700I	GAANNNNTTC	1 cut(s)	1027
AspLEI	GCGC	2 cut(s)	159, 810
AspS9I	GGNCC	2 cut(s)	982, 988
AsuHPI	GGTGA	4 cut(s)	101, 406, 548, 1514
AvaII	GGWCC	2 cut(s)	982, 988
BaeGI	GKGCMC	1 cut(s)	952
BalI	TGGCCA	1 cut(s)	958
BarI	GAAGNNNNNNTAC	2 cut(s)	266, 298
Bbv12I	GWGCWC	2 cut(s)	952, 1050
BbvI	GCAGC	6 cut(s)	179, 454, 469, 596, 1309, 1321
BccI	CCATC	6 cut(s)	180, 208, 967, 1247, 1328, 1547
BceAI	ACGGC	3 cut(s)	821, 832, 1079
BciT130I	CCWGG	1 cut(s)	986
BciVI	GTATCC	1 cut(s)	1213
BcoDI	GTCTC	2 cut(s)	21, 648
BfaI	CTAG	5 cut(s)	684, 1104, 1394, 1461, 1472
BfrI	CTTAAG	1 cut(s)	1196
BfuAI	ACCTGC	2 cut(s)	521, 591
BfuI	GTATCC	1 cut(s)	1213
BisI	GCNGC	6 cut(s)	168, 443, 458, 585, 1310, 1323
BlsI	GCNGC	6 cut(s)	169, 444, 459, 586, 1311, 1324
BmcAI	AGTACT	1 cut(s)	801
Bme1390I	CCNGG	1 cut(s)	986
Bme18I	GGWCC	2 cut(s)	982, 988
BmeRI	GACNNNNNGTC	1 cut(s)	499
BmgT120I	GGNCC	2 cut(s)	982, 988
BmiI	GGNNCC	2 cut(s)	1237, 1600
BmrFI	CCNGG	1 cut(s)	986
BmsI	GCATC	4 cut(s)	636, 904, 987, 1079
BpmI	CTGGAG	1 cut(s)	856
Bpu10I	CCTNAGC	2 cut(s)	978, 1035
BpuEI	CTTGAG	2 cut(s)	644, 692
BsaI	GGTCTC	2 cut(s)	21, 648
BsaJI	CCNNGG	5 cut(s)	117, 288, 953, 1052, 1189
BsaWI	WCCGGW	1 cut(s)	990
Bsc4I	CCNNNNNNNGG	4 cut(s)	118, 709, 1518, 1609
Bse1I	ACTGG	2 cut(s)	101, 227
Bse3DI	GCAATG	2 cut(s)	1131, 1209
BseAI	TCCGGA	1 cut(s)	990
BseBI	CCWGG	1 cut(s)	986
BseDI	CCNNGG	5 cut(s)	117, 288, 953, 1052, 1189
BseGI	GGATG	4 cut(s)	274, 697, 1002, 1339
BseLI	CCNNNNNNNGG	4 cut(s)	118, 709, 1518, 1609
BseMI	GCAATG	2 cut(s)	1131, 1209
BseMII	CTCAG	5 cut(s)	247, 330, 657, 1049, 1284
BseNI	ACTGG	2 cut(s)	101, 227
BseRI	GAGGAG	3 cut(s)	78, 81, 1586
BseSI	GKGCMC	1 cut(s)	952
BseXI	GCAGC	6 cut(s)	179, 454, 469, 596, 1309, 1321
BsgI	GTGCAG	3 cut(s)	549, 861, 1564
Bsh1236I	CGCG	1 cut(s)	159
BshFI	GGCC	3 cut(s)	122, 238, 958
BsiHKAI	GWGCWC	2 cut(s)	952, 1050
BsiSI	CCGG	1 cut(s)	991
BslFI	GGGAC	1 cut(s)	1415
BslI	CCNNNNNNNGG	4 cut(s)	118, 709, 1518, 1609
BsmAI	GTCTC	2 cut(s)	21, 648
BsmFI	GGGAC	1 cut(s)	1415
BsmI	GAATGC	1 cut(s)	703
BsnI	GGCC	3 cut(s)	122, 238, 958
Bso31I	GGTCTC	2 cut(s)	21, 648
Bsp1286I	GDGCHC	2 cut(s)	952, 1050
Bsp13I	TCCGGA	1 cut(s)	990
Bsp143I	GATC	4 cut(s)	56, 97, 933, 1560
Bsp19I	CCATGG	1 cut(s)	1189
BspACI	CCGC	1 cut(s)	1449
BspANI	GGCC	3 cut(s)	122, 238, 958
BspCNI	CTCAG	5 cut(s)	246, 331, 658, 1048, 1285
BspEI	TCCGGA	1 cut(s)	990
BspFNI	CGCG	1 cut(s)	159
BspLI	GGNNCC	2 cut(s)	1237, 1600
BspMI	ACCTGC	2 cut(s)	521, 591
BspPI	GGATC	2 cut(s)	92, 1568
BspTI	CTTAAG	1 cut(s)	1196
BspTNI	GGTCTC	2 cut(s)	21, 648
BsrDI	GCAATG	2 cut(s)	1131, 1209
BsrI	ACTGG	2 cut(s)	101, 227
BssECI	CCNNGG	5 cut(s)	117, 288, 953, 1052, 1189
BssMI	GATC	4 cut(s)	56, 97, 933, 1560
BssT1I	CCWWGG	3 cut(s)	117, 288, 1189
Bst2UI	CCWGG	1 cut(s)	986
Bst4CI	ACNGT	4 cut(s)	575, 954, 974, 1091
BstAFI	CTTAAG	1 cut(s)	1196
BstAPI	GCANNNNNTGC	3 cut(s)	505, 590, 1208
BstC8I	GCNNGC	2 cut(s)	367, 1616
BstDSI	CCRYGG	3 cut(s)	953, 1052, 1189
BstEII	GGTNACC	1 cut(s)	394
BstENI	CCTNNNNNAGG	1 cut(s)	707
BstF5I	GGATG	4 cut(s)	274, 697, 1002, 1339
BstFNI	CGCG	1 cut(s)	159
BstHHI	GCGC	2 cut(s)	159, 810
BstKTI	GATC	4 cut(s)	59, 100, 936, 1563
BstMAI	GTCTC	2 cut(s)	21, 648
BstMBI	GATC	4 cut(s)	56, 97, 933, 1560
BstMWI	GCNNNNNNNGC	6 cut(s)	505, 590, 816, 1054, 1208, 1615
BstNI	CCWGG	1 cut(s)	986
BstNSI	RCATGY	1 cut(s)	512
BstPI	GGTNACC	1 cut(s)	394
BstSCI	CCNGG	1 cut(s)	984
BstSLI	GKGCMC	1 cut(s)	952
BstUI	CGCG	1 cut(s)	159
BstV1I	GCAGC	6 cut(s)	179, 454, 469, 596, 1309, 1321
BsuI	GTATCC	1 cut(s)	1213
BsuRI	GGCC	3 cut(s)	122, 238, 958
BtgI	CCRYGG	3 cut(s)	953, 1052, 1189
BtsCI	GGATG	4 cut(s)	274, 697, 1002, 1339
BtsIMutI	CAGTG	4 cut(s)	580, 738, 979, 1096
BveI	ACCTGC	2 cut(s)	521, 591
Cac8I	GCNNGC	2 cut(s)	367, 1616
CaiI	CAGNNNCTG	1 cut(s)	590
CfoI	GCGC	2 cut(s)	159, 810
Cfr13I	GGNCC	2 cut(s)	982, 988
CseI	GACGC	1 cut(s)	148
CsiI	ACCWGGT	1 cut(s)	984
Csp6I	GTAC	4 cut(s)	52, 336, 800, 875
CspCI	CAANNNNNGTGG	2 cut(s)	450, 485
CviAII	CATG	6 cut(s)	95, 163, 509, 637, 1190, 1216
CviQI	GTAC	4 cut(s)	52, 336, 800, 875
DpnI	GATC	4 cut(s)	58, 99, 935, 1562
DpnII	GATC	4 cut(s)	56, 97, 933, 1560
DraIII	CACNNNGTG	1 cut(s)	974
DriI	GACNNNNNGTC	1 cut(s)	499
EaeI	YGGCCR	1 cut(s)	956
Eam1105I	GACNNNNNGTC	1 cut(s)	499
Eco130I	CCWWGG	3 cut(s)	117, 288, 1189
Eco147I	AGGCCT	1 cut(s)	122
Eco31I	GGTCTC	2 cut(s)	21, 648
Eco32I	GATATC	1 cut(s)	832
Eco47I	GGWCC	2 cut(s)	982, 988
Eco57I	CTGAAG	5 cut(s)	816, 1014, 1197, 1320, 1554
Eco91I	GGTNACC	1 cut(s)	394
EcoNI	CCTNNNNNAGG	1 cut(s)	707
EcoO109I	RGGNCCY	1 cut(s)	982
EcoO65I	GGTNACC	1 cut(s)	394
EcoRI	GAATTC	2 cut(s)	343, 1361
EcoRII	CCWGG	1 cut(s)	984
EcoRV	GATATC	1 cut(s)	832
EcoT14I	CCWWGG	3 cut(s)	117, 288, 1189
EcoT22I	ATGCAT	1 cut(s)	651
ErhI	CCWWGG	3 cut(s)	117, 288, 1189
FaeI	CATG	6 cut(s)	98, 166, 512, 640, 1193, 1219
FaqI	GGGAC	1 cut(s)	1415
FatI	CATG	6 cut(s)	94, 162, 508, 636, 1189, 1215
FauI	CCCGC	1 cut(s)	1442
FauNDI	CATATG	1 cut(s)	262
Fnu4HI	GCNGC	6 cut(s)	168, 443, 458, 585, 1310, 1323
FokI	GGATG	4 cut(s)	281, 704, 1009, 1346
Fsp4HI	GCNGC	6 cut(s)	168, 443, 458, 585, 1310, 1323
FspBI	CTAG	5 cut(s)	684, 1104, 1394, 1461, 1472
GlaI	GCGC	2 cut(s)	158, 809
GluI	GCNGC	6 cut(s)	168, 443, 458, 585, 1310, 1323
GsuI	CTGGAG	1 cut(s)	856
HaeIII	GGCC	3 cut(s)	122, 238, 958
HapII	CCGG	1 cut(s)	991
HgaI	GACGC	1 cut(s)	148
HhaI	GCGC	2 cut(s)	159, 810
Hin1II	CATG	6 cut(s)	98, 166, 512, 640, 1193, 1219
Hin6I	GCGC	2 cut(s)	157, 808
HinP1I	GCGC	2 cut(s)	157, 808
HinfI	GANTC	2 cut(s)	430, 893
HpaII	CCGG	1 cut(s)	991
HphI	GGTGA	4 cut(s)	101, 406, 548, 1514
Hpy166II	GTNNAC	2 cut(s)	109, 950
Hpy188I	TCNGA	5 cut(s)	435, 898, 1075, 1177, 1534
Hpy188III	TCNNGA	8 cut(s)	60, 176, 761, 794, 835, 991, 1104, 1263
Hpy8I	GTNNAC	2 cut(s)	109, 950
HpyAV	CCTTC	5 cut(s)	713, 740, 895, 1147, 1432
HpyCH4III	ACNGT	4 cut(s)	575, 954, 974, 1091
HpyF10VI	GCNNNNNNNGC	6 cut(s)	505, 590, 816, 1054, 1208, 1615
Hsp92II	CATG	6 cut(s)	98, 166, 512, 640, 1193, 1219
HspAI	GCGC	2 cut(s)	157, 808
Kpn2I	TCCGGA	1 cut(s)	990
Kzo9I	GATC	4 cut(s)	56, 97, 933, 1560
LmnI	GCTCC	4 cut(s)	68, 520, 796, 1343
Lsp1109I	GCAGC	6 cut(s)	179, 454, 469, 596, 1309, 1321
LweI	GCATC	4 cut(s)	636, 904, 987, 1079
MabI	ACCWGGT	1 cut(s)	984
MaeI	CTAG	5 cut(s)	684, 1104, 1394, 1461, 1472
MaeIII	GTNAC	3 cut(s)	322, 394, 1502
MalI	GATC	4 cut(s)	58, 99, 935, 1562
MboI	GATC	4 cut(s)	56, 97, 933, 1560
MboII	GAAGA	4 cut(s)	755, 1076, 1190, 1193
MfeI	CAATTG	2 cut(s)	606, 1347
MhlI	GDGCHC	2 cut(s)	952, 1050
MlsI	TGGCCA	1 cut(s)	958
MluNI	TGGCCA	1 cut(s)	958
Mox20I	TGGCCA	1 cut(s)	958
Mph1103I	ATGCAT	1 cut(s)	651
MroI	TCCGGA	1 cut(s)	990
MroXI	GAANNNNTTC	1 cut(s)	1027
MscI	TGGCCA	1 cut(s)	958
MseI	TTAA	1 cut(s)	1197
MslI	CAYNNNNRTG	4 cut(s)	450, 573, 641, 1586
Msp20I	TGGCCA	1 cut(s)	958
MspA1I	CMGCKG	1 cut(s)	1449
MspCI	CTTAAG	1 cut(s)	1196
MspI	CCGG	1 cut(s)	991
MspR9I	CCNGG	1 cut(s)	986
MunI	CAATTG	2 cut(s)	606, 1347
Mva1269I	GAATGC	1 cut(s)	703
MvaI	CCWGG	1 cut(s)	986
MvnI	CGCG	1 cut(s)	159
MwoI	GCNNNNNNNGC	6 cut(s)	505, 590, 816, 1054, 1208, 1615
NcoI	CCATGG	1 cut(s)	1189
NdeI	CATATG	1 cut(s)	262
NdeII	GATC	4 cut(s)	56, 97, 933, 1560
NlaIII	CATG	6 cut(s)	98, 166, 512, 640, 1193, 1219
NlaIV	GGNNCC	2 cut(s)	1237, 1600
NmuCI	GTSAC	3 cut(s)	322, 394, 1502
NsiI	ATGCAT	1 cut(s)	651
NspI	RCATGY	1 cut(s)	512
PaqCI	CACCTGC	1 cut(s)	521
PceI	AGGCCT	1 cut(s)	122
PctI	GAATGC	1 cut(s)	703
PdmI	GAANNNNTTC	1 cut(s)	1027
PfeI	GAWTC	2 cut(s)	430, 893
PflFI	GACNNNGTC	1 cut(s)	986
PkrI	GCNGC	6 cut(s)	169, 444, 459, 586, 1311, 1324
PpuMI	RGGWCCY	1 cut(s)	982
Psp5II	RGGWCCY	1 cut(s)	982
Psp6I	CCWGG	1 cut(s)	984
PspEI	GGTNACC	1 cut(s)	394
PspGI	CCWGG	1 cut(s)	984
PspN4I	GGNNCC	2 cut(s)	1237, 1600
PspPI	GGNCC	2 cut(s)	982, 988
PspPPI	RGGWCCY	1 cut(s)	982
PstNI	CAGNNNCTG	1 cut(s)	590
PsyI	GACNNNGTC	1 cut(s)	986
RsaI	GTAC	4 cut(s)	53, 337, 801, 876
RsaNI	GTAC	4 cut(s)	52, 336, 800, 875
RseI	CAYNNNNRTG	4 cut(s)	450, 573, 641, 1586
SaqAI	TTAA	1 cut(s)	1197
SatI	GCNGC	6 cut(s)	168, 443, 458, 585, 1310, 1323
Sau3AI	GATC	4 cut(s)	56, 97, 933, 1560
Sau96I	GGNCC	2 cut(s)	982, 988
ScaI	AGTACT	1 cut(s)	801
ScrFI	CCNGG	1 cut(s)	986
SduI	GDGCHC	2 cut(s)	952, 1050
SexAI	ACCWGGT	1 cut(s)	984
SfaNI	GCATC	4 cut(s)	636, 904, 987, 1079
SinI	GGWCC	2 cut(s)	982, 988
SmiMI	CAYNNNNRTG	4 cut(s)	450, 573, 641, 1586
SmlI	CTYRAG	3 cut(s)	659, 707, 1196
SmoI	CTYRAG	3 cut(s)	659, 707, 1196
SseBI	AGGCCT	1 cut(s)	122
SsiI	CCGC	1 cut(s)	1449
SspI	AATATT	2 cut(s)	909, 1430
SspMI	CTAG	5 cut(s)	684, 1104, 1394, 1461, 1472
StuI	AGGCCT	1 cut(s)	122
StyD4I	CCNGG	1 cut(s)	984
StyI	CCWWGG	3 cut(s)	117, 288, 1189
TaaI	ACNGT	4 cut(s)	575, 954, 974, 1091
TaqI	TCGA	2 cut(s)	936, 1273
TatI	WGTACW	2 cut(s)	335, 799
TfiI	GAWTC	2 cut(s)	430, 893
Tru1I	TTAA	1 cut(s)	1197
Tru9I	TTAA	1 cut(s)	1197
TscAI	CASTG	4 cut(s)	580, 738, 979, 1096
TseFI	GTSAC	3 cut(s)	322, 394, 1502
TseI	GCWGC	6 cut(s)	167, 442, 457, 584, 1309, 1322
Tsp45I	GTSAC	3 cut(s)	322, 394, 1502
TspDTI	ATGAA	7 cut(s)	625, 727, 1124, 1142, 1266, 1354, 1499
TspRI	CASTG	4 cut(s)	580, 738, 979, 1096
Tth111I	GACNNNGTC	1 cut(s)	986
Vha464I	CTTAAG	1 cut(s)	1196
VneI	GTGCAC	1 cut(s)	948
VpaK11BI	GGWCC	2 cut(s)	982, 988
XagI	CCTNNNNNAGG	1 cut(s)	707
XapI	RAATTY	3 cut(s)	343, 670, 1361
XbaI	TCTAGA	1 cut(s)	1103
XceI	RCATGY	1 cut(s)	512
XmnI	GAANNNNTTC	1 cut(s)	1027
XspI	CTAG	5 cut(s)	684, 1104, 1394, 1461, 1472
ZrmI	AGTACT	1 cut(s)	801
Zsp2I	ATGCAT	1 cut(s)	651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.