MD03G1230800.v1.1

MAP kinase kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015294
Physical Location & Seq
Forward (+)
31616237 .. 31616971
735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1230800.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGAAAACCGCCGTAAAAAAAGCTCCTCCGCCGACGCATTCTCCTTCCCGAGCACTCCGATCCACGACCAAGACTCCAACTTTGAGTTCGGGTGCTTCACCCCAGACTCGGCCTCCAGCACTGGTCCCTGCAAAGACTCCCCCGCAGACCACTTGTTCCTCAATGGAAGGCTCTTGCCACATGCCTTCCCATTTCAACCTATTAGCAACGGCTTTATGGTCGTTGATAATATTTCTCGGAGAACAAGCCGGACGAGCAGCATTAGCAGCAAGGACTCTCTCATGTCGTCGAGAAGCAATAGCACCAACAGTAGGAGTAGCAGTTGTAGTAGCAGTGCTAGGACAAGCTCAAGTGACAATTCAGAGAGGAAGTTCGTGTATCACTCCAAATTATCATCCGACAGATACAAAGGCAACAAACCTAATAACAATATTTCAAGTCATCAAGTTTATGGGAATTCTCAGAGGTGGCAATTCATAACGGCAGTGCCCGTGCCGGCTTCTTTAAGCCGTAATAATTCGCGGAAGAAAGTGATCAAAGTGGATCAGCTTGAAAGGAAAGGGAAATCCGTTAAGGCGAAGAAAAATAGCCGGGTGAAGACGGTGGTGTTCCGGCGGAGATTCTTATATGGCCGGAGGTTTTTCCGGTGGCTTGTTTCGACGTGTAAAAAATGTCACGCAATGGAGCCATCTAAGAAAAGTACTAATGATAATGTAATTAAAGCTGGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

245

Amino Acids

27.47

Weight (kDa)

10.5

Isoelectric Point (pI)

62.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53163 AT3G15250
fragaria_vesca FvH4_3g19630
malus_domestica MD03G1230800.v1.1
prunus_persica Prupe.4G177000_v2.0.a1
pyrus_communis pycom11g22350
rosa_chinensis RchiOBHm_Chr5g0032821
rosa_laevigata RLG00000033431
rosa_multiflora Rmu_sc0000986.1_g000045
rosa_roxburghii Rroxscaffold_1G00047230
rosa_rugosa Rorug05G0134100
rosa_samantha Rh5AG226700 Rh5BG227800 Rh5CG255700 Rh5DG232600
rosa_wichuraiana Rw5G020800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 523
AciI CCGC 5 cut(s) 10, 30, 144, 523, 616
AclWI GGATC 2 cut(s) 55, 552
AcoI YGGCCR 1 cut(s) 631
AcsI RAATTY 1 cut(s) 457
AfaI GTAC 1 cut(s) 703
AfiI CCNNNNNNNGG 2 cut(s) 109, 312
AflIII ACRYGT 1 cut(s) 662
AgsI TTSAA 3 cut(s) 197, 438, 554
AjiI CACGTC 1 cut(s) 663
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 4 cut(s) 24, 348, 550, 725
AluI AGCT 4 cut(s) 24, 348, 550, 725
Alw21I GWGCWC 1 cut(s) 56
AlwI GGATC 2 cut(s) 55, 552
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 2 cut(s) 111, 631
ApeKI GCWGC 2 cut(s) 258, 267
ApoI RAATTY 1 cut(s) 457
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 2 cut(s) 91, 607
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 270, 279
BccI CCATC 1 cut(s) 697
BceAI ACGGC 3 cut(s) 226, 495, 498
BclI TGATCA 1 cut(s) 534
BcnI CCSGG 1 cut(s) 593
BfaI CTAG 1 cut(s) 339
BisI GCNGC 2 cut(s) 259, 268
BlsI GCNGC 2 cut(s) 260, 269
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 1 cut(s) 593
Bme18I GGWCC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 663
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 127, 687
BmrFI CCNGG 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 334
BpuMI CCSGG 1 cut(s) 593
BsaWI WCCGGW 1 cut(s) 645
BsaXI ACNNNNNCTCC 8 cut(s) 26, 56, 59, 89, 98, 128, 307, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 312
Bse118I RCCGGY 1 cut(s) 496
Bse1I ACTGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 687
BseGI GGATG 1 cut(s) 395
BseLI CCNNNNNNNGG 2 cut(s) 109, 312
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 1 cut(s) 127
BseRI GAGGAG 1 cut(s) 16
BseSI GKGCMC 1 cut(s) 492
BseXI GCAGC 2 cut(s) 270, 279
Bsh1236I CGCG 1 cut(s) 523
BshFI GGCC 2 cut(s) 113, 633
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 6 cut(s) 250, 497, 592, 613, 634, 646
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 2 cut(s) 109, 312
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 2 cut(s) 113, 633
BsoBI CYCGRG 1 cut(s) 49
Bsp1286I GDGCHC 2 cut(s) 56, 492
Bsp143I GATC 3 cut(s) 60, 534, 544
BspACI CCGC 5 cut(s) 10, 30, 144, 523, 616
BspANI GGCC 2 cut(s) 113, 633
BspCNI CTCAG 1 cut(s) 475
BspFNI CGCG 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 127, 687
BspPI GGATC 2 cut(s) 55, 552
BsrDI GCAATG 1 cut(s) 687
BsrFI RCCGGY 1 cut(s) 496
BsrI ACTGG 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 496
BssMI GATC 3 cut(s) 60, 534, 544
Bst4CI ACNGT 2 cut(s) 311, 604
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 462, 693
BstF5I GGATG 1 cut(s) 395
BstFNI CGCG 1 cut(s) 523
BstKTI GATC 3 cut(s) 63, 537, 547
BstMBI GATC 3 cut(s) 60, 534, 544
BstMWI GCNNNNNNNGC 3 cut(s) 255, 264, 267
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 591
BstSLI GKGCMC 1 cut(s) 492
BstUI CGCG 1 cut(s) 523
BstV1I GCAGC 2 cut(s) 270, 279
BstV2I GAAGAC 1 cut(s) 605
BsuRI GGCC 2 cut(s) 113, 633
BtrI CACGTC 1 cut(s) 663
BtsCI GGATG 1 cut(s) 395
BtsI GCAGTG 2 cut(s) 340, 492
BtsIMutI CAGTG 3 cut(s) 120, 340, 492
Cac8I GCNNGC 1 cut(s) 498
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 702
CviAII CATG 2 cut(s) 182, 283
CviQI GTAC 1 cut(s) 702
DdeI CTNAG 2 cut(s) 462, 693
DpnI GATC 3 cut(s) 62, 536, 546
DpnII GATC 3 cut(s) 60, 534, 544
EaeI YGGCCR 1 cut(s) 631
EciI GGCGGA 2 cut(s) 19, 631
Eco47I GGWCC 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 457
FaeI CATG 2 cut(s) 185, 286
FaiI YATR 7 cut(s) 183, 218, 284, 453, 479, 628, 630
FaqI GGGAC 1 cut(s) 111
FatI CATG 2 cut(s) 181, 282
FauI CCCGC 1 cut(s) 151
FbaI TGATCA 1 cut(s) 534
Fnu4HI GCNGC 2 cut(s) 259, 268
FokI GGATG 1 cut(s) 382
Fsp4HI GCNGC 2 cut(s) 259, 268
FspBI CTAG 1 cut(s) 339
GluI GCNGC 2 cut(s) 259, 268
GsuI CTGGAG 1 cut(s) 100
HaeIII GGCC 2 cut(s) 113, 633
HapII CCGG 6 cut(s) 250, 497, 592, 613, 634, 646
HgaI GACGC 1 cut(s) 44
Hin1II CATG 2 cut(s) 185, 286
HinfI GANTC 5 cut(s) 74, 107, 137, 275, 621
HpaII CCGG 6 cut(s) 250, 497, 592, 613, 634, 646
HphI GGTGA 2 cut(s) 91, 607
Hpy188I TCNGA 5 cut(s) 60, 240, 364, 400, 465
Hpy188III TCNNGA 2 cut(s) 49, 291
Hpy99I CGWCG 3 cut(s) 38, 292, 664
HpyAV CCTTC 3 cut(s) 55, 162, 196
HpyCH4III ACNGT 2 cut(s) 311, 604
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 264, 267
HpyF3I CTNAG 2 cut(s) 462, 693
HpySE526I ACGT 1 cut(s) 662
Hsp92II CATG 2 cut(s) 185, 286
KroI GCCGGC 1 cut(s) 496
KroNI GCCGGC 1 cut(s) 498
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 3 cut(s) 60, 534, 544
LmnI GCTCC 2 cut(s) 29, 685
Lsp1109I GCAGC 2 cut(s) 270, 279
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 2 cut(s) 353, 674
MalI GATC 3 cut(s) 62, 536, 546
MboI GATC 3 cut(s) 60, 534, 544
MboII GAAGA 3 cut(s) 538, 592, 610
MhlI GDGCHC 2 cut(s) 56, 492
MluCI AATT 6 cut(s) 358, 389, 457, 473, 517, 717
MlyI GAGTC 4 cut(s) 68, 101, 131, 269
MmeI TCCRAC 2 cut(s) 102, 423
MnlI CCTC 6 cut(s) 37, 124, 170, 360, 459, 630
MroNI GCCGGC 1 cut(s) 496
MseI TTAA 3 cut(s) 506, 573, 720
MspI CCGG 6 cut(s) 250, 497, 592, 613, 634, 646
MspR9I CCNGG 1 cut(s) 593
Mva1269I GAATGC 1 cut(s) 38
MvnI CGCG 1 cut(s) 523
MwoI GCNNNNNNNGC 3 cut(s) 255, 264, 267
NaeI GCCGGC 1 cut(s) 498
NciI CCSGG 1 cut(s) 593
NdeII GATC 3 cut(s) 60, 534, 544
NgoMIV GCCGGC 1 cut(s) 496
NlaIII CATG 2 cut(s) 185, 286
NlaIV GGNNCC 2 cut(s) 127, 687
NmeAIII GCCGAG 1 cut(s) 89
NmuCI GTSAC 2 cut(s) 353, 674
NspI RCATGY 1 cut(s) 185
PctI GAATGC 1 cut(s) 38
PdiI GCCGGC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 621
PkrI GCNGC 2 cut(s) 260, 269
PleI GAGTC 4 cut(s) 68, 101, 131, 269
PpsI GAGTC 4 cut(s) 68, 101, 131, 269
PspN4I GGNNCC 2 cut(s) 127, 687
PspPI GGNCC 1 cut(s) 125
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
SaqAI TTAA 3 cut(s) 506, 573, 720
SatI GCNGC 2 cut(s) 259, 268
Sau3AI GATC 3 cut(s) 60, 534, 544
Sau96I GGNCC 1 cut(s) 125
ScaI AGTACT 1 cut(s) 703
SchI GAGTC 4 cut(s) 68, 101, 131, 269
ScrFI CCNGG 1 cut(s) 593
SduI GDGCHC 2 cut(s) 56, 492
SetI ASST 9 cut(s) 26, 202, 350, 424, 470, 552, 641, 665, 727
SinI GGWCC 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 6 cut(s) 358, 389, 457, 473, 517, 717
SsiI CCGC 5 cut(s) 10, 30, 144, 523, 616
SspI AATATT 2 cut(s) 232, 433
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 591
TaaI ACNGT 2 cut(s) 311, 604
TaiI ACGT 1 cut(s) 665
TaqI TCGA 2 cut(s) 290, 659
TasI AATT 6 cut(s) 358, 389, 457, 473, 517, 717
TatI WGTACW 1 cut(s) 701
TfiI GAWTC 1 cut(s) 621
Tru1I TTAA 3 cut(s) 506, 573, 720
Tru9I TTAA 3 cut(s) 506, 573, 720
TscAI CASTG 3 cut(s) 127, 340, 492
TseFI GTSAC 2 cut(s) 353, 674
TseI GCWGC 2 cut(s) 258, 267
Tsp45I GTSAC 2 cut(s) 353, 674
TspDTI ATGAA 1 cut(s) 466
TspGWI ACGGA 1 cut(s) 559
TspRI CASTG 3 cut(s) 127, 340, 492
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 1 cut(s) 457
XceI RCATGY 1 cut(s) 185
XspI CTAG 1 cut(s) 339
ZrmI AGTACT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.