Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008560

Physical Location & Seq

Genomic Coordinates Reverse (-)

16478338 .. 16484718

Gene Structure 6381 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1188500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGACTTACGCATCTCGTATCATCCACCATTCGAAAAGGTTACAGAATGCCCCCAACTTAGTACGGCAAGAGCAAGCACTCTTGGTTCGTTGGTTTTCACATGATGCTCAACCCTCTACTGATATCCTGAAGGCTCGTCGCCCCAGTTGTGTGCCTTCAGAAAGAGCAAGAGGTTCTAGGTCTTTCAGCAGTAGCACTAAATCAGTGTCTGGTACATTTACCAGGGATATCTCAATGACAAGGAAACTAGGAAGTTCAGTTTCTGAATCAGGATTCAACAAACGGCTTTCATGTATGCAGGTGCAATTGAGCAGAGGCTTTTCATCTGATGCTGGCCTTCCTCCACACCAAGAAATTGGAATGCCTTCTCTTTCACCCACTATGTCTGAGGGTAATATTGCTAGGTGGCTGAAGAAAGAGGGTGACAAAGTTTCTCCTGGTGAAGTTCTTTGTGAAGTTGAAACTGATAAAGCTACAGTGGAGATGGAATGCATGGAAGATGGTTATCTTGCCAAGATTGTACGACCAGATGGGACCCAAGGGATCAAAGTTGGAGAGGTGATTGCAATTACCGTTGAAGAGGAGGATGATATTGCAAAATTTAAAGATTACACACCATCGGCATCTGGTGCCAGTGCTTCTGCAGCTAAAGAGTCATCTGAGCCTACTCCGCCCAAAAAAGAGGTGGTTGCAGAACCAGTTACTTCCCCAGAGCCAAAGGTATCAAAGCCCAGTGCAGCTCCTGTTGCTTCCCCTTCTGGAGATCGCATATTTGCAAGTCCTCTGGCAAGAAGTTTGGCCGAAGAGCACAAGGTTCCCCTCTCAAGTATCAAAGGAACAGGTCCTGATGGAAGCATTGTGAAGGCTGATGTTGAAGAGTATTTGGCTTCTCGTGGGAAGGAAGCCCCTAAAGCTAAGGGTGCAGTACCTGCAGCGTTGGATTACACCGATATCCCCCATTCTCAGATACGGAAGATCACAGCTTCGAGATTATTATTCTCAAAGCAAACAATTCCTCATTATTATTTGACAGTAGATACATGTGTTGATAGACTCATGGATTTGAGAGGCCAACTCAATGCACTACAAGAGGCATCAGGTGGGAAGCGGATATCTGTTAATGATCTCGTAATTAAGGCTGCTGCTTTGGCTCTTCAAAAGGTTCCTCAATGCAATAGTTCATGGACAGATGATTACATCCGCCAGTTTCACAACGTGAACATTAATGTTGCGGTACAGACAGAAAATGGACTCTTTGTACCCGTCATTAGGGATGCGAACAAGAAGGGACTATCCAGCATAGCTGAGGAGGTCAGGCAGCTGGCCCAAAAGGCCAAAGAAAACAGCTTAAAGCCAGAAGATTATGAGGGAGGCACATTTACCGTGACCAATTTGGGAGGTCCATTCGGCATCAAACAATTCTGTGCCATTATTAACCCTCCTCAATCAGGCATTCTTGCGGTTGGATCAGCTGAGAAGAGGGTTGTACCTAGTTCTGGGCCCGAGCTATACAAGTTTGCTTCCTACATGTCTGTAACACTAAGCTGTGATCATCGTGTTATCGATGGTGCAATTGGTGCGGAATGGCTGAAGGCGTTCAAAGGCTACATTGAGAACCCCGAGTCAATGCTGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

546

Amino Acids

58.95

Weight (kDa)

8.25

Isoelectric Point (pI)

55.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	118 - 189	1.7e-18	Biotin-requiring enzyme
E3_binding	PF02817	257 - 292	3.2e-15	e3 binding domain
2-oxoacid_dh	PF00198	314 - 545	1.4e-79	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54220 AT1G54220 AT3G13930
fragaria_vesca	FvH4_6g34140 FvH4_6g34140 FvH4_6g34140
malus_domestica	MD09G1188500.v1.1 MD17G1172600.v1.1
prunus_persica	Prupe.3G045200_v2.0.a1 Prupe.3G045200_v2.0.a1
pyrus_communis	pycom09g10560 pycom17g17050
rosa_chinensis	RchiOBHm_Chr2g0144451
rosa_laevigata	RLG00000020082
rosa_multiflora	Rmu_sc0003475.1_g000006
rosa_roxburghii	Rroxscaffold_2G00101300
rosa_rugosa	Rorug02G0386700 Rorug02G0386800
rosa_samantha	Rh2AG439000 Rh2BG449300 Rh2CG426100 Rh2DG459100
rosa_wichuraiana	Rw2G035920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	289
Acc36I	ACCTGC	2 cut(s)	289, 937
AccB1I	GGYRCC	1 cut(s)	629
AciI	CCGC	6 cut(s)	671, 1108, 1201, 1232, 1460, 1580
AclWI	GGATC	2 cut(s)	551, 1474
AcoI	YGGCCR	1 cut(s)	798
AcsI	RAATTY	1 cut(s)	599
AcuI	CTGAAG	4 cut(s)	141, 149, 431, 1610
AdeI	CACNNNGTG	1 cut(s)	1216
AfaI	GTAC	7 cut(s)	63, 214, 522, 927, 1236, 1260, 1488
AfiI	CCNNNNNNNGG	3 cut(s)	1269, 1448, 1496
AflIII	ACRYGT	2 cut(s)	1040, 1527
AgsI	TTSAA	6 cut(s)	277, 461, 578, 875, 1157, 1600
AjnI	CCWGG	2 cut(s)	221, 436
Alw21I	GWGCWC	1 cut(s)	810
AlwI	GGATC	2 cut(s)	551, 1474
AlwNI	CAGNNNCTG	5 cut(s)	209, 263, 743, 845, 929
Ama87I	CYCGRG	2 cut(s)	1502, 1619
AoxI	GGCC	6 cut(s)	334, 798, 1069, 1323, 1332, 1499
ApaI	GGGCCC	1 cut(s)	1503
ApeKI	GCWGC	7 cut(s)	644, 737, 932, 1139, 1142, 1318, 1630
ApoI	RAATTY	1 cut(s)	599
AseI	ATTAAT	1 cut(s)	1224
Asp700I	GAANNNNTTC	2 cut(s)	364, 1595
AspS9I	GGNCC	6 cut(s)	534, 842, 1324, 1400, 1499, 1500
AsuHPI	GGTGA	4 cut(s)	366, 434, 452, 571
AsuII	TTCGAA	1 cut(s)	32
AvaI	CYCGRG	2 cut(s)	1502, 1619
AvaII	GGWCC	3 cut(s)	534, 842, 1400
BaeGI	GKGCMC	1 cut(s)	1503
BaeI	ACNNNNGTAYC	2 cut(s)	1029, 1062
BanI	GGYRCC	1 cut(s)	629
BanII	GRGCYC	1 cut(s)	1503
BauI	CACGAG	1 cut(s)	891
Bbv12I	GWGCWC	1 cut(s)	810
BbvCI	CCTCAGC	1 cut(s)	1305
BbvI	GCAGC	7 cut(s)	656, 749, 944, 1126, 1129, 1330, 1617
BccI	CCATC	6 cut(s)	478, 494, 524, 625, 842, 1559
BceAI	ACGGC	2 cut(s)	80, 299
BciT130I	CCWGG	2 cut(s)	223, 438
BclI	TGATCA	1 cut(s)	1549
BfaI	CTAG	4 cut(s)	177, 248, 402, 1491
BfmI	CTRYAG	3 cut(s)	474, 642, 930
BfuAI	ACCTGC	2 cut(s)	289, 937
BglI	GCCNNNNNGGC	1 cut(s)	1331
BisI	GCNGC	7 cut(s)	645, 738, 933, 1140, 1143, 1319, 1631
BlsI	GCNGC	7 cut(s)	646, 739, 934, 1141, 1144, 1320, 1632
Bme1390I	CCNGG	2 cut(s)	223, 438
Bme18I	GGWCC	3 cut(s)	534, 842, 1400
BmeT110I	CYCGRG	2 cut(s)	1502, 1619
BmgT120I	GGNCC	6 cut(s)	534, 842, 1324, 1400, 1499, 1500
BmiI	GGNNCC	6 cut(s)	535, 536, 631, 816, 1164, 1501
BmrFI	CCNGG	2 cut(s)	223, 438
BmrI	ACTGGG	2 cut(s)	138, 726
BmsI	GCATC	7 cut(s)	20, 94, 319, 632, 1103, 1264, 1419
BmuI	ACTGGG	2 cut(s)	138, 726
BplI	GAGNNNNNCTC	2 cut(s)	1059, 1091
BpmI	CTGGAG	1 cut(s)	780
Bpu10I	CCTNAGC	2 cut(s)	915, 1305
Bpu14I	TTCGAA	1 cut(s)	32
BpuEI	CTTGAG	1 cut(s)	808
Bsa29I	ATCGAT	1 cut(s)	1563
BsaBI	GATNNNNATC	1 cut(s)	504
BsaJI	CCNNGG	2 cut(s)	222, 538
Bsc4I	CCNNNNNNNGG	3 cut(s)	1269, 1448, 1496
Bse1I	ACTGG	5 cut(s)	144, 633, 698, 732, 1204
Bse8I	GATNNNNATC	1 cut(s)	504
BseBI	CCWGG	2 cut(s)	223, 438
BseCI	ATCGAT	1 cut(s)	1563
BseDI	CCNNGG	2 cut(s)	222, 538
BseGI	GGATG	4 cut(s)	21, 592, 1197, 1279
BseJI	GATNNNNATC	1 cut(s)	504
BseLI	CCNNNNNNNGG	3 cut(s)	1269, 1448, 1496
BseMII	CTCAG	5 cut(s)	378, 651, 977, 1296, 1464
BseNI	ACTGG	5 cut(s)	144, 633, 698, 732, 1204
BseRI	GAGGAG	3 cut(s)	596, 1322, 1431
BseSI	GKGCMC	1 cut(s)	1503
BseXI	GCAGC	7 cut(s)	656, 749, 944, 1126, 1129, 1330, 1617
BsgI	GTGCAG	2 cut(s)	756, 942
BshFI	GGCC	6 cut(s)	336, 800, 1071, 1325, 1334, 1501
BshNI	GGYRCC	1 cut(s)	629
BshVI	ATCGAT	1 cut(s)	1563
BsiHKAI	GWGCWC	1 cut(s)	810
BsiHKCI	CYCGRG	2 cut(s)	1502, 1619
BslFI	GGGAC	2 cut(s)	547, 1302
BslI	CCNNNNNNNGG	3 cut(s)	1269, 1448, 1496
BsmFI	GGGAC	2 cut(s)	547, 1302
BsmI	GAATGC	4 cut(s)	52, 366, 494, 1452
BsnI	GGCC	6 cut(s)	336, 800, 1071, 1325, 1334, 1501
BsoBI	CYCGRG	2 cut(s)	1502, 1619
Bsp119I	TTCGAA	1 cut(s)	32
Bsp120I	GGGCCC	1 cut(s)	1499
Bsp1286I	GDGCHC	2 cut(s)	810, 1503
Bsp143I	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
BspACI	CCGC	6 cut(s)	671, 1108, 1201, 1232, 1460, 1580
BspANI	GGCC	6 cut(s)	336, 800, 1071, 1325, 1334, 1501
BspCNI	CTCAG	5 cut(s)	379, 652, 976, 1297, 1465
BspDI	ATCGAT	1 cut(s)	1563
BspLI	GGNNCC	6 cut(s)	535, 536, 631, 816, 1164, 1501
BspMAI	CTGCAG	2 cut(s)	646, 934
BspMI	ACCTGC	2 cut(s)	289, 937
BspPI	GGATC	2 cut(s)	551, 1474
BspQI	GCTCTTC	2 cut(s)	798, 1158
BspT104I	TTCGAA	1 cut(s)	32
BspT107I	GGYRCC	1 cut(s)	629
BsrI	ACTGG	5 cut(s)	144, 633, 698, 732, 1204
BssECI	CCNNGG	2 cut(s)	222, 538
BssMI	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
BssSI	CACGAG	1 cut(s)	891
BssT1I	CCWWGG	1 cut(s)	538
Bst2BI	CACGAG	1 cut(s)	891
Bst2UI	CCWGG	2 cut(s)	223, 438
Bst4CI	ACNGT	4 cut(s)	478, 574, 1033, 1384
Bst6I	CTCTTC	5 cut(s)	573, 798, 870, 1158, 1472
BstAPI	GCANNNNNTGC	3 cut(s)	629, 929, 1577
BstBI	TTCGAA	1 cut(s)	32
BstC8I	GCNNGC	3 cut(s)	75, 334, 1323
BstDEI	CTNAG	8 cut(s)	58, 387, 660, 915, 963, 1305, 1473, 1541
BstENI	CCTNNNNNAGG	1 cut(s)	1446
BstF5I	GGATG	4 cut(s)	21, 592, 1197, 1279
BstKTI	GATC	6 cut(s)	546, 766, 978, 1126, 1469, 1552
BstMBI	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
BstNI	CCWGG	2 cut(s)	223, 438
BstNSI	RCATGY	2 cut(s)	1044, 1531
BstSCI	CCNGG	2 cut(s)	221, 436
BstSFI	CTRYAG	3 cut(s)	474, 642, 930
BstSLI	GKGCMC	1 cut(s)	1503
BstV1I	GCAGC	7 cut(s)	656, 749, 944, 1126, 1129, 1330, 1617
BstXI	CCANNNNNNTGG	1 cut(s)	356
Bsu15I	ATCGAT	1 cut(s)	1563
BsuRI	GGCC	6 cut(s)	336, 800, 1071, 1325, 1334, 1501
BsuTUI	ATCGAT	1 cut(s)	1563
BtsCI	GGATG	4 cut(s)	21, 592, 1197, 1279
BtsIMutI	CAGTG	4 cut(s)	210, 483, 640, 739
BveI	ACCTGC	2 cut(s)	289, 937
Cac8I	GCNNGC	3 cut(s)	75, 334, 1323
CaiI	CAGNNNCTG	5 cut(s)	209, 263, 743, 845, 929
Cfr13I	GGNCC	6 cut(s)	534, 842, 1324, 1400, 1499, 1500
ClaI	ATCGAT	1 cut(s)	1563
Csp6I	GTAC	7 cut(s)	62, 213, 521, 926, 1235, 1259, 1487
CviAII	CATG	7 cut(s)	101, 291, 493, 1041, 1057, 1182, 1528
CviQI	GTAC	7 cut(s)	62, 213, 521, 926, 1235, 1259, 1487
DdeI	CTNAG	8 cut(s)	58, 387, 660, 915, 963, 1305, 1473, 1541
DpnI	GATC	6 cut(s)	545, 765, 977, 1125, 1468, 1551
DpnII	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
DraI	TTTAAA	1 cut(s)	604
DraIII	CACNNNGTG	1 cut(s)	1216
EaeI	YGGCCR	1 cut(s)	798
Eam1104I	CTCTTC	5 cut(s)	573, 798, 870, 1158, 1472
EarI	CTCTTC	5 cut(s)	573, 798, 870, 1158, 1472
EciI	GGCGGA	2 cut(s)	660, 1190
Eco130I	CCWWGG	1 cut(s)	538
Eco24I	GRGCYC	1 cut(s)	1503
Eco32I	GATATC	4 cut(s)	124, 229, 952, 1113
Eco47I	GGWCC	3 cut(s)	534, 842, 1400
Eco57I	CTGAAG	4 cut(s)	141, 149, 431, 1610
Eco88I	CYCGRG	2 cut(s)	1502, 1619
EcoNI	CCTNNNNNAGG	1 cut(s)	1446
EcoO109I	RGGNCCY	2 cut(s)	534, 842
EcoRII	CCWGG	2 cut(s)	221, 436
EcoRV	GATATC	4 cut(s)	124, 229, 952, 1113
EcoT14I	CCWWGG	1 cut(s)	538
EcoT22I	ATGCAT	1 cut(s)	494
EcoT38I	GRGCYC	1 cut(s)	1503
ErhI	CCWWGG	1 cut(s)	538
FaeI	CATG	7 cut(s)	104, 294, 496, 1044, 1060, 1185, 1531
FaqI	GGGAC	2 cut(s)	547, 1302
FatI	CATG	7 cut(s)	100, 290, 492, 1040, 1056, 1181, 1527
FbaI	TGATCA	1 cut(s)	1549
Fnu4HI	GCNGC	7 cut(s)	645, 738, 933, 1140, 1143, 1319, 1631
FokI	GGATG	4 cut(s)	8, 599, 1184, 1286
FriOI	GRGCYC	1 cut(s)	1503
Fsp4HI	GCNGC	7 cut(s)	645, 738, 933, 1140, 1143, 1319, 1631
FspBI	CTAG	4 cut(s)	177, 248, 402, 1491
GluI	GCNGC	7 cut(s)	645, 738, 933, 1140, 1143, 1319, 1631
GsuI	CTGGAG	1 cut(s)	780
HaeIII	GGCC	6 cut(s)	336, 800, 1071, 1325, 1334, 1501
Hin1II	CATG	7 cut(s)	104, 294, 496, 1044, 1060, 1185, 1531
HinfI	GANTC	6 cut(s)	266, 273, 653, 1053, 1251, 1622
HphI	GGTGA	4 cut(s)	366, 434, 452, 571
Hpy166II	GTNNAC	1 cut(s)	1219
Hpy188I	TCNGA	6 cut(s)	160, 265, 328, 388, 661, 966
Hpy188III	TCNNGA	5 cut(s)	127, 270, 759, 845, 987
Hpy8I	GTNNAC	1 cut(s)	1219
Hpy99I	CGWCG	1 cut(s)	141
HpyAV	CCTTC	9 cut(s)	124, 165, 347, 375, 765, 856, 892, 1279, 1585
HpyCH4III	ACNGT	4 cut(s)	478, 574, 1033, 1384
HpyCH4IV	ACGT	1 cut(s)	1215
HpyF3I	CTNAG	8 cut(s)	58, 387, 660, 915, 963, 1305, 1473, 1541
HpySE526I	ACGT	1 cut(s)	1215
Hsp92II	CATG	7 cut(s)	104, 294, 496, 1044, 1060, 1185, 1531
KflI	GGGWCCC	1 cut(s)	534
Ksp22I	TGATCA	1 cut(s)	1549
Kzo9I	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
LguI	GCTCTTC	2 cut(s)	798, 1158
LmnI	GCTCC	1 cut(s)	745
Lsp1109I	GCAGC	7 cut(s)	656, 749, 944, 1126, 1129, 1330, 1617
LweI	GCATC	7 cut(s)	20, 94, 319, 632, 1103, 1264, 1419
MaeI	CTAG	4 cut(s)	177, 248, 402, 1491
MaeII	ACGT	1 cut(s)	1215
MaeIII	GTNAC	5 cut(s)	39, 422, 700, 1384, 1534
MalI	GATC	6 cut(s)	545, 765, 977, 1125, 1468, 1551
MboI	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
MboII	GAAGA	9 cut(s)	424, 509, 590, 815, 887, 985, 1145, 1370, 1489
MfeI	CAATTG	2 cut(s)	305, 1572
MhlI	GDGCHC	2 cut(s)	810, 1503
MluCI	AATT	9 cut(s)	305, 354, 567, 599, 1011, 1131, 1390, 1418, 1572
MlyI	GAGTC	4 cut(s)	662, 1047, 1245, 1631
MmeI	TCCRAC	3 cut(s)	532, 918, 1444
Mph1103I	ATGCAT	1 cut(s)	494
MroXI	GAANNNNTTC	2 cut(s)	364, 1595
MseI	TTAA	6 cut(s)	603, 1119, 1134, 1224, 1349, 1434
MspA1I	CMGCKG	2 cut(s)	1321, 1472
MspR9I	CCNGG	2 cut(s)	223, 438
MunI	CAATTG	2 cut(s)	305, 1572
Mva1269I	GAATGC	4 cut(s)	52, 366, 494, 1452
MvaI	CCWGG	2 cut(s)	223, 438
NdeII	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
NlaIII	CATG	7 cut(s)	104, 294, 496, 1044, 1060, 1185, 1531
NlaIV	GGNNCC	6 cut(s)	535, 536, 631, 816, 1164, 1501
NmuCI	GTSAC	2 cut(s)	422, 1384
NsiI	ATGCAT	1 cut(s)	494
NspI	RCATGY	2 cut(s)	1044, 1531
NspV	TTCGAA	1 cut(s)	32
PaqCI	CACCTGC	1 cut(s)	289
PciI	ACATGT	2 cut(s)	1040, 1527
PciSI	GCTCTTC	2 cut(s)	798, 1158
PctI	GAATGC	4 cut(s)	52, 366, 494, 1452
PdmI	GAANNNNTTC	2 cut(s)	364, 1595
PfeI	GAWTC	2 cut(s)	266, 273
PkrI	GCNGC	7 cut(s)	646, 739, 934, 1141, 1144, 1320, 1632
PleI	GAGTC	4 cut(s)	661, 1047, 1245, 1630
PpsI	GAGTC	4 cut(s)	661, 1047, 1245, 1630
PpuMI	RGGWCCY	2 cut(s)	534, 842
PscI	ACATGT	2 cut(s)	1040, 1527
PshBI	ATTAAT	1 cut(s)	1224
Psp5II	RGGWCCY	2 cut(s)	534, 842
Psp6I	CCWGG	2 cut(s)	221, 436
PspGI	CCWGG	2 cut(s)	221, 436
PspN4I	GGNNCC	6 cut(s)	535, 536, 631, 816, 1164, 1501
PspOMI	GGGCCC	1 cut(s)	1499
PspPI	GGNCC	6 cut(s)	534, 842, 1324, 1400, 1499, 1500
PspPPI	RGGWCCY	2 cut(s)	534, 842
PstI	CTGCAG	2 cut(s)	646, 934
PstNI	CAGNNNCTG	5 cut(s)	209, 263, 743, 845, 929
PvuII	CAGCTG	2 cut(s)	1321, 1472
RsaI	GTAC	7 cut(s)	63, 214, 522, 927, 1236, 1260, 1488
RsaNI	GTAC	7 cut(s)	62, 213, 521, 926, 1235, 1259, 1487
SapI	GCTCTTC	2 cut(s)	798, 1158
SaqAI	TTAA	6 cut(s)	603, 1119, 1134, 1224, 1349, 1434
SatI	GCNGC	7 cut(s)	645, 738, 933, 1140, 1143, 1319, 1631
Sau3AI	GATC	6 cut(s)	543, 763, 975, 1123, 1466, 1549
Sau96I	GGNCC	6 cut(s)	534, 842, 1324, 1400, 1499, 1500
SchI	GAGTC	4 cut(s)	662, 1047, 1245, 1631
ScrFI	CCNGG	2 cut(s)	223, 438
SduI	GDGCHC	2 cut(s)	810, 1503
SfaNI	GCATC	7 cut(s)	20, 94, 319, 632, 1103, 1264, 1419
SfcI	CTRYAG	3 cut(s)	474, 642, 930
SfiI	GGCCNNNNNGGCC	1 cut(s)	1331
SfuI	TTCGAA	1 cut(s)	32
SinI	GGWCC	3 cut(s)	534, 842, 1400
SmlI	CTYRAG	1 cut(s)	823
SmoI	CTYRAG	1 cut(s)	823
Sse9I	AATT	9 cut(s)	305, 354, 567, 599, 1011, 1131, 1390, 1418, 1572
SsiI	CCGC	6 cut(s)	671, 1108, 1201, 1232, 1460, 1580
SspI	AATATT	1 cut(s)	397
SspMI	CTAG	4 cut(s)	177, 248, 402, 1491
StyD4I	CCNGG	2 cut(s)	221, 436
StyI	CCWWGG	1 cut(s)	538
TaaI	ACNGT	4 cut(s)	478, 574, 1033, 1384
TaiI	ACGT	1 cut(s)	1218
TaqI	TCGA	3 cut(s)	32, 986, 1563
TasI	AATT	9 cut(s)	305, 354, 567, 599, 1011, 1131, 1390, 1418, 1572
TfiI	GAWTC	2 cut(s)	266, 273
Tru1I	TTAA	6 cut(s)	603, 1119, 1134, 1224, 1349, 1434
Tru9I	TTAA	6 cut(s)	603, 1119, 1134, 1224, 1349, 1434
TscAI	CASTG	4 cut(s)	210, 483, 640, 739
TseFI	GTSAC	2 cut(s)	422, 1384
TseI	GCWGC	7 cut(s)	644, 737, 932, 1139, 1142, 1318, 1630
Tsp45I	GTSAC	2 cut(s)	422, 1384
TspDTI	ATGAA	3 cut(s)	279, 312, 1170
TspGWI	ACGGA	1 cut(s)	985
TspRI	CASTG	4 cut(s)	210, 483, 640, 739
VpaK11BI	GGWCC	3 cut(s)	534, 842, 1400
VspI	ATTAAT	1 cut(s)	1224
XagI	CCTNNNNNAGG	1 cut(s)	1446
XapI	RAATTY	1 cut(s)	599
XceI	RCATGY	2 cut(s)	1044, 1531
XcmI	CCANNNNNNNNNTGG	1 cut(s)	682
XmnI	GAANNNNTTC	2 cut(s)	364, 1595
XspI	CTAG	4 cut(s)	177, 248, 402, 1491
Zsp2I	ATGCAT	1 cut(s)	494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.