Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0008560

Physical Location & Seq

Genomic Coordinates Reverse (-)

23291294 .. 23297763

Gene Structure 6470 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00101300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGTCTTACGCATCTCATATCATCCACCACTCCAAAAAGTTAAGGAATGCTCCCAACTTACTAATGCATGAACATGCCCTCTTGGTGCGTTGTTTTTCACACAATGCTCAACCCACTACTGATATATGTAAGGTTCATCGTCATGGTTTTGCGCCTTTGGGAAGAGAAAGTAGGTCTTTCAGCAGTAGCACTAAATCGCTGTCAGGTACATTCACCAGGAATGTCTCAACAACAATGAATATGGGGAGGTCAATTTCTGGATCAATATATAACAATCAAACTTCATGTTTGCAGGGACAATTGAGGAGAGGCTTTTCGTCTGATGCAGGCCTTCCTCCACACCAAGAAATTGGAATGCCTTCTCTTTCACCTACTATGTCTGAGGGTAATATTGCAAGGTGGTTGAAGAAAGAGGGTGATAAAGTCTCTCCTGGTGAAGTGCTCTGTGAAGTTGAAACTGATAAAGCTACAGTTGAGATGGAATGCATGGAGGATGGATATCTTGCCAAGATTGTACAAGGAGAAGGGGCCCAAGGAATCAAAGTTGGCGAGGTGATTTGCGTAACTGTTGAAGATGAGGAAGATATTGCAAAGTTCAAAGATTACACACCATCAGCGTCAGGTTCTGGTGGTGATGTAGCTAAAGGGTCATCTGACTCTAGCCCGCCTCCACCGCCCAAAAAAGAGGCAGTTGAAGCACCAGTTACTGCTCCCGAGCCAAAGACTTCAAAGCCCAGTGCAGCTCCTCCTTCTGGAGATCGTGTATTTGCTAGTCCTCTTGCAAGAAGTTTGGCTGAGGAACACAAGGTACCCCTCTCAAGTATCAAAGGAACAGGCCCGGATGGAAGCATTGTGAAGGCTGATATTGAAGAGTACTTGGGTATGGGTGGGAAGCAAGTTTCTGAACCAGCTCCGAAGGCTAAGGGTGCTGCAGCCCCAGCACTGGATTACACGGACATCCCTCATACTCAGATACGGAAGGTTACAGCTTCAAGATTATTATTCTCAAAGCAAACTATTCCTCATTATTATTTGACAGTAGATACATGTGTCGACAAGCTCGTTGAGTTGAGAGGCCATCTCAATTCATTGCAAGAAGCTACAGGTGGGAAGCGGATATCTATCAATGATCTTGTAATAAAGGCTGCTGCTTTGGCTCTTCAAAAGGTTCCTCAGGTCAATAGCTCATGGACAGATGATTGCATCCGCCAGTATCACAATGTGAATGTTAATGTTGCAGTGCAGACAGACAATGGACTATTTGTTCCTGTCGTCAGGGATGCGAACAAAAAGGGACTATCCGCTATATCTGAGGAGGTTAAGCAGCTGGCCCAAAAAGCCAAGGAGAACAGCTTAAAGCCAGAAGATTATGAGGGAGGGACATTTACAGTGTCGAATTTGGGAGGTCCATTTGGCATCAAACAATTTTGTGCTATCATTAACCCTCCCCAATCAGGCATTCTGGCAATTGGGTCAGCTGAGAAGAGGGTTGTACCTAGTTCCGGGCCCGAGCAATACAAGTTTGCATCTTTCCTATCTGCAACACTAAGCTGTGATCATCGCGTTATTGATGGTGCAATTGGTGCTGAGTGGCTAAAAGCATTCAAAGGCTACATCGAGAATCCAGAGTCAATGTTGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

547

Amino Acids

58.66

Weight (kDa)

6.95

Isoelectric Point (pI)

51.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	116 - 187	9.9e-18	Biotin-requiring enzyme
E3_binding	PF02817	255 - 290	7.1e-15	e3 binding domain
2-oxoacid_dh	PF00198	316 - 547	7.5e-80	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54220 AT1G54220 AT3G13930
fragaria_vesca	FvH4_6g34140 FvH4_6g34140 FvH4_6g34140
malus_domestica	MD09G1188500.v1.1 MD17G1172600.v1.1
prunus_persica	Prupe.3G045200_v2.0.a1 Prupe.3G045200_v2.0.a1
pyrus_communis	pycom09g10560 pycom17g17050
rosa_chinensis	RchiOBHm_Chr2g0144451
rosa_laevigata	RLG00000020082
rosa_multiflora	Rmu_sc0003475.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101300
rosa_rugosa	Rorug02G0386700 Rorug02G0386800
rosa_samantha	Rh2AG439000 Rh2BG449300 Rh2CG426100 Rh2DG459100
rosa_wichuraiana	Rw2G035920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	808
AccB1I	GGYRCC	1 cut(s)	808
AccI	GTMKAC	1 cut(s)	1053
AccII	CGCG	1 cut(s)	1563
AciI	CCGC	5 cut(s)	665, 674, 1114, 1207, 1302
AclWI	GGATC	1 cut(s)	268
AcsI	RAATTY	1 cut(s)	1396
AdeI	CACNNNGTG	1 cut(s)	1222
AfaI	GTAC	5 cut(s)	208, 516, 810, 875, 1494
AfiI	CCNNNNNNNGG	4 cut(s)	752, 943, 1454, 1502
AflIII	ACRYGT	1 cut(s)	1046
AjnI	CCWGG	2 cut(s)	215, 430
AjuI	GAANNNNNNNTTGG	2 cut(s)	1443, 1475
AloI	GAACNNNNNNTCC	2 cut(s)	1248, 1280
Alw21I	GWGCWC	1 cut(s)	444
Alw26I	GTCTC	2 cut(s)	229, 430
AlwI	GGATC	1 cut(s)	268
AlwNI	CAGNNNCTG	2 cut(s)	626, 707
Ama87I	CYCGRG	2 cut(s)	713, 1508
AoxI	GGCC	6 cut(s)	328, 528, 835, 1075, 1329, 1505
ApaI	GGGCCC	2 cut(s)	532, 1509
ApeKI	GCWGC	6 cut(s)	740, 929, 932, 1145, 1148, 1324
ApoI	RAATTY	1 cut(s)	1396
Asp700I	GAANNNNTTC	1 cut(s)	358
Asp718I	GGTACC	1 cut(s)	808
AspLEI	GCGC	1 cut(s)	154
AspS9I	GGNCC	7 cut(s)	528, 529, 836, 1330, 1406, 1505, 1506
AsuC2I	CCSGG	2 cut(s)	839, 1504
AsuHPI	GGTGA	6 cut(s)	205, 360, 428, 446, 565, 644
AvaI	CYCGRG	2 cut(s)	713, 1508
AvaII	GGWCC	1 cut(s)	1406
AxyI	CCTNAGG	1 cut(s)	1173
BaeGI	GKGCMC	2 cut(s)	532, 1509
BaeI	ACNNNNGTAYC	2 cut(s)	1035, 1068
BanI	GGYRCC	1 cut(s)	808
BanII	GRGCYC	2 cut(s)	532, 1509
Bbv12I	GWGCWC	1 cut(s)	444
BbvCI	CCTCAGC	1 cut(s)	795
BbvI	GCAGC	6 cut(s)	752, 916, 944, 1132, 1135, 1336
BccI	CCATC	6 cut(s)	472, 488, 619, 836, 1086, 1565
BciT130I	CCWGG	2 cut(s)	217, 432
BclI	TGATCA	1 cut(s)	1555
BcnI	CCSGG	2 cut(s)	839, 1504
BcoDI	GTCTC	2 cut(s)	229, 430
BfaI	CTAG	3 cut(s)	660, 771, 1497
BfmI	CTRYAG	3 cut(s)	468, 930, 1101
BisI	GCNGC	6 cut(s)	741, 930, 933, 1146, 1149, 1325
BlsI	GCNGC	6 cut(s)	742, 931, 934, 1147, 1150, 1326
BmcAI	AGTACT	1 cut(s)	875
Bme1390I	CCNGG	4 cut(s)	217, 432, 839, 1504
Bme18I	GGWCC	1 cut(s)	1406
BmeT110I	CYCGRG	2 cut(s)	713, 1508
BmgT120I	GGNCC	7 cut(s)	528, 529, 836, 1330, 1406, 1505, 1506
BmiI	GGNNCC	5 cut(s)	529, 530, 810, 1170, 1507
BmrFI	CCNGG	4 cut(s)	217, 432, 839, 1504
BmrI	ACTGGG	1 cut(s)	729
BmsI	GCATC	6 cut(s)	20, 313, 1212, 1270, 1425, 1535
BmuI	ACTGGG	1 cut(s)	729
BplI	GAGNNNNNCTC	2 cut(s)	1065, 1097
BpmI	CTGGAG	1 cut(s)	774
Bpu10I	CCTNAGC	2 cut(s)	795, 921
BpuEI	CTTGAG	1 cut(s)	802
BpuMI	CCSGG	2 cut(s)	839, 1504
BsaBI	GATNNNNATC	2 cut(s)	498, 1121
BsaJI	CCNNGG	2 cut(s)	532, 1341
BsaXI	ACNNNNNCTCC	2 cut(s)	747, 777
Bsc4I	CCNNNNNNNGG	4 cut(s)	752, 943, 1454, 1502
Bse1I	ACTGG	4 cut(s)	701, 735, 948, 1210
Bse21I	CCTNAGG	1 cut(s)	1173
Bse3DI	GCAATG	1 cut(s)	1088
Bse8I	GATNNNNATC	2 cut(s)	498, 1121
BseBI	CCWGG	2 cut(s)	217, 432
BseDI	CCNNGG	2 cut(s)	532, 1341
BseGI	GGATG	6 cut(s)	21, 499, 847, 957, 1203, 1285
BseJI	GATNNNNATC	2 cut(s)	498, 1121
BseLI	CCNNNNNNNGG	4 cut(s)	752, 943, 1454, 1502
BseMI	GCAATG	1 cut(s)	1088
BseMII	CTCAG	7 cut(s)	372, 786, 983, 1187, 1302, 1470, 1578
BseNI	ACTGG	4 cut(s)	701, 735, 948, 1210
BseRI	GAGGAG	3 cut(s)	319, 735, 1328
BseSI	GKGCMC	2 cut(s)	532, 1509
BseXI	GCAGC	6 cut(s)	752, 916, 944, 1132, 1135, 1336
BseYI	CCCAGC	1 cut(s)	937
BsgI	GTGCAG	2 cut(s)	759, 1262
Bsh1236I	CGCG	1 cut(s)	1563
BshFI	GGCC	6 cut(s)	330, 530, 837, 1077, 1331, 1507
BshNI	GGYRCC	1 cut(s)	808
BsiHKAI	GWGCWC	1 cut(s)	444
BsiHKCI	CYCGRG	2 cut(s)	713, 1508
BsiSI	CCGG	2 cut(s)	839, 1503
BslFI	GGGAC	3 cut(s)	309, 1308, 1393
BslI	CCNNNNNNNGG	4 cut(s)	752, 943, 1454, 1502
BsmAI	GTCTC	2 cut(s)	229, 430
BsmFI	GGGAC	3 cut(s)	309, 1308, 1393
BsmI	GAATGC	5 cut(s)	52, 360, 488, 1458, 1601
BsnI	GGCC	6 cut(s)	330, 530, 837, 1077, 1331, 1507
BsoBI	CYCGRG	2 cut(s)	713, 1508
Bsp120I	GGGCCC	2 cut(s)	528, 1505
Bsp1286I	GDGCHC	3 cut(s)	444, 532, 1509
Bsp1407I	TGTACA	1 cut(s)	514
Bsp143I	GATC	4 cut(s)	260, 757, 1129, 1555
BspACI	CCGC	5 cut(s)	665, 674, 1114, 1207, 1302
BspANI	GGCC	6 cut(s)	330, 530, 837, 1077, 1331, 1507
BspCNI	CTCAG	7 cut(s)	373, 787, 982, 1186, 1303, 1471, 1579
BspFNI	CGCG	1 cut(s)	1563
BspLI	GGNNCC	5 cut(s)	529, 530, 810, 1170, 1507
BspMAI	CTGCAG	1 cut(s)	934
BspPI	GGATC	1 cut(s)	268
BspQI	GCTCTTC	1 cut(s)	1164
BspT107I	GGYRCC	1 cut(s)	808
BsrDI	GCAATG	1 cut(s)	1088
BsrGI	TGTACA	1 cut(s)	514
BsrI	ACTGG	4 cut(s)	701, 735, 948, 1210
BssECI	CCNNGG	2 cut(s)	532, 1341
BssMI	GATC	4 cut(s)	260, 757, 1129, 1555
BssT1I	CCWWGG	2 cut(s)	532, 1341
Bst2UI	CCWGG	2 cut(s)	217, 432
Bst4CI	ACNGT	4 cut(s)	472, 568, 1039, 1390
Bst6I	CTCTTC	4 cut(s)	157, 864, 1164, 1478
BstAPI	GCANNNNNTGC	1 cut(s)	1583
BstAUI	TGTACA	1 cut(s)	514
BstC8I	GCNNGC	3 cut(s)	328, 665, 1329
BstDEI	CTNAG	9 cut(s)	381, 795, 921, 969, 1173, 1311, 1479, 1547, 1587
BstF5I	GGATG	6 cut(s)	21, 499, 847, 957, 1203, 1285
BstFNI	CGCG	1 cut(s)	1563
BstHHI	GCGC	1 cut(s)	154
BstKTI	GATC	4 cut(s)	263, 760, 1132, 1558
BstMAI	GTCTC	2 cut(s)	229, 430
BstMBI	GATC	4 cut(s)	260, 757, 1129, 1555
BstMWI	GCNNNNNNNGC	8 cut(s)	673, 695, 917, 926, 938, 1154, 1337, 1583
BstNI	CCWGG	2 cut(s)	217, 432
BstNSI	RCATGY	2 cut(s)	77, 1050
BstSCI	CCNGG	4 cut(s)	215, 430, 837, 1502
BstSFI	CTRYAG	3 cut(s)	468, 930, 1101
BstSLI	GKGCMC	2 cut(s)	532, 1509
BstUI	CGCG	1 cut(s)	1563
BstV1I	GCAGC	6 cut(s)	752, 916, 944, 1132, 1135, 1336
BstXI	CCANNNNNNTGG	1 cut(s)	350
Bsu36I	CCTNAGG	1 cut(s)	1173
BsuRI	GGCC	6 cut(s)	330, 530, 837, 1077, 1331, 1507
BtgZI	GCGATG	1 cut(s)	1544
BtsCI	GGATG	6 cut(s)	21, 499, 847, 957, 1203, 1285
BtsI	GCAGTG	1 cut(s)	1245
BtsIMutI	CAGTG	4 cut(s)	742, 941, 1245, 1395
Cac8I	GCNNGC	3 cut(s)	328, 665, 1329
CaiI	CAGNNNCTG	2 cut(s)	626, 707
CfoI	GCGC	1 cut(s)	154
Cfr13I	GGNCC	7 cut(s)	528, 529, 836, 1330, 1406, 1505, 1506
CseI	GACGC	1 cut(s)	606
Csp6I	GTAC	5 cut(s)	207, 515, 809, 874, 1493
CviAII	CATG	7 cut(s)	68, 74, 143, 285, 487, 1047, 1188
CviQI	GTAC	5 cut(s)	207, 515, 809, 874, 1493
DdeI	CTNAG	9 cut(s)	381, 795, 921, 969, 1173, 1311, 1479, 1547, 1587
DpnI	GATC	4 cut(s)	262, 759, 1131, 1557
DpnII	GATC	4 cut(s)	260, 757, 1129, 1555
DraIII	CACNNNGTG	1 cut(s)	1222
Eam1104I	CTCTTC	4 cut(s)	157, 864, 1164, 1478
EarI	CTCTTC	4 cut(s)	157, 864, 1164, 1478
EciI	GGCGGA	1 cut(s)	1196
Eco130I	CCWWGG	2 cut(s)	532, 1341
Eco147I	AGGCCT	1 cut(s)	330
Eco24I	GRGCYC	2 cut(s)	532, 1509
Eco32I	GATATC	2 cut(s)	500, 1119
Eco47I	GGWCC	1 cut(s)	1406
Eco81I	CCTNAGG	1 cut(s)	1173
Eco88I	CYCGRG	2 cut(s)	713, 1508
EcoO109I	RGGNCCY	1 cut(s)	528
EcoRII	CCWGG	2 cut(s)	215, 430
EcoRV	GATATC	2 cut(s)	500, 1119
EcoT14I	CCWWGG	2 cut(s)	532, 1341
EcoT22I	ATGCAT	2 cut(s)	69, 488
EcoT38I	GRGCYC	2 cut(s)	532, 1509
ErhI	CCWWGG	2 cut(s)	532, 1341
FaeI	CATG	7 cut(s)	71, 77, 146, 288, 490, 1050, 1191
FalI	AAGNNNNNCTT	2 cut(s)	160, 192
FaqI	GGGAC	3 cut(s)	309, 1308, 1393
FatI	CATG	7 cut(s)	67, 73, 142, 284, 486, 1046, 1187
FauI	CCCGC	1 cut(s)	672
FbaI	TGATCA	1 cut(s)	1555
FblI	GTMKAC	1 cut(s)	1053
Fnu4HI	GCNGC	6 cut(s)	741, 930, 933, 1146, 1149, 1325
FokI	GGATG	6 cut(s)	8, 506, 854, 944, 1190, 1292
FriOI	GRGCYC	2 cut(s)	532, 1509
Fsp4HI	GCNGC	6 cut(s)	741, 930, 933, 1146, 1149, 1325
FspBI	CTAG	3 cut(s)	660, 771, 1497
GlaI	GCGC	1 cut(s)	153
GluI	GCNGC	6 cut(s)	741, 930, 933, 1146, 1149, 1325
GsaI	CCCAGC	1 cut(s)	941
GsuI	CTGGAG	1 cut(s)	774
HaeIII	GGCC	6 cut(s)	330, 530, 837, 1077, 1331, 1507
HapII	CCGG	2 cut(s)	839, 1503
HgaI	GACGC	1 cut(s)	606
HhaI	GCGC	1 cut(s)	154
Hin1II	CATG	7 cut(s)	71, 77, 146, 288, 490, 1050, 1191
Hin6I	GCGC	1 cut(s)	152
HinP1I	GCGC	1 cut(s)	152
HincII	GTYRAC	1 cut(s)	1054
HindII	GTYRAC	1 cut(s)	1054
HinfI	GANTC	4 cut(s)	537, 656, 1621, 1628
HpaII	CCGG	2 cut(s)	839, 1503
HphI	GGTGA	6 cut(s)	205, 360, 428, 446, 565, 644
Hpy166II	GTNNAC	1 cut(s)	1054
Hpy188I	TCNGA	7 cut(s)	322, 382, 655, 904, 915, 972, 1312
Hpy188III	TCNNGA	6 cut(s)	258, 713, 753, 993, 1618, 1625
Hpy8I	GTNNAC	1 cut(s)	1054
HpyAV	CCTTC	7 cut(s)	341, 369, 518, 759, 850, 910, 973
HpyCH4III	ACNGT	4 cut(s)	472, 568, 1039, 1390
HpyF10VI	GCNNNNNNNGC	8 cut(s)	673, 695, 917, 926, 938, 1154, 1337, 1583
HpyF3I	CTNAG	9 cut(s)	381, 795, 921, 969, 1173, 1311, 1479, 1547, 1587
Hsp92II	CATG	7 cut(s)	71, 77, 146, 288, 490, 1050, 1191
HspAI	GCGC	1 cut(s)	152
KpnI	GGTACC	1 cut(s)	812
Ksp22I	TGATCA	1 cut(s)	1555
Kzo9I	GATC	4 cut(s)	260, 757, 1129, 1555
LguI	GCTCTTC	1 cut(s)	1164
LmnI	GCTCC	4 cut(s)	55, 715, 748, 916
Lsp1109I	GCAGC	6 cut(s)	752, 916, 944, 1132, 1135, 1336
LweI	GCATC	6 cut(s)	20, 313, 1212, 1270, 1425, 1535
MaeI	CTAG	3 cut(s)	660, 771, 1497
MaeIII	GTNAC	3 cut(s)	562, 703, 982
MalI	GATC	4 cut(s)	262, 759, 1131, 1557
MboI	GATC	4 cut(s)	260, 757, 1129, 1555
MboII	GAAGA	8 cut(s)	174, 418, 584, 593, 881, 1151, 1376, 1495
MfeI	CAATTG	3 cut(s)	299, 1467, 1578
MhlI	GDGCHC	3 cut(s)	444, 532, 1509
MluCI	AATT	8 cut(s)	252, 299, 348, 1084, 1396, 1424, 1467, 1578
MlyI	GAGTC	2 cut(s)	650, 1637
Mph1103I	ATGCAT	2 cut(s)	69, 488
MroXI	GAANNNNTTC	1 cut(s)	358
MseI	TTAA	5 cut(s)	41, 1230, 1320, 1355, 1440
MslI	CAYNNNNRTG	2 cut(s)	72, 141
MspA1I	CMGCKG	2 cut(s)	1327, 1478
MspI	CCGG	2 cut(s)	839, 1503
MspR9I	CCNGG	4 cut(s)	217, 432, 839, 1504
MunI	CAATTG	3 cut(s)	299, 1467, 1578
Mva1269I	GAATGC	5 cut(s)	52, 360, 488, 1458, 1601
MvaI	CCWGG	2 cut(s)	217, 432
MvnI	CGCG	1 cut(s)	1563
MwoI	GCNNNNNNNGC	8 cut(s)	673, 695, 917, 926, 938, 1154, 1337, 1583
NciI	CCSGG	2 cut(s)	839, 1504
NdeII	GATC	4 cut(s)	260, 757, 1129, 1555
NlaIII	CATG	7 cut(s)	71, 77, 146, 288, 490, 1050, 1191
NlaIV	GGNNCC	5 cut(s)	529, 530, 810, 1170, 1507
NsiI	ATGCAT	2 cut(s)	69, 488
NspI	RCATGY	2 cut(s)	77, 1050
PceI	AGGCCT	1 cut(s)	330
PciI	ACATGT	1 cut(s)	1046
PciSI	GCTCTTC	1 cut(s)	1164
PcsI	WCGNNNNNNNCGW	1 cut(s)	1059
PctI	GAATGC	5 cut(s)	52, 360, 488, 1458, 1601
PdmI	GAANNNNTTC	1 cut(s)	358
PfeI	GAWTC	2 cut(s)	537, 1621
PkrI	GCNGC	6 cut(s)	742, 931, 934, 1147, 1150, 1326
PleI	GAGTC	2 cut(s)	650, 1636
PpsI	GAGTC	2 cut(s)	650, 1636
PscI	ACATGT	1 cut(s)	1046
Psp6I	CCWGG	2 cut(s)	215, 430
PspFI	CCCAGC	1 cut(s)	937
PspGI	CCWGG	2 cut(s)	215, 430
PspN4I	GGNNCC	5 cut(s)	529, 530, 810, 1170, 1507
PspOMI	GGGCCC	2 cut(s)	528, 1505
PspPI	GGNCC	7 cut(s)	528, 529, 836, 1330, 1406, 1505, 1506
PsrI	GAACNNNNNNTAC	2 cut(s)	792, 824
PstI	CTGCAG	1 cut(s)	934
PstNI	CAGNNNCTG	2 cut(s)	626, 707
PvuII	CAGCTG	2 cut(s)	1327, 1478
RsaI	GTAC	5 cut(s)	208, 516, 810, 875, 1494
RsaNI	GTAC	5 cut(s)	207, 515, 809, 874, 1493
RseI	CAYNNNNRTG	2 cut(s)	72, 141
SalI	GTCGAC	1 cut(s)	1052
SapI	GCTCTTC	1 cut(s)	1164
SaqAI	TTAA	5 cut(s)	41, 1230, 1320, 1355, 1440
SatI	GCNGC	6 cut(s)	741, 930, 933, 1146, 1149, 1325
Sau3AI	GATC	4 cut(s)	260, 757, 1129, 1555
Sau96I	GGNCC	7 cut(s)	528, 529, 836, 1330, 1406, 1505, 1506
ScaI	AGTACT	1 cut(s)	875
SchI	GAGTC	2 cut(s)	650, 1637
ScrFI	CCNGG	4 cut(s)	217, 432, 839, 1504
SduI	GDGCHC	3 cut(s)	444, 532, 1509
SfaNI	GCATC	6 cut(s)	20, 313, 1212, 1270, 1425, 1535
SfcI	CTRYAG	3 cut(s)	468, 930, 1101
SinI	GGWCC	1 cut(s)	1406
SmiMI	CAYNNNNRTG	2 cut(s)	72, 141
SmlI	CTYRAG	1 cut(s)	817
SmoI	CTYRAG	1 cut(s)	817
Sse9I	AATT	8 cut(s)	252, 299, 348, 1084, 1396, 1424, 1467, 1578
SseBI	AGGCCT	1 cut(s)	330
SsiI	CCGC	5 cut(s)	665, 674, 1114, 1207, 1302
SspI	AATATT	1 cut(s)	391
SspMI	CTAG	3 cut(s)	660, 771, 1497
StuI	AGGCCT	1 cut(s)	330
StyD4I	CCNGG	4 cut(s)	215, 430, 837, 1502
StyI	CCWWGG	2 cut(s)	532, 1341
TaaI	ACNGT	4 cut(s)	472, 568, 1039, 1390
TaqI	TCGA	3 cut(s)	1053, 1394, 1617
TasI	AATT	8 cut(s)	252, 299, 348, 1084, 1396, 1424, 1467, 1578
TatI	WGTACW	2 cut(s)	514, 873
TfiI	GAWTC	2 cut(s)	537, 1621
Tru1I	TTAA	5 cut(s)	41, 1230, 1320, 1355, 1440
Tru9I	TTAA	5 cut(s)	41, 1230, 1320, 1355, 1440
TscAI	CASTG	4 cut(s)	742, 948, 1245, 1395
TseI	GCWGC	6 cut(s)	740, 929, 932, 1145, 1148, 1324
TspDTI	ATGAA	5 cut(s)	84, 125, 251, 273, 1077
TspGWI	ACGGA	2 cut(s)	968, 991
TspRI	CASTG	4 cut(s)	742, 948, 1245, 1395
VpaK11BI	GGWCC	1 cut(s)	1406
XapI	RAATTY	1 cut(s)	1396
XceI	RCATGY	2 cut(s)	77, 1050
XmiI	GTMKAC	1 cut(s)	1053
XmnI	GAANNNNTTC	1 cut(s)	358
XspI	CTAG	3 cut(s)	660, 771, 1497
ZrmI	AGTACT	1 cut(s)	875
Zsp2I	ATGCAT	2 cut(s)	69, 488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.