RLG00000033431

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015294
Physical Location & Seq
Reverse (-)
27071293 .. 27072222
930 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033431

Sequence Viewer

Length: 750 bp
ATGGAAAACGGCCAGAAAAAGAGCTCCTCCGGCGACACATTTTCCTTCCCAAGCACACCGGTCCGCGACCAAGACTCCGAAGACTTCGAATTCGGCTGCTACACACCCGACTCTGCCTCCAGTGACCCCTGCAAGGACTCCCCAGCAGACCATTTGTTCTTCAATGGCAAACTCCTACCTCATGCCTTCCCTTTTCAACCAAACAATAATGGCATGCTACTTGTCGTTGATCACTCTCGTCGCACAAGCCGAACGAACAGCATTAGCAGCAAGGACTCGCTTTCGCTTGTGTCCTCAAGAAGCAATAGCACTAACAGTAGAAGCAGCACTAGCAGTTGCAGCAGCAGCGCAAGGACAAGTTCAAGTGACAATTCTGAGAGGAAGTTGATGCATCATCATCACTACAGCAGAACGACACCGTTTAGATCATCGGACAGATACAAAACCGGAGGTGGTGCACAACTTTATGGATCTTCTCAGAGATGGCAATTCATAACACCTGTGCCGACTTTAAGCCGGGACACTTCAGTTAAGAAGAAAGTGGAGATTGCTGCAGCTGGGAAAGGAAAACCCAACAAAGAAAAGAAGAAGAGGAAGGTGAAGACGGTTGTGGTCCGGCGCCGGAGGGTCTGCCGGAGGTTTTTCCGGTGGCTGGTTTCGGCCTGCATAAAGTGTCACGCAATGGAGCCATCCAAGAAAAGTGATGTTGTTGATCATGTAAAAGATGGGAATCATGTGAAGCTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

27.86

Weight (kDa)

10.05

Isoelectric Point (pI)

66.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53163 AT3G15250
fragaria_vesca FvH4_3g19630
malus_domestica MD03G1230800.v1.1
prunus_persica Prupe.4G177000_v2.0.a1
pyrus_communis pycom11g22350
rosa_chinensis RchiOBHm_Chr5g0032821
rosa_laevigata RLG00000033431
rosa_multiflora Rmu_sc0000986.1_g000045
rosa_roxburghii Rroxscaffold_1G00047230
rosa_rugosa Rorug05G0134100
rosa_samantha Rh5AG226700 Rh5BG227800 Rh5CG255700 Rh5DG232600
rosa_wichuraiana Rw5G020800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 618
AccII CGCG 1 cut(s) 66
AciI CCGC 1 cut(s) 64
AclWI GGATC 1 cut(s) 478
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 619
AfiI CCNNNNNNNGG 2 cut(s) 133, 652
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 3 cut(s) 163, 197, 363
AluBI AGCT 3 cut(s) 24, 557, 742
AluI AGCT 3 cut(s) 24, 557, 742
Alw21I GWGCWC 2 cut(s) 26, 460
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 1 cut(s) 478
AoxI GGCC 2 cut(s) 10, 660
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 8 cut(s) 96, 267, 324, 339, 342, 345, 551, 554
ApoI RAATTY 1 cut(s) 89
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AsiGI ACCGGT 1 cut(s) 58
AspLEI GCGC 2 cut(s) 350, 621
AspS9I GGNCC 2 cut(s) 61, 613
AsuC2I CCSGG 1 cut(s) 518
AsuHPI GGTGA 1 cut(s) 610
AsuII TTCGAA 1 cut(s) 87
AvaII GGWCC 2 cut(s) 61, 613
BaeGI GKGCMC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 618
BanII GRGCYC 1 cut(s) 26
BbsI GAAGAC 2 cut(s) 87, 608
Bbv12I GWGCWC 2 cut(s) 26, 460
BbvI GCAGC 8 cut(s) 83, 279, 336, 351, 354, 357, 538, 566
BccI CCATC 3 cut(s) 477, 697, 719
BceAI ACGGC 1 cut(s) 25
BclI TGATCA 2 cut(s) 229, 712
BcnI CCSGG 1 cut(s) 518
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 2 cut(s) 403, 552
BfoI RGCGCY 1 cut(s) 622
BisI GCNGC 8 cut(s) 97, 268, 325, 340, 343, 346, 552, 555
BlsI GCNGC 8 cut(s) 98, 269, 326, 341, 344, 347, 553, 556
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 2 cut(s) 61, 613
BmgT120I GGNCC 2 cut(s) 61, 613
BmiI GGNNCC 2 cut(s) 620, 687
BmrFI CCNGG 1 cut(s) 518
BmsI GCATC 2 cut(s) 378, 400
BpiI GAAGAC 2 cut(s) 87, 608
BpmI CTGGAG 1 cut(s) 103
Bpu14I TTCGAA 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 280
BpuMI CCSGG 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 729
BsaHI GRCGYC 1 cut(s) 619
BsaWI WCCGGW 3 cut(s) 58, 446, 645
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 652
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 687
Bse8I GATNNNNATC 1 cut(s) 729
BseGI GGATG 1 cut(s) 689
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 2 cut(s) 133, 652
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 2 cut(s) 366, 491
BseNI ACTGG 1 cut(s) 120
BseRI GAGGAG 1 cut(s) 16
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 8 cut(s) 83, 279, 336, 351, 354, 357, 538, 566
BseYI CCCAGC 2 cut(s) 142, 557
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 2 cut(s) 12, 662
BshNI GGYRCC 1 cut(s) 618
BshTI ACCGGT 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 26, 460
BsiSI CCGG 8 cut(s) 30, 59, 447, 517, 616, 622, 634, 646
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 2 cut(s) 133, 652
BsmFI GGGAC 1 cut(s) 533
BsnI GGCC 2 cut(s) 12, 662
Bsp119I TTCGAA 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 26, 460
Bsp143I GATC 4 cut(s) 229, 425, 470, 712
BspACI CCGC 1 cut(s) 64
BspANI GGCC 2 cut(s) 12, 662
BspCNI CTCAG 2 cut(s) 367, 490
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 620, 687
BspMAI CTGCAG 1 cut(s) 556
BspPI GGATC 1 cut(s) 478
BspT104I TTCGAA 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 618
BsrDI GCAATG 1 cut(s) 687
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 58
BssMI GATC 4 cut(s) 229, 425, 470, 712
BssNI GRCGYC 1 cut(s) 619
Bst4CI ACNGT 3 cut(s) 317, 420, 607
Bst6I CTCTTC 1 cut(s) 584
BstACI GRCGYC 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 2 cut(s) 215, 664
BstDEI CTNAG 2 cut(s) 375, 477
BstF5I GGATG 1 cut(s) 689
BstFNI CGCG 1 cut(s) 66
BstH2I RGCGCY 1 cut(s) 622
BstHHI GCGC 2 cut(s) 350, 621
BstKTI GATC 4 cut(s) 232, 428, 473, 715
BstMBI GATC 4 cut(s) 229, 425, 470, 712
BstMWI GCNNNNNNNGC 5 cut(s) 30, 267, 330, 339, 345
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 1 cut(s) 516
BstSFI CTRYAG 2 cut(s) 403, 552
BstSLI GKGCMC 1 cut(s) 460
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 8 cut(s) 83, 279, 336, 351, 354, 357, 538, 566
BstV2I GAAGAC 2 cut(s) 87, 608
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 2 cut(s) 12, 662
BtsCI GGATG 1 cut(s) 689
BtsIMutI CAGTG 1 cut(s) 127
Cac8I GCNNGC 2 cut(s) 215, 664
CfoI GCGC 2 cut(s) 350, 621
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 61, 613
CpoI CGGWCCG 1 cut(s) 61
CspAI ACCGGT 1 cut(s) 58
CspI CGGWCCG 1 cut(s) 61
CviAII CATG 4 cut(s) 182, 214, 716, 734
DdeI CTNAG 2 cut(s) 375, 477
DinI GGCGCC 1 cut(s) 620
DpnI GATC 4 cut(s) 231, 427, 472, 714
DpnII GATC 4 cut(s) 229, 425, 470, 712
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 2 cut(s) 61, 613
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 89
EcoT22I ATGCAT 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 26
EgeI GGCGCC 1 cut(s) 620
EheI GGCGCC 1 cut(s) 620
FaeI CATG 4 cut(s) 185, 217, 719, 737
FaiI YATR 7 cut(s) 183, 215, 468, 494, 668, 717, 735
FaqI GGGAC 1 cut(s) 533
FatI CATG 4 cut(s) 181, 213, 715, 733
FbaI TGATCA 2 cut(s) 229, 712
Fnu4HI GCNGC 8 cut(s) 97, 268, 325, 340, 343, 346, 552, 555
FokI GGATG 1 cut(s) 676
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 8 cut(s) 97, 268, 325, 340, 343, 346, 552, 555
FspBI CTAG 1 cut(s) 330
GlaI GCGC 2 cut(s) 349, 620
GluI GCNGC 8 cut(s) 97, 268, 325, 340, 343, 346, 552, 555
GsaI CCCAGC 2 cut(s) 146, 561
GsuI CTGGAG 1 cut(s) 103
HaeII RGCGCY 1 cut(s) 622
HaeIII GGCC 2 cut(s) 12, 662
HapII CCGG 8 cut(s) 30, 59, 447, 517, 616, 622, 634, 646
HhaI GCGC 2 cut(s) 350, 621
Hin1I GRCGYC 1 cut(s) 619
Hin1II CATG 4 cut(s) 185, 217, 719, 737
Hin6I GCGC 2 cut(s) 348, 619
HinP1I GCGC 2 cut(s) 348, 619
HinfI GANTC 5 cut(s) 74, 110, 137, 275, 730
HpaII CCGG 8 cut(s) 30, 59, 447, 517, 616, 622, 634, 646
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 1 cut(s) 458
Hpy188I TCNGA 4 cut(s) 79, 376, 433, 480
Hpy188III TCNNGA 1 cut(s) 297
Hpy8I GTNNAC 1 cut(s) 458
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 3 cut(s) 55, 196, 589
HpyCH4III ACNGT 3 cut(s) 317, 420, 607
HpyCH4V TGCA 6 cut(s) 132, 339, 391, 458, 554, 666
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 267, 330, 339, 345
HpyF3I CTNAG 2 cut(s) 375, 477
Hsp92I GRCGYC 1 cut(s) 619
Hsp92II CATG 4 cut(s) 185, 217, 719, 737
HspAI GCGC 2 cut(s) 348, 619
KasI GGCGCC 1 cut(s) 618
Ksp22I TGATCA 2 cut(s) 229, 712
Kzo9I GATC 4 cut(s) 229, 425, 470, 712
LmnI GCTCC 2 cut(s) 29, 685
Lsp1109I GCAGC 8 cut(s) 83, 279, 336, 351, 354, 357, 538, 566
LweI GCATC 2 cut(s) 378, 400
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 3 cut(s) 122, 365, 674
MalI GATC 4 cut(s) 231, 427, 472, 714
MboI GATC 4 cut(s) 229, 425, 470, 712
MboII GAAGA 7 cut(s) 92, 151, 465, 547, 598, 601, 613
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 2 cut(s) 26, 460
MluCI AATT 3 cut(s) 89, 370, 488
Mly113I GGCGCC 1 cut(s) 619
MlyI GAGTC 4 cut(s) 68, 104, 131, 269
MnlI CCTC 9 cut(s) 37, 127, 189, 304, 372, 443, 585, 618, 630
Mph1103I ATGCAT 1 cut(s) 393
MseI TTAA 2 cut(s) 512, 531
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 8 cut(s) 30, 59, 447, 517, 616, 622, 634, 646
MspR9I CCNGG 1 cut(s) 518
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 5 cut(s) 30, 267, 330, 339, 345
NarI GGCGCC 1 cut(s) 619
NciI CCSGG 1 cut(s) 518
NdeII GATC 4 cut(s) 229, 425, 470, 712
NlaIII CATG 4 cut(s) 185, 217, 719, 737
NlaIV GGNNCC 2 cut(s) 620, 687
NmuCI GTSAC 3 cut(s) 122, 365, 674
NsiI ATGCAT 1 cut(s) 393
NspI RCATGY 1 cut(s) 217
NspV TTCGAA 1 cut(s) 87
PaeI GCATGC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 247
PfeI GAWTC 1 cut(s) 730
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 8 cut(s) 98, 269, 326, 341, 344, 347, 553, 556
PleI GAGTC 4 cut(s) 68, 104, 131, 269
PluTI GGCGCC 1 cut(s) 622
PpsI GAGTC 4 cut(s) 68, 104, 131, 269
Psp124BI GAGCTC 1 cut(s) 26
PspFI CCCAGC 2 cut(s) 142, 557
PspN4I GGNNCC 2 cut(s) 620, 687
PspPI GGNCC 2 cut(s) 61, 613
PstI CTGCAG 1 cut(s) 556
PsuI RGATCY 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 557
Rsr2I CGGWCCG 1 cut(s) 61
RsrII CGGWCCG 1 cut(s) 61
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 512, 531
SatI GCNGC 8 cut(s) 97, 268, 325, 340, 343, 346, 552, 555
Sau3AI GATC 4 cut(s) 229, 425, 470, 712
Sau96I GGNCC 2 cut(s) 61, 613
SchI GAGTC 4 cut(s) 68, 104, 131, 269
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 2 cut(s) 26, 460
SetI ASST 8 cut(s) 26, 181, 454, 502, 559, 600, 641, 744
SfaNI GCATC 2 cut(s) 378, 400
SfcI CTRYAG 2 cut(s) 403, 552
SfoI GGCGCC 1 cut(s) 620
SfuI TTCGAA 1 cut(s) 87
SinI GGWCC 2 cut(s) 61, 613
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
SphI GCATGC 1 cut(s) 217
Sse9I AATT 3 cut(s) 89, 370, 488
SsiI CCGC 1 cut(s) 64
SspDI GGCGCC 1 cut(s) 618
SspMI CTAG 1 cut(s) 330
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 516
TaaI ACNGT 3 cut(s) 317, 420, 607
TaqI TCGA 1 cut(s) 87
TasI AATT 3 cut(s) 89, 370, 488
TfiI GAWTC 1 cut(s) 730
Tru1I TTAA 2 cut(s) 512, 531
Tru9I TTAA 2 cut(s) 512, 531
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 3 cut(s) 122, 365, 674
TseI GCWGC 8 cut(s) 96, 267, 324, 339, 342, 345, 551, 554
Tsp45I GTSAC 3 cut(s) 122, 365, 674
TspDTI ATGAA 1 cut(s) 481
TspRI CASTG 1 cut(s) 127
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 2 cut(s) 61, 613
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 217
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.