AT1G53163

MAP kinase kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015294
Physical Location & Seq
Forward (+)
19812087 .. 19813290
1204 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53163.1

Sequence Viewer

Length: 903 bp
ATGGAGGATAACCGGAAAACTACCCCAAACTCCGGCGAGACCAAACCAGAGACGTCCCTGGAGACGTCACTATCATCAGACTTCGAGTTTGGATCATTGACTCCCACATCTCCATCACAAGACCCCTTCTCGGAAAATTCTCCCGCTGATCATCTTTTCCTCAACGGCCGTCTATTACCACACTCTTTTCCAACGAGCACGATAACGACACGTTCAGTGTCACGTACTACCAGCGAAAACACTTTCCGGAGCGATAGTTGGAGCAGCCGAAGCAGTGATAGTAGCAGCCCGACATCTTACACTCCAAGAACAAGCACTTGTAATATCTTCAAGATCACAAGTTCTAGTACCGGTCATGGGAAAGAGACTAATATAACATGTTTTGGTACTCGGTCCGAAGAGATTATGTGCCTAAGGCCCGCAACTTCTTCAAAGAAAACAAACACTTGCATCAGCAAAGAATACTATCACAAACCATCGTTAATATTGGATCTAAAGAAGAGACCACCGGTGGAAAATGGTAAAGCCGTAAACACGACAAATAAGGTTTCGTATTTGCCGCAATGCAAGAGGAAGAAAGCGACGGAGATTGTAACGGCTCAGCTTTACGGGTCGTATTCACGGCGGTGGCAGCATATAACTCCGGTCTTTAAAAGAGAAGGGTCGGTGAAAAGTAAGGGAGGAGGGATTAAGGTCGGTGGACGGAAAAAGAAGTCGACGAGGAGGAAGAAGCCGAAGAAGGCGGAGGAGAGAAGAGGAATAAGTAGTAAGTTAATGAAGTGGTGGAAGAGAATTCTAAAGGCGGTGGTGTTTGCTTGCAGGGAGTGTCATGCAATGGAGCCTCATCAGATACTTGTAAATGATGATGCTGGTGTGAAAAAGAAGATTATTAAATGTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

33.59

Weight (kDa)

9.98

Isoelectric Point (pI)

62.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53163 AT3G15250
fragaria_vesca FvH4_3g19630
malus_domestica MD03G1230800.v1.1
prunus_persica Prupe.4G177000_v2.0.a1
pyrus_communis pycom11g22350
rosa_chinensis RchiOBHm_Chr5g0032821
rosa_laevigata RLG00000033431
rosa_multiflora Rmu_sc0000986.1_g000045
rosa_roxburghii Rroxscaffold_1G00047230
rosa_rugosa Rorug05G0134100
rosa_samantha Rh5AG226700 Rh5BG227800 Rh5CG255700 Rh5DG232600
rosa_wichuraiana Rw5G020800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 56, 68
AccI GTMKAC 1 cut(s) 716
AccIII TCCGGA 1 cut(s) 246
AciI CCGC 6 cut(s) 144, 420, 560, 625, 743, 803
AclWI GGATC 2 cut(s) 100, 498
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 2 cut(s) 136, 792
AcyI GRCGYC 2 cut(s) 53, 65
AfaI GTAC 3 cut(s) 226, 349, 388
AfiI CCNNNNNNNGG 3 cut(s) 32, 130, 357
AflIII ACRYGT 2 cut(s) 209, 377
AgeI ACCGGT 2 cut(s) 350, 508
AgsI TTSAA 2 cut(s) 331, 432
AjnI CCWGG 1 cut(s) 57
AleI CACNNNNGTG 1 cut(s) 625
AluBI AGCT 1 cut(s) 604
AluI AGCT 1 cut(s) 604
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 5 cut(s) 32, 44, 56, 359, 496
AlwI GGATC 2 cut(s) 100, 498
Aor13HI TCCGGA 1 cut(s) 246
AoxI GGCC 2 cut(s) 166, 416
ApeKI GCWGC 3 cut(s) 264, 285, 631
ApoI RAATTY 2 cut(s) 136, 792
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AsiGI ACCGGT 2 cut(s) 350, 508
AspS9I GGNCC 2 cut(s) 393, 417
AsuHPI GGTGA 1 cut(s) 679
AvaII GGWCC 1 cut(s) 393
AxyI CCTNAGG 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 3 cut(s) 276, 297, 643
BccI CCATC 2 cut(s) 121, 484
BceAI ACGGC 5 cut(s) 153, 181, 512, 612, 638
BciT130I CCWGG 1 cut(s) 59
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 5 cut(s) 32, 44, 56, 359, 496
BfaI CTAG 1 cut(s) 345
BisI GCNGC 4 cut(s) 265, 286, 560, 632
BlpI GCTNAGC 1 cut(s) 600
BlsI GCNGC 4 cut(s) 266, 287, 561, 633
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 393, 417
BmiI GGNNCC 1 cut(s) 840
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 2 cut(s) 459, 856
BpmI CTGGAG 1 cut(s) 80
Bpu1102I GCTNAGC 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 224
BsaHI GRCGYC 2 cut(s) 53, 65
BsaI GGTCTC 2 cut(s) 32, 496
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 5 cut(s) 12, 246, 350, 508, 643
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 130, 357
Bse118I RCCGGY 2 cut(s) 350, 508
Bse21I CCTNAGG 1 cut(s) 413
Bse3DI GCAATG 2 cut(s) 569, 840
BseAI TCCGGA 1 cut(s) 246
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 57
BseLI CCNNNNNNNGG 3 cut(s) 32, 130, 357
BseMI GCAATG 2 cut(s) 569, 840
BseMII CTCAG 1 cut(s) 614
BseRI GAGGAG 3 cut(s) 696, 736, 761
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 276, 297, 643
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 168, 418
BshTI ACCGGT 2 cut(s) 350, 508
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 200
BsiSI CCGG 6 cut(s) 13, 33, 247, 351, 509, 644
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 3 cut(s) 32, 130, 357
BsmAI GTCTC 5 cut(s) 32, 44, 56, 359, 496
BsmBI CGTCTC 2 cut(s) 44, 56
BsmFI GGGAC 1 cut(s) 40
BsnI GGCC 2 cut(s) 168, 418
Bso31I GGTCTC 2 cut(s) 32, 496
Bsp1286I GDGCHC 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 246
Bsp143I GATC 4 cut(s) 92, 148, 333, 490
Bsp1720I GCTNAGC 1 cut(s) 600
BspACI CCGC 6 cut(s) 144, 420, 560, 625, 743, 803
BspANI GGCC 2 cut(s) 168, 418
BspCNI CTCAG 1 cut(s) 613
BspEI TCCGGA 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 840
BspPI GGATC 2 cut(s) 100, 498
BspTNI GGTCTC 2 cut(s) 32, 496
BsrDI GCAATG 2 cut(s) 569, 840
BsrFI RCCGGY 2 cut(s) 350, 508
BssAI RCCGGY 2 cut(s) 350, 508
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 4 cut(s) 92, 148, 333, 490
BssNI GRCGYC 2 cut(s) 53, 65
Bst2UI CCWGG 1 cut(s) 59
Bst6I CTCTTC 4 cut(s) 393, 494, 748, 782
BstACI GRCGYC 2 cut(s) 53, 65
BstBAI YACGTR 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 420, 817
BstDEI CTNAG 2 cut(s) 413, 600
BstKTI GATC 4 cut(s) 95, 151, 336, 493
BstMAI GTCTC 5 cut(s) 32, 44, 56, 359, 496
BstMBI GATC 4 cut(s) 92, 148, 333, 490
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 270, 631
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 276, 297, 643
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BstZI CGGCCG 1 cut(s) 166
Bsu36I CCTNAGG 1 cut(s) 413
BsuRI GGCC 2 cut(s) 168, 418
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 222, 280
Cac8I GCNNGC 2 cut(s) 420, 817
Cfr10I RCCGGY 2 cut(s) 350, 508
Cfr13I GGNCC 2 cut(s) 393, 417
CpoI CGGWCCG 1 cut(s) 393
Csp6I GTAC 3 cut(s) 225, 348, 387
CspAI ACCGGT 2 cut(s) 350, 508
CspI CGGWCCG 1 cut(s) 393
CviAII CATG 3 cut(s) 356, 378, 830
CviJI RGCY 9 cut(s) 168, 267, 288, 418, 527, 599, 604, 733, 841
CviKI_1 RGCY 9 cut(s) 168, 267, 288, 418, 527, 599, 604, 733, 841
CviQI GTAC 3 cut(s) 225, 348, 387
DdeI CTNAG 2 cut(s) 413, 600
DpnI GATC 4 cut(s) 94, 150, 335, 492
DpnII GATC 4 cut(s) 92, 148, 333, 490
DraI TTTAAA 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 166
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 4 cut(s) 393, 494, 748, 782
EarI CTCTTC 4 cut(s) 393, 494, 748, 782
EciI GGCGGA 1 cut(s) 758
EclXI CGGCCG 1 cut(s) 166
Eco31I GGTCTC 2 cut(s) 32, 496
Eco47I GGWCC 1 cut(s) 393
Eco52I CGGCCG 1 cut(s) 166
Eco81I CCTNAGG 1 cut(s) 413
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 1 cut(s) 57
Esp3I CGTCTC 2 cut(s) 44, 56
FaeI CATG 3 cut(s) 359, 381, 833
FaiI YATR 8 cut(s) 357, 374, 379, 407, 636, 638, 831, 901
FaqI GGGAC 1 cut(s) 40
FatI CATG 3 cut(s) 355, 377, 829
FauI CCCGC 2 cut(s) 151, 427
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 4 cut(s) 265, 286, 560, 632
Fsp4HI GCNGC 4 cut(s) 265, 286, 560, 632
FspBI CTAG 1 cut(s) 345
GluI GCNGC 4 cut(s) 265, 286, 560, 632
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 2 cut(s) 168, 418
HapII CCGG 6 cut(s) 13, 33, 247, 351, 509, 644
Hin1I GRCGYC 2 cut(s) 53, 65
Hin1II CATG 3 cut(s) 359, 381, 833
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 1 cut(s) 100
HpaII CCGG 6 cut(s) 13, 33, 247, 351, 509, 644
HphI GGTGA 1 cut(s) 679
Hpy166II GTNNAC 3 cut(s) 532, 701, 717
Hpy188I TCNGA 4 cut(s) 79, 133, 397, 849
Hpy188III TCNNGA 2 cut(s) 247, 331
Hpy8I GTNNAC 3 cut(s) 532, 701, 717
Hpy99I CGWCG 2 cut(s) 586, 721
HpyAV CCTTC 3 cut(s) 136, 653, 733
HpyCH4IV ACGT 4 cut(s) 53, 65, 211, 223
HpyCH4V TGCA 4 cut(s) 450, 567, 819, 833
HpyF10VI GCNNNNNNNGC 2 cut(s) 270, 631
HpyF3I CTNAG 2 cut(s) 413, 600
HpySE526I ACGT 4 cut(s) 53, 65, 211, 223
Hsp92I GRCGYC 2 cut(s) 53, 65
Hsp92II CATG 3 cut(s) 359, 381, 833
Kpn2I TCCGGA 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 4 cut(s) 92, 148, 333, 490
LmnI GCTCC 3 cut(s) 249, 261, 838
Lsp1109I GCAGC 3 cut(s) 276, 297, 643
LweI GCATC 2 cut(s) 459, 856
MaeI CTAG 1 cut(s) 345
MaeII ACGT 4 cut(s) 53, 65, 211, 223
MaeIII GTNAC 3 cut(s) 66, 219, 592
MalI GATC 4 cut(s) 94, 150, 335, 492
MboI GATC 4 cut(s) 92, 148, 333, 490
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 2 cut(s) 136, 792
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 2 cut(s) 215, 239
MnlI CCTC 9 cut(s) 170, 564, 674, 677, 714, 717, 739, 749, 852
MroI TCCGGA 1 cut(s) 246
MseI TTAA 5 cut(s) 482, 651, 690, 773, 891
MslI CAYNNNNRTG 1 cut(s) 625
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 6 cut(s) 13, 33, 247, 351, 509, 644
MspR9I CCNGG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 2 cut(s) 270, 631
NdeII GATC 4 cut(s) 92, 148, 333, 490
NlaIII CATG 3 cut(s) 359, 381, 833
NlaIV GGNNCC 1 cut(s) 840
NmuCI GTSAC 2 cut(s) 66, 219
NspI RCATGY 1 cut(s) 381
OliI CACNNNNGTG 1 cut(s) 625
PciI ACATGT 1 cut(s) 377
PinAI ACCGGT 2 cut(s) 350, 508
PkrI GCNGC 4 cut(s) 266, 287, 561, 633
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
Ppu21I YACGTR 1 cut(s) 224
PscI ACATGT 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 1 cut(s) 840
PspPI GGNCC 2 cut(s) 393, 417
PsuI RGATCY 1 cut(s) 490
RsaI GTAC 3 cut(s) 226, 349, 388
RsaNI GTAC 3 cut(s) 225, 348, 387
RseI CAYNNNNRTG 1 cut(s) 625
Rsr2I CGGWCCG 1 cut(s) 393
RsrII CGGWCCG 1 cut(s) 393
SalI GTCGAC 1 cut(s) 715
SaqAI TTAA 5 cut(s) 482, 651, 690, 773, 891
SatI GCNGC 4 cut(s) 265, 286, 560, 632
Sau3AI GATC 4 cut(s) 92, 148, 333, 490
Sau96I GGNCC 2 cut(s) 393, 417
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 1 cut(s) 200
SetI ASST 7 cut(s) 56, 68, 214, 226, 549, 606, 696
SfaNI GCATC 2 cut(s) 459, 856
SgrAI CRCCGGYG 1 cut(s) 508
SinI GGWCC 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 625
Sse9I AATT 2 cut(s) 136, 792
SsiI CCGC 6 cut(s) 144, 420, 560, 625, 743, 803
SspI AATATT 1 cut(s) 486
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 57
TaiI ACGT 4 cut(s) 56, 68, 214, 226
TaqI TCGA 2 cut(s) 84, 716
TaqII GACCGA 1 cut(s) 381
TasI AATT 2 cut(s) 136, 792
TauI GCSGC 1 cut(s) 562
Tru1I TTAA 5 cut(s) 482, 651, 690, 773, 891
Tru9I TTAA 5 cut(s) 482, 651, 690, 773, 891
TscAI CASTG 2 cut(s) 222, 280
TseFI GTSAC 2 cut(s) 66, 219
TseI GCWGC 3 cut(s) 264, 285, 631
Tsp45I GTSAC 2 cut(s) 66, 219
TspDTI ATGAA 1 cut(s) 791
TspGWI ACGGA 2 cut(s) 599, 718
TspRI CASTG 2 cut(s) 222, 280
VpaK11BI GGWCC 1 cut(s) 393
XapI RAATTY 2 cut(s) 136, 792
XceI RCATGY 1 cut(s) 381
XmiI GTMKAC 1 cut(s) 716
XspI CTAG 1 cut(s) 345
ZraI GACGTC 2 cut(s) 54, 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.