pycom11g22350

MAP kinase kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0015294
Physical Location & Seq
Forward (+)
25344878 .. 25345654
777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g22350.1

Sequence Viewer

Length: 777 bp
ATGCAAATACACCAGCCTCCCCCTCAAAAAAACCAACCATCCATGGAAAATCGAAGAAAAAATAGCTCCTCCGGCGACACATTCTCCTTCCCTAGCACTCCGGTCCATGACCAAGACTCCGACTTCGAGTTTGGGTGCATCACCCCGGACTCAGCCTCCAGCACCGATCCCTGCAAAGACTCCCCCGCAGACCATTTGTTCCTCAATGGGAGGCTCTTGCCACATGCCTTCCCATTTCAACCTATTAGCAACGGCTTTATGGTTGTCGATAATAGTTCTCGCAGAACAAGCCGGACAAGCAGCATTAGCAGCAAGGACTCTCTCATGTCGTCGAGAAGCAATAGCACCAACAGTAGGAGTAGCAGTTGTAGCAGCAGTGCTAGGACAAGCTCAAGTGATAACTCTGAGAGAAAGTTGTATCACTCCAAATTATCATCCGATAGACACAAAGGCAACAAACCTAATAACAATATTTCAAGTCATCAAGTTTATGGGAATTCTCAGAGGTGGCAATTCATAACGGCCGTGCCCGTGCCAGCTTCGTTAAGCCGTGATAATTCTCGGAAGAAAGTGATCGAACTGGATCAGCTTGAAAGGAAAGAGAAATCCGTTAAAGCGAAGAAAAAAAGGCAGGTGAAGACGGTGGTGTCCCGGCGGAGATTTTTATTTGGCCGGAGGTTTTTCCGGTGGCTTGTTTCGACTTGCAAAAAGTGTCACGCAATGGAGCCATCCAAGAAAAGTACTAATGATAATGTAATTAATCTGGAAATTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.12

Weight (kDa)

10.19

Isoelectric Point (pI)

72.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53163 AT3G15250
fragaria_vesca FvH4_3g19630
malus_domestica MD03G1230800.v1.1
prunus_persica Prupe.4G177000_v2.0.a1
pyrus_communis pycom11g22350
rosa_chinensis RchiOBHm_Chr5g0032821
rosa_laevigata RLG00000033431
rosa_multiflora Rmu_sc0000986.1_g000045
rosa_roxburghii Rroxscaffold_1G00047230
rosa_rugosa Rorug05G0134100
rosa_samantha Rh5AG226700 Rh5BG227800 Rh5CG255700 Rh5DG232600
rosa_wichuraiana Rw5G020800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 622
AasI GACNNNNNNGTC 1 cut(s) 646
Acc36I ACCTGC 1 cut(s) 622
AciI CCGC 2 cut(s) 186, 655
AclWI GGATC 2 cut(s) 161, 591
AcoI YGGCCR 2 cut(s) 522, 670
AcsI RAATTY 1 cut(s) 496
AfaI GTAC 1 cut(s) 742
AfiI CCNNNNNNNGG 1 cut(s) 354
AgsI TTSAA 3 cut(s) 239, 477, 593
AluBI AGCT 5 cut(s) 66, 390, 539, 589, 774
AluI AGCT 5 cut(s) 66, 390, 539, 589, 774
AlwI GGATC 2 cut(s) 161, 591
AoxI GGCC 2 cut(s) 522, 670
ApeKI GCWGC 3 cut(s) 300, 309, 372
ApoI RAATTY 1 cut(s) 496
ArsI GACNNNNNNTTYG 4 cut(s) 107, 113, 139, 145
AseI ATTAAT 1 cut(s) 759
AspS9I GGNCC 1 cut(s) 103
AsuC2I CCSGG 2 cut(s) 146, 652
AsuHPI GGTGA 2 cut(s) 133, 646
AvaII GGWCC 1 cut(s) 103
BaeGI GKGCMC 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 644
BbvI GCAGC 3 cut(s) 312, 321, 384
BccI CCATC 2 cut(s) 46, 736
BceAI ACGGC 4 cut(s) 268, 509, 534, 537
BcnI CCSGG 2 cut(s) 146, 652
BfaI CTAG 2 cut(s) 93, 381
BfuAI ACCTGC 1 cut(s) 622
BisI GCNGC 3 cut(s) 301, 310, 373
BlsI GCNGC 3 cut(s) 302, 311, 374
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 2 cut(s) 146, 652
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 2 cut(s) 146, 652
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 376
BpuMI CCSGG 2 cut(s) 146, 652
BsaJI CCNNGG 2 cut(s) 42, 144
BsaWI WCCGGW 2 cut(s) 100, 684
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 101, 131, 140, 170, 349, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse1I ACTGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 726
BseDI CCNNGG 2 cut(s) 42, 144
BseGI GGATG 3 cut(s) 38, 434, 728
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 3 cut(s) 165, 396, 515
BseNI ACTGG 1 cut(s) 585
BseRI GAGGAG 1 cut(s) 58
BseSI GKGCMC 1 cut(s) 531
BseX3I CGGCCG 1 cut(s) 522
BseXI GCAGC 3 cut(s) 312, 321, 384
Bsh1285I CGRYCG 1 cut(s) 525
BshFI GGCC 2 cut(s) 524, 672
BsiEI CGRYCG 1 cut(s) 525
BsiSI CCGG 7 cut(s) 72, 101, 146, 292, 652, 673, 685
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 1 cut(s) 354
BsmFI GGGAC 1 cut(s) 634
BsnI GGCC 2 cut(s) 524, 672
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 3 cut(s) 166, 573, 583
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 2 cut(s) 186, 655
BspANI GGCC 2 cut(s) 524, 672
BspCNI CTCAG 3 cut(s) 164, 397, 514
BspLI GGNNCC 1 cut(s) 726
BspMI ACCTGC 1 cut(s) 622
BspPI GGATC 2 cut(s) 161, 591
BsrDI GCAATG 1 cut(s) 726
BsrI ACTGG 1 cut(s) 585
BssECI CCNNGG 2 cut(s) 42, 144
BssMI GATC 3 cut(s) 166, 573, 583
BssT1I CCWWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 353, 643
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 3 cut(s) 151, 405, 501
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 3 cut(s) 38, 434, 728
BstKTI GATC 3 cut(s) 169, 576, 586
BstMBI GATC 3 cut(s) 166, 573, 583
BstMCI CGRYCG 1 cut(s) 525
BstMWI GCNNNNNNNGC 6 cut(s) 72, 288, 297, 306, 309, 369
BstNSI RCATGY 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 144, 650
BstSLI GKGCMC 1 cut(s) 531
BstV1I GCAGC 3 cut(s) 312, 321, 384
BstV2I GAAGAC 1 cut(s) 644
BstZI CGGCCG 1 cut(s) 522
BsuRI GGCC 2 cut(s) 524, 672
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 3 cut(s) 38, 434, 728
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 382
BveI ACCTGC 1 cut(s) 622
Cac8I GCNNGC 1 cut(s) 537
Cfr13I GGNCC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 741
CviAII CATG 4 cut(s) 43, 107, 224, 325
CviQI GTAC 1 cut(s) 741
DdeI CTNAG 3 cut(s) 151, 405, 501
DpnI GATC 3 cut(s) 168, 575, 585
DpnII GATC 3 cut(s) 166, 573, 583
DrdI GACNNNNNNGTC 1 cut(s) 646
DseDI GACNNNNNNGTC 1 cut(s) 646
EaeI YGGCCR 2 cut(s) 522, 670
EagI CGGCCG 1 cut(s) 522
EciI GGCGGA 1 cut(s) 670
EclXI CGGCCG 1 cut(s) 522
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 103
Eco52I CGGCCG 1 cut(s) 522
EcoRI GAATTC 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 4 cut(s) 46, 110, 227, 328
FaiI YATR 7 cut(s) 44, 108, 225, 260, 326, 492, 518
FaqI GGGAC 1 cut(s) 634
FatI CATG 4 cut(s) 42, 106, 223, 324
FauI CCCGC 1 cut(s) 193
Fnu4HI GCNGC 3 cut(s) 301, 310, 373
FokI GGATG 3 cut(s) 25, 421, 715
Fsp4HI GCNGC 3 cut(s) 301, 310, 373
FspBI CTAG 2 cut(s) 93, 381
GluI GCNGC 3 cut(s) 301, 310, 373
GsuI CTGGAG 1 cut(s) 142
HaeIII GGCC 2 cut(s) 524, 672
HapII CCGG 7 cut(s) 72, 101, 146, 292, 652, 673, 685
Hin1II CATG 4 cut(s) 46, 110, 227, 328
HinfI GANTC 4 cut(s) 116, 149, 179, 317
HpaII CCGG 7 cut(s) 72, 101, 146, 292, 652, 673, 685
HphI GGTGA 2 cut(s) 133, 646
Hpy188I TCNGA 5 cut(s) 121, 406, 439, 504, 564
Hpy188III TCNNGA 2 cut(s) 333, 764
Hpy99I CGWCG 1 cut(s) 334
HpyAV CCTTC 2 cut(s) 97, 238
HpyCH4III ACNGT 2 cut(s) 353, 643
HpyCH4V TGCA 4 cut(s) 4, 138, 174, 705
HpyF10VI GCNNNNNNNGC 6 cut(s) 72, 288, 297, 306, 309, 369
HpyF3I CTNAG 3 cut(s) 151, 405, 501
Hsp92II CATG 4 cut(s) 46, 110, 227, 328
Kzo9I GATC 3 cut(s) 166, 573, 583
LmnI GCTCC 2 cut(s) 71, 724
Lsp1109I GCAGC 3 cut(s) 312, 321, 384
LweI GCATC 1 cut(s) 147
MaeI CTAG 2 cut(s) 93, 381
MaeIII GTNAC 1 cut(s) 713
MalI GATC 3 cut(s) 168, 575, 585
MboI GATC 3 cut(s) 166, 573, 583
MboII GAAGA 4 cut(s) 66, 577, 631, 649
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 6 cut(s) 428, 496, 512, 556, 756, 768
MlyI GAGTC 4 cut(s) 110, 143, 173, 311
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 8 cut(s) 27, 33, 79, 166, 204, 212, 498, 669
MseI TTAA 3 cut(s) 545, 612, 759
MspI CCGG 7 cut(s) 72, 101, 146, 292, 652, 673, 685
MspR9I CCNGG 2 cut(s) 146, 652
MwoI GCNNNNNNNGC 6 cut(s) 72, 288, 297, 306, 309, 369
NciI CCSGG 2 cut(s) 146, 652
NcoI CCATGG 1 cut(s) 42
NdeII GATC 3 cut(s) 166, 573, 583
NlaIII CATG 4 cut(s) 46, 110, 227, 328
NlaIV GGNNCC 1 cut(s) 726
NmuCI GTSAC 1 cut(s) 713
NspI RCATGY 1 cut(s) 227
PaqCI CACCTGC 1 cut(s) 622
PkrI GCNGC 3 cut(s) 302, 311, 374
PleI GAGTC 4 cut(s) 110, 143, 173, 311
PpsI GAGTC 4 cut(s) 110, 143, 173, 311
PshBI ATTAAT 1 cut(s) 759
PspN4I GGNNCC 1 cut(s) 726
PspPI GGNCC 1 cut(s) 103
RsaI GTAC 1 cut(s) 742
RsaNI GTAC 1 cut(s) 741
SaqAI TTAA 3 cut(s) 545, 612, 759
SatI GCNGC 3 cut(s) 301, 310, 373
Sau3AI GATC 3 cut(s) 166, 573, 583
Sau96I GGNCC 1 cut(s) 103
ScaI AGTACT 1 cut(s) 742
SchI GAGTC 4 cut(s) 110, 143, 173, 311
ScrFI CCNGG 2 cut(s) 146, 652
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 1 cut(s) 147
SinI GGWCC 1 cut(s) 103
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 6 cut(s) 428, 496, 512, 556, 756, 768
SsiI CCGC 2 cut(s) 186, 655
SspI AATATT 1 cut(s) 472
SspMI CTAG 2 cut(s) 93, 381
StyD4I CCNGG 2 cut(s) 144, 650
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 353, 643
TaqI TCGA 6 cut(s) 52, 126, 267, 332, 576, 698
TasI AATT 6 cut(s) 428, 496, 512, 556, 756, 768
TatI WGTACW 1 cut(s) 740
Tru1I TTAA 3 cut(s) 545, 612, 759
Tru9I TTAA 3 cut(s) 545, 612, 759
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 3 cut(s) 300, 309, 372
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 1 cut(s) 505
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 103
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 1 cut(s) 496
XceI RCATGY 1 cut(s) 227
XspI CTAG 2 cut(s) 93, 381
ZrmI AGTACT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.