Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G55325 AT1G55325 AT1G55325 AT1G55325 |
| fragaria_vesca | FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 |
| malus_domestica | MD03G1004100.v1.1 |
| prunus_persica | Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 |
| pyrus_communis | pycom03g00420 |
| rosa_chinensis | RchiOBHm_Chr5g0083741 |
| rosa_laevigata | RLG00000037049 |
| rosa_multiflora | Rmu_sc0001287.1_g000006 |
| rosa_roxburghii | Rroxscaffold_1G00000840 |
| rosa_rugosa | Rorug05G0485500 Rorug05G0485600 Rorug05G0485700 |
| rosa_samantha | Rh5AG538600 Rh5BG564000 Rh5CG585900 Rh5DG572400 |
| rosa_wichuraiana | Rw5G050090 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 3280 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 5732 |
| AatII | GACGTC | 2 cut(s) | 1295, 5710 |
| Acc36I | ACCTGC | 3 cut(s) | 2608, 2843, 3280 |
| Acc65I | GGTACC | 1 cut(s) | 3288 |
| AccB1I | GGYRCC | 1 cut(s) | 3288 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 2887, 4265, 4334 |
| AccBSI | CCGCTC | 1 cut(s) | 1278 |
| AccI | GTMKAC | 4 cut(s) | 31, 810, 4101, 5381 |
| AccII | CGCG | 1 cut(s) | 1396 |
| AccIII | TCCGGA | 1 cut(s) | 4041 |
| AcoI | YGGCCR | 5 cut(s) | 672, 1391, 2845, 5167, 5453 |
| AcuI | CTGAAG | 8 cut(s) | 384, 799, 996, 1764, 3725, 4164, 5285, 5507 |
| AcyI | GRCGYC | 3 cut(s) | 274, 1292, 5707 |
| AfeI | AGCGCT | 3 cut(s) | 1264, 2718, 3723 |
| AflII | CTTAAG | 1 cut(s) | 3305 |
| AflIII | ACRYGT | 2 cut(s) | 1334, 5312 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 5640 |
| AjiI | CACGTC | 2 cut(s) | 1335, 2311 |
| AjuI | GAANNNNNNNTTGG | 8 cut(s) | 906, 938, 999, 1031, 3546, 3578, 4282, 4314 |
| AleI | CACNNNNGTG | 2 cut(s) | 2547, 4991 |
| Alw21I | GWGCWC | 4 cut(s) | 1605, 2554, 3984, 4715 |
| Alw44I | GTGCAC | 1 cut(s) | 2550 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1622, 3538, 4921 |
| Ama87I | CYCGRG | 1 cut(s) | 4499 |
| Aor13HI | TCCGGA | 1 cut(s) | 4041 |
| Aor51HI | AGCGCT | 3 cut(s) | 1264, 2718, 3723 |
| ApaLI | GTGCAC | 1 cut(s) | 2550 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1784, 1816, 3153, 3185 |
| AseI | ATTAAT | 1 cut(s) | 3378 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 171, 4775 |
| Asp718I | GGTACC | 1 cut(s) | 3288 |
| AspLEI | GCGC | 5 cut(s) | 1265, 2719, 3724, 4870, 5280 |
| AsuC2I | CCSGG | 2 cut(s) | 1581, 3398 |
| AvaI | CYCGRG | 1 cut(s) | 4499 |
| AvaII | GGWCC | 9 cut(s) | 186, 203, 879, 2780, 2834, 3011, 4523, 4826, 5550 |
| BaeGI | GKGCMC | 1 cut(s) | 2554 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1756, 1789 |
| BalI | TGGCCA | 4 cut(s) | 674, 2847, 5169, 5455 |
| BamHI | GGATCC | 1 cut(s) | 211 |
| BanI | GGYRCC | 1 cut(s) | 3288 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2649, 2681 |
| BauI | CACGAG | 1 cut(s) | 4125 |
| BbsI | GAAGAC | 4 cut(s) | 2664, 4170, 4476, 5702 |
| Bbv12I | GWGCWC | 4 cut(s) | 1605, 2554, 3984, 4715 |
| BbvCI | CCTCAGC | 2 cut(s) | 1089, 2928 |
| BceAI | ACGGC | 4 cut(s) | 1218, 3222, 5659, 5682 |
| BciVI | GTATCC | 5 cut(s) | 4078, 4297, 4591, 5164, 5736 |
| BclI | TGATCA | 2 cut(s) | 1441, 1825 |
| BcnI | CCSGG | 2 cut(s) | 1581, 3398 |
| BfaI | CTAG | 7 cut(s) | 2397, 2442, 2604, 2705, 4113, 4953, 5562 |
| BfmI | CTRYAG | 8 cut(s) | 1587, 1878, 2145, 2693, 3858, 4682, 4703, 4963 |
| BfoI | RGCGCY | 3 cut(s) | 1266, 2720, 3725 |
| BfrI | CTTAAG | 1 cut(s) | 3305 |
| BfuAI | ACCTGC | 3 cut(s) | 2608, 2843, 3280 |
| BfuI | GTATCC | 5 cut(s) | 4078, 4297, 4591, 5164, 5736 |
| BglII | AGATCT | 2 cut(s) | 1048, 4331 |
| BlpI | GCTNAGC | 2 cut(s) | 108, 2402 |
| BmcAI | AGTACT | 2 cut(s) | 2446, 5179 |
| Bme18I | GGWCC | 9 cut(s) | 186, 203, 879, 2780, 2834, 3011, 4523, 4826, 5550 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 5640 |
| BmeT110I | CYCGRG | 1 cut(s) | 4499 |
| BmgBI | CACGTC | 2 cut(s) | 1335, 2311 |
| BpiI | GAAGAC | 4 cut(s) | 2664, 4170, 4476, 5702 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1889, 1921, 2168, 2200, 4928, 4960 |
| BpmI | CTGGAG | 8 cut(s) | 616, 1303, 1599, 2532, 4356, 4663, 4842, 5722 |
| Bpu10I | CCTNAGC | 3 cut(s) | 300, 1089, 2928 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 108, 2402 |
| BpuEI | CTTGAG | 5 cut(s) | 204, 2091, 4064, 5413, 5615 |
| BpuMI | CCSGG | 2 cut(s) | 1581, 3398 |
| BsaAI | YACGTR | 2 cut(s) | 398, 910 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2047, 3405 |
| BsaHI | GRCGYC | 3 cut(s) | 274, 1292, 5707 |
| BsaI | GGTCTC | 1 cut(s) | 860 |
| BsaWI | WCCGGW | 1 cut(s) | 4041 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1846, 1876 |
| Bse3DI | GCAATG | 5 cut(s) | 901, 1854, 2892, 4615, 5669 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2047, 3405 |
| BseAI | TCCGGA | 1 cut(s) | 4041 |
| BseJI | GATNNNNATC | 2 cut(s) | 2047, 3405 |
| BseMI | GCAATG | 5 cut(s) | 901, 1854, 2892, 4615, 5669 |
| BseRI | GAGGAG | 4 cut(s) | 505, 741, 4705, 5559 |
| BseSI | GKGCMC | 1 cut(s) | 2554 |
| BseX3I | CGGCCG | 1 cut(s) | 1391 |
| BseYI | CCCAGC | 2 cut(s) | 3514, 4584 |
| Bsh1236I | CGCG | 1 cut(s) | 1396 |
| Bsh1285I | CGRYCG | 3 cut(s) | 1394, 3248, 3846 |
| BshNI | GGYRCC | 1 cut(s) | 3288 |
| BsiEI | CGRYCG | 3 cut(s) | 1394, 3248, 3846 |
| BsiHKAI | GWGCWC | 4 cut(s) | 1605, 2554, 3984, 4715 |
| BsiHKCI | CYCGRG | 1 cut(s) | 4499 |
| BsiSI | CCGG | 3 cut(s) | 1580, 3398, 4042 |
| BslFI | GGGAC | 9 cut(s) | 371, 510, 771, 865, 1626, 2567, 2820, 3983, 5563 |
| BsmBI | CGTCTC | 3 cut(s) | 2809, 3197, 5698 |
| BsmFI | GGGAC | 9 cut(s) | 371, 510, 771, 865, 1626, 2567, 2820, 3983, 5563 |
| BsmI | GAATGC | 3 cut(s) | 765, 2224, 4137 |
| Bso31I | GGTCTC | 1 cut(s) | 860 |
| BsoBI | CYCGRG | 1 cut(s) | 4499 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1605, 2554, 3984, 4715 |
| Bsp13I | TCCGGA | 1 cut(s) | 4041 |
| Bsp1407I | TGTACA | 2 cut(s) | 2725, 3798 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 108, 2402 |
| BspEI | TCCGGA | 1 cut(s) | 4041 |
| BspFNI | CGCG | 1 cut(s) | 1396 |
| BspHI | TCATGA | 1 cut(s) | 5509 |
| BspMAI | CTGCAG | 3 cut(s) | 1591, 2697, 4686 |
| BspMI | ACCTGC | 3 cut(s) | 2608, 2843, 3280 |
| BspT107I | GGYRCC | 1 cut(s) | 3288 |
| BspTI | CTTAAG | 1 cut(s) | 3305 |
| BspTNI | GGTCTC | 1 cut(s) | 860 |
| BsrBI | CCGCTC | 1 cut(s) | 1278 |
| BsrDI | GCAATG | 5 cut(s) | 901, 1854, 2892, 4615, 5669 |
| BsrGI | TGTACA | 2 cut(s) | 2725, 3798 |
| BssNAI | GTATAC | 1 cut(s) | 4102 |
| BssNI | GRCGYC | 3 cut(s) | 274, 1292, 5707 |
| BssSI | CACGAG | 1 cut(s) | 4125 |
| BssT1I | CCWWGG | 3 cut(s) | 206, 1165, 5063 |
| Bst1107I | GTATAC | 1 cut(s) | 4102 |
| Bst2BI | CACGAG | 1 cut(s) | 4125 |
| Bst6I | CTCTTC | 6 cut(s) | 360, 528, 1813, 2799, 2961, 5304 |
| BstACI | GRCGYC | 3 cut(s) | 274, 1292, 5707 |
| BstAFI | CTTAAG | 1 cut(s) | 3305 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 903, 2600, 2692 |
| BstAUI | TGTACA | 2 cut(s) | 2725, 3798 |
| BstBAI | YACGTR | 2 cut(s) | 398, 910 |
| BstC8I | GCNNGC | 8 cut(s) | 2023, 2071, 2301, 2733, 2792, 2796, 2845, 4711 |
| BstDSI | CCRYGG | 1 cut(s) | 1644 |
| BstFNI | CGCG | 1 cut(s) | 1396 |
| BstH2I | RGCGCY | 3 cut(s) | 1266, 2720, 3725 |
| BstHHI | GCGC | 5 cut(s) | 1265, 2719, 3724, 4870, 5280 |
| BstMCI | CGRYCG | 3 cut(s) | 1394, 3248, 3846 |
| BstNSI | RCATGY | 3 cut(s) | 1601, 5278, 5316 |
| BstSFI | CTRYAG | 8 cut(s) | 1587, 1878, 2145, 2693, 3858, 4682, 4703, 4963 |
| BstSLI | GKGCMC | 1 cut(s) | 2554 |
| BstSNI | TACGTA | 1 cut(s) | 398 |
| BstUI | CGCG | 1 cut(s) | 1396 |
| BstV2I | GAAGAC | 4 cut(s) | 2664, 4170, 4476, 5702 |
| BstX2I | RGATCY | 9 cut(s) | 211, 1048, 2698, 3065, 3647, 4331, 4730, 4898, 4918 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1645, 3446 |
| BstYI | RGATCY | 9 cut(s) | 211, 1048, 2698, 3065, 3647, 4331, 4730, 4898, 4918 |
| BstZ17I | GTATAC | 1 cut(s) | 4102 |
| BstZI | CGGCCG | 1 cut(s) | 1391 |
| BsuI | GTATCC | 5 cut(s) | 4078, 4297, 4591, 5164, 5736 |
| BtgI | CCRYGG | 1 cut(s) | 1644 |
| BtgZI | GCGATG | 2 cut(s) | 609, 3621 |
| BtrI | CACGTC | 2 cut(s) | 1335, 2311 |
| BtsI | GCAGTG | 3 cut(s) | 1101, 1228, 2690 |
| BtsIMutI | CAGTG | 9 cut(s) | 1004, 1101, 1228, 2004, 2161, 2690, 3984, 5084, 5680 |
| BveI | ACCTGC | 3 cut(s) | 2608, 2843, 3280 |
| Cac8I | GCNNGC | 8 cut(s) | 2023, 2071, 2301, 2733, 2792, 2796, 2845, 4711 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1622, 3538, 4921 |
| CciI | TCATGA | 1 cut(s) | 5509 |
| CciNI | GCGGCCGC | 1 cut(s) | 1391 |
| CfoI | GCGC | 5 cut(s) | 1265, 2719, 3724, 4870, 5280 |
| CseI | GACGC | 4 cut(s) | 263, 321, 1161, 1322 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1204, 1239, 2292, 2327 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 5732 |
| DriI | GACNNNNNGTC | 1 cut(s) | 5640 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 5732 |
| EaeI | YGGCCR | 5 cut(s) | 672, 1391, 2845, 5167, 5453 |
| EagI | CGGCCG | 1 cut(s) | 1391 |
| Eam1104I | CTCTTC | 6 cut(s) | 360, 528, 1813, 2799, 2961, 5304 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 5640 |
| EarI | CTCTTC | 6 cut(s) | 360, 528, 1813, 2799, 2961, 5304 |
| EciI | GGCGGA | 2 cut(s) | 2584, 5677 |
| EclXI | CGGCCG | 1 cut(s) | 1391 |
| Eco105I | TACGTA | 1 cut(s) | 398 |
| Eco130I | CCWWGG | 3 cut(s) | 206, 1165, 5063 |
| Eco147I | AGGCCT | 2 cut(s) | 721, 3750 |
| Eco31I | GGTCTC | 1 cut(s) | 860 |
| Eco32I | GATATC | 3 cut(s) | 372, 1504, 3312 |
| Eco47I | GGWCC | 9 cut(s) | 186, 203, 879, 2780, 2834, 3011, 4523, 4826, 5550 |
| Eco47III | AGCGCT | 3 cut(s) | 1264, 2718, 3723 |
| Eco52I | CGGCCG | 1 cut(s) | 1391 |
| Eco57I | CTGAAG | 8 cut(s) | 384, 799, 996, 1764, 3725, 4164, 5285, 5507 |
| Eco88I | CYCGRG | 1 cut(s) | 4499 |
| EcoO109I | RGGNCCY | 3 cut(s) | 879, 3343, 4826 |
| EcoRI | GAATTC | 2 cut(s) | 512, 4534 |
| EcoRV | GATATC | 3 cut(s) | 372, 1504, 3312 |
| EcoT14I | CCWWGG | 3 cut(s) | 206, 1165, 5063 |
| EcoT22I | ATGCAT | 2 cut(s) | 2057, 4147 |
| ErhI | CCWWGG | 3 cut(s) | 206, 1165, 5063 |
| Esp3I | CGTCTC | 3 cut(s) | 2809, 3197, 5698 |
| FaqI | GGGAC | 9 cut(s) | 371, 510, 771, 865, 1626, 2567, 2820, 3983, 5563 |
| FauI | CCCGC | 2 cut(s) | 756, 1265 |
| FauNDI | CATATG | 1 cut(s) | 3358 |
| FbaI | TGATCA | 2 cut(s) | 1441, 1825 |
| FblI | GTMKAC | 4 cut(s) | 31, 810, 4101, 5381 |
| FspBI | CTAG | 7 cut(s) | 2397, 2442, 2604, 2705, 4113, 4953, 5562 |
| GlaI | GCGC | 5 cut(s) | 1264, 2718, 3723, 4869, 5279 |
| GsaI | CCCAGC | 2 cut(s) | 3518, 4588 |
| GsuI | CTGGAG | 8 cut(s) | 616, 1303, 1599, 2532, 4356, 4663, 4842, 5722 |
| HaeII | RGCGCY | 3 cut(s) | 1266, 2720, 3725 |
| HapII | CCGG | 3 cut(s) | 1580, 3398, 4042 |
| HgaI | GACGC | 4 cut(s) | 263, 321, 1161, 1322 |
| HhaI | GCGC | 5 cut(s) | 1265, 2719, 3724, 4870, 5280 |
| Hin1I | GRCGYC | 3 cut(s) | 274, 1292, 5707 |
| Hin6I | GCGC | 5 cut(s) | 1263, 2717, 3722, 4868, 5278 |
| HinP1I | GCGC | 5 cut(s) | 1263, 2717, 3722, 4868, 5278 |
| HincII | GTYRAC | 4 cut(s) | 982, 3462, 3952, 4188 |
| HindII | GTYRAC | 4 cut(s) | 982, 3462, 3952, 4188 |
| HindIII | AAGCTT | 6 cut(s) | 251, 317, 2331, 2906, 3737, 4057 |
| HpaI | GTTAAC | 1 cut(s) | 982 |
| HpaII | CCGG | 3 cut(s) | 1580, 3398, 4042 |
| Hpy99I | CGWCG | 3 cut(s) | 1912, 3851, 4863 |
| Hsp92I | GRCGYC | 3 cut(s) | 274, 1292, 5707 |
| HspAI | GCGC | 5 cut(s) | 1263, 2717, 3722, 4868, 5278 |
| KflI | GGGWCCC | 1 cut(s) | 879 |
| Kpn2I | TCCGGA | 1 cut(s) | 4041 |
| KpnI | GGTACC | 1 cut(s) | 3292 |
| Ksp22I | TGATCA | 2 cut(s) | 1441, 1825 |
| KspAI | GTTAAC | 1 cut(s) | 982 |
| MaeI | CTAG | 7 cut(s) | 2397, 2442, 2604, 2705, 4113, 4953, 5562 |
| MaeIII | GTNAC | 6 cut(s) | 916, 1211, 1538, 2156, 3022, 5328 |
| MbiI | CCGCTC | 1 cut(s) | 1278 |
| MfeI | CAATTG | 1 cut(s) | 5601 |
| MflI | RGATCY | 9 cut(s) | 211, 1048, 2698, 3065, 3647, 4331, 4730, 4898, 4918 |
| MhlI | GDGCHC | 4 cut(s) | 1605, 2554, 3984, 4715 |
| MlsI | TGGCCA | 4 cut(s) | 674, 2847, 5169, 5455 |
| MluNI | TGGCCA | 4 cut(s) | 674, 2847, 5169, 5455 |
| MmeI | TCCRAC | 5 cut(s) | 181, 2514, 3268, 4610, 5333 |
| Mox20I | TGGCCA | 4 cut(s) | 674, 2847, 5169, 5455 |
| Mph1103I | ATGCAT | 2 cut(s) | 2057, 4147 |
| MroI | TCCGGA | 1 cut(s) | 4041 |
| MroXI | GAANNNNTTC | 2 cut(s) | 171, 4775 |
| MscI | TGGCCA | 4 cut(s) | 674, 2847, 5169, 5455 |
| MslI | CAYNNNNRTG | 3 cut(s) | 2547, 3977, 4991 |
| Msp20I | TGGCCA | 4 cut(s) | 674, 2847, 5169, 5455 |
| MspA1I | CMGCKG | 7 cut(s) | 1088, 1272, 2428, 4820, 5018, 5144, 5159 |
| MspCI | CTTAAG | 1 cut(s) | 3305 |
| MspI | CCGG | 3 cut(s) | 1580, 3398, 4042 |
| MunI | CAATTG | 1 cut(s) | 5601 |
| Mva1269I | GAATGC | 3 cut(s) | 765, 2224, 4137 |
| MvnI | CGCG | 1 cut(s) | 1396 |
| NciI | CCSGG | 2 cut(s) | 1581, 3398 |
| NdeI | CATATG | 1 cut(s) | 3358 |
| NmeAIII | GCCGAG | 1 cut(s) | 5350 |
| NmuCI | GTSAC | 3 cut(s) | 1538, 2156, 5328 |
| NotI | GCGGCCGC | 1 cut(s) | 1391 |
| NsiI | ATGCAT | 2 cut(s) | 2057, 4147 |
| NspI | RCATGY | 3 cut(s) | 1601, 5278, 5316 |
| OliI | CACNNNNGTG | 2 cut(s) | 2547, 4991 |
| PagI | TCATGA | 1 cut(s) | 5509 |
| PaqCI | CACCTGC | 1 cut(s) | 3280 |
| PasI | CCCWGGG | 2 cut(s) | 4570, 4743 |
| PceI | AGGCCT | 2 cut(s) | 721, 3750 |
| PciI | ACATGT | 1 cut(s) | 5312 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 880, 1124, 1808, 4181, 5672 |
| PctI | GAATGC | 3 cut(s) | 765, 2224, 4137 |
| PdmI | GAANNNNTTC | 2 cut(s) | 171, 4775 |
| PflFI | GACNNNGTC | 1 cut(s) | 797 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 2887, 4265, 4334 |
| PfoI | TCCNGGA | 1 cut(s) | 5738 |
| Ple19I | CGATCG | 1 cut(s) | 3846 |
| Ppu21I | YACGTR | 2 cut(s) | 398, 910 |
| PpuMI | RGGWCCY | 2 cut(s) | 879, 4826 |
| PscI | ACATGT | 1 cut(s) | 5312 |
| PshBI | ATTAAT | 1 cut(s) | 3378 |
| Psp5II | RGGWCCY | 2 cut(s) | 879, 4826 |
| PspFI | CCCAGC | 2 cut(s) | 3514, 4584 |
| PspPPI | RGGWCCY | 2 cut(s) | 879, 4826 |
| PstI | CTGCAG | 3 cut(s) | 1591, 2697, 4686 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1622, 3538, 4921 |
| PsuI | RGATCY | 9 cut(s) | 211, 1048, 2698, 3065, 3647, 4331, 4730, 4898, 4918 |
| PsyI | GACNNNGTC | 1 cut(s) | 797 |
| PvuI | CGATCG | 1 cut(s) | 3846 |
| PvuII | CAGCTG | 4 cut(s) | 1088, 2428, 4820, 5144 |
| RseI | CAYNNNNRTG | 3 cut(s) | 2547, 3977, 4991 |
| ScaI | AGTACT | 2 cut(s) | 2446, 5179 |
| SduI | GDGCHC | 4 cut(s) | 1605, 2554, 3984, 4715 |
| SfcI | CTRYAG | 8 cut(s) | 1587, 1878, 2145, 2693, 3858, 4682, 4703, 4963 |
| SinI | GGWCC | 9 cut(s) | 186, 203, 879, 2780, 2834, 3011, 4523, 4826, 5550 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 2547, 3977, 4991 |
| SmlI | CTYRAG | 6 cut(s) | 219, 2106, 3305, 4079, 5428, 5594 |
| SmoI | CTYRAG | 6 cut(s) | 219, 2106, 3305, 4079, 5428, 5594 |
| SnaBI | TACGTA | 1 cut(s) | 398 |
| SseBI | AGGCCT | 2 cut(s) | 721, 3750 |
| SspI | AATATT | 3 cut(s) | 485, 2617, 3152 |
| SspMI | CTAG | 7 cut(s) | 2397, 2442, 2604, 2705, 4113, 4953, 5562 |
| StuI | AGGCCT | 2 cut(s) | 721, 3750 |
| StyI | CCWWGG | 3 cut(s) | 206, 1165, 5063 |
| TaqII | GACCGA | 2 cut(s) | 1409, 3262 |
| TatI | WGTACW | 6 cut(s) | 136, 2168, 2444, 2725, 3798, 5177 |
| TauI | GCSGC | 5 cut(s) | 1281, 1393, 1396, 2353, 5609 |
| TscAI | CASTG | 9 cut(s) | 1004, 1101, 1228, 2011, 2161, 2697, 3984, 5084, 5687 |
| TseFI | GTSAC | 3 cut(s) | 1538, 2156, 5328 |
| Tsp45I | GTSAC | 3 cut(s) | 1538, 2156, 5328 |
| TspGWI | ACGGA | 4 cut(s) | 2538, 4682, 4911, 5182 |
| TspRI | CASTG | 9 cut(s) | 1004, 1101, 1228, 2011, 2161, 2697, 3984, 5084, 5687 |
| Tth111I | GACNNNGTC | 1 cut(s) | 797 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 2887, 4265, 4334 |
| Vha464I | CTTAAG | 1 cut(s) | 3305 |
| VneI | GTGCAC | 1 cut(s) | 2550 |
| VpaK11BI | GGWCC | 9 cut(s) | 186, 203, 879, 2780, 2834, 3011, 4523, 4826, 5550 |
| VspI | ATTAAT | 1 cut(s) | 3378 |
| XceI | RCATGY | 3 cut(s) | 1601, 5278, 5316 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 2562, 2855, 5177 |
| XmiI | GTMKAC | 4 cut(s) | 31, 810, 4101, 5381 |
| XmnI | GAANNNNTTC | 2 cut(s) | 171, 4775 |
| XspI | CTAG | 7 cut(s) | 2397, 2442, 2604, 2705, 4113, 4953, 5562 |
| ZraI | GACGTC | 2 cut(s) | 1293, 5708 |
| ZrmI | AGTACT | 2 cut(s) | 2446, 5179 |
| Zsp2I | ATGCAT | 2 cut(s) | 2057, 4147 |