FvH4_3g19630

MAP kinase kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015294
Physical Location & Seq
Reverse (-)
12701135 .. 12701878
744 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19630.t1

Sequence Viewer

Length: 744 bp
ATGGACAACCGCCGGAAAAAGAGCTCCTCCGGCGACACATTTTCCTTCCCGAGCACACCGGTCCACGAACAAGACTCTGAAGACTTCGAATTTGGCTGCTTCACACCCGACTCGGCCTCGAGTGACCCCTGCAAGGATTCCCCAGCTGACCATTTGTTCTTCAATGGTAAACTCCTACCTCATGCCTTCCCTTTTCAACCCAACAGCAATGGCATGCTACTTGTCGTTGATCACTCTCGTCGAACAAGCCGAACGAGCAGCATTAGCAGCAAGGACTCGCTTTCACTCATGTCATCAAGAAGCAACAGCACTAACAGTAGAAGCAGCACTAGCAGTTGCAGCAGCAGCGCAAGAACAAGTTCAAGTGACAATTCTGAAAGAAAGTTGATGTATCATCATCACTACAGCAGAACGACACCGTTTAGATCATCGGACAGGTACAAAACCGGAGGTGGTGCACAACTTTATGGTTCTTCTCAGAGATGGCAATTCATAACACCTGTGCCGACTTTAAGCCGTGACACTTCGGTTAAGAAGAAAGTGGAGATTGCTGCAGCTGGGAAAGGAAAAACCAACAAAGCAAAGAAGAAGAGGAAGGTCAAGGTTGTCCGGCGCCGGAGGCTGTGCCGGAGGCTTTTCCGGTGGCTGGTTTCGGCCTGTATAAAGTGTCACGCAATGGAGCCATCCAAGAAAAGGGATGTTGTTGATCATGTAAAAGATGGGAATCATGTGAAGCTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.75

Weight (kDa)

10.23

Isoelectric Point (pI)

73.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53163 AT3G15250
fragaria_vesca FvH4_3g19630
malus_domestica MD03G1230800.v1.1
prunus_persica Prupe.4G177000_v2.0.a1
pyrus_communis pycom11g22350
rosa_chinensis RchiOBHm_Chr5g0032821
rosa_laevigata RLG00000033431
rosa_multiflora Rmu_sc0000986.1_g000045
rosa_roxburghii Rroxscaffold_1G00047230
rosa_rugosa Rorug05G0134100
rosa_samantha Rh5AG226700 Rh5BG227800 Rh5CG255700 Rh5DG232600
rosa_wichuraiana Rw5G020800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 612
AciI CCGC 1 cut(s) 10
AcsI RAATTY 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 613
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 3 cut(s) 133, 646, 693
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 3 cut(s) 163, 197, 363
AluBI AGCT 4 cut(s) 24, 146, 557, 736
AluI AGCT 4 cut(s) 24, 146, 557, 736
Alw21I GWGCWC 3 cut(s) 26, 56, 460
Alw44I GTGCAC 1 cut(s) 456
Ama87I CYCGRG 2 cut(s) 49, 118
AoxI GGCC 2 cut(s) 114, 654
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 9 cut(s) 96, 258, 267, 324, 339, 342, 345, 551, 554
ApoI RAATTY 1 cut(s) 89
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AsiGI ACCGGT 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 358
AspLEI GCGC 2 cut(s) 350, 615
AspS9I GGNCC 1 cut(s) 61
AsuII TTCGAA 1 cut(s) 87
AvaI CYCGRG 2 cut(s) 49, 118
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 612
BanII GRGCYC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 3 cut(s) 26, 56, 460
BbvI GCAGC 9 cut(s) 83, 270, 279, 336, 351, 354, 357, 538, 566
BccI CCATC 3 cut(s) 477, 691, 713
BceAI ACGGC 1 cut(s) 501
BclI TGATCA 2 cut(s) 229, 706
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 2 cut(s) 403, 552
BfoI RGCGCY 1 cut(s) 616
BisI GCNGC 9 cut(s) 97, 259, 268, 325, 340, 343, 346, 552, 555
BlsI GCNGC 9 cut(s) 98, 260, 269, 326, 341, 344, 347, 553, 556
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 2 cut(s) 49, 118
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 2 cut(s) 614, 681
BpiI GAAGAC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 87
BsaBI GATNNNNATC 1 cut(s) 723
BsaHI GRCGYC 1 cut(s) 613
BsaWI WCCGGW 3 cut(s) 58, 446, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 646, 693
Bse118I RCCGGY 1 cut(s) 58
Bse3DI GCAATG 2 cut(s) 214, 681
Bse8I GATNNNNATC 1 cut(s) 723
BseGI GGATG 2 cut(s) 683, 703
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 133, 646, 693
BseMI GCAATG 2 cut(s) 214, 681
BseMII CTCAG 1 cut(s) 491
BseRI GAGGAG 1 cut(s) 16
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 9 cut(s) 83, 270, 279, 336, 351, 354, 357, 538, 566
BseYI CCCAGC 2 cut(s) 142, 557
BshFI GGCC 2 cut(s) 116, 656
BshNI GGYRCC 1 cut(s) 612
BshTI ACCGGT 1 cut(s) 58
BsiHKAI GWGCWC 3 cut(s) 26, 56, 460
BsiHKCI CYCGRG 2 cut(s) 49, 118
BsiSI CCGG 8 cut(s) 13, 30, 59, 447, 610, 616, 628, 640
BslI CCNNNNNNNGG 3 cut(s) 133, 646, 693
BsnI GGCC 2 cut(s) 116, 656
BsoBI CYCGRG 2 cut(s) 49, 118
Bsp119I TTCGAA 1 cut(s) 87
Bsp1286I GDGCHC 3 cut(s) 26, 56, 460
Bsp143I GATC 3 cut(s) 229, 425, 706
BspACI CCGC 1 cut(s) 10
BspANI GGCC 2 cut(s) 116, 656
BspCNI CTCAG 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 614, 681
BspMAI CTGCAG 1 cut(s) 556
BspT104I TTCGAA 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 612
BsrDI GCAATG 2 cut(s) 214, 681
BsrFI RCCGGY 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 58
BssMI GATC 3 cut(s) 229, 425, 706
BssNI GRCGYC 1 cut(s) 613
Bst4CI ACNGT 2 cut(s) 317, 420
Bst6I CTCTTC 1 cut(s) 584
BstACI GRCGYC 1 cut(s) 613
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 477
BstF5I GGATG 2 cut(s) 683, 703
BstH2I RGCGCY 1 cut(s) 616
BstHHI GCGC 2 cut(s) 350, 615
BstKTI GATC 3 cut(s) 232, 428, 709
BstMBI GATC 3 cut(s) 229, 425, 706
BstMWI GCNNNNNNNGC 8 cut(s) 30, 255, 264, 267, 330, 339, 345, 619
BstNSI RCATGY 1 cut(s) 217
BstSFI CTRYAG 2 cut(s) 403, 552
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 9 cut(s) 83, 270, 279, 336, 351, 354, 357, 538, 566
BstV2I GAAGAC 1 cut(s) 87
BsuRI GGCC 2 cut(s) 116, 656
BtsCI GGATG 2 cut(s) 683, 703
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 2 cut(s) 350, 615
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 1 cut(s) 439
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 5 cut(s) 182, 214, 289, 710, 728
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 1 cut(s) 477
DinI GGCGCC 1 cut(s) 614
DpnI GATC 3 cut(s) 231, 427, 708
DpnII GATC 3 cut(s) 229, 425, 706
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 49, 118
EcoICRI GAGCTC 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 26
EgeI GGCGCC 1 cut(s) 614
EheI GGCGCC 1 cut(s) 614
FaeI CATG 5 cut(s) 185, 217, 292, 713, 731
FaiI YATR 8 cut(s) 183, 215, 290, 468, 494, 662, 711, 729
FatI CATG 5 cut(s) 181, 213, 288, 709, 727
FbaI TGATCA 2 cut(s) 229, 706
Fnu4HI GCNGC 9 cut(s) 97, 259, 268, 325, 340, 343, 346, 552, 555
FokI GGATG 2 cut(s) 670, 710
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 9 cut(s) 97, 259, 268, 325, 340, 343, 346, 552, 555
FspBI CTAG 1 cut(s) 330
GlaI GCGC 2 cut(s) 349, 614
GluI GCNGC 9 cut(s) 97, 259, 268, 325, 340, 343, 346, 552, 555
GsaI CCCAGC 2 cut(s) 146, 561
HaeII RGCGCY 1 cut(s) 616
HaeIII GGCC 2 cut(s) 116, 656
HapII CCGG 8 cut(s) 13, 30, 59, 447, 610, 616, 628, 640
HhaI GCGC 2 cut(s) 350, 615
Hin1I GRCGYC 1 cut(s) 613
Hin1II CATG 5 cut(s) 185, 217, 292, 713, 731
Hin6I GCGC 2 cut(s) 348, 613
HinP1I GCGC 2 cut(s) 348, 613
HinfI GANTC 5 cut(s) 74, 110, 137, 275, 724
HpaII CCGG 8 cut(s) 13, 30, 59, 447, 610, 616, 628, 640
Hpy166II GTNNAC 3 cut(s) 64, 170, 458
Hpy188I TCNGA 4 cut(s) 79, 376, 433, 480
Hpy188III TCNNGA 2 cut(s) 49, 297
Hpy8I GTNNAC 3 cut(s) 64, 170, 458
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 3 cut(s) 55, 196, 589
HpyCH4III ACNGT 2 cut(s) 317, 420
HpyCH4V TGCA 4 cut(s) 132, 339, 458, 554
HpyF10VI GCNNNNNNNGC 8 cut(s) 30, 255, 264, 267, 330, 339, 345, 619
HpyF3I CTNAG 1 cut(s) 477
Hsp92I GRCGYC 1 cut(s) 613
Hsp92II CATG 5 cut(s) 185, 217, 292, 713, 731
HspAI GCGC 2 cut(s) 348, 613
KasI GGCGCC 1 cut(s) 612
Ksp22I TGATCA 2 cut(s) 229, 706
Kzo9I GATC 3 cut(s) 229, 425, 706
LmnI GCTCC 2 cut(s) 29, 679
Lsp1109I GCAGC 9 cut(s) 83, 270, 279, 336, 351, 354, 357, 538, 566
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 4 cut(s) 122, 365, 518, 668
MalI GATC 3 cut(s) 231, 427, 708
MboI GATC 3 cut(s) 229, 425, 706
MboII GAAGA 6 cut(s) 92, 151, 465, 547, 598, 601
MhlI GDGCHC 3 cut(s) 26, 56, 460
MluCI AATT 3 cut(s) 89, 370, 488
Mly113I GGCGCC 1 cut(s) 613
MlyI GAGTC 3 cut(s) 68, 104, 269
MnlI CCTC 7 cut(s) 37, 127, 189, 443, 585, 612, 624
MroXI GAANNNNTTC 1 cut(s) 358
MseI TTAA 2 cut(s) 512, 531
MspA1I CMGCKG 2 cut(s) 146, 557
MspI CCGG 8 cut(s) 13, 30, 59, 447, 610, 616, 628, 640
MwoI GCNNNNNNNGC 8 cut(s) 30, 255, 264, 267, 330, 339, 345, 619
NarI GGCGCC 1 cut(s) 613
NdeII GATC 3 cut(s) 229, 425, 706
NlaIII CATG 5 cut(s) 185, 217, 292, 713, 731
NlaIV GGNNCC 2 cut(s) 614, 681
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 4 cut(s) 122, 365, 518, 668
NspI RCATGY 1 cut(s) 217
NspV TTCGAA 1 cut(s) 87
PaeI GCATGC 1 cut(s) 217
PaeR7I CTCGAG 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 247
PdmI GAANNNNTTC 1 cut(s) 358
PfeI GAWTC 2 cut(s) 137, 724
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 9 cut(s) 98, 260, 269, 326, 341, 344, 347, 553, 556
PleI GAGTC 3 cut(s) 68, 104, 269
PluTI GGCGCC 1 cut(s) 616
PpsI GAGTC 3 cut(s) 68, 104, 269
Psp124BI GAGCTC 1 cut(s) 26
PspFI CCCAGC 2 cut(s) 142, 557
PspN4I GGNNCC 2 cut(s) 614, 681
PspPI GGNCC 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 118
PstI CTGCAG 1 cut(s) 556
PvuII CAGCTG 2 cut(s) 146, 557
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 512, 531
SatI GCNGC 9 cut(s) 97, 259, 268, 325, 340, 343, 346, 552, 555
Sau3AI GATC 3 cut(s) 229, 425, 706
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 3 cut(s) 68, 104, 269
SduI GDGCHC 3 cut(s) 26, 56, 460
SfcI CTRYAG 2 cut(s) 403, 552
SfoI GGCGCC 1 cut(s) 614
Sfr274I CTCGAG 1 cut(s) 118
SfuI TTCGAA 1 cut(s) 87
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 1 cut(s) 118
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
SphI GCATGC 1 cut(s) 217
Sse9I AATT 3 cut(s) 89, 370, 488
SsiI CCGC 1 cut(s) 10
SspDI GGCGCC 1 cut(s) 612
SspMI CTAG 1 cut(s) 330
SstI GAGCTC 1 cut(s) 26
TaaI ACNGT 2 cut(s) 317, 420
TaqI TCGA 3 cut(s) 87, 119, 241
TasI AATT 3 cut(s) 89, 370, 488
TfiI GAWTC 2 cut(s) 137, 724
Tru1I TTAA 2 cut(s) 512, 531
Tru9I TTAA 2 cut(s) 512, 531
TseFI GTSAC 4 cut(s) 122, 365, 518, 668
TseI GCWGC 9 cut(s) 96, 258, 267, 324, 339, 342, 345, 551, 554
Tsp45I GTSAC 4 cut(s) 122, 365, 518, 668
TspDTI ATGAA 1 cut(s) 481
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 217
XhoI CTCGAG 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 358
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.