pycom04g20390

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007209
Physical Location & Seq
Reverse (-)
21914271 .. 21917175
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20390.1

Sequence Viewer

Length: 1146 bp
ATGCGATTAAATTTCACTTTGTATTTATTTACAAATAGTAATATAATTGCGAACTGTTTGTTTATTTTTTTTAACAATTTATGTTTCAAACAGCTGCTGTTGGAAGTCCTCCTTCTCCAACCCCGGACATCGCTCGCTATCAGCAGAATTACAGTCTCTCCGTTTCCAATGAGAAAAGCAGAGTACTTCAGCAAGGACTTCGAGTGGGAAGACCTCCGAGCTCAGGTGGAAAACGACCCGTCGTTCAGCTACCACTTGCTGCCTTTCGAACCCACCTCCTTCTCACTGAATTCTGTCGCAACAGATCAATCCCATTCCTCCGCTGCCGACGGCTCTCACGCGTGGAACAGATTTCACCACCGTCATTCCTCCGGCAAATTCTTCAAGGAAAGAAGATATTTGTTAGAAGAATTCCCTGAACTAGTTAATTGGAAAGAGAATTCTAAGGTTTTGGAGGTGGGATGCGGTAATGGCAGCACCATTCTTCCAATATTACGTGGCAATGAGAACATCATTGTTTATGCTTGTGATTGTAGCACTGAGGCCCTTGACAGGGTTAAGGAGACTATAGATGCCTCTAACATTGCAGCTGTTGAACATCGCTTCCATCCATTTTGTCATGATTTTTCTGTAACTGCGTTTCCAACATGGTTGGCCTGCAATCCTTGTCGAGAAAACTTCACACAAACATCGCAGCAGCACTTGACAGATGGTAGAGGCGAAAGTGAAACGGATCCAAATTATAAATATGCATCGAAAGAAAGTAAATGTTGTGTTGGTGGGGTTGATTTTGTTACCTTGATATTCACACTGTCAGCATTACCACTTAAAAGGATGCCAGCGTCCATCAAGGAGTGTTTTTCTCTTTTAAAACCCGGAGGCCTGCTGTTCTTTAGGGATTATGGCCTCTATGACATGACCATGCTTCGATTTGAGGAGGACAAAAGAGTGCAATTCAGGGAGTATATGCGATCAGATGGAACCCGTTCTTATTTCTTCTGTCTAGATACTGTGAGGGATCTATTTGTGCGTGCCGGCTTCAAACAGCTTGAGCTCGAATACTGCTGTGTTAAGTCAGTGAATCGTCGGAAGGGTAAGAGCATGCTAAGGGTGTGGGTTCATGGAAAGTTCCAGAAGCCGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_23

Protein Analysis

382

Amino Acids

44.13

Weight (kDa)

8.14

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 133 - 350 8.1e-14 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AccII CGCG 1 cut(s) 341
AciI CCGC 2 cut(s) 321, 465
AclWI GGATC 3 cut(s) 728, 741, 1026
AcsI RAATTY 5 cut(s) 10, 289, 377, 410, 439
AcuI CTGAAG 1 cut(s) 172
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 4 cut(s) 223, 552, 553, 1138
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 4 cut(s) 88, 385, 596, 1042
AhlI ACTAGT 1 cut(s) 421
AloI GAACNNNNNNTCC 2 cut(s) 588, 620
AluBI AGCT 6 cut(s) 94, 221, 249, 590, 1048, 1054
AluI AGCT 6 cut(s) 94, 221, 249, 590, 1048, 1054
Alw21I GWGCWC 2 cut(s) 223, 1056
Alw26I GTCTC 2 cut(s) 160, 557
AlwI GGATC 3 cut(s) 728, 741, 1026
AlwNI CAGNNNCTG 1 cut(s) 97
AoxI GGCC 4 cut(s) 543, 654, 880, 904
ApeKI GCWGC 7 cut(s) 94, 259, 323, 474, 587, 694, 697
ApoI RAATTY 5 cut(s) 10, 289, 377, 410, 439
Asp700I GAANNNNTTC 1 cut(s) 985
AspS9I GGNCC 1 cut(s) 544
AsuC2I CCSGG 2 cut(s) 124, 876
AsuHPI GGTGA 1 cut(s) 347
AsuII TTCGAA 1 cut(s) 267
BamHI GGATCC 1 cut(s) 733
BanII GRGCYC 2 cut(s) 223, 1056
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 2 cut(s) 223, 1056
BbvI GCAGC 7 cut(s) 81, 246, 310, 486, 599, 706, 709
BccI CCATC 4 cut(s) 615, 704, 854, 971
BceAI ACGGC 1 cut(s) 346
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BcnI CCSGG 2 cut(s) 124, 876
BcoDI GTCTC 2 cut(s) 160, 557
BcuI ACTAGT 1 cut(s) 421
BfaI CTAG 2 cut(s) 422, 1004
BfmI CTRYAG 1 cut(s) 567
BisI GCNGC 7 cut(s) 95, 260, 324, 475, 588, 695, 698
BlsI GCNGC 7 cut(s) 96, 261, 325, 476, 589, 696, 699
BmcAI AGTACT 1 cut(s) 185
Bme1390I CCNGG 2 cut(s) 124, 876
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 2 cut(s) 735, 982
BmrFI CCNGG 2 cut(s) 124, 876
BmsI GCATC 4 cut(s) 452, 562, 761, 825
BpiI GAAGAC 1 cut(s) 216
Bpu10I CCTNAGC 2 cut(s) 222, 1106
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 1070
BpuMI CCSGG 2 cut(s) 124, 876
BsaAI YACGTR 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 552, 553, 1138
Bse118I RCCGGY 2 cut(s) 1034, 1138
Bse3DI GCAATG 2 cut(s) 508, 582
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 3 cut(s) 467, 607, 840
BseLI CCNNNNNNNGG 4 cut(s) 223, 552, 553, 1138
BseMI GCAATG 2 cut(s) 508, 582
BseMII CTCAG 2 cut(s) 236, 531
BseRI GAGGAG 1 cut(s) 950
BseXI GCAGC 7 cut(s) 81, 246, 310, 486, 599, 706, 709
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 4 cut(s) 545, 656, 882, 906
BsiHKAI GWGCWC 2 cut(s) 223, 1056
BsiSI CCGG 5 cut(s) 124, 372, 876, 1035, 1139
BslI CCNNNNNNNGG 4 cut(s) 223, 552, 553, 1138
BsmAI GTCTC 2 cut(s) 160, 557
BsnI GGCC 4 cut(s) 545, 656, 882, 906
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 2 cut(s) 223, 1056
Bsp143I GATC 4 cut(s) 304, 733, 971, 1018
BspACI CCGC 2 cut(s) 321, 465
BspANI GGCC 4 cut(s) 545, 656, 882, 906
BspCNI CTCAG 2 cut(s) 235, 532
BspFNI CGCG 1 cut(s) 341
BspHI TCATGA 1 cut(s) 619
BspLI GGNNCC 2 cut(s) 735, 982
BspPI GGATC 3 cut(s) 728, 741, 1026
BspT104I TTCGAA 1 cut(s) 267
BsrDI GCAATG 2 cut(s) 508, 582
BsrFI RCCGGY 2 cut(s) 1034, 1138
BssAI RCCGGY 2 cut(s) 1034, 1138
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 4 cut(s) 304, 733, 971, 1018
Bst4CI ACNGT 5 cut(s) 56, 154, 362, 813, 1012
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 7 cut(s) 135, 658, 840, 884, 1032, 1036, 1103
BstDEI CTNAG 4 cut(s) 222, 444, 540, 1106
BstF5I GGATG 3 cut(s) 467, 607, 840
BstFNI CGCG 1 cut(s) 341
BstKTI GATC 4 cut(s) 307, 736, 974, 1021
BstMAI GTCTC 2 cut(s) 160, 557
BstMBI GATC 4 cut(s) 304, 733, 971, 1018
BstMWI GCNNNNNNNGC 1 cut(s) 471
BstNSI RCATGY 1 cut(s) 1105
BstSCI CCNGG 2 cut(s) 122, 874
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 7 cut(s) 81, 246, 310, 486, 599, 706, 709
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 2 cut(s) 733, 1018
BstYI RGATCY 2 cut(s) 733, 1018
BsuRI GGCC 4 cut(s) 545, 656, 882, 906
BtgZI GCGATG 3 cut(s) 114, 584, 675
BtsCI GGATG 3 cut(s) 467, 607, 840
BtsIMutI CAGTG 4 cut(s) 284, 537, 809, 1083
Cac8I GCNNGC 7 cut(s) 135, 658, 840, 884, 1032, 1036, 1103
CaiI CAGNNNCTG 1 cut(s) 97
CciI TCATGA 1 cut(s) 619
Cfr10I RCCGGY 2 cut(s) 1034, 1138
Cfr13I GGNCC 1 cut(s) 544
CseI GACGC 1 cut(s) 831
Csp6I GTAC 1 cut(s) 184
CviAII CATG 6 cut(s) 620, 648, 916, 922, 1102, 1121
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 4 cut(s) 222, 444, 540, 1106
DpnI GATC 4 cut(s) 306, 735, 973, 1020
DpnII GATC 4 cut(s) 304, 733, 971, 1018
DraI TTTAAA 1 cut(s) 870
Ecl136II GAGCTC 2 cut(s) 221, 1054
Eco147I AGGCCT 1 cut(s) 882
Eco24I GRGCYC 2 cut(s) 223, 1056
Eco53kI GAGCTC 2 cut(s) 221, 1054
Eco57I CTGAAG 1 cut(s) 172
EcoICRI GAGCTC 2 cut(s) 221, 1054
EcoO109I RGGNCCY 1 cut(s) 544
EcoRI GAATTC 3 cut(s) 289, 410, 439
EcoT22I ATGCAT 1 cut(s) 754
EcoT38I GRGCYC 2 cut(s) 223, 1056
FaeI CATG 6 cut(s) 623, 651, 919, 925, 1105, 1124
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FatI CATG 6 cut(s) 619, 647, 915, 921, 1101, 1120
Fnu4HI GCNGC 7 cut(s) 95, 260, 324, 475, 588, 695, 698
FokI GGATG 3 cut(s) 474, 594, 847
FriOI GRGCYC 2 cut(s) 223, 1056
Fsp4HI GCNGC 7 cut(s) 95, 260, 324, 475, 588, 695, 698
FspBI CTAG 2 cut(s) 422, 1004
GluI GCNGC 7 cut(s) 95, 260, 324, 475, 588, 695, 698
HaeIII GGCC 4 cut(s) 545, 656, 882, 906
HapII CCGG 5 cut(s) 124, 372, 876, 1035, 1139
HgaI GACGC 1 cut(s) 831
Hin1II CATG 6 cut(s) 623, 651, 919, 925, 1105, 1124
HinfI GANTC 1 cut(s) 1081
HpaII CCGG 5 cut(s) 124, 372, 876, 1035, 1139
HphI GGTGA 1 cut(s) 347
Hpy188I TCNGA 3 cut(s) 218, 976, 1089
Hpy188III TCNNGA 4 cut(s) 620, 671, 1004, 1132
Hpy99I CGWCG 3 cut(s) 244, 332, 1089
HpyAV CCTTC 3 cut(s) 122, 289, 1084
HpyCH4III ACNGT 5 cut(s) 56, 154, 362, 813, 1012
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 4 cut(s) 587, 660, 752, 952
HpyF10VI GCNNNNNNNGC 1 cut(s) 471
HpyF3I CTNAG 4 cut(s) 222, 444, 540, 1106
HpySE526I ACGT 1 cut(s) 496
Hsp92II CATG 6 cut(s) 623, 651, 919, 925, 1105, 1124
KroI GCCGGC 1 cut(s) 1034
KroNI GCCGGC 1 cut(s) 1036
Kzo9I GATC 4 cut(s) 304, 733, 971, 1018
Lsp1109I GCAGC 7 cut(s) 81, 246, 310, 486, 599, 706, 709
LweI GCATC 4 cut(s) 452, 562, 761, 825
MaeI CTAG 2 cut(s) 422, 1004
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 2 cut(s) 631, 793
MalI GATC 4 cut(s) 306, 735, 973, 1020
MboI GATC 4 cut(s) 304, 733, 971, 1018
MboII GAAGA 6 cut(s) 221, 373, 405, 419, 476, 988
MflI RGATCY 2 cut(s) 733, 1018
MhlI GDGCHC 2 cut(s) 223, 1056
MluI ACGCGT 1 cut(s) 339
MmeI TCCRAC 4 cut(s) 81, 142, 668, 1067
Mph1103I ATGCAT 1 cut(s) 754
MroNI GCCGGC 1 cut(s) 1034
MroXI GAANNNNTTC 1 cut(s) 985
MseI TTAA 7 cut(s) 8, 72, 426, 558, 828, 869, 1071
MslI CAYNNNNRTG 1 cut(s) 920
MspA1I CMGCKG 3 cut(s) 94, 323, 590
MspI CCGG 5 cut(s) 124, 372, 876, 1035, 1139
MspR9I CCNGG 2 cut(s) 124, 876
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 1 cut(s) 471
NaeI GCCGGC 1 cut(s) 1036
NciI CCSGG 2 cut(s) 124, 876
NdeII GATC 4 cut(s) 304, 733, 971, 1018
NgoMIV GCCGGC 1 cut(s) 1034
NlaIII CATG 6 cut(s) 623, 651, 919, 925, 1105, 1124
NlaIV GGNNCC 2 cut(s) 735, 982
NsiI ATGCAT 1 cut(s) 754
NspI RCATGY 1 cut(s) 1105
NspV TTCGAA 1 cut(s) 267
PaeI GCATGC 1 cut(s) 1105
PagI TCATGA 1 cut(s) 619
PceI AGGCCT 1 cut(s) 882
PdiI GCCGGC 1 cut(s) 1036
PdmI GAANNNNTTC 1 cut(s) 985
PfeI GAWTC 1 cut(s) 1081
PkrI GCNGC 7 cut(s) 96, 261, 325, 476, 589, 696, 699
Ppu21I YACGTR 1 cut(s) 497
PsiI TTATAA 1 cut(s) 744
Psp124BI GAGCTC 2 cut(s) 223, 1056
PspN4I GGNNCC 2 cut(s) 735, 982
PspPI GGNCC 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 97
PsuI RGATCY 2 cut(s) 733, 1018
PvuII CAGCTG 2 cut(s) 94, 590
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 920
SacI GAGCTC 2 cut(s) 223, 1056
SaqAI TTAA 7 cut(s) 8, 72, 426, 558, 828, 869, 1071
SatI GCNGC 7 cut(s) 95, 260, 324, 475, 588, 695, 698
Sau3AI GATC 4 cut(s) 304, 733, 971, 1018
Sau96I GGNCC 1 cut(s) 544
ScaI AGTACT 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 124, 876
SduI GDGCHC 2 cut(s) 223, 1056
SfaNI GCATC 4 cut(s) 452, 562, 761, 825
SfcI CTRYAG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
SpeI ACTAGT 1 cut(s) 421
SphI GCATGC 1 cut(s) 1105
SseBI AGGCCT 1 cut(s) 882
SsiI CCGC 2 cut(s) 321, 465
SspI AATATT 1 cut(s) 492
SspMI CTAG 2 cut(s) 422, 1004
SstI GAGCTC 2 cut(s) 223, 1056
StuI AGGCCT 1 cut(s) 882
StyD4I CCNGG 2 cut(s) 122, 874
TaaI ACNGT 5 cut(s) 56, 154, 362, 813, 1012
TaiI ACGT 1 cut(s) 499
TaqI TCGA 6 cut(s) 201, 267, 670, 755, 928, 1056
TatI WGTACW 1 cut(s) 183
TfiI GAWTC 1 cut(s) 1081
Tru1I TTAA 7 cut(s) 8, 72, 426, 558, 828, 869, 1071
Tru9I TTAA 7 cut(s) 8, 72, 426, 558, 828, 869, 1071
TscAI CASTG 4 cut(s) 291, 544, 816, 1083
TseI GCWGC 7 cut(s) 94, 259, 323, 474, 587, 694, 697
TspDTI ATGAA 1 cut(s) 1109
TspGWI ACGGA 2 cut(s) 150, 746
TspRI CASTG 4 cut(s) 291, 544, 816, 1083
XapI RAATTY 5 cut(s) 10, 289, 377, 410, 439
XbaI TCTAGA 1 cut(s) 1003
XceI RCATGY 1 cut(s) 1105
XmnI GAANNNNTTC 1 cut(s) 985
XspI CTAG 2 cut(s) 422, 1004
ZrmI AGTACT 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.