Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008560

Physical Location & Seq

Genomic Coordinates Reverse (-)

17796785 .. 17803049

Gene Structure 6265 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1172600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGACTTACGCATCTCGTATCATCCACCACTCCAAAAAGTTACGGAATGCTCCTAACTTACTATGGCAAGAGCAAGCACACTTGGTTCGTTGGTTTTCACATGATGTTCAACCCTCTAGTGATATATTGAGCGTTCATCGCCACAGTTATGTGCCTTCAGAAAGAGCATGCGGTTCTAGGTCTTTCAGCAGTAGCACTAAGTCAGTGTCAGGTACATTTACCAGGGATATCCCAATGACAAGGAAACTAGGAAGTTCAATTTCTGGATCGGTATTCAACAAACAGCTTTCATGTATTCAGGTGCAGTTGAGCAGAGGCTTGTCATCTGATGCAGGCCTCCCTCCACACCAAGAAATTGGGATGCCTTCTCTTTCACCCACTATGTCTGAGGGTAATATTGCTAGGTGGCTGAAGAAAGAGGGTGATAAAGTTTCTCCTGGTGAAGTTCTATGTGAAGTTGAAACTGATAAAGCTACAGTGGAGATGGAATGCATGGAAGATGGTTATCTTGCCAAGATTCTACGACCAGATGGGACCCAAGGGATCAAAGTTGGAGAGGTGATTGCAATAACTGTTGAAGAGGAGGATGATATTGCAAAATTTAAAGATTATACACCATCGGCACCTGCAGCAGCTAAAGAGGCTTCTGAGCCTACCCTGCCCAAAAAAGAGGTGGTTGCAGAACCAGTGCCAGAGCCAAAGGTTTCAAAGCCCAGTGCAGCTCCTGCTGCTACCCCTTCTGGAGATCGCATATTCGCAAGTCCTTTGGCAAGAAGTTTGGCTGAAGAGCACAAGGTTCCCCTCTCAAGTATCAAAGGAACAGGTCCTGATGGAAGCATTGTGAAGGCTGATGTTGAAGAGTATTTGGCTTCTCATGGGAAGGAAGCTCCTAAAGCTAAGGGTGCAGCACCTGCAGCATTAGATTATACCGATATCGCCCATTCTCAGATACGGAAGATCACAGCTTCAAGATTATTGTTCTCAAAGCAAACAATTCCTCATTATTATTTGACGGTAGATACATGCGTCGATAGACTAATGGATTTGAGAGGCCAACTCAATGCATTACAAGAGGCATCAGGTGGGAAGCGGATATCTGTTAATGATCTCGTAATTAAGGCTGCTGCTTTGGCTCTTCAAAAGGTTCCTCAATGCAACAGTTCATGGACAGATGACTACATCCGCCAGTTTCACAACGTGAACATTAATGTTGCTGTGCAGACAGAAAATGGACTGTTTGTACCCGTCATTAGGGACGCAAACAAGAAGGGACTATCCAGCATAGCCGAGGAGGTCAGGCAGCTGGCCCAAAAAGCCAAAGAAAACAGCTTAAAGCCAGAAGATTACGAGGGAGGCACATTTACGGTGACAAATTTGGGAGGTCCATTCGGCATCAAACAATTTTGTGCCATTATTAACCCTCCTCAATCAGGCATTCTTGCAGTTGGATCAGCTGAGAAGAGGGTTGTACCTAGTTCTGGGCCCGAACTATACAAGTTTGTATCCTATATGTCTGTAACGCTAAGCTGTGATCATCGTGTTATCGATGGTGCAATTGGTGCGGAATGGCTAAAGGCATTCAAAGGCTACGTTGAGAATCCTGAGTCAATGTTGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

540

Amino Acids

58.42

Weight (kDa)

6.9

Isoelectric Point (pI)

51.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	118 - 189	1e-18	Biotin-requiring enzyme
E3_binding	PF02817	251 - 286	3.1e-15	e3 binding domain
2-oxoacid_dh	PF00198	308 - 539	3.4e-78	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54220 AT1G54220 AT3G13930
fragaria_vesca	FvH4_6g34140 FvH4_6g34140 FvH4_6g34140
malus_domestica	MD09G1188500.v1.1 MD17G1172600.v1.1
prunus_persica	Prupe.3G045200_v2.0.a1 Prupe.3G045200_v2.0.a1
pyrus_communis	pycom09g10560 pycom17g17050
rosa_chinensis	RchiOBHm_Chr2g0144451
rosa_laevigata	RLG00000020082
rosa_multiflora	Rmu_sc0003475.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101300
rosa_rugosa	Rorug02G0386700 Rorug02G0386800
rosa_samantha	Rh2AG439000 Rh2BG449300 Rh2CG426100 Rh2DG459100
rosa_wichuraiana	Rw2G035920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	634, 919
Acc36I	ACCTGC	2 cut(s)	634, 919
AccB1I	GGYRCC	1 cut(s)	622
AciI	CCGC	4 cut(s)	171, 1090, 1183, 1562
AclWI	GGATC	3 cut(s)	274, 551, 1456
AcsI	RAATTY	2 cut(s)	599, 1372
AcuI	CTGAAG	3 cut(s)	141, 431, 804
AdeI	CACNNNGTG	1 cut(s)	1198
AfaI	GTAC	3 cut(s)	214, 1242, 1470
AfiI	CCNNNNNNNGG	3 cut(s)	1251, 1430, 1478
AjnI	CCWGG	2 cut(s)	221, 436
Alw21I	GWGCWC	1 cut(s)	792
AlwI	GGATC	3 cut(s)	274, 551, 1456
AlwNI	CAGNNNCTG	3 cut(s)	725, 827, 911
AoxI	GGCC	4 cut(s)	334, 1051, 1305, 1481
ApaI	GGGCCC	1 cut(s)	1485
ApeKI	GCWGC	9 cut(s)	629, 632, 719, 728, 905, 914, 1121, 1124, 1300
ApoI	RAATTY	2 cut(s)	599, 1372
AseI	ATTAAT	1 cut(s)	1206
AspS9I	GGNCC	6 cut(s)	534, 824, 1306, 1382, 1481, 1482
AsuHPI	GGTGA	5 cut(s)	366, 434, 452, 571, 1378
AvaII	GGWCC	3 cut(s)	534, 824, 1382
BaeGI	GKGCMC	1 cut(s)	1485
BaeI	ACNNNNGTAYC	2 cut(s)	1011, 1044
BanI	GGYRCC	1 cut(s)	622
BanII	GRGCYC	1 cut(s)	1485
Bbv12I	GWGCWC	1 cut(s)	792
BbvI	GCAGC	9 cut(s)	641, 644, 715, 731, 917, 926, 1108, 1111, 1312
BccI	CCATC	6 cut(s)	478, 494, 524, 625, 824, 1541
BciT130I	CCWGG	2 cut(s)	223, 438
BciVI	GTATCC	1 cut(s)	1513
BclI	TGATCA	1 cut(s)	1531
BfaI	CTAG	5 cut(s)	117, 177, 248, 402, 1473
BfmI	CTRYAG	3 cut(s)	474, 627, 912
BfuAI	ACCTGC	2 cut(s)	634, 919
BfuI	GTATCC	1 cut(s)	1513
BisI	GCNGC	9 cut(s)	630, 633, 720, 729, 906, 915, 1122, 1125, 1301
BlpI	GCTNAGC	1 cut(s)	1523
BlsI	GCNGC	9 cut(s)	631, 634, 721, 730, 907, 916, 1123, 1126, 1302
Bme1390I	CCNGG	2 cut(s)	223, 438
Bme18I	GGWCC	3 cut(s)	534, 824, 1382
BmgT120I	GGNCC	6 cut(s)	534, 824, 1306, 1382, 1481, 1482
BmiI	GGNNCC	6 cut(s)	535, 536, 624, 798, 1146, 1483
BmrFI	CCNGG	2 cut(s)	223, 438
BmrI	ACTGGG	1 cut(s)	708
BmsI	GCATC	5 cut(s)	20, 319, 351, 1085, 1401
BmuI	ACTGGG	1 cut(s)	708
BplI	GAGNNNNNCTC	2 cut(s)	1041, 1073
BpmI	CTGGAG	1 cut(s)	762
Bpu10I	CCTNAGC	1 cut(s)	897
Bpu1102I	GCTNAGC	1 cut(s)	1523
BpuEI	CTTGAG	1 cut(s)	790
Bsa29I	ATCGAT	1 cut(s)	1545
BsaBI	GATNNNNATC	1 cut(s)	504
BsaJI	CCNNGG	3 cut(s)	222, 538, 1287
Bsc4I	CCNNNNNNNGG	3 cut(s)	1251, 1430, 1478
Bse1I	ACTGG	3 cut(s)	686, 714, 1186
Bse8I	GATNNNNATC	1 cut(s)	504
BseBI	CCWGG	2 cut(s)	223, 438
BseCI	ATCGAT	1 cut(s)	1545
BseDI	CCNNGG	3 cut(s)	222, 538, 1287
BseGI	GGATG	4 cut(s)	21, 366, 592, 1179
BseJI	GATNNNNATC	1 cut(s)	504
BseLI	CCNNNNNNNGG	3 cut(s)	1251, 1430, 1478
BseMII	CTCAG	5 cut(s)	378, 639, 959, 1446, 1593
BseNI	ACTGG	3 cut(s)	686, 714, 1186
BseRI	GAGGAG	3 cut(s)	596, 1304, 1413
BseSI	GKGCMC	1 cut(s)	1485
BseXI	GCAGC	9 cut(s)	641, 644, 715, 731, 917, 926, 1108, 1111, 1312
BsgI	GTGCAG	4 cut(s)	323, 738, 924, 1238
BshFI	GGCC	4 cut(s)	336, 1053, 1307, 1483
BshNI	GGYRCC	1 cut(s)	622
BshVI	ATCGAT	1 cut(s)	1545
BsiHKAI	GWGCWC	1 cut(s)	792
BslFI	GGGAC	3 cut(s)	547, 1268, 1284
BslI	CCNNNNNNNGG	3 cut(s)	1251, 1430, 1478
BsmFI	GGGAC	3 cut(s)	547, 1268, 1284
BsmI	GAATGC	4 cut(s)	52, 494, 1434, 1577
BsnI	GGCC	4 cut(s)	336, 1053, 1307, 1483
Bsp120I	GGGCCC	1 cut(s)	1481
Bsp1286I	GDGCHC	2 cut(s)	792, 1485
Bsp143I	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
Bsp1720I	GCTNAGC	1 cut(s)	1523
BspACI	CCGC	4 cut(s)	171, 1090, 1183, 1562
BspANI	GGCC	4 cut(s)	336, 1053, 1307, 1483
BspCNI	CTCAG	5 cut(s)	379, 640, 958, 1447, 1594
BspDI	ATCGAT	1 cut(s)	1545
BspLI	GGNNCC	6 cut(s)	535, 536, 624, 798, 1146, 1483
BspMAI	CTGCAG	2 cut(s)	631, 916
BspMI	ACCTGC	2 cut(s)	634, 919
BspPI	GGATC	3 cut(s)	274, 551, 1456
BspQI	GCTCTTC	2 cut(s)	780, 1140
BspT107I	GGYRCC	1 cut(s)	622
BsrI	ACTGG	3 cut(s)	686, 714, 1186
BssECI	CCNNGG	3 cut(s)	222, 538, 1287
BssMI	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
BssT1I	CCWWGG	1 cut(s)	538
Bst2UI	CCWGG	2 cut(s)	223, 438
Bst4CI	ACNGT	7 cut(s)	146, 478, 574, 1015, 1160, 1236, 1366
Bst6I	CTCTTC	5 cut(s)	573, 780, 852, 1140, 1454
BstAPI	GCANNNNNTGC	3 cut(s)	725, 911, 1559
BstC8I	GCNNGC	4 cut(s)	75, 169, 334, 1305
BstDEI	CTNAG	8 cut(s)	198, 387, 648, 897, 945, 1455, 1523, 1602
BstENI	CCTNNNNNAGG	1 cut(s)	1428
BstF5I	GGATG	4 cut(s)	21, 366, 592, 1179
BstKTI	GATC	7 cut(s)	269, 546, 748, 960, 1108, 1451, 1534
BstMBI	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
BstNI	CCWGG	2 cut(s)	223, 438
BstNSI	RCATGY	2 cut(s)	171, 1026
BstSCI	CCNGG	2 cut(s)	221, 436
BstSFI	CTRYAG	3 cut(s)	474, 627, 912
BstSLI	GKGCMC	1 cut(s)	1485
BstV1I	GCAGC	9 cut(s)	641, 644, 715, 731, 917, 926, 1108, 1111, 1312
BstXI	CCANNNNNNTGG	1 cut(s)	356
Bsu15I	ATCGAT	1 cut(s)	1545
BsuI	GTATCC	1 cut(s)	1513
BsuRI	GGCC	4 cut(s)	336, 1053, 1307, 1483
BsuTUI	ATCGAT	1 cut(s)	1545
BtgZI	GCGATG	1 cut(s)	122
BtsCI	GGATG	4 cut(s)	21, 366, 592, 1179
BtsIMutI	CAGTG	4 cut(s)	210, 483, 693, 721
BveI	ACCTGC	2 cut(s)	634, 919
Cac8I	GCNNGC	4 cut(s)	75, 169, 334, 1305
CaiI	CAGNNNCTG	3 cut(s)	725, 827, 911
Cfr13I	GGNCC	6 cut(s)	534, 824, 1306, 1382, 1481, 1482
ClaI	ATCGAT	1 cut(s)	1545
CseI	GACGC	2 cut(s)	1015, 1265
Csp6I	GTAC	3 cut(s)	213, 1241, 1469
CviAII	CATG	7 cut(s)	101, 168, 291, 493, 875, 1023, 1164
CviQI	GTAC	3 cut(s)	213, 1241, 1469
DdeI	CTNAG	8 cut(s)	198, 387, 648, 897, 945, 1455, 1523, 1602
DpnI	GATC	7 cut(s)	268, 545, 747, 959, 1107, 1450, 1533
DpnII	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
DraI	TTTAAA	1 cut(s)	604
DraIII	CACNNNGTG	1 cut(s)	1198
Eam1104I	CTCTTC	5 cut(s)	573, 780, 852, 1140, 1454
EarI	CTCTTC	5 cut(s)	573, 780, 852, 1140, 1454
EciI	GGCGGA	1 cut(s)	1172
Eco130I	CCWWGG	1 cut(s)	538
Eco147I	AGGCCT	1 cut(s)	336
Eco24I	GRGCYC	1 cut(s)	1485
Eco32I	GATATC	3 cut(s)	229, 934, 1095
Eco47I	GGWCC	3 cut(s)	534, 824, 1382
Eco57I	CTGAAG	3 cut(s)	141, 431, 804
EcoNI	CCTNNNNNAGG	1 cut(s)	1428
EcoO109I	RGGNCCY	2 cut(s)	534, 824
EcoRII	CCWGG	2 cut(s)	221, 436
EcoRV	GATATC	3 cut(s)	229, 934, 1095
EcoT14I	CCWWGG	1 cut(s)	538
EcoT22I	ATGCAT	2 cut(s)	494, 1066
EcoT38I	GRGCYC	1 cut(s)	1485
ErhI	CCWWGG	1 cut(s)	538
FaeI	CATG	7 cut(s)	104, 171, 294, 496, 878, 1026, 1167
FaqI	GGGAC	3 cut(s)	547, 1268, 1284
FatI	CATG	7 cut(s)	100, 167, 290, 492, 874, 1022, 1163
FbaI	TGATCA	1 cut(s)	1531
Fnu4HI	GCNGC	9 cut(s)	630, 633, 720, 729, 906, 915, 1122, 1125, 1301
FokI	GGATG	4 cut(s)	8, 373, 599, 1166
FriOI	GRGCYC	1 cut(s)	1485
Fsp4HI	GCNGC	9 cut(s)	630, 633, 720, 729, 906, 915, 1122, 1125, 1301
FspBI	CTAG	5 cut(s)	117, 177, 248, 402, 1473
GluI	GCNGC	9 cut(s)	630, 633, 720, 729, 906, 915, 1122, 1125, 1301
GsuI	CTGGAG	1 cut(s)	762
HaeIII	GGCC	4 cut(s)	336, 1053, 1307, 1483
HgaI	GACGC	2 cut(s)	1015, 1265
Hin1II	CATG	7 cut(s)	104, 171, 294, 496, 878, 1026, 1167
HinfI	GANTC	3 cut(s)	517, 1597, 1604
HphI	GGTGA	5 cut(s)	366, 434, 452, 571, 1378
Hpy166II	GTNNAC	1 cut(s)	1201
Hpy188I	TCNGA	5 cut(s)	160, 328, 388, 649, 948
Hpy188III	TCNNGA	5 cut(s)	264, 741, 827, 969, 1601
Hpy8I	GTNNAC	1 cut(s)	1201
Hpy99I	CGWCG	1 cut(s)	1031
HpyAV	CCTTC	6 cut(s)	165, 375, 747, 838, 874, 1261
HpyCH4III	ACNGT	7 cut(s)	146, 478, 574, 1015, 1160, 1236, 1366
HpyCH4IV	ACGT	2 cut(s)	1197, 1590
HpyF3I	CTNAG	8 cut(s)	198, 387, 648, 897, 945, 1455, 1523, 1602
HpySE526I	ACGT	2 cut(s)	1197, 1590
Hsp92II	CATG	7 cut(s)	104, 171, 294, 496, 878, 1026, 1167
KflI	GGGWCCC	1 cut(s)	534
Ksp22I	TGATCA	1 cut(s)	1531
Kzo9I	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
LguI	GCTCTTC	2 cut(s)	780, 1140
LmnI	GCTCC	3 cut(s)	55, 727, 892
Lsp1109I	GCAGC	9 cut(s)	641, 644, 715, 731, 917, 926, 1108, 1111, 1312
LweI	GCATC	5 cut(s)	20, 319, 351, 1085, 1401
MaeI	CTAG	5 cut(s)	117, 177, 248, 402, 1473
MaeII	ACGT	2 cut(s)	1197, 1590
MaeIII	GTNAC	3 cut(s)	39, 1366, 1516
MalI	GATC	7 cut(s)	268, 545, 747, 959, 1107, 1450, 1533
MboI	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
MboII	GAAGA	9 cut(s)	424, 509, 590, 797, 869, 967, 1127, 1352, 1471
MfeI	CAATTG	1 cut(s)	1554
MhlI	GDGCHC	2 cut(s)	792, 1485
MluCI	AATT	8 cut(s)	258, 354, 599, 993, 1113, 1372, 1400, 1554
MlyI	GAGTC	1 cut(s)	1613
MmeI	TCCRAC	2 cut(s)	532, 1426
Mph1103I	ATGCAT	2 cut(s)	494, 1066
MseI	TTAA	6 cut(s)	603, 1101, 1116, 1206, 1331, 1416
MslI	CAYNNNNRTG	1 cut(s)	147
MspA1I	CMGCKG	2 cut(s)	1303, 1454
MspR9I	CCNGG	2 cut(s)	223, 438
MunI	CAATTG	1 cut(s)	1554
Mva1269I	GAATGC	4 cut(s)	52, 494, 1434, 1577
MvaI	CCWGG	2 cut(s)	223, 438
NdeII	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
NlaIII	CATG	7 cut(s)	104, 171, 294, 496, 878, 1026, 1167
NlaIV	GGNNCC	6 cut(s)	535, 536, 624, 798, 1146, 1483
NmeAIII	GCCGAG	1 cut(s)	1312
NmuCI	GTSAC	1 cut(s)	1366
NsiI	ATGCAT	2 cut(s)	494, 1066
NspI	RCATGY	2 cut(s)	171, 1026
PaeI	GCATGC	1 cut(s)	171
PaqCI	CACCTGC	2 cut(s)	634, 919
PceI	AGGCCT	1 cut(s)	336
PciSI	GCTCTTC	2 cut(s)	780, 1140
PctI	GAATGC	4 cut(s)	52, 494, 1434, 1577
PfeI	GAWTC	2 cut(s)	517, 1597
PkrI	GCNGC	9 cut(s)	631, 634, 721, 730, 907, 916, 1123, 1126, 1302
PleI	GAGTC	1 cut(s)	1612
PpsI	GAGTC	1 cut(s)	1612
PpuMI	RGGWCCY	2 cut(s)	534, 824
PshBI	ATTAAT	1 cut(s)	1206
Psp5II	RGGWCCY	2 cut(s)	534, 824
Psp6I	CCWGG	2 cut(s)	221, 436
PspGI	CCWGG	2 cut(s)	221, 436
PspN4I	GGNNCC	6 cut(s)	535, 536, 624, 798, 1146, 1483
PspOMI	GGGCCC	1 cut(s)	1481
PspPI	GGNCC	6 cut(s)	534, 824, 1306, 1382, 1481, 1482
PspPPI	RGGWCCY	2 cut(s)	534, 824
PstI	CTGCAG	2 cut(s)	631, 916
PstNI	CAGNNNCTG	3 cut(s)	725, 827, 911
PvuII	CAGCTG	2 cut(s)	1303, 1454
RsaI	GTAC	3 cut(s)	214, 1242, 1470
RsaNI	GTAC	3 cut(s)	213, 1241, 1469
RseI	CAYNNNNRTG	1 cut(s)	147
SapI	GCTCTTC	2 cut(s)	780, 1140
SaqAI	TTAA	6 cut(s)	603, 1101, 1116, 1206, 1331, 1416
SatI	GCNGC	9 cut(s)	630, 633, 720, 729, 906, 915, 1122, 1125, 1301
Sau3AI	GATC	7 cut(s)	266, 543, 745, 957, 1105, 1448, 1531
Sau96I	GGNCC	6 cut(s)	534, 824, 1306, 1382, 1481, 1482
SchI	GAGTC	1 cut(s)	1613
ScrFI	CCNGG	2 cut(s)	223, 438
SduI	GDGCHC	2 cut(s)	792, 1485
SfaNI	GCATC	5 cut(s)	20, 319, 351, 1085, 1401
SfcI	CTRYAG	3 cut(s)	474, 627, 912
SinI	GGWCC	3 cut(s)	534, 824, 1382
SmiMI	CAYNNNNRTG	1 cut(s)	147
SmlI	CTYRAG	1 cut(s)	805
SmoI	CTYRAG	1 cut(s)	805
SphI	GCATGC	1 cut(s)	171
Sse9I	AATT	8 cut(s)	258, 354, 599, 993, 1113, 1372, 1400, 1554
SseBI	AGGCCT	1 cut(s)	336
SsiI	CCGC	4 cut(s)	171, 1090, 1183, 1562
SspI	AATATT	1 cut(s)	397
SspMI	CTAG	5 cut(s)	117, 177, 248, 402, 1473
StuI	AGGCCT	1 cut(s)	336
StyD4I	CCNGG	2 cut(s)	221, 436
StyI	CCWWGG	1 cut(s)	538
TaaI	ACNGT	7 cut(s)	146, 478, 574, 1015, 1160, 1236, 1366
TaiI	ACGT	2 cut(s)	1200, 1593
TaqI	TCGA	2 cut(s)	1029, 1545
TasI	AATT	8 cut(s)	258, 354, 599, 993, 1113, 1372, 1400, 1554
TfiI	GAWTC	2 cut(s)	517, 1597
Tru1I	TTAA	6 cut(s)	603, 1101, 1116, 1206, 1331, 1416
Tru9I	TTAA	6 cut(s)	603, 1101, 1116, 1206, 1331, 1416
TscAI	CASTG	4 cut(s)	210, 483, 693, 721
TseFI	GTSAC	1 cut(s)	1366
TseI	GCWGC	9 cut(s)	629, 632, 719, 728, 905, 914, 1121, 1124, 1300
Tsp45I	GTSAC	1 cut(s)	1366
TspDTI	ATGAA	3 cut(s)	125, 279, 1152
TspGWI	ACGGA	2 cut(s)	58, 967
TspRI	CASTG	4 cut(s)	210, 483, 693, 721
VpaK11BI	GGWCC	3 cut(s)	534, 824, 1382
VspI	ATTAAT	1 cut(s)	1206
XagI	CCTNNNNNAGG	1 cut(s)	1428
XapI	RAATTY	2 cut(s)	599, 1372
XceI	RCATGY	2 cut(s)	171, 1026
XcmI	CCANNNNNNNNNTGG	1 cut(s)	670
XspI	CTAG	5 cut(s)	117, 177, 248, 402, 1473
Zsp2I	ATGCAT	2 cut(s)	494, 1066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.