Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G55325 AT1G55325 AT1G55325 AT1G55325 |
| fragaria_vesca | FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 |
| malus_domestica | MD03G1004100.v1.1 |
| prunus_persica | Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 |
| pyrus_communis | pycom03g00420 |
| rosa_chinensis | RchiOBHm_Chr5g0083741 |
| rosa_laevigata | RLG00000037049 |
| rosa_multiflora | Rmu_sc0001287.1_g000006 |
| rosa_roxburghii | Rroxscaffold_1G00000840 |
| rosa_rugosa | Rorug05G0485500 Rorug05G0485600 Rorug05G0485700 |
| rosa_samantha | Rh5AG538600 Rh5BG564000 Rh5CG585900 Rh5DG572400 |
| rosa_wichuraiana | Rw5G050090 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 5221 |
| Acc36I | ACCTGC | 4 cut(s) | 2753, 3174, 4663, 5221 |
| AccB1I | GGYRCC | 3 cut(s) | 1421, 1654, 4202 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1550 |
| AccBSI | CCGCTC | 1 cut(s) | 1541 |
| AccI | GTMKAC | 3 cut(s) | 1177, 1563, 4606 |
| AccII | CGCG | 1 cut(s) | 3181 |
| AccIII | TCCGGA | 1 cut(s) | 5165 |
| AciI | CCGC | 7 cut(s) | 1539, 2953, 3181, 3366, 4319, 4822, 5638 |
| AcoI | YGGCCR | 1 cut(s) | 4220 |
| AcsI | RAATTY | 6 cut(s) | 383, 499, 1751, 1978, 2212, 5543 |
| AcuI | CTGAAG | 9 cut(s) | 423, 503, 745, 1716, 2408, 2588, 3626, 5033, 5255 |
| AcvI | CACGTG | 1 cut(s) | 2114 |
| AcyI | GRCGYC | 1 cut(s) | 2273 |
| AdeI | CACNNNGTG | 2 cut(s) | 347, 1046 |
| AflII | CTTAAG | 2 cut(s) | 3248, 3983 |
| AflIII | ACRYGT | 4 cut(s) | 1911, 2111, 4293, 5282 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 5616 |
| AhlI | ACTAGT | 1 cut(s) | 3953 |
| AjiI | CACGTC | 1 cut(s) | 649 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 690, 722, 812, 844, 4961, 4993 |
| AleI | CACNNNNGTG | 2 cut(s) | 1203, 5189 |
| Alw21I | GWGCWC | 5 cut(s) | 454, 2494, 5017, 5140, 5712 |
| Alw44I | GTGCAC | 1 cut(s) | 5013 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 262, 1511, 1550, 1718, 4669 |
| Ama87I | CYCGRG | 3 cut(s) | 1105, 2496, 3719 |
| Aor13HI | TCCGGA | 1 cut(s) | 5165 |
| ApaI | GGGCCC | 1 cut(s) | 951 |
| ApaLI | GTGCAC | 1 cut(s) | 5013 |
| ApoI | RAATTY | 6 cut(s) | 383, 499, 1751, 1978, 2212, 5543 |
| AseI | ATTAAT | 2 cut(s) | 2630, 4776 |
| AspLEI | GCGC | 4 cut(s) | 1242, 2464, 2481, 4527 |
| AsuC2I | CCSGG | 3 cut(s) | 104, 4116, 4153 |
| AsuII | TTCGAA | 1 cut(s) | 1629 |
| AvaI | CYCGRG | 3 cut(s) | 1105, 2496, 3719 |
| AvaII | GGWCC | 4 cut(s) | 54, 1715, 2690, 2755 |
| BaeGI | GKGCMC | 3 cut(s) | 951, 3713, 5017 |
| BalI | TGGCCA | 1 cut(s) | 4222 |
| BamHI | GGATCC | 2 cut(s) | 79, 3770 |
| BanI | GGYRCC | 3 cut(s) | 1421, 1654, 4202 |
| BanII | GRGCYC | 8 cut(s) | 951, 1503, 2494, 3065, 3551, 5140, 5162, 5712 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 2068, 2100, 2767, 2799, 4849, 4881 |
| BauI | CACGAG | 1 cut(s) | 4029 |
| BbrPI | CACGTG | 1 cut(s) | 2114 |
| BbsI | GAAGAC | 4 cut(s) | 1967, 2298, 3772, 4095 |
| Bbv12I | GWGCWC | 5 cut(s) | 454, 2494, 5017, 5140, 5712 |
| BbvCI | CCTCAGC | 1 cut(s) | 873 |
| BceAI | ACGGC | 2 cut(s) | 2306, 2904 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1415, 1449, 3710, 3744 |
| BciVI | GTATCC | 2 cut(s) | 1300, 4432 |
| BclI | TGATCA | 4 cut(s) | 814, 1594, 1756, 5452 |
| BcnI | CCSGG | 3 cut(s) | 104, 4116, 4153 |
| BcuI | ACTAGT | 1 cut(s) | 3953 |
| BfoI | RGCGCY | 3 cut(s) | 1243, 2482, 4528 |
| BfrI | CTTAAG | 2 cut(s) | 3248, 3983 |
| BfuAI | ACCTGC | 4 cut(s) | 2753, 3174, 4663, 5221 |
| BfuI | GTATCC | 2 cut(s) | 1300, 4432 |
| BglII | AGATCT | 1 cut(s) | 3803 |
| BmcAI | AGTACT | 3 cut(s) | 480, 3031, 3885 |
| Bme18I | GGWCC | 4 cut(s) | 54, 1715, 2690, 2755 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 5616 |
| BmeT110I | CYCGRG | 3 cut(s) | 1105, 2496, 3719 |
| BmgBI | CACGTC | 1 cut(s) | 649 |
| BmrI | ACTGGG | 1 cut(s) | 5522 |
| BmuI | ACTGGG | 1 cut(s) | 5522 |
| BpiI | GAAGAC | 4 cut(s) | 1967, 2298, 3772, 4095 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 898, 930, 3446, 3478 |
| BpmI | CTGGAG | 5 cut(s) | 225, 1527, 4646, 5154, 5651 |
| Bpu10I | CCTNAGC | 1 cut(s) | 873 |
| Bpu14I | TTCGAA | 1 cut(s) | 1629 |
| BpuEI | CTTGAG | 9 cut(s) | 153, 639, 1257, 2497, 3314, 3492, 4440, 5020, 5124 |
| BpuMI | CCSGG | 3 cut(s) | 104, 4116, 4153 |
| Bsa29I | ATCGAT | 1 cut(s) | 3348 |
| BsaAI | YACGTR | 3 cut(s) | 1311, 1914, 2114 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2451, 4831 |
| BsaHI | GRCGYC | 1 cut(s) | 2273 |
| BsaI | GGTCTC | 1 cut(s) | 2506 |
| BsaWI | WCCGGW | 1 cut(s) | 5165 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1777, 1807 |
| Bse118I | RCCGGY | 1 cut(s) | 1139 |
| Bse3DI | GCAATG | 5 cut(s) | 2802, 3209, 3519, 3538, 3809 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2451, 4831 |
| BseAI | TCCGGA | 1 cut(s) | 5165 |
| BseCI | ATCGAT | 1 cut(s) | 3348 |
| BseJI | GATNNNNATC | 2 cut(s) | 2451, 4831 |
| BseMI | GCAATG | 5 cut(s) | 2802, 3209, 3519, 3538, 3809 |
| BseRI | GAGGAG | 3 cut(s) | 522, 2484, 5695 |
| BseSI | GKGCMC | 3 cut(s) | 951, 3713, 5017 |
| BseYI | CCCAGC | 2 cut(s) | 889, 4250 |
| BsgI | GTGCAG | 2 cut(s) | 1227, 3254 |
| Bsh1236I | CGCG | 1 cut(s) | 3181 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1204 |
| BshNI | GGYRCC | 3 cut(s) | 1421, 1654, 4202 |
| BshVI | ATCGAT | 1 cut(s) | 3348 |
| BsiEI | CGRYCG | 1 cut(s) | 1204 |
| BsiHKAI | GWGCWC | 5 cut(s) | 454, 2494, 5017, 5140, 5712 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1105, 2496, 3719 |
| BsiSI | CCGG | 6 cut(s) | 104, 1140, 1652, 4115, 4152, 5166 |
| BslFI | GGGAC | 7 cut(s) | 239, 872, 2209, 3602, 4635, 4724, 4995 |
| BsmBI | CGTCTC | 1 cut(s) | 655 |
| BsmFI | GGGAC | 7 cut(s) | 239, 872, 2209, 3602, 4635, 4724, 4995 |
| BsmI | GAATGC | 3 cut(s) | 487, 2261, 4156 |
| Bso31I | GGTCTC | 1 cut(s) | 2506 |
| BsoBI | CYCGRG | 3 cut(s) | 1105, 2496, 3719 |
| Bsp119I | TTCGAA | 1 cut(s) | 1629 |
| Bsp120I | GGGCCC | 1 cut(s) | 947 |
| Bsp13I | TCCGGA | 1 cut(s) | 5165 |
| Bsp1407I | TGTACA | 1 cut(s) | 2635 |
| Bsp19I | CCATGG | 2 cut(s) | 1659, 3690 |
| BspACI | CCGC | 7 cut(s) | 1539, 2953, 3181, 3366, 4319, 4822, 5638 |
| BspDI | ATCGAT | 1 cut(s) | 3348 |
| BspEI | TCCGGA | 1 cut(s) | 5165 |
| BspFNI | CGCG | 1 cut(s) | 3181 |
| BspHI | TCATGA | 1 cut(s) | 5490 |
| BspMAI | CTGCAG | 8 cut(s) | 936, 1516, 2874, 3171, 3921, 4234, 4653, 4674 |
| BspMI | ACCTGC | 4 cut(s) | 2753, 3174, 4663, 5221 |
| BspQI | GCTCTTC | 3 cut(s) | 345, 1735, 4687 |
| BspT104I | TTCGAA | 1 cut(s) | 1629 |
| BspT107I | GGYRCC | 3 cut(s) | 1421, 1654, 4202 |
| BspTI | CTTAAG | 2 cut(s) | 3248, 3983 |
| BspTNI | GGTCTC | 1 cut(s) | 2506 |
| BsrBI | CCGCTC | 1 cut(s) | 1541 |
| BsrDI | GCAATG | 5 cut(s) | 2802, 3209, 3519, 3538, 3809 |
| BsrFI | RCCGGY | 1 cut(s) | 1139 |
| BsrGI | TGTACA | 1 cut(s) | 2635 |
| BssAI | RCCGGY | 1 cut(s) | 1139 |
| BssNI | GRCGYC | 1 cut(s) | 2273 |
| BssSI | CACGAG | 1 cut(s) | 4029 |
| BssT1I | CCWWGG | 3 cut(s) | 74, 1659, 3690 |
| Bst2BI | CACGAG | 1 cut(s) | 4029 |
| Bst6I | CTCTTC | 9 cut(s) | 228, 345, 399, 802, 1659, 1735, 2384, 4687, 5274 |
| BstACI | GRCGYC | 1 cut(s) | 2273 |
| BstAFI | CTTAAG | 2 cut(s) | 3248, 3983 |
| BstAUI | TGTACA | 1 cut(s) | 2635 |
| BstBAI | YACGTR | 3 cut(s) | 1311, 1914, 2114 |
| BstBI | TTCGAA | 1 cut(s) | 1629 |
| BstDSI | CCRYGG | 2 cut(s) | 1659, 3690 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 254, 801 |
| BstFNI | CGCG | 1 cut(s) | 3181 |
| BstH2I | RGCGCY | 3 cut(s) | 1243, 2482, 4528 |
| BstHHI | GCGC | 4 cut(s) | 1242, 2464, 2481, 4527 |
| BstMCI | CGRYCG | 1 cut(s) | 1204 |
| BstNSI | RCATGY | 9 cut(s) | 97, 425, 446, 1436, 1992, 3411, 4297, 5248, 5286 |
| BstSLI | GKGCMC | 3 cut(s) | 951, 3713, 5017 |
| BstSNI | TACGTA | 1 cut(s) | 1311 |
| BstUI | CGCG | 1 cut(s) | 3181 |
| BstV2I | GAAGAC | 4 cut(s) | 1967, 2298, 3772, 4095 |
| BstX2I | RGATCY | 8 cut(s) | 79, 824, 1000, 1382, 3770, 3803, 4034, 4076 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 4258 |
| BstYI | RGATCY | 8 cut(s) | 79, 824, 1000, 1382, 3770, 3803, 4034, 4076 |
| Bsu15I | ATCGAT | 1 cut(s) | 3348 |
| BsuI | GTATCC | 2 cut(s) | 1300, 4432 |
| BsuTUI | ATCGAT | 1 cut(s) | 3348 |
| BtgI | CCRYGG | 2 cut(s) | 1659, 3690 |
| BtgZI | GCGATG | 1 cut(s) | 1569 |
| BtrI | CACGTC | 1 cut(s) | 649 |
| BtsI | GCAGTG | 6 cut(s) | 183, 885, 2059, 2726, 3858, 5289 |
| BveI | ACCTGC | 4 cut(s) | 2753, 3174, 4663, 5221 |
| CaiI | CAGNNNCTG | 5 cut(s) | 262, 1511, 1550, 1718, 4669 |
| CciI | TCATGA | 1 cut(s) | 5490 |
| CfoI | GCGC | 4 cut(s) | 1242, 2464, 2481, 4527 |
| Cfr10I | RCCGGY | 1 cut(s) | 1139 |
| ClaI | ATCGAT | 1 cut(s) | 3348 |
| CseI | GACGC | 3 cut(s) | 1166, 2262, 4193 |
| CsiI | ACCWGGT | 1 cut(s) | 4324 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2702, 2737 |
| DraIII | CACNNNGTG | 2 cut(s) | 347, 1046 |
| DriI | GACNNNNNGTC | 1 cut(s) | 5616 |
| EaeI | YGGCCR | 1 cut(s) | 4220 |
| Eam1104I | CTCTTC | 9 cut(s) | 228, 345, 399, 802, 1659, 1735, 2384, 4687, 5274 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 5616 |
| EarI | CTCTTC | 9 cut(s) | 228, 345, 399, 802, 1659, 1735, 2384, 4687, 5274 |
| EciI | GGCGGA | 1 cut(s) | 5653 |
| Ecl136II | GAGCTC | 3 cut(s) | 2492, 5138, 5710 |
| Eco105I | TACGTA | 1 cut(s) | 1311 |
| Eco130I | CCWWGG | 3 cut(s) | 74, 1659, 3690 |
| Eco24I | GRGCYC | 8 cut(s) | 951, 1503, 2494, 3065, 3551, 5140, 5162, 5712 |
| Eco31I | GGTCTC | 1 cut(s) | 2506 |
| Eco32I | GATATC | 2 cut(s) | 216, 5171 |
| Eco47I | GGWCC | 4 cut(s) | 54, 1715, 2690, 2755 |
| Eco53kI | GAGCTC | 3 cut(s) | 2492, 5138, 5710 |
| Eco57I | CTGAAG | 9 cut(s) | 423, 503, 745, 1716, 2408, 2588, 3626, 5033, 5255 |
| Eco72I | CACGTG | 1 cut(s) | 2114 |
| Eco88I | CYCGRG | 3 cut(s) | 1105, 2496, 3719 |
| EcoICRI | GAGCTC | 3 cut(s) | 2492, 5138, 5710 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 254, 801 |
| EcoO109I | RGGNCCY | 4 cut(s) | 735, 947, 948, 1715 |
| EcoRI | GAATTC | 1 cut(s) | 1978 |
| EcoRV | GATATC | 2 cut(s) | 216, 5171 |
| EcoT14I | CCWWGG | 3 cut(s) | 74, 1659, 3690 |
| EcoT22I | ATGCAT | 2 cut(s) | 3538, 4541 |
| EcoT38I | GRGCYC | 8 cut(s) | 951, 1503, 2494, 3065, 3551, 5140, 5162, 5712 |
| ErhI | CCWWGG | 3 cut(s) | 74, 1659, 3690 |
| Esp3I | CGTCTC | 1 cut(s) | 655 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 690, 722, 1282, 1314, 2876, 2908 |
| FaqI | GGGAC | 7 cut(s) | 239, 872, 2209, 3602, 4635, 4724, 4995 |
| FauNDI | CATATG | 1 cut(s) | 3301 |
| FbaI | TGATCA | 4 cut(s) | 814, 1594, 1756, 5452 |
| FblI | GTMKAC | 3 cut(s) | 1177, 1563, 4606 |
| FriOI | GRGCYC | 8 cut(s) | 951, 1503, 2494, 3065, 3551, 5140, 5162, 5712 |
| GlaI | GCGC | 4 cut(s) | 1241, 2463, 2480, 4526 |
| GsaI | CCCAGC | 2 cut(s) | 893, 4254 |
| GsuI | CTGGAG | 5 cut(s) | 225, 1527, 4646, 5154, 5651 |
| HaeII | RGCGCY | 3 cut(s) | 1243, 2482, 4528 |
| HapII | CCGG | 6 cut(s) | 104, 1140, 1652, 4115, 4152, 5166 |
| HgaI | GACGC | 3 cut(s) | 1166, 2262, 4193 |
| HhaI | GCGC | 4 cut(s) | 1242, 2464, 2481, 4527 |
| Hin1I | GRCGYC | 1 cut(s) | 2273 |
| Hin6I | GCGC | 4 cut(s) | 1240, 2462, 2479, 4525 |
| HinP1I | GCGC | 4 cut(s) | 1240, 2462, 2479, 4525 |
| HincII | GTYRAC | 4 cut(s) | 1342, 1564, 2820, 3460 |
| HindII | GTYRAC | 4 cut(s) | 1342, 1564, 2820, 3460 |
| HindIII | AAGCTT | 1 cut(s) | 3630 |
| HpaI | GTTAAC | 1 cut(s) | 2820 |
| HpaII | CCGG | 6 cut(s) | 104, 1140, 1652, 4115, 4152, 5166 |
| Hpy99I | CGWCG | 1 cut(s) | 4023 |
| HpyCH4IV | ACGT | 5 cut(s) | 648, 1045, 1310, 1913, 2113 |
| HpySE526I | ACGT | 5 cut(s) | 648, 1045, 1310, 1913, 2113 |
| Hsp92I | GRCGYC | 1 cut(s) | 2273 |
| HspAI | GCGC | 4 cut(s) | 1240, 2462, 2479, 4525 |
| Kpn2I | TCCGGA | 1 cut(s) | 5165 |
| Ksp22I | TGATCA | 4 cut(s) | 814, 1594, 1756, 5452 |
| KspAI | GTTAAC | 1 cut(s) | 2820 |
| LguI | GCTCTTC | 3 cut(s) | 345, 1735, 4687 |
| MabI | ACCWGGT | 1 cut(s) | 4324 |
| MaeII | ACGT | 5 cut(s) | 648, 1045, 1310, 1913, 2113 |
| MbiI | CCGCTC | 1 cut(s) | 1541 |
| MfeI | CAATTG | 4 cut(s) | 378, 4817, 5079, 5085 |
| MflI | RGATCY | 8 cut(s) | 79, 824, 1000, 1382, 3770, 3803, 4034, 4076 |
| MlsI | TGGCCA | 1 cut(s) | 4222 |
| MluNI | TGGCCA | 1 cut(s) | 4222 |
| MmeI | TCCRAC | 4 cut(s) | 1672, 2895, 3058, 5285 |
| Mox20I | TGGCCA | 1 cut(s) | 4222 |
| Mph1103I | ATGCAT | 2 cut(s) | 3538, 4541 |
| MroI | TCCGGA | 1 cut(s) | 5165 |
| MscI | TGGCCA | 1 cut(s) | 4222 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1203, 2045, 2457, 4097, 5189 |
| Msp20I | TGGCCA | 1 cut(s) | 4222 |
| MspA1I | CMGCKG | 4 cut(s) | 872, 3368, 4235, 5275 |
| MspCI | CTTAAG | 2 cut(s) | 3248, 3983 |
| MspI | CCGG | 6 cut(s) | 104, 1140, 1652, 4115, 4152, 5166 |
| MunI | CAATTG | 4 cut(s) | 378, 4817, 5079, 5085 |
| Mva1269I | GAATGC | 3 cut(s) | 487, 2261, 4156 |
| MvnI | CGCG | 1 cut(s) | 3181 |
| NciI | CCSGG | 3 cut(s) | 104, 4116, 4153 |
| NcoI | CCATGG | 2 cut(s) | 1659, 3690 |
| NdeI | CATATG | 1 cut(s) | 3301 |
| NmeAIII | GCCGAG | 3 cut(s) | 1328, 1653, 1798 |
| NmuCI | GTSAC | 8 cut(s) | 728, 1412, 2789, 2932, 3925, 4917, 5074, 5235 |
| NsiI | ATGCAT | 2 cut(s) | 3538, 4541 |
| NspI | RCATGY | 9 cut(s) | 97, 425, 446, 1436, 1992, 3411, 4297, 5248, 5286 |
| NspV | TTCGAA | 1 cut(s) | 1629 |
| OliI | CACNNNNGTG | 2 cut(s) | 1203, 5189 |
| PaeI | GCATGC | 3 cut(s) | 97, 425, 5248 |
| PaeR7I | CTCGAG | 1 cut(s) | 1105 |
| PagI | TCATGA | 1 cut(s) | 5490 |
| PaqCI | CACCTGC | 1 cut(s) | 5221 |
| PasI | CCCWGGG | 1 cut(s) | 4707 |
| PciI | ACATGT | 2 cut(s) | 4293, 5282 |
| PciSI | GCTCTTC | 3 cut(s) | 345, 1735, 4687 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1042, 4027 |
| PctI | GAATGC | 3 cut(s) | 487, 2261, 4156 |
| PflFI | GACNNNGTC | 1 cut(s) | 1261 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1550 |
| PfoI | TCCNGGA | 2 cut(s) | 1993, 5131 |
| Ple19I | CGATCG | 1 cut(s) | 1204 |
| PmaCI | CACGTG | 1 cut(s) | 2114 |
| PmlI | CACGTG | 1 cut(s) | 2114 |
| Ppu21I | YACGTR | 3 cut(s) | 1311, 1914, 2114 |
| PpuMI | RGGWCCY | 1 cut(s) | 1715 |
| PscI | ACATGT | 2 cut(s) | 4293, 5282 |
| PshBI | ATTAAT | 2 cut(s) | 2630, 4776 |
| Psp124BI | GAGCTC | 3 cut(s) | 2494, 5140, 5712 |
| Psp5II | RGGWCCY | 1 cut(s) | 1715 |
| PspCI | CACGTG | 1 cut(s) | 2114 |
| PspFI | CCCAGC | 2 cut(s) | 889, 4250 |
| PspOMI | GGGCCC | 1 cut(s) | 947 |
| PspPPI | RGGWCCY | 1 cut(s) | 1715 |
| PspXI | VCTCGAGB | 1 cut(s) | 1105 |
| PstI | CTGCAG | 8 cut(s) | 936, 1516, 2874, 3171, 3921, 4234, 4653, 4674 |
| PstNI | CAGNNNCTG | 5 cut(s) | 262, 1511, 1550, 1718, 4669 |
| PsuI | RGATCY | 8 cut(s) | 79, 824, 1000, 1382, 3770, 3803, 4034, 4076 |
| PsyI | GACNNNGTC | 1 cut(s) | 1261 |
| PvuI | CGATCG | 1 cut(s) | 1204 |
| PvuII | CAGCTG | 3 cut(s) | 872, 4235, 5275 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1203, 2045, 2457, 4097, 5189 |
| SacI | GAGCTC | 3 cut(s) | 2494, 5140, 5712 |
| SalI | GTCGAC | 1 cut(s) | 1562 |
| SapI | GCTCTTC | 3 cut(s) | 345, 1735, 4687 |
| ScaI | AGTACT | 3 cut(s) | 480, 3031, 3885 |
| SexAI | ACCWGGT | 1 cut(s) | 4324 |
| Sfr274I | CTCGAG | 1 cut(s) | 1105 |
| SfuI | TTCGAA | 1 cut(s) | 1629 |
| SinI | GGWCC | 4 cut(s) | 54, 1715, 2690, 2755 |
| SlaI | CTCGAG | 1 cut(s) | 1105 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1203, 2045, 2457, 4097, 5189 |
| SnaBI | TACGTA | 1 cut(s) | 1311 |
| SpeI | ACTAGT | 1 cut(s) | 3953 |
| SphI | GCATGC | 3 cut(s) | 97, 425, 5248 |
| SsiI | CCGC | 7 cut(s) | 1539, 2953, 3181, 3366, 4319, 4822, 5638 |
| SspI | AATATT | 1 cut(s) | 414 |
| SstI | GAGCTC | 3 cut(s) | 2494, 5140, 5712 |
| StyI | CCWWGG | 3 cut(s) | 74, 1659, 3690 |
| TaiI | ACGT | 5 cut(s) | 651, 1048, 1313, 1916, 2116 |
| TaqI | TCGA | 7 cut(s) | 1106, 1246, 1563, 1629, 2504, 3348, 4021 |
| TaqII | GACCGA | 1 cut(s) | 3205 |
| TatI | WGTACW | 5 cut(s) | 478, 2104, 2635, 3029, 3883 |
| TauI | GCSGC | 4 cut(s) | 2955, 3184, 3368, 4825 |
| TseFI | GTSAC | 8 cut(s) | 728, 1412, 2789, 2932, 3925, 4917, 5074, 5235 |
| Tsp45I | GTSAC | 8 cut(s) | 728, 1412, 2789, 2932, 3925, 4917, 5074, 5235 |
| TspGWI | ACGGA | 1 cut(s) | 3763 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1261 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1550 |
| Vha464I | CTTAAG | 2 cut(s) | 3248, 3983 |
| VneI | GTGCAC | 1 cut(s) | 5013 |
| VpaK11BI | GGWCC | 4 cut(s) | 54, 1715, 2690, 2755 |
| VspI | ATTAAT | 2 cut(s) | 2630, 4776 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 254, 801 |
| XapI | RAATTY | 6 cut(s) | 383, 499, 1751, 1978, 2212, 5543 |
| XbaI | TCTAGA | 1 cut(s) | 1871 |
| XceI | RCATGY | 9 cut(s) | 97, 425, 446, 1436, 1992, 3411, 4297, 5248, 5286 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 466, 2051, 2765 |
| XhoI | CTCGAG | 1 cut(s) | 1105 |
| XmiI | GTMKAC | 3 cut(s) | 1177, 1563, 4606 |
| ZrmI | AGTACT | 3 cut(s) | 480, 3031, 3885 |
| Zsp2I | ATGCAT | 2 cut(s) | 3538, 4541 |