Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008560

Physical Location & Seq

Genomic Coordinates Reverse (-)

20246026 .. 20250345

Gene Structure 4320 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G54220.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGGCTTATGCGTCACGCATCATTAATCATTCCAAGAAGTTGAAGGATGTTTCCACTTTACTACGCCGTGAGAATGCTGCAACGATCCGCTACTACTCGAATACTAATCGAGCTCCTCTGAACAGAGAAGATACTTTCAATTCCCGTCTTGGTTATCCCCCACTGGAGAGGATTTCCATATGTAGCACCAGCACTTTACCGGTGTCCATTATTTTTTCAACTACGAGATCAAATCTAAGCAGTGCAATGGGAAGGCCAATATTTGGAAAAGAATTTTCATGTTTGATGCAATCAGCTAGAGGATTTTCATCGGGTTCAGATCTGCCTCCTCATCAAGAGATCGGGATGCCTTCTCTCTCGCCAACAATGACTGAGGGTAACATCGCCAGGTGGCTGAAAAAAGAAGGTGATAAAGTTGCTCCTGGGGAAGTACTCTGTGAAGTTGAAACTGACAAAGCAACTGTCGAAATGGAATGCATGGAAGAGGGCTATCTCGCCAAGATTGTAAAGGCGGAAGGTTCAAAAGAAATTCAAGTCGGCGAGGTAATTGCTATTACAGTTGAAGATGAAGAGGACATTGGAAAGTTCAAAGATTACACCCCATCATCTACTGCTGATGCCGCTCCTACAAAAGCAGAACCAACTCCTGCCCCACCAAAGGAAGAGAAGGTCAAACAGCCATCATCGCCTCCTGAACCGAAGGCCTCCAAGCCTAGCACACCTCCCACAGGAGATCGTGTTTTTGCTAGTCCTCTTGCAAGAAAGTTGGCTGAAGATAATAACGTGCCTCTCTCAGACATCGAAGGCACAGGTCCTGAAGGACGGATAGTGAAGGCAGATATCGATGAGTATTTAGCTTCAAGTGGTAAAGGAGCTACTGCGAAGCCATCTAAGTCAACTGATTCTAAGGCCCCAGCTTTGGACTATGTTGACATCCCTCATTCTCAGATACGAAAGGTCACAGCCTCACGTTTGGCATTCTCAAAGCAAACTATCCCTCACTATTACTTAACCGTCGATACATGTGTTGACAAACTTATGGCTCTGAGGAGTCAGCTTAATTCATTCAAAGAGGCTTCTGGTGGGAAACGGATATCTGTCAATGATCTTGTTGTTAAGGCTGCTGCATTAGCTCTCAGAAAAGTTCCTCAGTGCAATAGTTCATGGACTGACGATTACATCCGCCAGTTTAAAAATGTGAACATCAACGTCGCGGTGCAGACAGAAAACGGGCTGTATGTCCCTGTTGTGAAGGACGCAGACAGGAAGGGTCTGTCCACAATTGGAGAAGAAGTTCGGTTATTAGCTCAAAAAGCAAAAGAGAACAGCTTAAAGCCAGAAGATTATGAGGGAGGAACATTTACTGTCTCTAACTTGGGAGGACCTTTTGGCATCAAGCAATTCTGTGCAGTCGTTAATCCACCTCAAGCCGCCATTCTAGCAGTTGGATCCGCTGAAAAGAGAGTTGTCCCTGGTAACGGTCCAGATCAATTCAACTTTGCTTCTTACATGCCTGTTACACTGAGCTGTGACCATCGCGTAGTAGACGGTGCCATTGGAGCTGAATGGTTGAAAGCATTTAAGGGATACATCGAGAACCCTAAATCTATGTTACTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

539

Amino Acids

58.47

Weight (kDa)

7.95

Isoelectric Point (pI)

42.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	113 - 184	4.4e-18	Biotin-requiring enzyme
E3_binding	PF02817	247 - 281	6.9e-16	e3 binding domain
2-oxoacid_dh	PF00198	309 - 539	1.5e-78	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54220 AT1G54220 AT3G13930
fragaria_vesca	FvH4_6g34140 FvH4_6g34140 FvH4_6g34140
malus_domestica	MD09G1188500.v1.1 MD17G1172600.v1.1
prunus_persica	Prupe.3G045200_v2.0.a1 Prupe.3G045200_v2.0.a1
pyrus_communis	pycom09g10560 pycom17g17050
rosa_chinensis	RchiOBHm_Chr2g0144451
rosa_laevigata	RLG00000020082
rosa_multiflora	Rmu_sc0003475.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101300
rosa_rugosa	Rorug02G0386700 Rorug02G0386800
rosa_samantha	Rh2AG439000 Rh2BG449300 Rh2CG426100 Rh2DG459100
rosa_wichuraiana	Rw2G035920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1550
AccB7I	CCANNNNNTGG	1 cut(s)	263
AccBSI	CCGCTC	1 cut(s)	623
AccI	GTMKAC	1 cut(s)	1545
AccII	CGCG	2 cut(s)	1214, 1539
AciI	CCGC	7 cut(s)	88, 512, 621, 1183, 1214, 1431, 1452
AclWI	GGATC	3 cut(s)	79, 1443, 1456
AcsI	RAATTY	2 cut(s)	272, 528
AcuI	CTGAAG	2 cut(s)	792, 837
AfaI	GTAC	1 cut(s)	432
AfiI	CCNNNNNNNGG	5 cut(s)	263, 658, 728, 919, 1478
AflIII	ACRYGT	1 cut(s)	1022
AgeI	ACCGGT	1 cut(s)	199
AjnI	CCWGG	3 cut(s)	386, 421, 1471
AjuI	GAANNNNNNNTTGG	2 cut(s)	561, 593
AloI	GAACNNNNNNTCC	2 cut(s)	1278, 1310
Alw21I	GWGCWC	1 cut(s)	115
Alw26I	GTCTC	1 cut(s)	1372
AlwI	GGATC	3 cut(s)	79, 1443, 1456
AlwNI	CAGNNNCTG	1 cut(s)	815
AoxI	GGCC	3 cut(s)	254, 702, 909
ApeKI	GCWGC	3 cut(s)	77, 1121, 1124
ApoI	RAATTY	2 cut(s)	272, 528
ArsI	GACNNNNNNTTYG	2 cut(s)	447, 479
AseI	ATTAAT	1 cut(s)	24
AsiGI	ACCGGT	1 cut(s)	199
Asp700I	GAANNNNTTC	1 cut(s)	1293
AspS9I	GGNCC	4 cut(s)	812, 910, 1382, 1481
AsuHPI	GGTGA	1 cut(s)	419
AvaII	GGWCC	3 cut(s)	812, 1382, 1481
BaeI	ACNNNNGTAYC	2 cut(s)	1011, 1044
BamHI	GGATCC	1 cut(s)	1448
BanI	GGYRCC	1 cut(s)	1550
BanII	GRGCYC	1 cut(s)	115
Bbv12I	GWGCWC	1 cut(s)	115
BbvI	GCAGC	3 cut(s)	64, 1108, 1111
BccI	CCATC	4 cut(s)	610, 688, 895, 1542
BceAI	ACGGC	1 cut(s)	51
BcgI	CGANNNNNNTGC	2 cut(s)	530, 564
BciT130I	CCWGG	3 cut(s)	388, 423, 1473
BciVI	GTATCC	1 cut(s)	1580
BcoDI	GTCTC	1 cut(s)	1372
BfaI	CTAG	4 cut(s)	297, 714, 747, 1439
BfuI	GTATCC	1 cut(s)	1580
BglII	AGATCT	1 cut(s)	319
BisI	GCNGC	5 cut(s)	78, 621, 1122, 1125, 1431
BlsI	GCNGC	5 cut(s)	79, 622, 1123, 1126, 1432
BmcAI	AGTACT	1 cut(s)	432
Bme1390I	CCNGG	3 cut(s)	388, 423, 1473
Bme18I	GGWCC	3 cut(s)	812, 1382, 1481
BmgT120I	GGNCC	4 cut(s)	812, 910, 1382, 1481
BmiI	GGNNCC	3 cut(s)	912, 1450, 1552
BmrFI	CCNGG	3 cut(s)	388, 423, 1473
BmsI	GCATC	5 cut(s)	27, 276, 336, 607, 1401
BpmI	CTGGAG	1 cut(s)	185
BpuEI	CTTGAG	1 cut(s)	1410
Bsa29I	ATCGAT	1 cut(s)	843
BsaBI	GATNNNNATC	1 cut(s)	307
BsaJI	CCNNGG	2 cut(s)	422, 1471
BsaWI	WCCGGW	1 cut(s)	199
BsaXI	ACNNNNNCTCC	4 cut(s)	723, 753, 1278, 1308
Bsc4I	CCNNNNNNNGG	5 cut(s)	263, 658, 728, 919, 1478
Bse118I	RCCGGY	1 cut(s)	199
Bse1I	ACTGG	2 cut(s)	168, 1186
Bse3DI	GCAATG	1 cut(s)	252
Bse8I	GATNNNNATC	1 cut(s)	307
BseBI	CCWGG	3 cut(s)	388, 423, 1473
BseCI	ATCGAT	1 cut(s)	843
BseDI	CCNNGG	2 cut(s)	422, 1471
BseGI	GGATG	4 cut(s)	52, 351, 933, 1179
BseJI	GATNNNNATC	1 cut(s)	307
BseLI	CCNNNNNNNGG	5 cut(s)	263, 658, 728, 919, 1478
BseMI	GCAATG	1 cut(s)	252
BseMII	CTCAG	7 cut(s)	363, 807, 959, 1037, 1150, 1163, 1514
BseNI	ACTGG	2 cut(s)	168, 1186
BseRI	GAGGAG	3 cut(s)	105, 318, 1063
BseXI	GCAGC	3 cut(s)	64, 1108, 1111
BseYI	CCCAGC	1 cut(s)	913
BsgI	GTGCAG	2 cut(s)	1238, 1428
Bsh1236I	CGCG	2 cut(s)	1214, 1539
BshFI	GGCC	3 cut(s)	256, 704, 911
BshNI	GGYRCC	1 cut(s)	1550
BshTI	ACCGGT	1 cut(s)	199
BshVI	ATCGAT	1 cut(s)	843
BsiHKAI	GWGCWC	1 cut(s)	115
BsiSI	CCGG	1 cut(s)	200
BslFI	GGGAC	2 cut(s)	1226, 1454
BslI	CCNNNNNNNGG	5 cut(s)	263, 658, 728, 919, 1478
BsmAI	GTCTC	1 cut(s)	1372
BsmFI	GGGAC	2 cut(s)	1226, 1454
BsmI	GAATGC	3 cut(s)	79, 479, 977
BsnI	GGCC	3 cut(s)	256, 704, 911
Bsp1286I	GDGCHC	1 cut(s)	115
Bsp143I	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
BspACI	CCGC	7 cut(s)	88, 512, 621, 1183, 1214, 1431, 1452
BspANI	GGCC	3 cut(s)	256, 704, 911
BspCNI	CTCAG	7 cut(s)	364, 806, 958, 1038, 1149, 1162, 1515
BspDI	ATCGAT	1 cut(s)	843
BspFNI	CGCG	2 cut(s)	1214, 1539
BspLI	GGNNCC	3 cut(s)	912, 1450, 1552
BspPI	GGATC	3 cut(s)	79, 1443, 1456
BspT107I	GGYRCC	1 cut(s)	1550
BsrBI	CCGCTC	1 cut(s)	623
BsrDI	GCAATG	1 cut(s)	252
BsrFI	RCCGGY	1 cut(s)	199
BsrI	ACTGG	2 cut(s)	168, 1186
BssAI	RCCGGY	1 cut(s)	199
BssECI	CCNNGG	2 cut(s)	422, 1471
BssMI	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
Bst2UI	CCWGG	3 cut(s)	388, 423, 1473
Bst4CI	ACNGT	6 cut(s)	463, 559, 1015, 1366, 1481, 1550
Bst6I	CTCTTC	3 cut(s)	477, 564, 657
BstENI	CCTNNNNNAGG	1 cut(s)	726
BstF5I	GGATG	4 cut(s)	52, 351, 933, 1179
BstFNI	CGCG	2 cut(s)	1214, 1539
BstKTI	GATC	8 cut(s)	87, 230, 322, 342, 736, 1108, 1451, 1489
BstMAI	GTCTC	1 cut(s)	1372
BstMBI	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
BstMWI	GCNNNNNNNGC	6 cut(s)	620, 685, 1130, 1313, 1439, 1559
BstNI	CCWGG	3 cut(s)	388, 423, 1473
BstNSI	RCATGY	2 cut(s)	1026, 1513
BstSCI	CCNGG	3 cut(s)	386, 421, 1471
BstUI	CGCG	2 cut(s)	1214, 1539
BstV1I	GCAGC	3 cut(s)	64, 1108, 1111
BstX2I	RGATCY	2 cut(s)	319, 1448
BstYI	RGATCY	2 cut(s)	319, 1448
Bsu15I	ATCGAT	1 cut(s)	843
BsuI	GTATCC	1 cut(s)	1580
BsuRI	GGCC	3 cut(s)	256, 704, 911
BsuTUI	ATCGAT	1 cut(s)	843
BtgZI	GCGATG	3 cut(s)	367, 669, 1520
BtsCI	GGATG	4 cut(s)	52, 351, 933, 1179
BtsI	GCAGTG	1 cut(s)	247
BtsIMutI	CAGTG	4 cut(s)	161, 247, 1157, 1520
CaiI	CAGNNNCTG	1 cut(s)	815
Cfr10I	RCCGGY	1 cut(s)	199
Cfr13I	GGNCC	4 cut(s)	812, 910, 1382, 1481
ClaI	ATCGAT	1 cut(s)	843
CseI	GACGC	1 cut(s)	1265
Csp6I	GTAC	1 cut(s)	431
CspAI	ACCGGT	1 cut(s)	199
CviAII	CATG	5 cut(s)	279, 478, 1023, 1164, 1510
CviQI	GTAC	1 cut(s)	431
DpnI	GATC	8 cut(s)	86, 229, 321, 341, 735, 1107, 1450, 1488
DpnII	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
DraI	TTTAAA	1 cut(s)	1192
Eam1104I	CTCTTC	3 cut(s)	477, 564, 657
EarI	CTCTTC	3 cut(s)	477, 564, 657
EciI	GGCGGA	2 cut(s)	527, 1172
Ecl136II	GAGCTC	1 cut(s)	113
Eco147I	AGGCCT	1 cut(s)	704
Eco24I	GRGCYC	1 cut(s)	115
Eco32I	GATATC	2 cut(s)	841, 1095
Eco47I	GGWCC	3 cut(s)	812, 1382, 1481
Eco53kI	GAGCTC	1 cut(s)	113
Eco57I	CTGAAG	2 cut(s)	792, 837
EcoICRI	GAGCTC	1 cut(s)	113
EcoNI	CCTNNNNNAGG	1 cut(s)	726
EcoO109I	RGGNCCY	3 cut(s)	812, 910, 1382
EcoRII	CCWGG	3 cut(s)	386, 421, 1471
EcoRV	GATATC	2 cut(s)	841, 1095
EcoT22I	ATGCAT	1 cut(s)	479
EcoT38I	GRGCYC	1 cut(s)	115
FaeI	CATG	5 cut(s)	282, 481, 1026, 1167, 1513
FaqI	GGGAC	2 cut(s)	1226, 1454
FatI	CATG	5 cut(s)	278, 477, 1022, 1163, 1509
FauNDI	CATATG	1 cut(s)	179
FblI	GTMKAC	1 cut(s)	1545
Fnu4HI	GCNGC	5 cut(s)	78, 621, 1122, 1125, 1431
FokI	GGATG	4 cut(s)	59, 358, 920, 1166
FriOI	GRGCYC	1 cut(s)	115
Fsp4HI	GCNGC	5 cut(s)	78, 621, 1122, 1125, 1431
FspBI	CTAG	4 cut(s)	297, 714, 747, 1439
GluI	GCNGC	5 cut(s)	78, 621, 1122, 1125, 1431
GsaI	CCCAGC	1 cut(s)	917
GsuI	CTGGAG	1 cut(s)	185
HaeIII	GGCC	3 cut(s)	256, 704, 911
HapII	CCGG	1 cut(s)	200
HgaI	GACGC	1 cut(s)	1265
Hin1II	CATG	5 cut(s)	282, 481, 1026, 1167, 1513
HincII	GTYRAC	3 cut(s)	897, 931, 1030
HindII	GTYRAC	3 cut(s)	897, 931, 1030
HinfI	GANTC	2 cut(s)	902, 1051
HpaII	CCGG	1 cut(s)	200
HphI	GGTGA	1 cut(s)	419
Hpy166II	GTNNAC	6 cut(s)	897, 931, 1030, 1201, 1278, 1546
Hpy188I	TCNGA	6 cut(s)	120, 319, 796, 948, 1047, 1139
Hpy188III	TCNNGA	6 cut(s)	335, 343, 692, 815, 1484, 1594
Hpy8I	GTNNAC	6 cut(s)	897, 931, 1030, 1201, 1278, 1546
Hpy99I	CGWCG	2 cut(s)	1019, 1214
HpyCH4III	ACNGT	6 cut(s)	463, 559, 1015, 1366, 1481, 1550
HpyCH4IV	ACGT	3 cut(s)	783, 970, 1209
HpyCH4V	TGCA	9 cut(s)	80, 245, 289, 477, 758, 1127, 1155, 1219, 1409
HpyF10VI	GCNNNNNNNGC	6 cut(s)	620, 685, 1130, 1313, 1439, 1559
HpySE526I	ACGT	3 cut(s)	783, 970, 1209
Hsp92II	CATG	5 cut(s)	282, 481, 1026, 1167, 1513
Kzo9I	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
LmnI	GCTCC	5 cut(s)	118, 424, 628, 872, 1559
Lsp1109I	GCAGC	3 cut(s)	64, 1108, 1111
LweI	GCATC	5 cut(s)	27, 276, 336, 607, 1401
MaeI	CTAG	4 cut(s)	297, 714, 747, 1439
MaeII	ACGT	3 cut(s)	783, 970, 1209
MaeIII	GTNAC	7 cut(s)	12, 377, 958, 1475, 1516, 1529, 1611
MalI	GATC	8 cut(s)	86, 229, 321, 341, 735, 1107, 1450, 1488
MbiI	CCGCTC	1 cut(s)	623
MboI	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
MboII	GAAGA	8 cut(s)	140, 494, 575, 581, 674, 785, 1301, 1352
MfeI	CAATTG	1 cut(s)	1281
MflI	RGATCY	2 cut(s)	319, 1448
MhlI	GDGCHC	1 cut(s)	115
MluCI	AATT	8 cut(s)	139, 272, 528, 546, 1060, 1281, 1400, 1490
MlyI	GAGTC	1 cut(s)	1060
MmeI	TCCRAC	1 cut(s)	1426
Mph1103I	ATGCAT	1 cut(s)	479
MroXI	GAANNNNTTC	1 cut(s)	1293
MseI	TTAA	8 cut(s)	24, 1010, 1059, 1116, 1191, 1331, 1416, 1581
MspA1I	CMGCKG	1 cut(s)	1454
MspI	CCGG	1 cut(s)	200
MspR9I	CCNGG	3 cut(s)	388, 423, 1473
MunI	CAATTG	1 cut(s)	1281
Mva1269I	GAATGC	3 cut(s)	79, 479, 977
MvaI	CCWGG	3 cut(s)	388, 423, 1473
MvnI	CGCG	2 cut(s)	1214, 1539
MwoI	GCNNNNNNNGC	6 cut(s)	620, 685, 1130, 1313, 1439, 1559
NdeI	CATATG	1 cut(s)	179
NdeII	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
NlaIII	CATG	5 cut(s)	282, 481, 1026, 1167, 1513
NlaIV	GGNNCC	3 cut(s)	912, 1450, 1552
NmuCI	GTSAC	3 cut(s)	12, 958, 1529
NsiI	ATGCAT	1 cut(s)	479
NspI	RCATGY	2 cut(s)	1026, 1513
PceI	AGGCCT	1 cut(s)	704
PciI	ACATGT	1 cut(s)	1022
PctI	GAATGC	3 cut(s)	79, 479, 977
PdmI	GAANNNNTTC	1 cut(s)	1293
PfeI	GAWTC	1 cut(s)	902
PflMI	CCANNNNNTGG	1 cut(s)	263
PinAI	ACCGGT	1 cut(s)	199
PkrI	GCNGC	5 cut(s)	79, 622, 1123, 1126, 1432
PleI	GAGTC	1 cut(s)	1059
PpsI	GAGTC	1 cut(s)	1059
PpuMI	RGGWCCY	2 cut(s)	812, 1382
PscI	ACATGT	1 cut(s)	1022
PshBI	ATTAAT	1 cut(s)	24
Psp124BI	GAGCTC	1 cut(s)	115
Psp5II	RGGWCCY	2 cut(s)	812, 1382
Psp6I	CCWGG	3 cut(s)	386, 421, 1471
PspFI	CCCAGC	1 cut(s)	913
PspGI	CCWGG	3 cut(s)	386, 421, 1471
PspN4I	GGNNCC	3 cut(s)	912, 1450, 1552
PspPI	GGNCC	4 cut(s)	812, 910, 1382, 1481
PspPPI	RGGWCCY	2 cut(s)	812, 1382
PstNI	CAGNNNCTG	1 cut(s)	815
PsuI	RGATCY	2 cut(s)	319, 1448
RsaI	GTAC	1 cut(s)	432
RsaNI	GTAC	1 cut(s)	431
SacI	GAGCTC	1 cut(s)	115
SaqAI	TTAA	8 cut(s)	24, 1010, 1059, 1116, 1191, 1331, 1416, 1581
SatI	GCNGC	5 cut(s)	78, 621, 1122, 1125, 1431
Sau3AI	GATC	8 cut(s)	84, 227, 319, 339, 733, 1105, 1448, 1486
Sau96I	GGNCC	4 cut(s)	812, 910, 1382, 1481
ScaI	AGTACT	1 cut(s)	432
SchI	GAGTC	1 cut(s)	1060
ScrFI	CCNGG	3 cut(s)	388, 423, 1473
SduI	GDGCHC	1 cut(s)	115
SfaNI	GCATC	5 cut(s)	27, 276, 336, 607, 1401
SinI	GGWCC	3 cut(s)	812, 1382, 1481
SmlI	CTYRAG	1 cut(s)	1425
SmoI	CTYRAG	1 cut(s)	1425
Sse9I	AATT	8 cut(s)	139, 272, 528, 546, 1060, 1281, 1400, 1490
SseBI	AGGCCT	1 cut(s)	704
SsiI	CCGC	7 cut(s)	88, 512, 621, 1183, 1214, 1431, 1452
SspI	AATATT	1 cut(s)	261
SspMI	CTAG	4 cut(s)	297, 714, 747, 1439
SstI	GAGCTC	1 cut(s)	115
StuI	AGGCCT	1 cut(s)	704
StyD4I	CCNGG	3 cut(s)	386, 421, 1471
TaaI	ACNGT	6 cut(s)	463, 559, 1015, 1366, 1481, 1550
TaiI	ACGT	3 cut(s)	786, 973, 1212
TaqI	TCGA	7 cut(s)	98, 109, 465, 801, 843, 1017, 1593
TasI	AATT	8 cut(s)	139, 272, 528, 546, 1060, 1281, 1400, 1490
TatI	WGTACW	1 cut(s)	430
TauI	GCSGC	2 cut(s)	623, 1433
TfiI	GAWTC	1 cut(s)	902
Tru1I	TTAA	8 cut(s)	24, 1010, 1059, 1116, 1191, 1331, 1416, 1581
Tru9I	TTAA	8 cut(s)	24, 1010, 1059, 1116, 1191, 1331, 1416, 1581
TscAI	CASTG	4 cut(s)	168, 247, 1157, 1527
TseFI	GTSAC	3 cut(s)	12, 958, 1529
TseI	GCWGC	3 cut(s)	77, 1121, 1124
Tsp45I	GTSAC	3 cut(s)	12, 958, 1529
TspDTI	ATGAA	5 cut(s)	267, 297, 582, 1053, 1152
TspGWI	ACGGA	2 cut(s)	838, 1105
TspRI	CASTG	4 cut(s)	168, 247, 1157, 1527
Van91I	CCANNNNNTGG	1 cut(s)	263
VpaK11BI	GGWCC	3 cut(s)	812, 1382, 1481
VspI	ATTAAT	1 cut(s)	24
XagI	CCTNNNNNAGG	1 cut(s)	726
XapI	RAATTY	2 cut(s)	272, 528
XceI	RCATGY	2 cut(s)	1026, 1513
XmiI	GTMKAC	1 cut(s)	1545
XmnI	GAANNNNTTC	1 cut(s)	1293
XspI	CTAG	4 cut(s)	297, 714, 747, 1439
ZrmI	AGTACT	1 cut(s)	432
Zsp2I	ATGCAT	1 cut(s)	479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.