FvH4_6g02330

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007209
Physical Location & Seq
Reverse (-)
1350205 .. 1353505
3301 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02330.t1

Sequence Viewer

Length: 732 bp
ATGAAGAAAGCAGAGTACTTCAGCAAGGACTTTGAGTGGGACGAGCTGAGAGTTGAGGTGGAAAACGACCCGTCGTTGAGCTACCACTTGGTCCCATTCGAGCCCTCTCTTTCAGTTGCAGAAGAAGAAGAAGCTGAATCTCAGCCGTGGAATGACTTTCATCACCGCCATGCATCTGGGAAGTTCTTCAAGGAAAGGCGATATTTGTTGCAAGAATTCCCTGAACTAGTTGGGTGTGAGGAGAATTCTAAGGTTTTGGAGGTGGGGTGTGGTAATGGCAGCACTGTTCTTCCAATATTGAGGGGCAATGAGAAAGTCACTGTTTACGCATGTGATTGTAGCACTGAGGCCCTTGAGAGAGTTAAGGAGACAATATATGCTTCCAACATTGTTTCTATTGAGCATCGCTTCCATACATTTTGTTGTGATTTTTCTACCTCTGGGTTTCCATCATGGTTGGCCTGCAATCCTTGTCAAGCAAATTTTGAACAAACTTCAGATGGTAGAGGGAAAAACGAAAGCTATCTTACTGATTCAAGTTCATTGAAAGAAAGCAGATGTTGCATTGGTGGGGTTGATTTTGTTACCTTGATATTCACATTGTCGGCATTATCACTTCAAAGGATGCCAAAGTCCATCAATGAGTGTTTTTCTGTTATGAAACCAGGAGGCCTGCTTCTATTTAGAGATTATGAAGTTCGGTGTGATGAAATTCAGGCCTTTATGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_23

Protein Analysis

244

Amino Acids

27.66

Weight (kDa)

4.86

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 65 - 242 2.5e-11 Methyltransferase domain
Methyltransf_25 PF13649 85 - 137 2.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 166
AcsI RAATTY 4 cut(s) 215, 244, 481, 711
AcuI CTGAAG 2 cut(s) 4, 480
AfaI GTAC 1 cut(s) 17
AgsI TTSAA 5 cut(s) 190, 488, 537, 547, 620
AhlI ACTAGT 1 cut(s) 226
AjnI CCWGG 1 cut(s) 664
AluBI AGCT 4 cut(s) 46, 81, 134, 522
AluI AGCT 4 cut(s) 46, 81, 134, 522
Alw26I GTCTC 1 cut(s) 362
AoxI GGCC 4 cut(s) 348, 459, 670, 717
ApeKI GCWGC 1 cut(s) 279
ApoI RAATTY 4 cut(s) 215, 244, 481, 711
Asp700I GAANNNNTTC 1 cut(s) 185
AspS9I GGNCC 2 cut(s) 91, 349
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 291
BccI CCATC 3 cut(s) 457, 494, 644
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 1 cut(s) 666
BcoDI GTCTC 1 cut(s) 362
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 1 cut(s) 227
BisI GCNGC 1 cut(s) 280
BlsI GCNGC 1 cut(s) 281
BmcAI AGTACT 1 cut(s) 17
Bme1390I CCNGG 1 cut(s) 666
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 91, 349
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 666
BmsI GCATC 3 cut(s) 182, 412, 615
BpuEI CTTGAG 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 146
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 3 cut(s) 38, 155, 336
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 291
BshFI GGCC 4 cut(s) 350, 461, 672, 719
BslFI GGGAC 2 cut(s) 53, 77
BsmAI GTCTC 1 cut(s) 362
BsmFI GGGAC 2 cut(s) 53, 77
BsnI GGCC 4 cut(s) 350, 461, 672, 719
Bsp1286I GDGCHC 1 cut(s) 105
BspACI CCGC 1 cut(s) 166
BspANI GGCC 4 cut(s) 350, 461, 672, 719
BspCNI CTCAG 3 cut(s) 39, 154, 337
BspLI GGNNCC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 2 cut(s) 286, 322
BstAPI GCANNNNNTGC 1 cut(s) 561
BstC8I GCNNGC 2 cut(s) 463, 674
BstDEI CTNAG 4 cut(s) 47, 141, 249, 345
BstDSI CCRYGG 1 cut(s) 146
BstF5I GGATG 1 cut(s) 630
BstMAI GTCTC 1 cut(s) 362
BstMWI GCNNNNNNNGC 1 cut(s) 561
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 664
BstV1I GCAGC 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 176
BsuRI GGCC 4 cut(s) 350, 461, 672, 719
BtgI CCRYGG 1 cut(s) 146
BtgZI GCGATG 1 cut(s) 389
BtsCI GGATG 1 cut(s) 630
BtsIMutI CAGTG 3 cut(s) 282, 318, 342
Cac8I GCNNGC 2 cut(s) 463, 674
Cfr13I GGNCC 2 cut(s) 91, 349
Csp6I GTAC 1 cut(s) 16
CviAII CATG 4 cut(s) 170, 330, 453, 729
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 4 cut(s) 47, 141, 249, 345
Eco147I AGGCCT 2 cut(s) 672, 719
Eco24I GRGCYC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 91
Eco57I CTGAAG 2 cut(s) 4, 480
EcoO109I RGGNCCY 1 cut(s) 349
EcoRI GAATTC 2 cut(s) 215, 244
EcoRII CCWGG 1 cut(s) 664
EcoT22I ATGCAT 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 4 cut(s) 173, 333, 456, 732
FaqI GGGAC 2 cut(s) 53, 77
FatI CATG 4 cut(s) 169, 329, 452, 728
Fnu4HI GCNGC 1 cut(s) 280
FokI GGATG 1 cut(s) 637
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 280
FspBI CTAG 1 cut(s) 227
GluI GCNGC 1 cut(s) 280
HaeIII GGCC 4 cut(s) 350, 461, 672, 719
Hin1II CATG 4 cut(s) 173, 333, 456, 732
HinfI GANTC 2 cut(s) 137, 533
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 1 cut(s) 499
Hpy8I GTNNAC 1 cut(s) 325
Hpy99I CGWCG 1 cut(s) 76
HpyCH4III ACNGT 2 cut(s) 286, 322
HpyCH4V TGCA 5 cut(s) 119, 173, 211, 465, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 561
HpyF3I CTNAG 4 cut(s) 47, 141, 249, 345
Hsp92II CATG 4 cut(s) 173, 333, 456, 732
LpnPI CCDG 8 cut(s) 162, 234, 426, 475, 651, 678, 686, 701
Lsp1109I GCAGC 1 cut(s) 291
LweI GCATC 3 cut(s) 182, 412, 615
MaeI CTAG 1 cut(s) 227
MaeIII GTNAC 2 cut(s) 316, 583
MboII GAAGA 6 cut(s) 16, 134, 137, 140, 178, 281
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 4 cut(s) 215, 244, 481, 711
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 9 cut(s) 49, 115, 232, 253, 294, 340, 448, 500, 662
Mph1103I ATGCAT 1 cut(s) 175
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 666
MwoI GCNNNNNNNGC 1 cut(s) 561
NlaIII CATG 4 cut(s) 173, 333, 456, 732
NlaIV GGNNCC 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 316
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 1 cut(s) 333
PceI AGGCCT 2 cut(s) 672, 719
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 2 cut(s) 137, 533
PkrI GCNGC 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 2 cut(s) 91, 349
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 1 cut(s) 280
Sau96I GGNCC 2 cut(s) 91, 349
ScaI AGTACT 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 1 cut(s) 105
SetI ASST 9 cut(s) 48, 60, 83, 136, 255, 264, 440, 524, 590
SfaNI GCATC 3 cut(s) 182, 412, 615
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SpeI ACTAGT 1 cut(s) 226
Sse9I AATT 4 cut(s) 215, 244, 481, 711
SseBI AGGCCT 2 cut(s) 672, 719
SsiI CCGC 1 cut(s) 166
SspI AATATT 1 cut(s) 297
SspMI CTAG 1 cut(s) 227
StuI AGGCCT 2 cut(s) 672, 719
StyD4I CCNGG 1 cut(s) 664
TaaI ACNGT 2 cut(s) 286, 322
TaqI TCGA 1 cut(s) 99
TasI AATT 4 cut(s) 215, 244, 481, 711
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 2 cut(s) 137, 533
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 3 cut(s) 289, 325, 349
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 1 cut(s) 279
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 6 cut(s) 17, 149, 531, 674, 708, 723
TspRI CASTG 3 cut(s) 289, 325, 349
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 4 cut(s) 215, 244, 481, 711
XceI RCATGY 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 1 cut(s) 227
ZrmI AGTACT 1 cut(s) 17
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.