AT1G54650

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007209
Physical Location & Seq
Reverse (-)
20405467 .. 20407753
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54650.2

Sequence Viewer

Length: 900 bp
ATGGAGAAAAGAGAAGAAGCAGGAGAGTATCATTCCAAAGATTTCGAATGGGAATTTCTCAAGAACATCGTCGAGAACGATCCTTCTCTCTCCCACCATCTCCACCACCACCACCAAAATCTCTCCAAATATTTACCAGATTCGCAGCCATGGCAAGATTTTCACTCCCGTCACTCCTCCGGAAAATTCTTCAAGGAAAGAAGGTATCTGTTGAAGGAATTTCCTGAATTAGTTTCTTGTGGAGAGAACTCAAAACTTTTGGAAATTGGTTGTGGTAATGGCAGTACCGTGCTTCCGATTTTACGTGGAAGCAAGAACATTACTGTGTATGCTTGTGATTGTAGCAGTGACGCTCTTGTTAGGACTAAAGAGAACATTGATCGCGCTATTTCTTCGGTTGATAACTTTCATTCTTTTTGTTGTGACTTTTCGACTTCTGAGTTTCCAGATTGGGTGGCATGTGATCGTTGTCGAGACAAGTTTATGCTTAACCACTCAGGAGGGAGTGAGAGCAAACATTGCATCGGTGGGGTGGATTTTGTCACTCTGATATTTACATTGTCGGCAGTGCCTAAAGAAAGAATGCCGAGAGCTATCAAGGAATGCTTTGCTGTGCTGAAACCTGGTGGTCTGCTCTTATTTAGAGATTATGGATTATATGACATGACTATGCTACGGTTTGAGCCAGAGAAACGTGTAGGATTTAGGGAATATGTGCGGTCTGATGGAACCCTTTCCTATTTCTTTTGTCTCGACACTGCCAGGAAACTATTTACTGATGCTGGATTCATTGAGGTTGAGCTTGAATATTGTTGTGTCAAAGCAGTAAACCGGAGAAAGGGTAAGGACATGTACCGAGTTTGGGTTCATGGGAAGTTCCAAAAGCCCTTCTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_23

Protein Analysis

299

Amino Acids

34.63

Weight (kDa)

7.17

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_12 PF08242 107 - 164 5.4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 384
AccIII TCCGGA 1 cut(s) 179
AciI CCGC 1 cut(s) 718
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 3 cut(s) 53, 185, 218
AfaI GTAC 2 cut(s) 286, 854
AfiI CCNNNNNNNGG 2 cut(s) 838, 862
AflIII ACRYGT 2 cut(s) 694, 849
AgsI TTSAA 3 cut(s) 193, 214, 806
AjnI CCWGG 2 cut(s) 622, 761
AluBI AGCT 2 cut(s) 593, 802
AluI AGCT 2 cut(s) 593, 802
Alw26I GTCTC 2 cut(s) 468, 755
AlwI GGATC 1 cut(s) 74
Aor13HI TCCGGA 1 cut(s) 179
ApeKI GCWGC 1 cut(s) 145
ApoI RAATTY 3 cut(s) 53, 185, 218
Asp700I GAANNNNTTC 1 cut(s) 733
AspLEI GCGC 1 cut(s) 386
AsuII TTCGAA 1 cut(s) 45
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 105, 719
BciT130I CCWGG 2 cut(s) 624, 763
BcoDI GTCTC 2 cut(s) 468, 755
BisI GCNGC 1 cut(s) 146
BlsI GCNGC 1 cut(s) 147
Bme1390I CCNGG 2 cut(s) 624, 763
BmiI GGNNCC 1 cut(s) 730
BmrFI CCNGG 2 cut(s) 624, 763
BmsI GCATC 2 cut(s) 531, 769
Bpu14I TTCGAA 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 44
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 149
BsaWI WCCGGW 2 cut(s) 179, 831
BsaXI ACNNNNNCTCC 2 cut(s) 826, 856
Bsc4I CCNNNNNNNGG 2 cut(s) 838, 862
Bse3DI GCAATG 1 cut(s) 517
BseAI TCCGGA 1 cut(s) 179
BseBI CCWGG 2 cut(s) 624, 763
BseDI CCNNGG 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 838, 862
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 2 cut(s) 429, 510
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 384
BsiSI CCGG 2 cut(s) 180, 832
BslI CCNNNNNNNGG 2 cut(s) 838, 862
BsmAI GTCTC 2 cut(s) 468, 755
BsmI GAATGC 2 cut(s) 588, 608
Bsp119I TTCGAA 1 cut(s) 45
Bsp13I TCCGGA 1 cut(s) 179
Bsp143I GATC 3 cut(s) 79, 379, 463
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 1 cut(s) 718
BspCNI CTCAG 2 cut(s) 430, 509
BspEI TCCGGA 1 cut(s) 179
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 730
BspPI GGATC 1 cut(s) 74
BspT104I TTCGAA 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 517
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 3 cut(s) 79, 379, 463
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 2 cut(s) 624, 763
Bst4CI ACNGT 3 cut(s) 289, 325, 678
BstAPI GCANNNNNTGC 1 cut(s) 519
BstBAI YACGTR 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 45
BstDEI CTNAG 2 cut(s) 438, 496
BstDSI CCRYGG 1 cut(s) 149
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 3 cut(s) 82, 382, 466
BstMAI GTCTC 2 cut(s) 468, 755
BstMBI GATC 3 cut(s) 79, 379, 463
BstMWI GCNNNNNNNGC 2 cut(s) 151, 519
BstNI CCWGG 2 cut(s) 624, 763
BstNSI RCATGY 2 cut(s) 462, 853
BstSCI CCNGG 2 cut(s) 622, 761
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 1 cut(s) 157
BtgI CCRYGG 1 cut(s) 149
BtsI GCAGTG 3 cut(s) 352, 573, 756
BtsIMutI CAGTG 3 cut(s) 352, 573, 756
CfoI GCGC 1 cut(s) 386
CseI GACGC 1 cut(s) 359
CsiI ACCWGGT 1 cut(s) 622
Csp6I GTAC 2 cut(s) 285, 853
CviAII CATG 5 cut(s) 150, 459, 664, 850, 869
CviJI RGCY 5 cut(s) 148, 593, 685, 802, 886
CviKI_1 RGCY 5 cut(s) 148, 593, 685, 802, 886
CviQI GTAC 2 cut(s) 285, 853
DdeI CTNAG 2 cut(s) 438, 496
DpnI GATC 3 cut(s) 81, 381, 465
DpnII GATC 3 cut(s) 79, 379, 463
Eco130I CCWWGG 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 622, 761
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 5 cut(s) 153, 462, 667, 853, 872
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FatI CATG 5 cut(s) 149, 458, 663, 849, 868
Fnu4HI GCNGC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 146
GlaI GCGC 1 cut(s) 385
GluI GCNGC 1 cut(s) 146
HapII CCGG 2 cut(s) 180, 832
HgaI GACGC 1 cut(s) 359
HhaI GCGC 1 cut(s) 386
Hin1II CATG 5 cut(s) 153, 462, 667, 853, 872
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HinfI GANTC 2 cut(s) 140, 786
HpaII CCGG 2 cut(s) 180, 832
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 4 cut(s) 297, 439, 549, 724
Hpy188III TCNNGA 8 cut(s) 61, 73, 180, 224, 446, 473, 498, 752
Hpy8I GTNNAC 1 cut(s) 829
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 4 cut(s) 93, 195, 208, 898
HpyCH4III ACNGT 3 cut(s) 289, 325, 678
HpyCH4IV ACGT 2 cut(s) 304, 694
HpyCH4V TGCA 1 cut(s) 522
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 519
HpyF3I CTNAG 2 cut(s) 438, 496
HpySE526I ACGT 2 cut(s) 304, 694
Hsp92II CATG 5 cut(s) 153, 462, 667, 853, 872
HspAI GCGC 1 cut(s) 384
Kpn2I TCCGGA 1 cut(s) 179
Kzo9I GATC 3 cut(s) 79, 379, 463
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 2 cut(s) 531, 769
MabI ACCWGGT 1 cut(s) 622
MaeII ACGT 2 cut(s) 304, 694
MaeIII GTNAC 4 cut(s) 170, 347, 422, 541
MalI GATC 3 cut(s) 81, 381, 465
MboI GATC 3 cut(s) 79, 379, 463
MboII GAAGA 3 cut(s) 26, 181, 384
MluCI AATT 6 cut(s) 53, 185, 218, 227, 264, 895
MnlI CCTC 3 cut(s) 187, 494, 787
MroI TCCGGA 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 733
MseI TTAA 2 cut(s) 489, 898
MslI CAYNNNNRTG 2 cut(s) 323, 668
MspI CCGG 2 cut(s) 180, 832
MspR9I CCNGG 2 cut(s) 624, 763
Mva1269I GAATGC 2 cut(s) 588, 608
MvaI CCWGG 2 cut(s) 624, 763
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 151, 519
NcoI CCATGG 1 cut(s) 149
NdeII GATC 3 cut(s) 79, 379, 463
NlaIII CATG 5 cut(s) 153, 462, 667, 853, 872
NlaIV GGNNCC 1 cut(s) 730
NmeAIII GCCGAG 1 cut(s) 612
NmuCI GTSAC 4 cut(s) 170, 347, 422, 541
NspI RCATGY 2 cut(s) 462, 853
NspV TTCGAA 1 cut(s) 45
PciI ACATGT 1 cut(s) 849
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 2 cut(s) 588, 608
PdmI GAANNNNTTC 1 cut(s) 733
PfeI GAWTC 2 cut(s) 140, 786
PkrI GCNGC 1 cut(s) 147
Ppu21I YACGTR 1 cut(s) 305
PscI ACATGT 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 622, 761
PspGI CCWGG 2 cut(s) 622, 761
PspN4I GGNNCC 1 cut(s) 730
RsaI GTAC 2 cut(s) 286, 854
RsaNI GTAC 2 cut(s) 285, 853
RseI CAYNNNNRTG 2 cut(s) 323, 668
SaqAI TTAA 2 cut(s) 489, 898
SatI GCNGC 1 cut(s) 146
Sau3AI GATC 3 cut(s) 79, 379, 463
ScrFI CCNGG 2 cut(s) 624, 763
SetI ASST 7 cut(s) 206, 307, 595, 625, 697, 798, 804
SexAI ACCWGGT 1 cut(s) 622
SfaNI GCATC 2 cut(s) 531, 769
SfuI TTCGAA 1 cut(s) 45
SmiMI CAYNNNNRTG 2 cut(s) 323, 668
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 6 cut(s) 53, 185, 218, 227, 264, 895
SsiI CCGC 1 cut(s) 718
SspI AATATT 2 cut(s) 131, 809
StyD4I CCNGG 2 cut(s) 622, 761
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 3 cut(s) 289, 325, 678
TaiI ACGT 2 cut(s) 307, 697
TaqI TCGA 5 cut(s) 45, 72, 431, 472, 753
TasI AATT 6 cut(s) 53, 185, 218, 227, 264, 895
TfiI GAWTC 2 cut(s) 140, 786
Tru1I TTAA 2 cut(s) 489, 898
Tru9I TTAA 2 cut(s) 489, 898
TscAI CASTG 3 cut(s) 352, 573, 763
TseFI GTSAC 4 cut(s) 170, 347, 422, 541
TseI GCWGC 1 cut(s) 145
Tsp45I GTSAC 4 cut(s) 170, 347, 422, 541
TspDTI ATGAA 3 cut(s) 398, 778, 857
TspRI CASTG 3 cut(s) 352, 573, 763
XapI RAATTY 3 cut(s) 53, 185, 218
XceI RCATGY 2 cut(s) 462, 853
XmnI GAANNNNTTC 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.