Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G55325 AT1G55325 AT1G55325 AT1G55325 |
| fragaria_vesca | FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 |
| malus_domestica | MD03G1004100.v1.1 |
| prunus_persica | Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 |
| pyrus_communis | pycom03g00420 |
| rosa_chinensis | RchiOBHm_Chr5g0083741 |
| rosa_laevigata | RLG00000037049 |
| rosa_multiflora | Rmu_sc0001287.1_g000006 |
| rosa_roxburghii | Rroxscaffold_1G00000840 |
| rosa_rugosa | Rorug05G0485500 Rorug05G0485600 Rorug05G0485700 |
| rosa_samantha | Rh5AG538600 Rh5BG564000 Rh5CG585900 Rh5DG572400 |
| rosa_wichuraiana | Rw5G050090 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 3 cut(s) | 2873, 3294, 4780 |
| AccB1I | GGYRCC | 2 cut(s) | 1541, 1774 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1670 |
| AccBSI | CCGCTC | 1 cut(s) | 1661 |
| AccI | GTMKAC | 4 cut(s) | 1297, 1683, 3776, 4723 |
| AccII | CGCG | 1 cut(s) | 3301 |
| AciI | CCGC | 5 cut(s) | 1659, 3073, 3301, 3486, 4436 |
| AcoI | YGGCCR | 1 cut(s) | 4337 |
| AcsI | RAATTY | 5 cut(s) | 506, 622, 1871, 2098, 2332 |
| AcuI | CTGAAG | 6 cut(s) | 626, 868, 1836, 2528, 2708, 3746 |
| AcvI | CACGTG | 1 cut(s) | 2234 |
| AdeI | CACNNNGTG | 2 cut(s) | 470, 1166 |
| AflII | CTTAAG | 2 cut(s) | 3368, 4103 |
| AflIII | ACRYGT | 3 cut(s) | 2031, 2231, 4410 |
| AhlI | ACTAGT | 1 cut(s) | 4073 |
| AjiI | CACGTC | 1 cut(s) | 772 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 813, 845, 935, 967 |
| AleI | CACNNNNGTG | 1 cut(s) | 1323 |
| Alw21I | GWGCWC | 2 cut(s) | 577, 2614 |
| Alw26I | GTCTC | 7 cut(s) | 778, 1781, 1957, 2626, 3230, 3735, 4103 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 385, 1631, 1670, 1838, 4786 |
| Ama87I | CYCGRG | 2 cut(s) | 1225, 2616 |
| ApaI | GGGCCC | 1 cut(s) | 1074 |
| ApoI | RAATTY | 5 cut(s) | 506, 622, 1871, 2098, 2332 |
| AseI | ATTAAT | 1 cut(s) | 2750 |
| AspLEI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4644 |
| AsuC2I | CCSGG | 4 cut(s) | 227, 261, 4233, 4270 |
| AsuII | TTCGAA | 1 cut(s) | 1749 |
| AvaI | CYCGRG | 2 cut(s) | 1225, 2616 |
| AvaII | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| BaeGI | GKGCMC | 2 cut(s) | 1074, 3833 |
| BalI | TGGCCA | 1 cut(s) | 4339 |
| BamHI | GGATCC | 2 cut(s) | 202, 3890 |
| BanI | GGYRCC | 2 cut(s) | 1541, 1774 |
| BanII | GRGCYC | 5 cut(s) | 1074, 1623, 2614, 3185, 3671 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 2188, 2220, 2887, 2919 |
| BauI | CACGAG | 2 cut(s) | 65, 4149 |
| BbrPI | CACGTG | 1 cut(s) | 2234 |
| BbsI | GAAGAC | 4 cut(s) | 2087, 2418, 3892, 4212 |
| Bbv12I | GWGCWC | 2 cut(s) | 577, 2614 |
| BbvCI | CCTCAGC | 1 cut(s) | 996 |
| BceAI | ACGGC | 2 cut(s) | 2426, 3024 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1535, 1569 |
| BciVI | GTATCC | 3 cut(s) | 1420, 2400, 4549 |
| BclI | TGATCA | 4 cut(s) | 78, 937, 1714, 1876 |
| BcnI | CCSGG | 4 cut(s) | 227, 261, 4233, 4270 |
| BcoDI | GTCTC | 7 cut(s) | 778, 1781, 1957, 2626, 3230, 3735, 4103 |
| BcuI | ACTAGT | 1 cut(s) | 4073 |
| BfaI | CTAG | 8 cut(s) | 726, 1268, 1992, 2643, 3509, 3662, 4074, 4305 |
| BfoI | RGCGCY | 3 cut(s) | 1363, 2602, 4645 |
| BfrI | CTTAAG | 2 cut(s) | 3368, 4103 |
| BfuAI | ACCTGC | 3 cut(s) | 2873, 3294, 4780 |
| BfuI | GTATCC | 3 cut(s) | 1420, 2400, 4549 |
| BglII | AGATCT | 1 cut(s) | 3923 |
| BmcAI | AGTACT | 3 cut(s) | 603, 3151, 4005 |
| Bme18I | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| BmeT110I | CYCGRG | 2 cut(s) | 1225, 2616 |
| BmgBI | CACGTC | 1 cut(s) | 772 |
| BmiI | GGNNCC | 9 cut(s) | 204, 1072, 1258, 1543, 1620, 1776, 3408, 3892, 4440 |
| BpiI | GAAGAC | 4 cut(s) | 2087, 2418, 3892, 4212 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1021, 1053, 3566, 3598 |
| BpmI | CTGGAG | 3 cut(s) | 348, 1647, 4763 |
| Bpu10I | CCTNAGC | 2 cut(s) | 996, 3840 |
| Bpu14I | TTCGAA | 1 cut(s) | 1749 |
| BpuEI | CTTGAG | 7 cut(s) | 276, 762, 1377, 2617, 3434, 3612, 4557 |
| BpuMI | CCSGG | 4 cut(s) | 227, 261, 4233, 4270 |
| Bsa29I | ATCGAT | 1 cut(s) | 3468 |
| BsaAI | YACGTR | 2 cut(s) | 2034, 2234 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2571 |
| BsaI | GGTCTC | 1 cut(s) | 2626 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1897, 1927 |
| Bse3DI | GCAATG | 4 cut(s) | 2922, 3329, 3639, 3658 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2571 |
| BseCI | ATCGAT | 1 cut(s) | 3468 |
| BseJI | GATNNNNATC | 1 cut(s) | 2571 |
| BseMI | GCAATG | 4 cut(s) | 2922, 3329, 3639, 3658 |
| BseRI | GAGGAG | 2 cut(s) | 645, 2604 |
| BseSI | GKGCMC | 2 cut(s) | 1074, 3833 |
| BseYI | CCCAGC | 2 cut(s) | 1694, 4367 |
| BsgI | GTGCAG | 2 cut(s) | 1347, 3374 |
| Bsh1236I | CGCG | 1 cut(s) | 3301 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1324 |
| BshNI | GGYRCC | 2 cut(s) | 1541, 1774 |
| BshVI | ATCGAT | 1 cut(s) | 3468 |
| BsiEI | CGRYCG | 1 cut(s) | 1324 |
| BsiHKAI | GWGCWC | 2 cut(s) | 577, 2614 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1225, 2616 |
| BsiSI | CCGG | 5 cut(s) | 227, 260, 1772, 4232, 4269 |
| BslFI | GGGAC | 6 cut(s) | 362, 995, 2329, 3722, 4752, 4841 |
| BsmAI | GTCTC | 7 cut(s) | 778, 1781, 1957, 2626, 3230, 3735, 4103 |
| BsmBI | CGTCTC | 1 cut(s) | 778 |
| BsmFI | GGGAC | 6 cut(s) | 362, 995, 2329, 3722, 4752, 4841 |
| BsmI | GAATGC | 3 cut(s) | 610, 2381, 4273 |
| Bso31I | GGTCTC | 1 cut(s) | 2626 |
| BsoBI | CYCGRG | 2 cut(s) | 1225, 2616 |
| Bsp119I | TTCGAA | 1 cut(s) | 1749 |
| Bsp120I | GGGCCC | 1 cut(s) | 1070 |
| Bsp1286I | GDGCHC | 7 cut(s) | 577, 1074, 1623, 2614, 3185, 3671, 3833 |
| Bsp1407I | TGTACA | 1 cut(s) | 2755 |
| Bsp19I | CCATGG | 2 cut(s) | 1779, 3810 |
| BspACI | CCGC | 5 cut(s) | 1659, 3073, 3301, 3486, 4436 |
| BspDI | ATCGAT | 1 cut(s) | 3468 |
| BspFNI | CGCG | 1 cut(s) | 3301 |
| BspLI | GGNNCC | 9 cut(s) | 204, 1072, 1258, 1543, 1620, 1776, 3408, 3892, 4440 |
| BspMAI | CTGCAG | 8 cut(s) | 1059, 1636, 2994, 3291, 4041, 4351, 4770, 4791 |
| BspMI | ACCTGC | 3 cut(s) | 2873, 3294, 4780 |
| BspQI | GCTCTTC | 3 cut(s) | 468, 1855, 4804 |
| BspT104I | TTCGAA | 1 cut(s) | 1749 |
| BspT107I | GGYRCC | 2 cut(s) | 1541, 1774 |
| BspTI | CTTAAG | 2 cut(s) | 3368, 4103 |
| BspTNI | GGTCTC | 1 cut(s) | 2626 |
| BsrBI | CCGCTC | 1 cut(s) | 1661 |
| BsrDI | GCAATG | 4 cut(s) | 2922, 3329, 3639, 3658 |
| BsrGI | TGTACA | 1 cut(s) | 2755 |
| BssSI | CACGAG | 2 cut(s) | 65, 4149 |
| BssT1I | CCWWGG | 4 cut(s) | 197, 1779, 3810, 4873 |
| Bst2BI | CACGAG | 2 cut(s) | 65, 4149 |
| Bst6I | CTCTTC | 8 cut(s) | 351, 468, 522, 925, 1779, 1855, 2504, 4804 |
| BstAFI | CTTAAG | 2 cut(s) | 3368, 4103 |
| BstAUI | TGTACA | 1 cut(s) | 2755 |
| BstBAI | YACGTR | 2 cut(s) | 2034, 2234 |
| BstBI | TTCGAA | 1 cut(s) | 1749 |
| BstDSI | CCRYGG | 3 cut(s) | 1779, 3093, 3810 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| BstFNI | CGCG | 1 cut(s) | 3301 |
| BstH2I | RGCGCY | 3 cut(s) | 1363, 2602, 4645 |
| BstHHI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4644 |
| BstMAI | GTCTC | 7 cut(s) | 778, 1781, 1957, 2626, 3230, 3735, 4103 |
| BstMCI | CGRYCG | 1 cut(s) | 1324 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 256, 296, 1078, 1209, 1218, 1256, 2288, 3072, 3749 |
| BstNSI | RCATGY | 7 cut(s) | 220, 548, 569, 1556, 2112, 3531, 4414 |
| BstSLI | GKGCMC | 2 cut(s) | 1074, 3833 |
| BstUI | CGCG | 1 cut(s) | 3301 |
| BstV2I | GAAGAC | 4 cut(s) | 2087, 2418, 3892, 4212 |
| BstX2I | RGATCY | 8 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154, 4193 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 124, 4375 |
| BstYI | RGATCY | 8 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154, 4193 |
| Bsu15I | ATCGAT | 1 cut(s) | 3468 |
| BsuI | GTATCC | 3 cut(s) | 1420, 2400, 4549 |
| BsuTUI | ATCGAT | 1 cut(s) | 3468 |
| BtgI | CCRYGG | 3 cut(s) | 1779, 3093, 3810 |
| BtgZI | GCGATG | 1 cut(s) | 1689 |
| BtrI | CACGTC | 1 cut(s) | 772 |
| BtsI | GCAGTG | 6 cut(s) | 106, 306, 1008, 2179, 2846, 3978 |
| BveI | ACCTGC | 3 cut(s) | 2873, 3294, 4780 |
| CaiI | CAGNNNCTG | 5 cut(s) | 385, 1631, 1670, 1838, 4786 |
| CfoI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4644 |
| ClaI | ATCGAT | 1 cut(s) | 3468 |
| CseI | GACGC | 2 cut(s) | 1286, 4310 |
| CsiI | ACCWGGT | 2 cut(s) | 844, 4441 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2822, 2857 |
| DraIII | CACNNNGTG | 2 cut(s) | 470, 1166 |
| EaeI | YGGCCR | 1 cut(s) | 4337 |
| Eam1104I | CTCTTC | 8 cut(s) | 351, 468, 522, 925, 1779, 1855, 2504, 4804 |
| EarI | CTCTTC | 8 cut(s) | 351, 468, 522, 925, 1779, 1855, 2504, 4804 |
| Ecl136II | GAGCTC | 1 cut(s) | 2612 |
| Eco130I | CCWWGG | 4 cut(s) | 197, 1779, 3810, 4873 |
| Eco24I | GRGCYC | 5 cut(s) | 1074, 1623, 2614, 3185, 3671 |
| Eco31I | GGTCTC | 1 cut(s) | 2626 |
| Eco32I | GATATC | 1 cut(s) | 339 |
| Eco47I | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| Eco53kI | GAGCTC | 1 cut(s) | 2612 |
| Eco57I | CTGAAG | 6 cut(s) | 626, 868, 1836, 2528, 2708, 3746 |
| Eco72I | CACGTG | 1 cut(s) | 2234 |
| Eco88I | CYCGRG | 2 cut(s) | 1225, 2616 |
| EcoICRI | GAGCTC | 1 cut(s) | 2612 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| EcoO109I | RGGNCCY | 4 cut(s) | 858, 1070, 1071, 1835 |
| EcoRI | GAATTC | 1 cut(s) | 2098 |
| EcoRV | GATATC | 1 cut(s) | 339 |
| EcoT14I | CCWWGG | 4 cut(s) | 197, 1779, 3810, 4873 |
| EcoT22I | ATGCAT | 2 cut(s) | 3658, 4658 |
| EcoT38I | GRGCYC | 5 cut(s) | 1074, 1623, 2614, 3185, 3671 |
| ErhI | CCWWGG | 4 cut(s) | 197, 1779, 3810, 4873 |
| Esp3I | CGTCTC | 1 cut(s) | 778 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 813, 845, 1402, 1434, 2996, 3028 |
| FaqI | GGGAC | 6 cut(s) | 362, 995, 2329, 3722, 4752, 4841 |
| FauNDI | CATATG | 1 cut(s) | 3421 |
| FbaI | TGATCA | 4 cut(s) | 78, 937, 1714, 1876 |
| FblI | GTMKAC | 4 cut(s) | 1297, 1683, 3776, 4723 |
| FriOI | GRGCYC | 5 cut(s) | 1074, 1623, 2614, 3185, 3671 |
| FspBI | CTAG | 8 cut(s) | 726, 1268, 1992, 2643, 3509, 3662, 4074, 4305 |
| GlaI | GCGC | 4 cut(s) | 1361, 2583, 2600, 4643 |
| GsaI | CCCAGC | 2 cut(s) | 1698, 4371 |
| GsuI | CTGGAG | 3 cut(s) | 348, 1647, 4763 |
| HaeII | RGCGCY | 3 cut(s) | 1363, 2602, 4645 |
| HapII | CCGG | 5 cut(s) | 227, 260, 1772, 4232, 4269 |
| HgaI | GACGC | 2 cut(s) | 1286, 4310 |
| HhaI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4644 |
| Hin6I | GCGC | 4 cut(s) | 1360, 2582, 2599, 4642 |
| HinP1I | GCGC | 4 cut(s) | 1360, 2582, 2599, 4642 |
| HincII | GTYRAC | 4 cut(s) | 1462, 1684, 2940, 3580 |
| HindII | GTYRAC | 4 cut(s) | 1462, 1684, 2940, 3580 |
| HindIII | AAGCTT | 1 cut(s) | 3750 |
| HpaI | GTTAAC | 1 cut(s) | 2940 |
| HpaII | CCGG | 5 cut(s) | 227, 260, 1772, 4232, 4269 |
| Hpy99I | CGWCG | 1 cut(s) | 4143 |
| HpyCH4IV | ACGT | 5 cut(s) | 771, 1165, 2033, 2233, 3929 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 256, 296, 1078, 1209, 1218, 1256, 2288, 3072, 3749 |
| HpySE526I | ACGT | 5 cut(s) | 771, 1165, 2033, 2233, 3929 |
| HspAI | GCGC | 4 cut(s) | 1360, 2582, 2599, 4642 |
| Ksp22I | TGATCA | 4 cut(s) | 78, 937, 1714, 1876 |
| KspAI | GTTAAC | 1 cut(s) | 2940 |
| LguI | GCTCTTC | 3 cut(s) | 468, 1855, 4804 |
| LmnI | GCTCC | 9 cut(s) | 1256, 1618, 1666, 1918, 1925, 2609, 2617, 3331, 3412 |
| MabI | ACCWGGT | 2 cut(s) | 844, 4441 |
| MaeI | CTAG | 8 cut(s) | 726, 1268, 1992, 2643, 3509, 3662, 4074, 4305 |
| MaeII | ACGT | 5 cut(s) | 771, 1165, 2033, 2233, 3929 |
| MaeIII | GTNAC | 7 cut(s) | 823, 851, 1532, 2909, 3052, 4045, 4069 |
| MbiI | CCGCTC | 1 cut(s) | 1661 |
| MfeI | CAATTG | 1 cut(s) | 501 |
| MflI | RGATCY | 8 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154, 4193 |
| MhlI | GDGCHC | 7 cut(s) | 577, 1074, 1623, 2614, 3185, 3671, 3833 |
| MlsI | TGGCCA | 1 cut(s) | 4339 |
| MluNI | TGGCCA | 1 cut(s) | 4339 |
| MlyI | GAGTC | 9 cut(s) | 77, 278, 344, 1818, 2318, 2786, 3125, 3575, 4594 |
| MmeI | TCCRAC | 5 cut(s) | 51, 1542, 1792, 3015, 3178 |
| Mox20I | TGGCCA | 1 cut(s) | 4339 |
| Mph1103I | ATGCAT | 2 cut(s) | 3658, 4658 |
| MscI | TGGCCA | 1 cut(s) | 4339 |
| MslI | CAYNNNNRTG | 5 cut(s) | 122, 1323, 2165, 2577, 4214 |
| Msp20I | TGGCCA | 1 cut(s) | 4339 |
| MspA1I | CMGCKG | 4 cut(s) | 995, 1694, 3488, 4352 |
| MspCI | CTTAAG | 2 cut(s) | 3368, 4103 |
| MspI | CCGG | 5 cut(s) | 227, 260, 1772, 4232, 4269 |
| MunI | CAATTG | 1 cut(s) | 501 |
| Mva1269I | GAATGC | 3 cut(s) | 610, 2381, 4273 |
| MvnI | CGCG | 1 cut(s) | 3301 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 256, 296, 1078, 1209, 1218, 1256, 2288, 3072, 3749 |
| NciI | CCSGG | 4 cut(s) | 227, 261, 4233, 4270 |
| NcoI | CCATGG | 2 cut(s) | 1779, 3810 |
| NdeI | CATATG | 1 cut(s) | 3421 |
| NlaIV | GGNNCC | 9 cut(s) | 204, 1072, 1258, 1543, 1620, 1776, 3408, 3892, 4440 |
| NmeAIII | GCCGAG | 3 cut(s) | 1448, 1773, 1918 |
| NmuCI | GTSAC | 5 cut(s) | 851, 1532, 2909, 3052, 4045 |
| NsiI | ATGCAT | 2 cut(s) | 3658, 4658 |
| NspI | RCATGY | 7 cut(s) | 220, 548, 569, 1556, 2112, 3531, 4414 |
| NspV | TTCGAA | 1 cut(s) | 1749 |
| OliI | CACNNNNGTG | 1 cut(s) | 1323 |
| PaeI | GCATGC | 2 cut(s) | 220, 548 |
| PaeR7I | CTCGAG | 1 cut(s) | 1225 |
| PasI | CCCWGGG | 1 cut(s) | 4824 |
| PciI | ACATGT | 1 cut(s) | 4410 |
| PciSI | GCTCTTC | 3 cut(s) | 468, 1855, 4804 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1162, 4147 |
| PctI | GAATGC | 3 cut(s) | 610, 2381, 4273 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1670 |
| PfoI | TCCNGGA | 1 cut(s) | 2113 |
| Ple19I | CGATCG | 1 cut(s) | 1324 |
| PleI | GAGTC | 9 cut(s) | 76, 278, 344, 1817, 2318, 2786, 3124, 3575, 4594 |
| PmaCI | CACGTG | 1 cut(s) | 2234 |
| PmlI | CACGTG | 1 cut(s) | 2234 |
| PpsI | GAGTC | 9 cut(s) | 76, 278, 344, 1817, 2318, 2786, 3124, 3575, 4594 |
| Ppu21I | YACGTR | 2 cut(s) | 2034, 2234 |
| PpuMI | RGGWCCY | 1 cut(s) | 1835 |
| PscI | ACATGT | 1 cut(s) | 4410 |
| PshBI | ATTAAT | 1 cut(s) | 2750 |
| Psp124BI | GAGCTC | 1 cut(s) | 2614 |
| Psp5II | RGGWCCY | 1 cut(s) | 1835 |
| PspCI | CACGTG | 1 cut(s) | 2234 |
| PspFI | CCCAGC | 2 cut(s) | 1694, 4367 |
| PspN4I | GGNNCC | 9 cut(s) | 204, 1072, 1258, 1543, 1620, 1776, 3408, 3892, 4440 |
| PspOMI | GGGCCC | 1 cut(s) | 1070 |
| PspPPI | RGGWCCY | 1 cut(s) | 1835 |
| PspXI | VCTCGAGB | 1 cut(s) | 1225 |
| PstI | CTGCAG | 8 cut(s) | 1059, 1636, 2994, 3291, 4041, 4351, 4770, 4791 |
| PstNI | CAGNNNCTG | 5 cut(s) | 385, 1631, 1670, 1838, 4786 |
| PsuI | RGATCY | 8 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154, 4193 |
| PvuI | CGATCG | 1 cut(s) | 1324 |
| PvuII | CAGCTG | 3 cut(s) | 995, 1694, 4352 |
| RseI | CAYNNNNRTG | 5 cut(s) | 122, 1323, 2165, 2577, 4214 |
| SacI | GAGCTC | 1 cut(s) | 2614 |
| SalI | GTCGAC | 1 cut(s) | 1682 |
| SapI | GCTCTTC | 3 cut(s) | 468, 1855, 4804 |
| ScaI | AGTACT | 3 cut(s) | 603, 3151, 4005 |
| SchI | GAGTC | 9 cut(s) | 77, 278, 344, 1818, 2318, 2786, 3125, 3575, 4594 |
| SduI | GDGCHC | 7 cut(s) | 577, 1074, 1623, 2614, 3185, 3671, 3833 |
| SexAI | ACCWGGT | 2 cut(s) | 844, 4441 |
| Sfr274I | CTCGAG | 1 cut(s) | 1225 |
| SfuI | TTCGAA | 1 cut(s) | 1749 |
| SinI | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| SlaI | CTCGAG | 1 cut(s) | 1225 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 122, 1323, 2165, 2577, 4214 |
| SpeI | ACTAGT | 1 cut(s) | 4073 |
| SphI | GCATGC | 2 cut(s) | 220, 548 |
| SsiI | CCGC | 5 cut(s) | 1659, 3073, 3301, 3486, 4436 |
| SspI | AATATT | 1 cut(s) | 537 |
| SspMI | CTAG | 8 cut(s) | 726, 1268, 1992, 2643, 3509, 3662, 4074, 4305 |
| SstI | GAGCTC | 1 cut(s) | 2614 |
| StyI | CCWWGG | 4 cut(s) | 197, 1779, 3810, 4873 |
| TaiI | ACGT | 5 cut(s) | 774, 1168, 2036, 2236, 3932 |
| TaqI | TCGA | 7 cut(s) | 1226, 1366, 1683, 1749, 2624, 3468, 4141 |
| TaqII | GACCGA | 1 cut(s) | 3325 |
| TatI | WGTACW | 5 cut(s) | 601, 2224, 2755, 3149, 4003 |
| TauI | GCSGC | 3 cut(s) | 3075, 3304, 3488 |
| TseFI | GTSAC | 5 cut(s) | 851, 1532, 2909, 3052, 4045 |
| Tsp45I | GTSAC | 5 cut(s) | 851, 1532, 2909, 3052, 4045 |
| TspGWI | ACGGA | 2 cut(s) | 2208, 3883 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1670 |
| Vha464I | CTTAAG | 2 cut(s) | 3368, 4103 |
| VpaK11BI | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| VspI | ATTAAT | 1 cut(s) | 2750 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| XapI | RAATTY | 5 cut(s) | 506, 622, 1871, 2098, 2332 |
| XbaI | TCTAGA | 1 cut(s) | 1991 |
| XceI | RCATGY | 7 cut(s) | 220, 548, 569, 1556, 2112, 3531, 4414 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 589, 2171, 2885 |
| XhoI | CTCGAG | 1 cut(s) | 1225 |
| XmiI | GTMKAC | 4 cut(s) | 1297, 1683, 3776, 4723 |
| XspI | CTAG | 8 cut(s) | 726, 1268, 1992, 2643, 3509, 3662, 4074, 4305 |
| ZrmI | AGTACT | 3 cut(s) | 603, 3151, 4005 |
| Zsp2I | ATGCAT | 2 cut(s) | 3658, 4658 |