Rmu_co8435467.1_g000001

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8435467.1
OG0007209
Physical Location & Seq
Forward (+)
1 .. 1418
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8435467.1_g000001.1.cds

Sequence Viewer

Length: 754 bp
gagcgacgccgtttcgcgccaaaccctaaccactaccggcgcaaaagcgcgggctctgtgttctcaaatcggtgaagacgagaagatgaagaaagcagaatacttcagcaaggactttgactgggacgagctgagagtcgaggtggaaaacgacccgtcgtttagctaccatttgctcccaatccaaccttctctttcagttgcagaacaaggtgaatctcaaccgtggaatgactttcatcaccgccattcatctgggaagttcttcaaggaaaggcgatatttgttgcaagaattccctgaactagttagctgtgaggagaattctagggttttggaggtggggtgtggcaatggcagcactgttcttccaatgttgaggggcaacgagaaagtcacggtttatgcatgtgattggagcactgaggcccttgagagggctaaggagacgatttatgcttctaacattgtttctatcgagcatcgtttccatacgttttgttgtgatttctctacctctgggtttccaacatggttgacctacaatccttgtcaagcaaattttgaacaaacttcagatggtagagggaatagtgaaacctgtcttaatgattcaagttcattgaaagaaagcagatgttgcattggtggggttgattttgttaccttgtctccttgtcctttgaaacttgtagcctctgatggtttggcatttcatagttcaactttaccatattcagttgataaattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_23

Protein Analysis

250

Amino Acids

28.06

Weight (kDa)

5.05

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 17, 50
AciI CCGC 2 cut(s) 50, 245
AcsI RAATTY 4 cut(s) 294, 323, 560, 747
AcuI CTGAAG 2 cut(s) 89, 559
AcyI GRCGYC 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 436, 437
AgsI TTSAA 6 cut(s) 269, 567, 616, 626, 686, 724
AhlI ACTAGT 1 cut(s) 305
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 3 cut(s) 131, 166, 313
AluI AGCT 3 cut(s) 131, 166, 313
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 2 cut(s) 441, 676
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 1 cut(s) 358
ApoI RAATTY 4 cut(s) 294, 323, 560, 747
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 3 cut(s) 19, 42, 50
AspS9I GGNCC 1 cut(s) 428
AsuHPI GGTGA 3 cut(s) 84, 225, 234
BanII GRGCYC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 1 cut(s) 370
BccI CCATC 2 cut(s) 573, 696
BcoDI GTCTC 2 cut(s) 441, 676
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 2 cut(s) 306, 328
BisI GCNGC 1 cut(s) 359
BlsI GCNGC 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 428
BmrI ACTGGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 491
BmuI ACTGGG 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 82
Bpu10I CCTNAGC 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 7
BsaJI CCNNGG 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 656, 686
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 437
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 436, 437
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 2 cut(s) 123, 415
BseNI ACTGG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 333
BseXI GCAGC 1 cut(s) 370
Bsh1236I CGCG 2 cut(s) 17, 50
BshFI GGCC 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 423
BsiSI CCGG 1 cut(s) 37
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 2 cut(s) 436, 437
BsmAI GTCTC 2 cut(s) 441, 676
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 56, 423
BspACI CCGC 2 cut(s) 50, 245
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 2 cut(s) 124, 416
BspFNI CGCG 2 cut(s) 17, 50
BsrDI GCAATG 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 7
Bst4CI ACNGT 3 cut(s) 226, 365, 401
BstACI GRCGYC 1 cut(s) 7
BstAPI GCANNNNNTGC 1 cut(s) 640
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 3 cut(s) 132, 424, 442
BstDSI CCRYGG 1 cut(s) 225
BstFNI CGCG 2 cut(s) 17, 50
BstHHI GCGC 3 cut(s) 19, 42, 50
BstMAI GTCTC 2 cut(s) 441, 676
BstMWI GCNNNNNNNGC 2 cut(s) 358, 640
BstNSI RCATGY 1 cut(s) 412
BstUI CGCG 2 cut(s) 17, 50
BstV1I GCAGC 1 cut(s) 370
BstV2I GAAGAC 1 cut(s) 82
BstXI CCANNNNNNTGG 1 cut(s) 255
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 361, 421
Cac8I GCNNGC 1 cut(s) 52
CfoI GCGC 3 cut(s) 19, 42, 50
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 1 cut(s) 428
CseI GACGC 1 cut(s) 15
CviAII CATG 2 cut(s) 409, 532
CviJI RGCY 7 cut(s) 54, 131, 166, 313, 429, 441, 696
CviKI_1 RGCY 7 cut(s) 54, 131, 166, 313, 429, 441, 696
DdeI CTNAG 3 cut(s) 132, 424, 442
Eco24I GRGCYC 1 cut(s) 56
Eco57I CTGAAG 2 cut(s) 89, 559
EcoO109I RGGNCCY 1 cut(s) 428
EcoRI GAATTC 2 cut(s) 294, 323
EcoT22I ATGCAT 1 cut(s) 410
EcoT38I GRGCYC 1 cut(s) 56
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 2 cut(s) 412, 535
FaiI YATR 7 cut(s) 406, 410, 457, 493, 533, 718, 734
FaqI GGGAC 1 cut(s) 138
FatI CATG 2 cut(s) 408, 531
FauI CCCGC 1 cut(s) 43
Fnu4HI GCNGC 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 1 cut(s) 359
FspBI CTAG 2 cut(s) 306, 328
GlaI GCGC 3 cut(s) 18, 41, 49
GluI GCNGC 1 cut(s) 359
HaeIII GGCC 1 cut(s) 429
HapII CCGG 1 cut(s) 37
HgaI GACGC 1 cut(s) 15
HhaI GCGC 3 cut(s) 19, 42, 50
Hin1I GRCGYC 1 cut(s) 7
Hin1II CATG 2 cut(s) 412, 535
Hin6I GCGC 3 cut(s) 17, 40, 48
HinP1I GCGC 3 cut(s) 17, 40, 48
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 3 cut(s) 136, 216, 612
HpaII CCGG 1 cut(s) 37
HphI GGTGA 3 cut(s) 84, 225, 234
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 2 cut(s) 578, 701
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 2 cut(s) 9, 161
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 3 cut(s) 226, 365, 401
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 4 cut(s) 204, 290, 408, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 358, 640
HpyF3I CTNAG 3 cut(s) 132, 424, 442
HpySE526I ACGT 1 cut(s) 495
Hsp92I GRCGYC 1 cut(s) 7
Hsp92II CATG 2 cut(s) 412, 535
HspAI GCGC 3 cut(s) 17, 40, 48
LmnI GCTCC 2 cut(s) 181, 418
LpnPI CCDG 6 cut(s) 50, 107, 241, 313, 505, 614
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 1 cut(s) 491
MaeI CTAG 2 cut(s) 306, 328
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 2 cut(s) 395, 662
MboII GAAGA 5 cut(s) 87, 95, 101, 257, 360
MhlI GDGCHC 2 cut(s) 56, 423
MluCI AATT 4 cut(s) 294, 323, 560, 747
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 2 cut(s) 209, 552
MnlI CCTC 9 cut(s) 134, 311, 332, 373, 419, 430, 527, 579, 707
Mph1103I ATGCAT 1 cut(s) 410
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 1 cut(s) 607
MspI CCGG 1 cut(s) 37
MvnI CGCG 2 cut(s) 17, 50
MwoI GCNNNNNNNGC 2 cut(s) 358, 640
NlaIII CATG 2 cut(s) 412, 535
NmuCI GTSAC 1 cut(s) 395
NsiI ATGCAT 1 cut(s) 410
NspI RCATGY 1 cut(s) 412
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 216, 612
PkrI GCNGC 1 cut(s) 360
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 428
SaqAI TTAA 1 cut(s) 607
SatI GCNGC 1 cut(s) 359
Sau96I GGNCC 1 cut(s) 428
SchI GAGTC 1 cut(s) 145
SduI GDGCHC 2 cut(s) 56, 423
SfaNI GCATC 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 432
SmoI CTYRAG 1 cut(s) 432
SpeI ACTAGT 1 cut(s) 305
Sse9I AATT 4 cut(s) 294, 323, 560, 747
SsiI CCGC 2 cut(s) 50, 245
SspMI CTAG 2 cut(s) 306, 328
TaaI ACNGT 3 cut(s) 226, 365, 401
TaiI ACGT 1 cut(s) 498
TaqI TCGA 2 cut(s) 139, 478
TasI AATT 4 cut(s) 294, 323, 560, 747
TfiI GAWTC 2 cut(s) 216, 612
Tru1I TTAA 1 cut(s) 607
Tru9I TTAA 1 cut(s) 607
TscAI CASTG 2 cut(s) 368, 428
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 5 cut(s) 102, 228, 241, 610, 705
TspRI CASTG 2 cut(s) 368, 428
XapI RAATTY 4 cut(s) 294, 323, 560, 747
XceI RCATGY 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 2 cut(s) 306, 328
Zsp2I ATGCAT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.