AT1G61320

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016662
Physical Location & Seq
Forward (+)
22617370 .. 22619853
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61320.1

Sequence Viewer

Length: 1380 bp
ATGGCTCCTCCTACCAAGAGAACAAGAGTAGAAATGGCAGAATCATCAAATAAGAGGATGAAACCTAGTGAAACGGTTCCTGAAGATGTGTTGGAACTCATGATGTCTACGTATCTTCCCGTCCAATCTCTGTTAACAACTCGTGTGTTGTCCAAAAGATTTCGCGAGACCGAGGTACGTTCTCTTGACCTCGATTTCAGTGGAATATATTCTAGAAGACGTCGTAAAGTAGAAGTTGTTGGCATCATCGAAAAAGTGTTCAATCAACATAAAGGATCAGAGATTCACCGATTCGTTCTACTTCTCAATCATATTGGGGTAGAAGATAAAATCATCTCATGGACCAATACATGTTTTGACAAAAACATCAAGGAGCTTGTCTTAGATTTTTCAAAATCTAGAAAAGTTATGGCCATCCCCATTGATTTCTCGGCTGTGGAGAGTCTACAAGTTTTGAAGCTGCGATGGTGTAAATTTGAAATACCTGATAGTTCCCCAAAAGGTCTAAAGCTCTTGAAGACACTCTCGCTTATGCGTACCCAAGTGATGGTGAAGACTATCGATGCAATCTTCAACAACTGCATCCACCTAGAGTCGCTTGAACTAATCGAATGCCGTATGGATGGGATATTAAGCATACGTGCTCAGAATCATAAGAAGTTCAAGTCGCTTGTTGTGTCTTTTATGCCGGATCTCCGACACATCCGGTTGGACGCACCCACTTTAGAAAACTACAAGTATGATGGATATGTCATTTGTGTTAACATTCTGATAACAAATGCACTTAAAGAGGCAAATCTCTACTACACTCGGATTCGGCGTTTATACCACCAAAAATCTGATTTGGTGGATACACTGCGATTCTATACAAGACTTACTGTCCTCGCAACGACAACCATCTTTCTTGAGGCTCTTACGAAGAGATACGTCGGAGAAGGAAGACTAGAAAATCCGCCTTTCAAGTTTGAGAACTTAACAGAATTCAAGATTTCCTTTATAACTCCAACATTTTGCACTCTATTTGACATCGCTGAATTCCTCAAAGAGTGCCCTAAACTCAAACAGGTTGTGATCGATATTCAGAATTTCACATTTGAACCTCAAATGTATTTTTGGGAGATACATCATAAGGCGCAGATTCAGAATACTTCGAATAACAACTACCTTTTGAAGTGTCTCACGGACGTGAAGATCATTGGCTATAAAGGCCACTGGCATGAGCTTGATATAGTAGAGTTCTTTGTCAAGAACGCACCATCTCTAAAGAGGTTGGAGTTACAGATGCCTAAGAACGCGAAGAACGATGCTCACACCCCAGATGTTGCAAGGATAAAGCTCATAAAAACCATCTTTTCAGGGGTTAAAGTTACCGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

459

Amino Acids

53.54

Weight (kDa)

9.33

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 87 - 433 3.1e-23 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 113 - 245 2.9e-13 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61320 AT1G61330
malus_domestica MD10G1303300.v1.1
prunus_persica Prupe.4G036700_v2.0.a1
pyrus_communis pycom10g25580
rosa_laevigata RLG00000019732
rosa_roxburghii Rroxscaffold_1G00069650 Rroxscaffold_1G00069660
rosa_rugosa Rorug04G0421300
rosa_samantha Rh2BG409000 Rh5BG048300 Rh5BG427600 Rh5DG047400 Rh5DG047500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
AatII GACGTC 1 cut(s) 223
AccB7I CCANNNNNTGG 1 cut(s) 547
AccI GTMKAC 2 cut(s) 107, 445
AccII CGCG 2 cut(s) 165, 1295
AciI CCGC 1 cut(s) 953
AclWI GGATC 2 cut(s) 283, 699
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 4 cut(s) 473, 980, 1034, 1084
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 220
AfaI GTAC 2 cut(s) 177, 538
AfiI CCNNNNNNNGG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 350
AhdI GACNNNNNGTC 1 cut(s) 878
AjiI CACGTC 1 cut(s) 1186
AleI CACNNNNGTG 1 cut(s) 1184
AluBI AGCT 5 cut(s) 376, 460, 511, 1222, 1336
AluI AGCT 5 cut(s) 376, 460, 511, 1222, 1336
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 2 cut(s) 161, 1181
AlwI GGATC 2 cut(s) 283, 699
AoxI GGCC 2 cut(s) 411, 1207
ApeKI GCWGC 1 cut(s) 460
ApoI RAATTY 4 cut(s) 473, 980, 1034, 1084
Asp700I GAANNNNTTC 2 cut(s) 75, 208
AspLEI GCGC 1 cut(s) 1135
AspS9I GGNCC 1 cut(s) 342
AsuHPI GGTGA 2 cut(s) 278, 562
AsuII TTCGAA 1 cut(s) 1151
AvaII GGWCC 1 cut(s) 342
BaeGI GKGCMC 1 cut(s) 1052
BalI TGGCCA 1 cut(s) 413
BauI CACGAG 1 cut(s) 141
BbsI GAAGAC 4 cut(s) 223, 524, 560, 946
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 1 cut(s) 447
BccI CCATC 8 cut(s) 422, 459, 541, 617, 737, 905, 1264, 1355
BceAI ACGGC 1 cut(s) 600
BciVI GTATCC 1 cut(s) 844
BcoDI GTCTC 2 cut(s) 161, 1181
BfaI CTAG 5 cut(s) 66, 213, 399, 590, 944
BfuI GTATCC 1 cut(s) 844
BisI GCNGC 1 cut(s) 461
BlsI GCNGC 1 cut(s) 462
Bme18I GGWCC 1 cut(s) 342
BmeRI GACNNNNNGTC 1 cut(s) 878
BmgBI CACGTC 1 cut(s) 1186
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 2 cut(s) 6, 78
BmsI GCATC 5 cut(s) 252, 553, 591, 1272, 1294
BpiI GAAGAC 4 cut(s) 223, 524, 560, 946
Bpu14I TTCGAA 1 cut(s) 1151
BpuEI CTTGAG 1 cut(s) 926
Bsa29I ATCGAT 2 cut(s) 561, 1074
BsaAI YACGTR 2 cut(s) 111, 641
BsaHI GRCGYC 1 cut(s) 220
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 705
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 1217
BseCI ATCGAT 2 cut(s) 561, 1074
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 5 cut(s) 63, 414, 582, 628, 702
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 659
BseNI ACTGG 1 cut(s) 1217
BseSI GKGCMC 1 cut(s) 1052
BseXI GCAGC 1 cut(s) 447
Bsh1236I CGCG 2 cut(s) 165, 1295
BshFI GGCC 2 cut(s) 413, 1209
BshVI ATCGAT 2 cut(s) 561, 1074
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 2 cut(s) 689, 706
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 2 cut(s) 161, 1181
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 2 cut(s) 413, 1209
Bso31I GGTCTC 1 cut(s) 161
Bsp119I TTCGAA 1 cut(s) 1151
Bsp1286I GDGCHC 2 cut(s) 646, 1052
Bsp143I GATC 4 cut(s) 275, 691, 1071, 1191
Bsp68I TCGCGA 1 cut(s) 165
BspACI CCGC 1 cut(s) 953
BspANI GGCC 2 cut(s) 413, 1209
BspCNI CTCAG 1 cut(s) 658
BspDI ATCGAT 2 cut(s) 561, 1074
BspFNI CGCG 2 cut(s) 165, 1295
BspHI TCATGA 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 6, 78
BspPI GGATC 2 cut(s) 283, 699
BspT104I TTCGAA 1 cut(s) 1151
BspTNI GGTCTC 1 cut(s) 161
BsrI ACTGG 1 cut(s) 1217
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 4 cut(s) 275, 691, 1071, 1191
BssNI GRCGYC 1 cut(s) 220
BssSI CACGAG 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 76, 880
Bst6I CTCTTC 1 cut(s) 914
BstACI GRCGYC 1 cut(s) 220
BstBAI YACGTR 2 cut(s) 111, 641
BstBI TTCGAA 1 cut(s) 1151
BstDEI CTNAG 3 cut(s) 382, 645, 1287
BstF5I GGATG 5 cut(s) 63, 414, 582, 628, 702
BstFNI CGCG 2 cut(s) 165, 1295
BstHHI GCGC 1 cut(s) 1135
BstKTI GATC 4 cut(s) 278, 694, 1074, 1194
BstMAI GTCTC 2 cut(s) 161, 1181
BstMBI GATC 4 cut(s) 275, 691, 1071, 1191
BstMWI GCNNNNNNNGC 1 cut(s) 1206
BstNSI RCATGY 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 1052
BstSNI TACGTA 1 cut(s) 111
BstUI CGCG 2 cut(s) 165, 1295
BstV1I GCAGC 1 cut(s) 447
BstV2I GAAGAC 4 cut(s) 223, 524, 560, 946
BstX2I RGATCY 1 cut(s) 691
BstYI RGATCY 1 cut(s) 691
Bsu15I ATCGAT 2 cut(s) 561, 1074
BsuI GTATCC 1 cut(s) 844
BsuRI GGCC 2 cut(s) 413, 1209
BsuTUI ATCGAT 2 cut(s) 561, 1074
BtgZI GCGATG 2 cut(s) 478, 1012
BtrI CACGTC 1 cut(s) 1186
BtsCI GGATG 5 cut(s) 63, 414, 582, 628, 702
BtsI GCAGTG 1 cut(s) 854
BtsIMutI CAGTG 3 cut(s) 205, 854, 1210
BtuMI TCGCGA 1 cut(s) 165
CciI TCATGA 1 cut(s) 99
CfoI GCGC 1 cut(s) 1135
Cfr13I GGNCC 1 cut(s) 342
ClaI ATCGAT 2 cut(s) 561, 1074
CseI GACGC 1 cut(s) 722
Csp6I GTAC 2 cut(s) 176, 537
CviAII CATG 4 cut(s) 100, 339, 351, 1217
CviQI GTAC 2 cut(s) 176, 537
DdeI CTNAG 3 cut(s) 382, 645, 1287
DpnI GATC 4 cut(s) 277, 693, 1073, 1193
DpnII GATC 4 cut(s) 275, 691, 1071, 1191
DriI GACNNNNNGTC 1 cut(s) 878
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 1 cut(s) 914
Eam1105I GACNNNNNGTC 1 cut(s) 878
EarI CTCTTC 1 cut(s) 914
EciI GGCGGA 1 cut(s) 942
Eco105I TACGTA 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 342
Eco57I CTGAAG 1 cut(s) 102
EcoRI GAATTC 2 cut(s) 980, 1034
FaeI CATG 4 cut(s) 103, 342, 354, 1220
FalI AAGNNNNNCTT 2 cut(s) 977, 1009
FatI CATG 4 cut(s) 99, 338, 350, 1216
FblI GTMKAC 2 cut(s) 107, 445
Fnu4HI GCNGC 1 cut(s) 461
FokI GGATG 5 cut(s) 70, 401, 569, 635, 689
Fsp4HI GCNGC 1 cut(s) 461
FspBI CTAG 5 cut(s) 66, 213, 399, 590, 944
GlaI GCGC 1 cut(s) 1134
GluI GCNGC 1 cut(s) 461
HaeIII GGCC 2 cut(s) 413, 1209
HapII CCGG 2 cut(s) 689, 706
HgaI GACGC 1 cut(s) 722
HhaI GCGC 1 cut(s) 1135
Hin1I GRCGYC 1 cut(s) 220
Hin1II CATG 4 cut(s) 103, 342, 354, 1220
Hin6I GCGC 1 cut(s) 1133
HinP1I GCGC 1 cut(s) 1133
HincII GTYRAC 2 cut(s) 135, 763
HindII GTYRAC 2 cut(s) 135, 763
HinfI GANTC 9 cut(s) 41, 283, 291, 442, 593, 649, 814, 861, 1138
HpaI GTTAAC 2 cut(s) 135, 763
HpaII CCGG 2 cut(s) 689, 706
HphI GGTGA 2 cut(s) 278, 562
Hpy166II GTNNAC 4 cut(s) 108, 135, 446, 763
Hpy188I TCNGA 9 cut(s) 280, 648, 698, 771, 813, 841, 932, 1083, 1143
Hpy8I GTNNAC 4 cut(s) 108, 135, 446, 763
Hpy99I CGWCG 2 cut(s) 225, 932
HpyAV CCTTC 1 cut(s) 929
HpyCH4III ACNGT 2 cut(s) 76, 880
HpyCH4IV ACGT 6 cut(s) 110, 178, 220, 640, 927, 1185
HpyCH4V TGCA 5 cut(s) 566, 582, 782, 1014, 1325
HpyF10VI GCNNNNNNNGC 1 cut(s) 1206
HpyF3I CTNAG 3 cut(s) 382, 645, 1287
HpySE526I ACGT 6 cut(s) 110, 178, 220, 640, 927, 1185
Hsp92I GRCGYC 1 cut(s) 220
Hsp92II CATG 4 cut(s) 103, 342, 354, 1220
HspAI GCGC 1 cut(s) 1133
KspAI GTTAAC 2 cut(s) 135, 763
Kzo9I GATC 4 cut(s) 275, 691, 1071, 1191
LmnI GCTCC 2 cut(s) 10, 373
LpnPI CCDG 8 cut(s) 93, 498, 702, 719, 1049, 1198, 1329, 1341
Lsp1109I GCAGC 1 cut(s) 447
LweI GCATC 5 cut(s) 252, 553, 591, 1272, 1294
MaeI CTAG 5 cut(s) 66, 213, 399, 590, 944
MaeII ACGT 6 cut(s) 110, 178, 220, 640, 927, 1185
MaeIII GTNAC 2 cut(s) 1275, 1366
MalI GATC 4 cut(s) 277, 693, 1073, 1193
MboI GATC 4 cut(s) 275, 691, 1071, 1191
MflI RGATCY 1 cut(s) 691
MhlI GDGCHC 2 cut(s) 646, 1052
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 4 cut(s) 473, 980, 1034, 1084
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 2 cut(s) 451, 602
MmeI TCCRAC 6 cut(s) 72, 690, 721, 910, 1028, 1251
Mox20I TGGCCA 1 cut(s) 413
MroXI GAANNNNTTC 2 cut(s) 75, 208
MscI TGGCCA 1 cut(s) 413
MseI TTAA 7 cut(s) 134, 632, 762, 786, 974, 1362, 1378
MslI CAYNNNNRTG 2 cut(s) 1184, 1215
Msp20I TGGCCA 1 cut(s) 413
MspI CCGG 2 cut(s) 689, 706
Mva1269I GAATGC 1 cut(s) 617
MvnI CGCG 2 cut(s) 165, 1295
MwoI GCNNNNNNNGC 1 cut(s) 1206
NdeII GATC 4 cut(s) 275, 691, 1071, 1191
NlaIII CATG 4 cut(s) 103, 342, 354, 1220
NlaIV GGNNCC 2 cut(s) 6, 78
NmeAIII GCCGAG 1 cut(s) 410
NruI TCGCGA 1 cut(s) 165
NspI RCATGY 1 cut(s) 354
NspV TTCGAA 1 cut(s) 1151
OliI CACNNNNGTG 1 cut(s) 1184
PagI TCATGA 1 cut(s) 99
PciI ACATGT 1 cut(s) 350
PcsI WCGNNNNNNNCGW 2 cut(s) 817, 1299
PctI GAATGC 1 cut(s) 617
PdmI GAANNNNTTC 2 cut(s) 75, 208
PfeI GAWTC 7 cut(s) 41, 283, 291, 649, 814, 861, 1138
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 1 cut(s) 462
PleI GAGTC 2 cut(s) 450, 601
PpsI GAGTC 2 cut(s) 450, 601
Ppu21I YACGTR 2 cut(s) 111, 641
PscI ACATGT 1 cut(s) 350
PsiI TTATAA 1 cut(s) 998
PspN4I GGNNCC 2 cut(s) 6, 78
PspPI GGNCC 1 cut(s) 342
PsuI RGATCY 1 cut(s) 691
RruI TCGCGA 1 cut(s) 165
RsaI GTAC 2 cut(s) 177, 538
RsaNI GTAC 2 cut(s) 176, 537
RseI CAYNNNNRTG 2 cut(s) 1184, 1215
SaqAI TTAA 7 cut(s) 134, 632, 762, 786, 974, 1362, 1378
SatI GCNGC 1 cut(s) 461
Sau3AI GATC 4 cut(s) 275, 691, 1071, 1191
Sau96I GGNCC 1 cut(s) 342
SchI GAGTC 2 cut(s) 451, 602
SduI GDGCHC 2 cut(s) 646, 1052
SfaNI GCATC 5 cut(s) 252, 553, 591, 1272, 1294
SfuI TTCGAA 1 cut(s) 1151
SinI GGWCC 1 cut(s) 342
SmiMI CAYNNNNRTG 2 cut(s) 1184, 1215
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
SnaBI TACGTA 1 cut(s) 111
Sse9I AATT 4 cut(s) 473, 980, 1034, 1084
SsiI CCGC 1 cut(s) 953
SspMI CTAG 5 cut(s) 66, 213, 399, 590, 944
TaaI ACNGT 2 cut(s) 76, 880
TaiI ACGT 6 cut(s) 113, 181, 223, 643, 930, 1188
TaqI TCGA 6 cut(s) 192, 249, 561, 609, 1074, 1151
TaqII GACCGA 1 cut(s) 185
TasI AATT 4 cut(s) 473, 980, 1034, 1084
TfiI GAWTC 7 cut(s) 41, 283, 291, 649, 814, 861, 1138
Tru1I TTAA 7 cut(s) 134, 632, 762, 786, 974, 1362, 1378
Tru9I TTAA 7 cut(s) 134, 632, 762, 786, 974, 1362, 1378
TscAI CASTG 3 cut(s) 205, 861, 1217
TseI GCWGC 1 cut(s) 460
TspDTI ATGAA 1 cut(s) 74
TspGWI ACGGA 1 cut(s) 1196
TspRI CASTG 3 cut(s) 205, 861, 1217
Van91I CCANNNNNTGG 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 4 cut(s) 473, 980, 1034, 1084
XbaI TCTAGA 2 cut(s) 212, 398
XceI RCATGY 1 cut(s) 354
XmiI GTMKAC 2 cut(s) 107, 445
XmnI GAANNNNTTC 2 cut(s) 75, 208
XspI CTAG 5 cut(s) 66, 213, 399, 590, 944
ZraI GACGTC 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.