Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G55325 AT1G55325 AT1G55325 AT1G55325 |
| fragaria_vesca | FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 |
| malus_domestica | MD03G1004100.v1.1 |
| prunus_persica | Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 |
| pyrus_communis | pycom03g00420 |
| rosa_chinensis | RchiOBHm_Chr5g0083741 |
| rosa_laevigata | RLG00000037049 |
| rosa_multiflora | Rmu_sc0001287.1_g000006 |
| rosa_roxburghii | Rroxscaffold_1G00000840 |
| rosa_rugosa | Rorug05G0485500 Rorug05G0485600 Rorug05G0485700 |
| rosa_samantha | Rh5AG538600 Rh5BG564000 Rh5CG585900 Rh5DG572400 |
| rosa_wichuraiana | Rw5G050090 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 3 cut(s) | 2876, 3297, 4786 |
| AccB1I | GGYRCC | 3 cut(s) | 1544, 1777, 4325 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1673 |
| AccBSI | CCGCTC | 1 cut(s) | 1664 |
| AccI | GTMKAC | 4 cut(s) | 1300, 1686, 3779, 4729 |
| AccII | CGCG | 2 cut(s) | 3304, 4464 |
| AccIII | TCCGGA | 1 cut(s) | 5288 |
| AciI | CCGC | 9 cut(s) | 1662, 2679, 3076, 3304, 3489, 4442, 4464, 4945, 5764 |
| AcoI | YGGCCR | 1 cut(s) | 4343 |
| AcsI | RAATTY | 6 cut(s) | 506, 622, 1874, 2101, 2335, 5669 |
| AcvI | CACGTG | 1 cut(s) | 2237 |
| AcyI | GRCGYC | 1 cut(s) | 2396 |
| AdeI | CACNNNGTG | 2 cut(s) | 470, 1169 |
| AflII | CTTAAG | 2 cut(s) | 3371, 4106 |
| AflIII | ACRYGT | 4 cut(s) | 2034, 2234, 4416, 5405 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 5742 |
| AhlI | ACTAGT | 1 cut(s) | 4076 |
| AjiI | CACGTC | 1 cut(s) | 772 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 813, 845, 935, 967, 5084, 5116 |
| AleI | CACNNNNGTG | 2 cut(s) | 1326, 5312 |
| Alw21I | GWGCWC | 5 cut(s) | 577, 2617, 5140, 5263, 5838 |
| Alw44I | GTGCAC | 1 cut(s) | 5136 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 385, 1634, 1673, 1841, 4792 |
| Ama87I | CYCGRG | 3 cut(s) | 1228, 2619, 3842 |
| Aor13HI | TCCGGA | 1 cut(s) | 5288 |
| ApaI | GGGCCC | 1 cut(s) | 1074 |
| ApaLI | GTGCAC | 1 cut(s) | 5136 |
| ApoI | RAATTY | 6 cut(s) | 506, 622, 1874, 2101, 2335, 5669 |
| AseI | ATTAAT | 2 cut(s) | 2753, 4899 |
| AspLEI | GCGC | 4 cut(s) | 1365, 2587, 2604, 4650 |
| AsuC2I | CCSGG | 2 cut(s) | 227, 4276 |
| AsuII | TTCGAA | 1 cut(s) | 1752 |
| AvaI | CYCGRG | 3 cut(s) | 1228, 2619, 3842 |
| AvaII | GGWCC | 5 cut(s) | 6, 177, 1838, 2813, 2878 |
| BaeGI | GKGCMC | 3 cut(s) | 1074, 3836, 5140 |
| BalI | TGGCCA | 1 cut(s) | 4345 |
| BamHI | GGATCC | 2 cut(s) | 202, 3893 |
| BanI | GGYRCC | 3 cut(s) | 1544, 1777, 4325 |
| BanII | GRGCYC | 7 cut(s) | 1074, 1626, 2617, 3188, 3674, 5263, 5838 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 2191, 2223, 2890, 2922, 4972, 5004 |
| BauI | CACGAG | 2 cut(s) | 65, 4152 |
| BbrPI | CACGTG | 1 cut(s) | 2237 |
| BbsI | GAAGAC | 4 cut(s) | 2090, 2421, 3895, 4218 |
| Bbv12I | GWGCWC | 5 cut(s) | 577, 2617, 5140, 5263, 5838 |
| BbvCI | CCTCAGC | 1 cut(s) | 996 |
| BceAI | ACGGC | 2 cut(s) | 2429, 3027 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1538, 1572, 3833, 3867 |
| BciVI | GTATCC | 3 cut(s) | 1423, 2403, 4555 |
| BclI | TGATCA | 5 cut(s) | 78, 937, 1717, 1879, 5575 |
| BcnI | CCSGG | 2 cut(s) | 227, 4276 |
| BcuI | ACTAGT | 1 cut(s) | 4076 |
| BfoI | RGCGCY | 3 cut(s) | 1366, 2605, 4651 |
| BfrI | CTTAAG | 2 cut(s) | 3371, 4106 |
| BfuAI | ACCTGC | 3 cut(s) | 2876, 3297, 4786 |
| BfuI | GTATCC | 3 cut(s) | 1423, 2403, 4555 |
| BglII | AGATCT | 1 cut(s) | 3926 |
| BmcAI | AGTACT | 3 cut(s) | 603, 3154, 4008 |
| Bme18I | GGWCC | 5 cut(s) | 6, 177, 1838, 2813, 2878 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 5742 |
| BmeT110I | CYCGRG | 3 cut(s) | 1228, 2619, 3842 |
| BmgBI | CACGTC | 1 cut(s) | 772 |
| BmrI | ACTGGG | 2 cut(s) | 4023, 5645 |
| BmuI | ACTGGG | 2 cut(s) | 4023, 5645 |
| BpiI | GAAGAC | 4 cut(s) | 2090, 2421, 3895, 4218 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1021, 1053, 3569, 3601 |
| BpmI | CTGGAG | 6 cut(s) | 348, 1650, 4221, 4769, 5277, 5777 |
| Bpu10I | CCTNAGC | 1 cut(s) | 996 |
| Bpu14I | TTCGAA | 1 cut(s) | 1752 |
| BpuEI | CTTGAG | 9 cut(s) | 276, 762, 1380, 2620, 3437, 3615, 4563, 5143, 5247 |
| BpuMI | CCSGG | 2 cut(s) | 227, 4276 |
| Bsa29I | ATCGAT | 1 cut(s) | 3471 |
| BsaAI | YACGTR | 2 cut(s) | 2037, 2237 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2574, 4954 |
| BsaHI | GRCGYC | 1 cut(s) | 2396 |
| BsaI | GGTCTC | 1 cut(s) | 2629 |
| BsaWI | WCCGGW | 1 cut(s) | 5288 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1900, 1930 |
| Bse3DI | GCAATG | 4 cut(s) | 2925, 3332, 3642, 3661 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2574, 4954 |
| BseAI | TCCGGA | 1 cut(s) | 5288 |
| BseCI | ATCGAT | 1 cut(s) | 3471 |
| BseJI | GATNNNNATC | 2 cut(s) | 2574, 4954 |
| BseMI | GCAATG | 4 cut(s) | 2925, 3332, 3642, 3661 |
| BseRI | GAGGAG | 3 cut(s) | 645, 2607, 5821 |
| BseSI | GKGCMC | 3 cut(s) | 1074, 3836, 5140 |
| BseYI | CCCAGC | 2 cut(s) | 1697, 4373 |
| BsgI | GTGCAG | 2 cut(s) | 1350, 3377 |
| Bsh1236I | CGCG | 2 cut(s) | 3304, 4464 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1327 |
| BshNI | GGYRCC | 3 cut(s) | 1544, 1777, 4325 |
| BshVI | ATCGAT | 1 cut(s) | 3471 |
| BsiEI | CGRYCG | 1 cut(s) | 1327 |
| BsiHKAI | GWGCWC | 5 cut(s) | 577, 2617, 5140, 5263, 5838 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1228, 2619, 3842 |
| BsiSI | CCGG | 4 cut(s) | 227, 1775, 4275, 5289 |
| BslFI | GGGAC | 7 cut(s) | 362, 995, 2332, 3725, 4758, 4847, 5118 |
| BsmBI | CGTCTC | 1 cut(s) | 778 |
| BsmFI | GGGAC | 7 cut(s) | 362, 995, 2332, 3725, 4758, 4847, 5118 |
| BsmI | GAATGC | 4 cut(s) | 610, 2384, 2681, 4279 |
| Bso31I | GGTCTC | 1 cut(s) | 2629 |
| BsoBI | CYCGRG | 3 cut(s) | 1228, 2619, 3842 |
| Bsp119I | TTCGAA | 1 cut(s) | 1752 |
| Bsp120I | GGGCCC | 1 cut(s) | 1070 |
| Bsp13I | TCCGGA | 1 cut(s) | 5288 |
| Bsp1407I | TGTACA | 1 cut(s) | 2758 |
| Bsp19I | CCATGG | 2 cut(s) | 1782, 3813 |
| BspACI | CCGC | 9 cut(s) | 1662, 2679, 3076, 3304, 3489, 4442, 4464, 4945, 5764 |
| BspDI | ATCGAT | 1 cut(s) | 3471 |
| BspEI | TCCGGA | 1 cut(s) | 5288 |
| BspFNI | CGCG | 2 cut(s) | 3304, 4464 |
| BspHI | TCATGA | 1 cut(s) | 5613 |
| BspMAI | CTGCAG | 8 cut(s) | 1059, 1639, 2997, 3294, 4044, 4357, 4776, 4797 |
| BspMI | ACCTGC | 3 cut(s) | 2876, 3297, 4786 |
| BspQI | GCTCTTC | 3 cut(s) | 468, 1858, 4810 |
| BspT104I | TTCGAA | 1 cut(s) | 1752 |
| BspT107I | GGYRCC | 3 cut(s) | 1544, 1777, 4325 |
| BspTI | CTTAAG | 2 cut(s) | 3371, 4106 |
| BspTNI | GGTCTC | 1 cut(s) | 2629 |
| BsrBI | CCGCTC | 1 cut(s) | 1664 |
| BsrDI | GCAATG | 4 cut(s) | 2925, 3332, 3642, 3661 |
| BsrGI | TGTACA | 1 cut(s) | 2758 |
| BssNI | GRCGYC | 1 cut(s) | 2396 |
| BssSI | CACGAG | 2 cut(s) | 65, 4152 |
| BssT1I | CCWWGG | 3 cut(s) | 197, 1782, 3813 |
| Bst2BI | CACGAG | 2 cut(s) | 65, 4152 |
| Bst6I | CTCTTC | 9 cut(s) | 351, 468, 522, 925, 1782, 1858, 2507, 4810, 5397 |
| BstACI | GRCGYC | 1 cut(s) | 2396 |
| BstAFI | CTTAAG | 2 cut(s) | 3371, 4106 |
| BstAUI | TGTACA | 1 cut(s) | 2758 |
| BstBAI | YACGTR | 2 cut(s) | 2037, 2237 |
| BstBI | TTCGAA | 1 cut(s) | 1752 |
| BstDSI | CCRYGG | 2 cut(s) | 1782, 3813 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| BstFNI | CGCG | 2 cut(s) | 3304, 4464 |
| BstH2I | RGCGCY | 3 cut(s) | 1366, 2605, 4651 |
| BstHHI | GCGC | 4 cut(s) | 1365, 2587, 2604, 4650 |
| BstMCI | CGRYCG | 1 cut(s) | 1327 |
| BstNSI | RCATGY | 9 cut(s) | 220, 548, 569, 1559, 2115, 3534, 4420, 5371, 5409 |
| BstSLI | GKGCMC | 3 cut(s) | 1074, 3836, 5140 |
| BstUI | CGCG | 2 cut(s) | 3304, 4464 |
| BstV2I | GAAGAC | 4 cut(s) | 2090, 2421, 3895, 4218 |
| BstX2I | RGATCY | 8 cut(s) | 202, 947, 1123, 1505, 3893, 3926, 4157, 4199 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 124, 4245, 4381 |
| BstYI | RGATCY | 8 cut(s) | 202, 947, 1123, 1505, 3893, 3926, 4157, 4199 |
| Bsu15I | ATCGAT | 1 cut(s) | 3471 |
| BsuI | GTATCC | 3 cut(s) | 1423, 2403, 4555 |
| BsuTUI | ATCGAT | 1 cut(s) | 3471 |
| BtgI | CCRYGG | 2 cut(s) | 1782, 3813 |
| BtgZI | GCGATG | 1 cut(s) | 1692 |
| BtrI | CACGTC | 1 cut(s) | 772 |
| BtsI | GCAGTG | 7 cut(s) | 106, 306, 1008, 2182, 2849, 3981, 5412 |
| BveI | ACCTGC | 3 cut(s) | 2876, 3297, 4786 |
| CaiI | CAGNNNCTG | 5 cut(s) | 385, 1634, 1673, 1841, 4792 |
| CciI | TCATGA | 1 cut(s) | 5613 |
| CfoI | GCGC | 4 cut(s) | 1365, 2587, 2604, 4650 |
| ClaI | ATCGAT | 1 cut(s) | 3471 |
| CseI | GACGC | 3 cut(s) | 1289, 2385, 4316 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2825, 2860 |
| DraI | TTTAAA | 1 cut(s) | 34 |
| DraIII | CACNNNGTG | 2 cut(s) | 470, 1169 |
| DriI | GACNNNNNGTC | 1 cut(s) | 5742 |
| EaeI | YGGCCR | 1 cut(s) | 4343 |
| Eam1104I | CTCTTC | 9 cut(s) | 351, 468, 522, 925, 1782, 1858, 2507, 4810, 5397 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 5742 |
| EarI | CTCTTC | 9 cut(s) | 351, 468, 522, 925, 1782, 1858, 2507, 4810, 5397 |
| EciI | GGCGGA | 1 cut(s) | 5779 |
| Ecl136II | GAGCTC | 3 cut(s) | 2615, 5261, 5836 |
| Eco130I | CCWWGG | 3 cut(s) | 197, 1782, 3813 |
| Eco24I | GRGCYC | 7 cut(s) | 1074, 1626, 2617, 3188, 3674, 5263, 5838 |
| Eco31I | GGTCTC | 1 cut(s) | 2629 |
| Eco32I | GATATC | 2 cut(s) | 339, 5294 |
| Eco47I | GGWCC | 5 cut(s) | 6, 177, 1838, 2813, 2878 |
| Eco53kI | GAGCTC | 3 cut(s) | 2615, 5261, 5836 |
| Eco72I | CACGTG | 1 cut(s) | 2237 |
| Eco88I | CYCGRG | 3 cut(s) | 1228, 2619, 3842 |
| EcoICRI | GAGCTC | 3 cut(s) | 2615, 5261, 5836 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| EcoO109I | RGGNCCY | 4 cut(s) | 858, 1070, 1071, 1838 |
| EcoRI | GAATTC | 1 cut(s) | 2101 |
| EcoRV | GATATC | 2 cut(s) | 339, 5294 |
| EcoT14I | CCWWGG | 3 cut(s) | 197, 1782, 3813 |
| EcoT22I | ATGCAT | 2 cut(s) | 3661, 4664 |
| EcoT38I | GRGCYC | 7 cut(s) | 1074, 1626, 2617, 3188, 3674, 5263, 5838 |
| ErhI | CCWWGG | 3 cut(s) | 197, 1782, 3813 |
| Esp3I | CGTCTC | 1 cut(s) | 778 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 813, 845, 1405, 1437, 2999, 3031 |
| FaqI | GGGAC | 7 cut(s) | 362, 995, 2332, 3725, 4758, 4847, 5118 |
| FauI | CCCGC | 1 cut(s) | 4457 |
| FauNDI | CATATG | 2 cut(s) | 665, 3424 |
| FbaI | TGATCA | 5 cut(s) | 78, 937, 1717, 1879, 5575 |
| FblI | GTMKAC | 4 cut(s) | 1300, 1686, 3779, 4729 |
| FriOI | GRGCYC | 7 cut(s) | 1074, 1626, 2617, 3188, 3674, 5263, 5838 |
| GlaI | GCGC | 4 cut(s) | 1364, 2586, 2603, 4649 |
| GsaI | CCCAGC | 2 cut(s) | 1701, 4377 |
| GsuI | CTGGAG | 6 cut(s) | 348, 1650, 4221, 4769, 5277, 5777 |
| HaeII | RGCGCY | 3 cut(s) | 1366, 2605, 4651 |
| HapII | CCGG | 4 cut(s) | 227, 1775, 4275, 5289 |
| HgaI | GACGC | 3 cut(s) | 1289, 2385, 4316 |
| HhaI | GCGC | 4 cut(s) | 1365, 2587, 2604, 4650 |
| Hin1I | GRCGYC | 1 cut(s) | 2396 |
| Hin6I | GCGC | 4 cut(s) | 1363, 2585, 2602, 4648 |
| HinP1I | GCGC | 4 cut(s) | 1363, 2585, 2602, 4648 |
| HincII | GTYRAC | 4 cut(s) | 1465, 1687, 2943, 3583 |
| HindII | GTYRAC | 4 cut(s) | 1465, 1687, 2943, 3583 |
| HindIII | AAGCTT | 1 cut(s) | 3753 |
| HpaI | GTTAAC | 1 cut(s) | 2943 |
| HpaII | CCGG | 4 cut(s) | 227, 1775, 4275, 5289 |
| Hpy99I | CGWCG | 1 cut(s) | 4146 |
| HpyCH4IV | ACGT | 6 cut(s) | 771, 1168, 1710, 2036, 2236, 3932 |
| HpySE526I | ACGT | 6 cut(s) | 771, 1168, 1710, 2036, 2236, 3932 |
| Hsp92I | GRCGYC | 1 cut(s) | 2396 |
| HspAI | GCGC | 4 cut(s) | 1363, 2585, 2602, 4648 |
| Kpn2I | TCCGGA | 1 cut(s) | 5288 |
| Ksp22I | TGATCA | 5 cut(s) | 78, 937, 1717, 1879, 5575 |
| KspAI | GTTAAC | 1 cut(s) | 2943 |
| LguI | GCTCTTC | 3 cut(s) | 468, 1858, 4810 |
| MaeII | ACGT | 6 cut(s) | 771, 1168, 1710, 2036, 2236, 3932 |
| MbiI | CCGCTC | 1 cut(s) | 1664 |
| MfeI | CAATTG | 4 cut(s) | 501, 4940, 5202, 5208 |
| MflI | RGATCY | 8 cut(s) | 202, 947, 1123, 1505, 3893, 3926, 4157, 4199 |
| MlsI | TGGCCA | 1 cut(s) | 4345 |
| MluNI | TGGCCA | 1 cut(s) | 4345 |
| MmeI | TCCRAC | 6 cut(s) | 51, 306, 1795, 3018, 3181, 5408 |
| Mox20I | TGGCCA | 1 cut(s) | 4345 |
| Mph1103I | ATGCAT | 2 cut(s) | 3661, 4664 |
| MroI | TCCGGA | 1 cut(s) | 5288 |
| MscI | TGGCCA | 1 cut(s) | 4345 |
| MslI | CAYNNNNRTG | 6 cut(s) | 122, 1326, 2168, 2580, 4220, 5312 |
| Msp20I | TGGCCA | 1 cut(s) | 4345 |
| MspA1I | CMGCKG | 5 cut(s) | 995, 1697, 3491, 4358, 5398 |
| MspCI | CTTAAG | 2 cut(s) | 3371, 4106 |
| MspI | CCGG | 4 cut(s) | 227, 1775, 4275, 5289 |
| MunI | CAATTG | 4 cut(s) | 501, 4940, 5202, 5208 |
| Mva1269I | GAATGC | 4 cut(s) | 610, 2384, 2681, 4279 |
| MvnI | CGCG | 2 cut(s) | 3304, 4464 |
| NciI | CCSGG | 2 cut(s) | 227, 4276 |
| NcoI | CCATGG | 2 cut(s) | 1782, 3813 |
| NdeI | CATATG | 2 cut(s) | 665, 3424 |
| NmeAIII | GCCGAG | 3 cut(s) | 1451, 1776, 1921 |
| NmuCI | GTSAC | 8 cut(s) | 851, 1535, 2912, 3055, 4048, 5040, 5197, 5358 |
| NsiI | ATGCAT | 2 cut(s) | 3661, 4664 |
| NspI | RCATGY | 9 cut(s) | 220, 548, 569, 1559, 2115, 3534, 4420, 5371, 5409 |
| NspV | TTCGAA | 1 cut(s) | 1752 |
| OliI | CACNNNNGTG | 2 cut(s) | 1326, 5312 |
| PaeI | GCATGC | 3 cut(s) | 220, 548, 5371 |
| PaeR7I | CTCGAG | 1 cut(s) | 1228 |
| PagI | TCATGA | 1 cut(s) | 5613 |
| PasI | CCCWGGG | 1 cut(s) | 4830 |
| PciI | ACATGT | 2 cut(s) | 4416, 5405 |
| PciSI | GCTCTTC | 3 cut(s) | 468, 1858, 4810 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1165, 4150 |
| PctI | GAATGC | 4 cut(s) | 610, 2384, 2681, 4279 |
| PflFI | GACNNNGTC | 1 cut(s) | 1384 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1673 |
| PfoI | TCCNGGA | 2 cut(s) | 2116, 5254 |
| Ple19I | CGATCG | 1 cut(s) | 1327 |
| PmaCI | CACGTG | 1 cut(s) | 2237 |
| PmlI | CACGTG | 1 cut(s) | 2237 |
| Ppu21I | YACGTR | 2 cut(s) | 2037, 2237 |
| PpuMI | RGGWCCY | 1 cut(s) | 1838 |
| PscI | ACATGT | 2 cut(s) | 4416, 5405 |
| PshBI | ATTAAT | 2 cut(s) | 2753, 4899 |
| Psp124BI | GAGCTC | 3 cut(s) | 2617, 5263, 5838 |
| Psp5II | RGGWCCY | 1 cut(s) | 1838 |
| PspCI | CACGTG | 1 cut(s) | 2237 |
| PspFI | CCCAGC | 2 cut(s) | 1697, 4373 |
| PspOMI | GGGCCC | 1 cut(s) | 1070 |
| PspPPI | RGGWCCY | 1 cut(s) | 1838 |
| PspXI | VCTCGAGB | 1 cut(s) | 1228 |
| PstI | CTGCAG | 8 cut(s) | 1059, 1639, 2997, 3294, 4044, 4357, 4776, 4797 |
| PstNI | CAGNNNCTG | 5 cut(s) | 385, 1634, 1673, 1841, 4792 |
| PsuI | RGATCY | 8 cut(s) | 202, 947, 1123, 1505, 3893, 3926, 4157, 4199 |
| PsyI | GACNNNGTC | 1 cut(s) | 1384 |
| PvuI | CGATCG | 1 cut(s) | 1327 |
| PvuII | CAGCTG | 4 cut(s) | 995, 1697, 4358, 5398 |
| RseI | CAYNNNNRTG | 6 cut(s) | 122, 1326, 2168, 2580, 4220, 5312 |
| SacI | GAGCTC | 3 cut(s) | 2617, 5263, 5838 |
| SalI | GTCGAC | 1 cut(s) | 1685 |
| SapI | GCTCTTC | 3 cut(s) | 468, 1858, 4810 |
| ScaI | AGTACT | 3 cut(s) | 603, 3154, 4008 |
| Sfr274I | CTCGAG | 1 cut(s) | 1228 |
| SfuI | TTCGAA | 1 cut(s) | 1752 |
| SinI | GGWCC | 5 cut(s) | 6, 177, 1838, 2813, 2878 |
| SlaI | CTCGAG | 1 cut(s) | 1228 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 122, 1326, 2168, 2580, 4220, 5312 |
| SpeI | ACTAGT | 1 cut(s) | 4076 |
| SphI | GCATGC | 3 cut(s) | 220, 548, 5371 |
| SsiI | CCGC | 9 cut(s) | 1662, 2679, 3076, 3304, 3489, 4442, 4464, 4945, 5764 |
| SspI | AATATT | 1 cut(s) | 537 |
| SstI | GAGCTC | 3 cut(s) | 2617, 5263, 5838 |
| StyI | CCWWGG | 3 cut(s) | 197, 1782, 3813 |
| TaiI | ACGT | 6 cut(s) | 774, 1171, 1713, 2039, 2239, 3935 |
| TaqI | TCGA | 9 cut(s) | 1229, 1369, 1686, 1752, 2627, 3139, 3234, 3471, 4144 |
| TaqII | GACCGA | 1 cut(s) | 3328 |
| TatI | WGTACW | 5 cut(s) | 601, 2227, 2758, 3152, 4006 |
| TauI | GCSGC | 4 cut(s) | 3078, 3307, 3491, 4948 |
| TseFI | GTSAC | 8 cut(s) | 851, 1535, 2912, 3055, 4048, 5040, 5197, 5358 |
| Tsp45I | GTSAC | 8 cut(s) | 851, 1535, 2912, 3055, 4048, 5040, 5197, 5358 |
| TspGWI | ACGGA | 2 cut(s) | 2211, 3886 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1384 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1673 |
| Vha464I | CTTAAG | 2 cut(s) | 3371, 4106 |
| VneI | GTGCAC | 1 cut(s) | 5136 |
| VpaK11BI | GGWCC | 5 cut(s) | 6, 177, 1838, 2813, 2878 |
| VspI | ATTAAT | 2 cut(s) | 2753, 4899 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| XapI | RAATTY | 6 cut(s) | 506, 622, 1874, 2101, 2335, 5669 |
| XbaI | TCTAGA | 1 cut(s) | 1994 |
| XceI | RCATGY | 9 cut(s) | 220, 548, 569, 1559, 2115, 3534, 4420, 5371, 5409 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 589, 2174, 2888 |
| XhoI | CTCGAG | 1 cut(s) | 1228 |
| XmiI | GTMKAC | 4 cut(s) | 1300, 1686, 3779, 4729 |
| ZrmI | AGTACT | 3 cut(s) | 603, 3154, 4008 |
| Zsp2I | ATGCAT | 2 cut(s) | 3661, 4664 |