Rorug02G0386700

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008560
Physical Location & Seq
Reverse (-)
49331610 .. 49332576
967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0386700.1

Sequence Viewer

Length: 684 bp
ATGCCACTCAAATCTCCCACTGTAGATGACTACTTGGGCATCTACAACATCATGTTTTGGACCCTTACTCTCATTGGGGTCGTCAAATATGCCACCATTGCTCTTAGAGTGGATGATGATGGTGAAGGTGGAACTTTCTCCCTATATTCATTACTTTGCAGGAGTATGAATATCACTGAAACTCTTTCCTCGAGACCTTCAAATCCAAAGTCATCAAGTCTTCCACGCTCTATCTATGATGACACTCAAAATCAGACTAGCATGGTAAAAGTTTTCAAGGCAAGTATAGTTGCCAGAAGGTTATTGCTTTTTATTGCTATGTTAGGCATGTGCATGCTTATTGGAGATGGTATTCTCACTCCTGCAATATCCGTTTGGTCTGCAATAGATGGAATAAGAGTACCTTTTCCATCTGTTAGTTCATCTGTGGTGAAAGTGCTTTCTACAGCTGTGCTTGTCATTCTTTTCATGGTACAGAAATTTGGTACATCTCGAGTGAGCTTCATGTTTTCCCCGATTATGGGAGCATGGAGTTTGAGTATCATGCTCGTTGGAATATATAACATCATTCGACACTACCCGAGCATATTTAAGGCTGTATCACCACATTATATCTTCCGCTTCTTTTGGAGAAACGGCAAGGAAGGCTGGCTGATGCTTGGTGGCACTGTCCTTTGTTGGTAA

Protein Analysis

227

Amino Acids

25.31

Weight (kDa)

9.57

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 5 - 226 6.1e-59 K+ potassium transporter integral membrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 619
AcsI RAATTY 1 cut(s) 479
AfaI GTAC 3 cut(s) 402, 474, 487
AfiI CCNNNNNNNGG 2 cut(s) 520, 521
AgsI TTSAA 2 cut(s) 201, 277
AluBI AGCT 2 cut(s) 449, 501
AluI AGCT 2 cut(s) 449, 501
Alw26I GTCTC 1 cut(s) 187
Ama87I CYCGRG 3 cut(s) 190, 492, 580
ApoI RAATTY 1 cut(s) 479
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 134, 442, 594
AvaI CYCGRG 3 cut(s) 190, 492, 580
AvaII GGWCC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 212
BccI CCATC 4 cut(s) 113, 341, 383, 418
BceAI ACGGC 1 cut(s) 652
BcoDI GTCTC 1 cut(s) 187
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 2 cut(s) 21, 444
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 3 cut(s) 190, 492, 580
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 62
BmsI GCATC 2 cut(s) 48, 645
BpiI GAAGAC 1 cut(s) 212
BsaI GGTCTC 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 2 cut(s) 520, 521
Bse3DI GCAATG 1 cut(s) 96
BseGI GGATG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 520, 521
BseMI GCAATG 1 cut(s) 96
BsiHKCI CYCGRG 3 cut(s) 190, 492, 580
BslI CCNNNNNNNGG 2 cut(s) 520, 521
BsmAI GTCTC 1 cut(s) 187
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 3 cut(s) 190, 492, 580
BspACI CCGC 1 cut(s) 619
BspLI GGNNCC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 22, 670
BstC8I GCNNGC 2 cut(s) 335, 650
BstDEI CTNAG 1 cut(s) 104
BstF5I GGATG 1 cut(s) 118
BstMAI GTCTC 1 cut(s) 187
BstMWI GCNNNNNNNGC 2 cut(s) 98, 645
BstNSI RCATGY 2 cut(s) 331, 337
BstSFI CTRYAG 2 cut(s) 21, 444
BstV2I GAAGAC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 3 cut(s) 18, 174, 666
Cac8I GCNNGC 2 cut(s) 335, 650
Cfr13I GGNCC 1 cut(s) 60
Csp6I GTAC 3 cut(s) 401, 473, 486
CviAII CATG 8 cut(s) 52, 262, 328, 334, 469, 505, 528, 544
CviJI RGCY 5 cut(s) 449, 501, 596, 648, 652
CviKI_1 RGCY 5 cut(s) 449, 501, 596, 648, 652
CviQI GTAC 3 cut(s) 401, 473, 486
DdeI CTNAG 1 cut(s) 104
Eco31I GGTCTC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 60
Eco88I CYCGRG 3 cut(s) 190, 492, 580
FaeI CATG 8 cut(s) 55, 265, 331, 337, 472, 508, 531, 547
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FatI CATG 8 cut(s) 51, 261, 327, 333, 468, 504, 527, 543
FokI GGATG 1 cut(s) 125
FspBI CTAG 1 cut(s) 258
Hin1II CATG 8 cut(s) 55, 265, 331, 337, 472, 508, 531, 547
HphI GGTGA 3 cut(s) 134, 442, 594
Hpy188I TCNGA 1 cut(s) 255
Hpy188III TCNNGA 2 cut(s) 192, 492
HpyAV CCTTC 4 cut(s) 119, 207, 291, 638
HpyCH4III ACNGT 2 cut(s) 22, 670
HpyCH4V TGCA 4 cut(s) 159, 333, 365, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 645
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 8 cut(s) 55, 265, 331, 337, 472, 508, 531, 547
LmnI GCTCC 1 cut(s) 524
LpnPI CCDG 4 cut(s) 145, 307, 375, 634
LweI GCATC 2 cut(s) 48, 645
MaeI CTAG 1 cut(s) 258
MboII GAAGA 2 cut(s) 212, 607
MluCI AATT 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 1 cut(s) 199
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 449
MwoI GCNNNNNNNGC 2 cut(s) 98, 645
NlaIII CATG 8 cut(s) 55, 265, 331, 337, 472, 508, 531, 547
NlaIV GGNNCC 1 cut(s) 62
NspI RCATGY 2 cut(s) 331, 337
PaeI GCATGC 1 cut(s) 337
PaeR7I CTCGAG 2 cut(s) 190, 492
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 3 cut(s) 402, 474, 487
RsaNI GTAC 3 cut(s) 401, 473, 486
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 1 cut(s) 591
Sau96I GGNCC 1 cut(s) 60
SetI ASST 6 cut(s) 130, 199, 302, 406, 451, 503
SfaNI GCATC 2 cut(s) 48, 645
SfcI CTRYAG 2 cut(s) 21, 444
Sfr274I CTCGAG 2 cut(s) 190, 492
SinI GGWCC 1 cut(s) 60
SlaI CTCGAG 2 cut(s) 190, 492
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 2 cut(s) 190, 492
SmoI CTYRAG 2 cut(s) 190, 492
SphI GCATGC 1 cut(s) 337
Sse9I AATT 1 cut(s) 479
SsiI CCGC 1 cut(s) 619
SspMI CTAG 1 cut(s) 258
TaaI ACNGT 2 cut(s) 22, 670
TaqI TCGA 3 cut(s) 191, 493, 571
TasI AATT 1 cut(s) 479
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 3 cut(s) 25, 181, 673
TspDTI ATGAA 5 cut(s) 138, 182, 411, 457, 493
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 3 cut(s) 25, 181, 673
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 1 cut(s) 479
XceI RCATGY 2 cut(s) 331, 337
XhoI CTCGAG 2 cut(s) 190, 492
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.