MD04G1230000.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007209
Physical Location & Seq
Reverse (-)
31018458 .. 31021143
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1230000.v1.1.491

Sequence Viewer

Length: 978 bp
ATGAGAAAAGCAGAGTACTTCAGCAAGGACTTCGAGTGGGAAGACCTCCGAGCTCAGGTGGAAAACGACCCGTCGTTCAGCTACCACTTGCGGCCTTTCGAACCTACCTCCTTCTCACTGAATTCTGTCGCAACAGATCAATCCCGTTCCTCCGCCGCCGACGGCTCTCACGCGTGGAACAGTTTTCACCACCGTCATTCCTCCGGCAAATTCTTCAAGGAAAGAAGATATTTGTTAGAAGAATTCCCTGAACTAGTTAATTGGAAAGGAAATTCTAAGGTTTTGGAGGTGGGATGCGGTAATGGCAGCACCGTTCTTCCAATATTATGTGGCAATGAGAACATCGTTGTTTATGCTTGTGATTGTAGCACTGAGGCCCTTGACAGGGTTAAGGAGACTATAGATGCCTCTAACATTGCAGCTGTTGAACATCATTTCCATCCATTTTGTTGTGATTTTTCTGTAACTACGTTTCCAACGTGGTTGGCCTGCAATCCTTGTCGAGAAAACTTCACACAAACATCGCAGCAGCGCTTGACAGATGGTAGAGGCGAAAGTGAAACGGATCCAAATTATACATATGCATTGAAAGAAAGTAAATGTTGTGTTGGTGGGGTTGATTTTGTTACCTTGATATTCACACTGTCAGCATTACCTCTTAAAAGGATGCCAGAGTCCATCAAGGAGTGTTTTTCTGTTTTAAAACCCGGAGGCCTGCTGTTCTTTAGGGATTATGGCCTCTATGACATGACCATGCTTCGATTTGAGGAGGACAAAAGAGTGCAATTCAGGGAGTATATGCGATCAGATGGAACCCGTTCTTATTTCTTCTGTCTAGATACTGTGAGGGATCTATTTGTGGGTGCCGGCTTCAAACAGCTTGAGCTCGAATACTGCTGTGTTAAGTCAGTGAACCGTCGGAAGGGTAAGAGCATGCAAAGGGTGTGGGTTCATGGAAAGTTCCAGAAGCCGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_12 Methyltransf_23

Protein Analysis

326

Amino Acids

37.41

Weight (kDa)

6.52

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 82 - 294 1.4e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 863
AccII CGCG 1 cut(s) 173
AciI CCGC 4 cut(s) 91, 153, 156, 297
AclWI GGATC 3 cut(s) 560, 573, 858
AcsI RAATTY 4 cut(s) 121, 209, 242, 271
AcuI CTGAAG 1 cut(s) 4
AfaI GTAC 1 cut(s) 17
AfeI AGCGCT 1 cut(s) 533
AfiI CCNNNNNNNGG 5 cut(s) 55, 384, 385, 922, 970
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 4 cut(s) 217, 428, 589, 874
AhlI ACTAGT 1 cut(s) 253
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
AluBI AGCT 5 cut(s) 53, 81, 422, 880, 886
AluI AGCT 5 cut(s) 53, 81, 422, 880, 886
Alw21I GWGCWC 2 cut(s) 55, 888
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 3 cut(s) 560, 573, 858
Aor51HI AGCGCT 1 cut(s) 533
AoxI GGCC 5 cut(s) 92, 375, 486, 712, 736
ApeKI GCWGC 4 cut(s) 306, 419, 526, 529
ApoI RAATTY 4 cut(s) 121, 209, 242, 271
Asp700I GAANNNNTTC 1 cut(s) 817
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 1 cut(s) 376
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 1 cut(s) 179
AsuII TTCGAA 1 cut(s) 99
BamHI GGATCC 1 cut(s) 565
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 2 cut(s) 55, 888
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 55, 888
BbvI GCAGC 4 cut(s) 318, 431, 538, 541
BccI CCATC 4 cut(s) 447, 536, 686, 803
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BcnI CCSGG 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 389
BcuI ACTAGT 1 cut(s) 253
BfaI CTAG 2 cut(s) 254, 836
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 535
BisI GCNGC 6 cut(s) 92, 156, 307, 420, 527, 530
BlsI GCNGC 6 cut(s) 93, 157, 308, 421, 528, 531
BmcAI AGTACT 1 cut(s) 17
Bme1390I CCNGG 1 cut(s) 708
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 3 cut(s) 567, 814, 865
BmrFI CCNGG 1 cut(s) 708
BmsI GCATC 3 cut(s) 284, 394, 657
BpiI GAAGAC 1 cut(s) 48
Bpu10I CCTNAGC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 708
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 384, 385, 922, 970
Bse118I RCCGGY 2 cut(s) 866, 970
Bse3DI GCAATG 2 cut(s) 340, 414
BseGI GGATG 3 cut(s) 299, 439, 672
BseLI CCNNNNNNNGG 5 cut(s) 55, 384, 385, 922, 970
BseMI GCAATG 2 cut(s) 340, 414
BseMII CTCAG 2 cut(s) 68, 363
BseRI GAGGAG 1 cut(s) 782
BseXI GCAGC 4 cut(s) 318, 431, 538, 541
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 5 cut(s) 94, 377, 488, 714, 738
BshNI GGYRCC 1 cut(s) 863
BsiHKAI GWGCWC 2 cut(s) 55, 888
BsiSI CCGG 4 cut(s) 204, 708, 867, 971
BslI CCNNNNNNNGG 5 cut(s) 55, 384, 385, 922, 970
BsmAI GTCTC 1 cut(s) 389
BsnI GGCC 5 cut(s) 94, 377, 488, 714, 738
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 2 cut(s) 55, 888
Bsp143I GATC 4 cut(s) 136, 565, 803, 850
BspACI CCGC 4 cut(s) 91, 153, 156, 297
BspANI GGCC 5 cut(s) 94, 377, 488, 714, 738
BspCNI CTCAG 2 cut(s) 67, 364
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 3 cut(s) 567, 814, 865
BspPI GGATC 3 cut(s) 560, 573, 858
BspT104I TTCGAA 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 863
BsrDI GCAATG 2 cut(s) 340, 414
BsrFI RCCGGY 2 cut(s) 866, 970
BssAI RCCGGY 2 cut(s) 866, 970
BssMI GATC 4 cut(s) 136, 565, 803, 850
Bst4CI ACNGT 6 cut(s) 182, 194, 313, 645, 844, 917
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 4 cut(s) 490, 716, 868, 935
BstDEI CTNAG 3 cut(s) 54, 276, 372
BstF5I GGATG 3 cut(s) 299, 439, 672
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 4 cut(s) 139, 568, 806, 853
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 4 cut(s) 136, 565, 803, 850
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstNSI RCATGY 1 cut(s) 937
BstSCI CCNGG 1 cut(s) 706
BstSFI CTRYAG 1 cut(s) 399
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 4 cut(s) 318, 431, 538, 541
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 2 cut(s) 565, 850
BstYI RGATCY 2 cut(s) 565, 850
BsuRI GGCC 5 cut(s) 94, 377, 488, 714, 738
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 3 cut(s) 299, 439, 672
BtsIMutI CAGTG 4 cut(s) 116, 369, 641, 915
Cac8I GCNNGC 4 cut(s) 490, 716, 868, 935
CfoI GCGC 1 cut(s) 534
Cfr10I RCCGGY 2 cut(s) 866, 970
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 1 cut(s) 16
CspCI CAANNNNNGTGG 2 cut(s) 926, 961
CviAII CATG 4 cut(s) 748, 754, 934, 953
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 3 cut(s) 54, 276, 372
DpnI GATC 4 cut(s) 138, 567, 805, 852
DpnII GATC 4 cut(s) 136, 565, 803, 850
DraI TTTAAA 1 cut(s) 702
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 2 cut(s) 53, 886
Eco147I AGGCCT 1 cut(s) 714
Eco24I GRGCYC 2 cut(s) 55, 888
Eco47III AGCGCT 1 cut(s) 533
Eco53kI GAGCTC 2 cut(s) 53, 886
Eco57I CTGAAG 1 cut(s) 4
EcoICRI GAGCTC 2 cut(s) 53, 886
EcoO109I RGGNCCY 1 cut(s) 376
EcoRI GAATTC 2 cut(s) 121, 242
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 2 cut(s) 55, 888
FaeI CATG 4 cut(s) 751, 757, 937, 956
FatI CATG 4 cut(s) 747, 753, 933, 952
FauNDI CATATG 1 cut(s) 580
Fnu4HI GCNGC 6 cut(s) 92, 156, 307, 420, 527, 530
FokI GGATG 3 cut(s) 306, 426, 679
FriOI GRGCYC 2 cut(s) 55, 888
Fsp4HI GCNGC 6 cut(s) 92, 156, 307, 420, 527, 530
FspBI CTAG 2 cut(s) 254, 836
GlaI GCGC 1 cut(s) 533
GluI GCNGC 6 cut(s) 92, 156, 307, 420, 527, 530
HaeII RGCGCY 1 cut(s) 535
HaeIII GGCC 5 cut(s) 94, 377, 488, 714, 738
HapII CCGG 4 cut(s) 204, 708, 867, 971
HhaI GCGC 1 cut(s) 534
Hin1II CATG 4 cut(s) 751, 757, 937, 956
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HinfI GANTC 1 cut(s) 674
HpaII CCGG 4 cut(s) 204, 708, 867, 971
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 1 cut(s) 913
Hpy188I TCNGA 3 cut(s) 50, 808, 921
Hpy188III TCNNGA 3 cut(s) 503, 836, 964
Hpy8I GTNNAC 1 cut(s) 913
Hpy99I CGWCG 3 cut(s) 76, 164, 921
HpyAV CCTTC 2 cut(s) 121, 916
HpyCH4III ACNGT 6 cut(s) 182, 194, 313, 645, 844, 917
HpyCH4IV ACGT 2 cut(s) 470, 479
HpyCH4V TGCA 5 cut(s) 419, 492, 584, 784, 937
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 3 cut(s) 54, 276, 372
HpySE526I ACGT 2 cut(s) 470, 479
Hsp92II CATG 4 cut(s) 751, 757, 937, 956
HspAI GCGC 1 cut(s) 532
KroI GCCGGC 1 cut(s) 866
KroNI GCCGGC 1 cut(s) 868
Kzo9I GATC 4 cut(s) 136, 565, 803, 850
Lsp1109I GCAGC 4 cut(s) 318, 431, 538, 541
LweI GCATC 3 cut(s) 284, 394, 657
MaeI CTAG 2 cut(s) 254, 836
MaeII ACGT 2 cut(s) 470, 479
MaeIII GTNAC 2 cut(s) 463, 625
MalI GATC 4 cut(s) 138, 567, 805, 852
MboI GATC 4 cut(s) 136, 565, 803, 850
MboII GAAGA 6 cut(s) 53, 205, 237, 251, 308, 820
MflI RGATCY 2 cut(s) 565, 850
MhlI GDGCHC 2 cut(s) 55, 888
MluCI AATT 7 cut(s) 121, 209, 242, 259, 271, 571, 785
MluI ACGCGT 1 cut(s) 171
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 2 cut(s) 500, 899
Mph1103I ATGCAT 1 cut(s) 586
MroNI GCCGGC 1 cut(s) 866
MroXI GAANNNNTTC 1 cut(s) 817
MseI TTAA 5 cut(s) 258, 390, 660, 701, 903
MslI CAYNNNNRTG 1 cut(s) 752
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 4 cut(s) 204, 708, 867, 971
MspR9I CCNGG 1 cut(s) 708
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 303
NaeI GCCGGC 1 cut(s) 868
NciI CCSGG 1 cut(s) 708
NdeI CATATG 1 cut(s) 580
NdeII GATC 4 cut(s) 136, 565, 803, 850
NgoMIV GCCGGC 1 cut(s) 866
NlaIII CATG 4 cut(s) 751, 757, 937, 956
NlaIV GGNNCC 3 cut(s) 567, 814, 865
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 1 cut(s) 937
NspV TTCGAA 1 cut(s) 99
PaeI GCATGC 1 cut(s) 937
PceI AGGCCT 1 cut(s) 714
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 868
PdmI GAANNNNTTC 1 cut(s) 817
PkrI GCNGC 6 cut(s) 93, 157, 308, 421, 528, 531
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
Psp124BI GAGCTC 2 cut(s) 55, 888
PspN4I GGNNCC 3 cut(s) 567, 814, 865
PspPI GGNCC 1 cut(s) 376
PsuI RGATCY 2 cut(s) 565, 850
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 752
SacI GAGCTC 2 cut(s) 55, 888
SaqAI TTAA 5 cut(s) 258, 390, 660, 701, 903
SatI GCNGC 6 cut(s) 92, 156, 307, 420, 527, 530
Sau3AI GATC 4 cut(s) 136, 565, 803, 850
Sau96I GGNCC 1 cut(s) 376
ScaI AGTACT 1 cut(s) 17
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 2 cut(s) 55, 888
SfaNI GCATC 3 cut(s) 284, 394, 657
SfcI CTRYAG 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SpeI ACTAGT 1 cut(s) 253
SphI GCATGC 1 cut(s) 937
Sse9I AATT 7 cut(s) 121, 209, 242, 259, 271, 571, 785
SseBI AGGCCT 1 cut(s) 714
SsiI CCGC 4 cut(s) 91, 153, 156, 297
SspI AATATT 1 cut(s) 324
SspMI CTAG 2 cut(s) 254, 836
SstI GAGCTC 2 cut(s) 55, 888
StuI AGGCCT 1 cut(s) 714
StyD4I CCNGG 1 cut(s) 706
TaaI ACNGT 6 cut(s) 182, 194, 313, 645, 844, 917
TaiI ACGT 2 cut(s) 473, 482
TaqI TCGA 5 cut(s) 33, 99, 502, 760, 888
TasI AATT 7 cut(s) 121, 209, 242, 259, 271, 571, 785
TatI WGTACW 1 cut(s) 15
TauI GCSGC 2 cut(s) 94, 158
Tru1I TTAA 5 cut(s) 258, 390, 660, 701, 903
Tru9I TTAA 5 cut(s) 258, 390, 660, 701, 903
TscAI CASTG 4 cut(s) 123, 376, 648, 915
TseI GCWGC 4 cut(s) 306, 419, 526, 529
TspDTI ATGAA 1 cut(s) 941
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 4 cut(s) 123, 376, 648, 915
XapI RAATTY 4 cut(s) 121, 209, 242, 271
XbaI TCTAGA 1 cut(s) 835
XceI RCATGY 1 cut(s) 937
XmnI GAANNNNTTC 1 cut(s) 817
XspI CTAG 2 cut(s) 254, 836
ZrmI AGTACT 1 cut(s) 17
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.