Rroxscaffold_6G00427870

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007209
Physical Location & Seq
Reverse (-)
48419385 .. 48427262
7878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427870.1

Sequence Viewer

Length: 1026 bp
ATGAGGGAGAGAAAGCGACGCCGTTTCGCGCCAACCCTAACCTCTACCGGCTCAAAAGCGCGGGCTCTGTGTTCTCAAATCGGTGAAGACGAGAAGATGAAGAAAGCAGAATACTTCAGCAAGGACTTTGACTGGGACGAGCTGAGAGTCGAGGTGGAAAACGACCCGTCGTTTAGCTACCATTTGCTCCCATTCCAACCTTCTTTTTCAGTTGCAGAACAAGCTGAATCTCAACCGTGGAATGACTTTCATCACCGCCATTCATCTGGGAAGTTCTTCAAGGAAAGGCGATATTTGTTGCAAGAATTCCCTGAACTAGTTAGCTGTGAGGAGAATTCTAGGGTTTTGGAGGTGGGGTGTGGCAATGGCAGCACTGTTCTTCCAATATTGAGGGGCAACGAGAAAGTCACGGTTTATGCATGTGATTGTAGCACTGAGGCCCTTGAGAGGGTTAAGGAGACGATTTATGCTTCTAACATTGTTTCTATTGAGCATCGTTTCCATACATTTTGTTGTGATTTCTCTACCTCTGGGTTTCCAACATGGTTGACCTACAATCCTTGTCAAGCAAATTTTGAACAAACTTCAGATGGTAGAGGGAATAGTGAAACCTGTCTTAATGATTCAAGTTCATTGAAAGAAAGCAGATGTTGCATTGGTGGGGTTGATTTTGTTACCTTGATATTCACACTGTCGGCTTTATCACTTCAGAGGATGCCAAAGTCCATCAAGGAGTGTTTTTCTGTTATGAGACCTGGAGGCCTGCTTCTATTTAGGGATTATGGCCTTTATGACATGAGCATGCTTCGGTTTGAGATGGACAAAAGAGTGGGATTCAGGGAGTATATGCGATCAGATGGAACACATTCTTATTTCTTCTCATTGGATACTGTAAGGGATCTATTTATGGGAGCAGGCTTTATTGAGCTTGAGCTTGAATACTGCTGTGTTAAGTCGCTGAATCGTCGAAATGGGAAGAGCATGCGAAGGGTGTGGGTTCACGGAAAGTTCCAAAAGCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_23

Protein Analysis

341

Amino Acids

39.41

Weight (kDa)

7.05

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 94 - 307 1.1e-15 Methyltransferase domain
Methyltransf_25 PF13649 115 - 166 5.9e-06 Methyltransferase domain
Methyltransf_12 PF08242 116 - 256 1.3e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 29, 61
AciI CCGC 2 cut(s) 61, 256
AclWI GGATC 1 cut(s) 906
AcsI RAATTY 3 cut(s) 305, 334, 571
AcuI CTGAAG 3 cut(s) 100, 570, 692
AcyI GRCGYC 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 447, 448
AgsI TTSAA 5 cut(s) 280, 578, 627, 637, 938
AhlI ACTAGT 1 cut(s) 316
AjnI CCWGG 1 cut(s) 754
AluBI AGCT 6 cut(s) 142, 177, 224, 324, 928, 934
AluI AGCT 6 cut(s) 142, 177, 224, 324, 928, 934
Alw26I GTCTC 2 cut(s) 452, 745
AlwI GGATC 1 cut(s) 906
AoxI GGCC 3 cut(s) 438, 760, 784
ApeKI GCWGC 1 cut(s) 369
ApoI RAATTY 3 cut(s) 305, 334, 571
Asp700I GAANNNNTTC 2 cut(s) 275, 865
AspLEI GCGC 2 cut(s) 31, 61
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 95, 245
BanII GRGCYC 1 cut(s) 67
BbsI GAAGAC 1 cut(s) 93
BbvI GCAGC 1 cut(s) 381
BccI CCATC 4 cut(s) 584, 734, 811, 851
BceAI ACGGC 1 cut(s) 6
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 1 cut(s) 880
BcoDI GTCTC 2 cut(s) 452, 745
BcuI ACTAGT 1 cut(s) 316
BfaI CTAG 2 cut(s) 317, 339
BfuI GTATCC 1 cut(s) 880
BisI GCNGC 1 cut(s) 370
BlsI GCNGC 1 cut(s) 371
Bme1390I CCNGG 1 cut(s) 756
BmgT120I GGNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 756
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 502, 705
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 777
BpuEI CTTGAG 2 cut(s) 464, 950
BsaHI GRCGYC 1 cut(s) 19
BsaI GGTCTC 1 cut(s) 745
BsaJI CCNNGG 1 cut(s) 236
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 447, 448
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 370
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 1 cut(s) 720
BseLI CCNNNNNNNGG 2 cut(s) 447, 448
BseMI GCAATG 1 cut(s) 370
BseMII CTCAG 2 cut(s) 134, 426
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 1 cut(s) 381
Bsh1236I CGCG 2 cut(s) 29, 61
BshFI GGCC 3 cut(s) 440, 762, 786
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 447, 448
BsmAI GTCTC 2 cut(s) 452, 745
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 3 cut(s) 440, 762, 786
Bso31I GGTCTC 1 cut(s) 745
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 2 cut(s) 851, 898
BspACI CCGC 2 cut(s) 61, 256
BspANI GGCC 3 cut(s) 440, 762, 786
BspCNI CTCAG 2 cut(s) 135, 427
BspFNI CGCG 2 cut(s) 29, 61
BspPI GGATC 1 cut(s) 906
BspQI GCTCTTC 1 cut(s) 971
BspTNI GGTCTC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 370
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 2 cut(s) 851, 898
BssNI GRCGYC 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 237, 376, 412, 693, 892
Bst6I CTCTTC 1 cut(s) 971
BstACI GRCGYC 1 cut(s) 19
BstAPI GCANNNNNTGC 1 cut(s) 651
BstC8I GCNNGC 5 cut(s) 63, 764, 803, 916, 983
BstDEI CTNAG 2 cut(s) 143, 435
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 720
BstFNI CGCG 2 cut(s) 29, 61
BstHHI GCGC 2 cut(s) 31, 61
BstKTI GATC 2 cut(s) 854, 901
BstMAI GTCTC 2 cut(s) 452, 745
BstMBI GATC 2 cut(s) 851, 898
BstMWI GCNNNNNNNGC 3 cut(s) 221, 369, 651
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 3 cut(s) 423, 805, 985
BstSCI CCNGG 1 cut(s) 754
BstUI CGCG 2 cut(s) 29, 61
BstV1I GCAGC 1 cut(s) 381
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 898
BstXI CCANNNNNNTGG 1 cut(s) 266
BstYI RGATCY 1 cut(s) 898
BsuI GTATCC 1 cut(s) 880
BsuRI GGCC 3 cut(s) 440, 762, 786
BtgI CCRYGG 1 cut(s) 236
BtsCI GGATG 1 cut(s) 720
BtsIMutI CAGTG 3 cut(s) 372, 432, 689
Cac8I GCNNGC 5 cut(s) 63, 764, 803, 916, 983
CfoI GCGC 2 cut(s) 31, 61
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 1 cut(s) 27
CviAII CATG 5 cut(s) 420, 543, 796, 802, 982
DdeI CTNAG 2 cut(s) 143, 435
DpnI GATC 2 cut(s) 853, 900
DpnII GATC 2 cut(s) 851, 898
Eam1104I CTCTTC 1 cut(s) 971
EarI CTCTTC 1 cut(s) 971
Eco147I AGGCCT 1 cut(s) 762
Eco24I GRGCYC 1 cut(s) 67
Eco31I GGTCTC 1 cut(s) 745
Eco57I CTGAAG 3 cut(s) 100, 570, 692
EcoO109I RGGNCCY 1 cut(s) 439
EcoRI GAATTC 2 cut(s) 305, 334
EcoRII CCWGG 1 cut(s) 754
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 452
FaeI CATG 5 cut(s) 423, 546, 799, 805, 985
FaqI GGGAC 1 cut(s) 149
FatI CATG 5 cut(s) 419, 542, 795, 801, 981
FauI CCCGC 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 370
FokI GGATG 1 cut(s) 727
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 370
FspBI CTAG 2 cut(s) 317, 339
GlaI GCGC 2 cut(s) 30, 60
GluI GCNGC 1 cut(s) 370
GsuI CTGGAG 1 cut(s) 777
HaeIII GGCC 3 cut(s) 440, 762, 786
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 27
HhaI GCGC 2 cut(s) 31, 61
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 5 cut(s) 423, 546, 799, 805, 985
Hin6I GCGC 2 cut(s) 29, 59
HinP1I GCGC 2 cut(s) 29, 59
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 5 cut(s) 147, 227, 623, 834, 961
HpaII CCGG 1 cut(s) 48
HphI GGTGA 2 cut(s) 95, 245
Hpy166II GTNNAC 2 cut(s) 549, 1000
Hpy188I TCNGA 3 cut(s) 589, 711, 856
Hpy8I GTNNAC 2 cut(s) 549, 1000
Hpy99I CGWCG 3 cut(s) 21, 172, 969
HpyAV CCTTC 2 cut(s) 210, 981
HpyCH4III ACNGT 5 cut(s) 237, 376, 412, 693, 892
HpyCH4V TGCA 4 cut(s) 215, 301, 419, 654
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 369, 651
HpyF3I CTNAG 2 cut(s) 143, 435
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 5 cut(s) 423, 546, 799, 805, 985
HspAI GCGC 2 cut(s) 29, 59
Kzo9I GATC 2 cut(s) 851, 898
LguI GCTCTTC 1 cut(s) 971
LmnI GCTCC 2 cut(s) 192, 911
Lsp1109I GCAGC 1 cut(s) 381
LweI GCATC 2 cut(s) 502, 705
MaeI CTAG 2 cut(s) 317, 339
MaeIII GTNAC 2 cut(s) 406, 673
MalI GATC 2 cut(s) 853, 900
MboI GATC 2 cut(s) 851, 898
MboII GAAGA 7 cut(s) 98, 106, 112, 268, 371, 868, 988
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 3 cut(s) 305, 334, 571
MlyI GAGTC 1 cut(s) 156
MmeI TCCRAC 2 cut(s) 220, 563
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 2 cut(s) 275, 865
MseI TTAA 3 cut(s) 453, 618, 951
MslI CAYNNNNRTG 1 cut(s) 800
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 756
MvaI CCWGG 1 cut(s) 756
MvnI CGCG 2 cut(s) 29, 61
MwoI GCNNNNNNNGC 3 cut(s) 221, 369, 651
NdeII GATC 2 cut(s) 851, 898
NlaIII CATG 5 cut(s) 423, 546, 799, 805, 985
NmuCI GTSAC 1 cut(s) 406
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 3 cut(s) 423, 805, 985
PaeI GCATGC 2 cut(s) 805, 985
PceI AGGCCT 1 cut(s) 762
PciSI GCTCTTC 1 cut(s) 971
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 2 cut(s) 275, 865
PfeI GAWTC 4 cut(s) 227, 623, 834, 961
PkrI GCNGC 1 cut(s) 371
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspPI GGNCC 1 cut(s) 439
PsuI RGATCY 1 cut(s) 898
RseI CAYNNNNRTG 1 cut(s) 800
SapI GCTCTTC 1 cut(s) 971
SaqAI TTAA 3 cut(s) 453, 618, 951
SatI GCNGC 1 cut(s) 370
Sau3AI GATC 2 cut(s) 851, 898
Sau96I GGNCC 1 cut(s) 439
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 1 cut(s) 756
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 2 cut(s) 502, 705
SmiMI CAYNNNNRTG 1 cut(s) 800
SmlI CTYRAG 2 cut(s) 443, 929
SmoI CTYRAG 2 cut(s) 443, 929
SpeI ACTAGT 1 cut(s) 316
SphI GCATGC 2 cut(s) 805, 985
Sse9I AATT 3 cut(s) 305, 334, 571
SseBI AGGCCT 1 cut(s) 762
SsiI CCGC 2 cut(s) 61, 256
SspI AATATT 1 cut(s) 387
SspMI CTAG 2 cut(s) 317, 339
StuI AGGCCT 1 cut(s) 762
StyD4I CCNGG 1 cut(s) 754
TaaI ACNGT 5 cut(s) 237, 376, 412, 693, 892
TaqI TCGA 2 cut(s) 150, 967
TasI AATT 3 cut(s) 305, 334, 571
TfiI GAWTC 4 cut(s) 227, 623, 834, 961
Tru1I TTAA 3 cut(s) 453, 618, 951
Tru9I TTAA 3 cut(s) 453, 618, 951
TscAI CASTG 3 cut(s) 379, 439, 696
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 369
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 4 cut(s) 113, 239, 252, 621
TspGWI ACGGA 1 cut(s) 1017
TspRI CASTG 3 cut(s) 379, 439, 696
XapI RAATTY 3 cut(s) 305, 334, 571
XceI RCATGY 3 cut(s) 423, 805, 985
XmnI GAANNNNTTC 2 cut(s) 275, 865
XspI CTAG 2 cut(s) 317, 339
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.