Rh2BG409000

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016662
Physical Location & Seq
Forward (+)
57957714 .. 57986917
29204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG409000.1

Sequence Viewer

Length: 615 bp
ATGAGAAGGAGGCGGAGGGGTCACGATCCACGTGGAGGAGGCGGGGGCCCTAAACTCGCCTCCACCGTCACCTCCGCCGCGTGCATCGGTGACCCCTCCGCCGCCTCCACGTGGACCAGGGAGTGCCGCTGCAGATCCGTGCGTGCAGATAGGGATGGTGGCCAAAGAGTTTGGGGAACATGGCAGAAATGCCGAATTCTCGACTTCGGGAGAGAATTCGCGGCCACCCAACCCCACCGGTTGGCTTACAGCGCCGCCGTGGATCATGCCATTGAATCCTACCACAAAGAGGGAAAGATCAAAACCCTCCAGTTATGTTTCGATCCGTCCAATGTTGAGTTTCTTCTTGAGAAGTGGGTTCGGAAATCGGTCAAGAAAGGAGTCGAGGAGCTCGATCTCGACATATTTCAGGCGACGGTGCCGTTTTTTGTCCCTACTAATGCTGCAGAGGCAAAGCTAAAAGAGCTTAAGCTCTATTATTCGGTGATCCATATGCTATTTAATTTGAAGGCTCTGGTATCTCTGACCACCTTGGTTTTGAGGCAGATTGATCTGACGGACCTGATCATTGAGGTACGTTGTGTTCAAGAACTGTGTCCATCTCGAGACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

204

Amino Acids

23.03

Weight (kDa)

9.24

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 95 - 187 6.4e-07 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61320 AT1G61330
malus_domestica MD10G1303300.v1.1
prunus_persica Prupe.4G036700_v2.0.a1
pyrus_communis pycom10g25580
rosa_laevigata RLG00000019732
rosa_roxburghii Rroxscaffold_1G00069650 Rroxscaffold_1G00069660
rosa_rugosa Rorug04G0421300
rosa_samantha Rh2BG409000 Rh5BG048300 Rh5BG427600 Rh5DG047400 Rh5DG047500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 241
AccII CGCG 2 cut(s) 80, 221
AciI CCGC 9 cut(s) 13, 42, 75, 78, 99, 102, 127, 221, 255
AclWI GGATC 5 cut(s) 20, 129, 270, 317, 481
AcoI YGGCCR 2 cut(s) 160, 222
AcsI RAATTY 2 cut(s) 195, 215
AcvI CACGTG 2 cut(s) 32, 111
AfaI GTAC 1 cut(s) 576
AfiI CCNNNNNNNGG 4 cut(s) 35, 111, 241, 289
AflII CTTAAG 1 cut(s) 467
AgeI ACCGGT 1 cut(s) 237
AgsI TTSAA 3 cut(s) 275, 508, 587
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 4 cut(s) 391, 457, 466, 472
AluI AGCT 4 cut(s) 391, 457, 466, 472
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 5 cut(s) 20, 129, 270, 317, 481
Ama87I CYCGRG 1 cut(s) 603
AoxI GGCC 3 cut(s) 46, 160, 222
ApaI GGGCCC 1 cut(s) 50
ApeKI GCWGC 2 cut(s) 129, 443
ApoI RAATTY 2 cut(s) 195, 215
AsiGI ACCGGT 1 cut(s) 237
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 4 cut(s) 46, 47, 114, 559
AsuHPI GGTGA 3 cut(s) 61, 101, 496
AvaI CYCGRG 1 cut(s) 603
AvaII GGWCC 2 cut(s) 114, 559
BaeGI GKGCMC 1 cut(s) 50
BalI TGGCCA 1 cut(s) 162
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 2 cut(s) 50, 393
BbrPI CACGTG 2 cut(s) 32, 111
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 116, 430
BccI CCATC 2 cut(s) 149, 607
BceAI ACGGC 2 cut(s) 242, 406
BciT130I CCWGG 1 cut(s) 118
BclI TGATCA 1 cut(s) 564
BcoDI GTCTC 1 cut(s) 600
BfmI CTRYAG 2 cut(s) 130, 444
BfoI RGCGCY 1 cut(s) 255
BfrI CTTAAG 1 cut(s) 467
BisI GCNGC 7 cut(s) 78, 102, 127, 130, 222, 255, 444
BlsI GCNGC 7 cut(s) 79, 103, 128, 131, 223, 256, 445
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 2 cut(s) 114, 559
BmeT110I CYCGRG 1 cut(s) 603
BmgT120I GGNCC 4 cut(s) 46, 47, 114, 559
BmiI GGNNCC 3 cut(s) 47, 48, 420
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 368
BsaAI YACGTR 2 cut(s) 32, 111
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 3 cut(s) 117, 258, 531
BsaWI WCCGGW 1 cut(s) 237
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 111, 241, 289
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 117, 258, 531
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 4 cut(s) 35, 111, 241, 289
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 2 cut(s) 51, 401
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 2 cut(s) 116, 430
BsgI GTGCAG 1 cut(s) 165
Bsh1236I CGCG 2 cut(s) 80, 221
BshFI GGCC 3 cut(s) 48, 162, 224
BshNI GGYRCC 1 cut(s) 418
BshTI ACCGGT 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 603
BsiSI CCGG 1 cut(s) 238
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 35, 111, 241, 289
BsmAI GTCTC 1 cut(s) 600
BsmFI GGGAC 1 cut(s) 416
BsnI GGCC 3 cut(s) 48, 162, 224
Bso31I GGTCTC 1 cut(s) 600
BsoBI CYCGRG 1 cut(s) 603
Bsp120I GGGCCC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 50, 393
Bsp143I GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
BspACI CCGC 9 cut(s) 13, 42, 75, 78, 99, 102, 127, 221, 255
BspANI GGCC 3 cut(s) 48, 162, 224
BspFNI CGCG 2 cut(s) 80, 221
BspLI GGNNCC 3 cut(s) 47, 48, 420
BspMAI CTGCAG 2 cut(s) 134, 448
BspPI GGATC 5 cut(s) 20, 129, 270, 317, 481
BspT107I GGYRCC 1 cut(s) 418
BspTI CTTAAG 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 3 cut(s) 117, 258, 531
BssMI GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 3 cut(s) 67, 418, 594
BstAFI CTTAAG 1 cut(s) 467
BstBAI YACGTR 2 cut(s) 32, 111
BstC8I GCNNGC 2 cut(s) 82, 144
BstDSI CCRYGG 1 cut(s) 258
BstEII GGTNACC 1 cut(s) 89
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 2 cut(s) 80, 221
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 9 cut(s) 28, 137, 265, 300, 325, 397, 489, 553, 567
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
BstMWI GCNNNNNNNGC 3 cut(s) 251, 449, 463
BstNI CCWGG 1 cut(s) 118
BstPI GGTNACC 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 2 cut(s) 130, 444
BstSLI GKGCMC 1 cut(s) 50
BstUI CGCG 2 cut(s) 80, 221
BstV1I GCAGC 2 cut(s) 116, 430
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BsuRI GGCC 3 cut(s) 48, 162, 224
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 160
Cac8I GCNNGC 2 cut(s) 82, 144
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 4 cut(s) 46, 47, 114, 559
Csp6I GTAC 1 cut(s) 575
CspAI ACCGGT 1 cut(s) 237
CviAII CATG 2 cut(s) 180, 266
CviJI RGCY 9 cut(s) 48, 162, 224, 245, 391, 457, 466, 472, 512
CviKI_1 RGCY 9 cut(s) 48, 162, 224, 245, 391, 457, 466, 472, 512
CviQI GTAC 1 cut(s) 575
DpnI GATC 9 cut(s) 27, 136, 264, 299, 324, 396, 488, 552, 566
DpnII GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
EaeI YGGCCR 2 cut(s) 160, 222
EciI GGCGGA 3 cut(s) 28, 64, 88
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 2 cut(s) 50, 393
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 114, 559
Eco53kI GAGCTC 1 cut(s) 391
Eco72I CACGTG 2 cut(s) 32, 111
Eco88I CYCGRG 1 cut(s) 603
Eco91I GGTNACC 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 391
EcoO109I RGGNCCY 2 cut(s) 46, 47
EcoO65I GGTNACC 1 cut(s) 89
EcoRI GAATTC 2 cut(s) 195, 215
EcoRII CCWGG 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 2 cut(s) 50, 393
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 2 cut(s) 183, 269
FaiI YATR 6 cut(s) 181, 267, 316, 404, 492, 494
FaqI GGGAC 1 cut(s) 416
FatI CATG 2 cut(s) 179, 265
FauI CCCGC 1 cut(s) 35
FauNDI CATATG 1 cut(s) 492
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 7 cut(s) 78, 102, 127, 130, 222, 255, 444
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 2 cut(s) 50, 393
Fsp4HI GCNGC 7 cut(s) 78, 102, 127, 130, 222, 255, 444
GlaI GCGC 1 cut(s) 253
GluI GCNGC 7 cut(s) 78, 102, 127, 130, 222, 255, 444
GsuI CTGGAG 1 cut(s) 293
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 3 cut(s) 48, 162, 224
HapII CCGG 1 cut(s) 238
HhaI GCGC 1 cut(s) 254
Hin1II CATG 2 cut(s) 183, 269
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 2 cut(s) 275, 381
HpaII CCGG 1 cut(s) 238
HphI GGTGA 3 cut(s) 61, 101, 496
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 3 cut(s) 363, 525, 555
Hpy188III TCNNGA 9 cut(s) 23, 200, 208, 347, 373, 398, 587, 603, 605
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 1 cut(s) 418
HpyAV CCTTC 1 cut(s) 502
HpyCH4III ACNGT 3 cut(s) 67, 418, 594
HpyCH4IV ACGT 3 cut(s) 31, 110, 577
HpyCH4V TGCA 4 cut(s) 84, 132, 146, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 449, 463
HpySE526I ACGT 3 cut(s) 31, 110, 577
Hsp92II CATG 2 cut(s) 183, 269
HspAI GCGC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
LmnI GCTCC 1 cut(s) 388
LpnPI CCDG 7 cut(s) 103, 130, 251, 323, 395, 500, 575
Lsp1109I GCAGC 2 cut(s) 116, 430
LweI GCATC 1 cut(s) 93
MaeII ACGT 3 cut(s) 31, 110, 577
MaeIII GTNAC 3 cut(s) 20, 67, 89
MalI GATC 9 cut(s) 27, 136, 264, 299, 324, 396, 488, 552, 566
MboI GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
MboII GAAGA 1 cut(s) 335
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 2 cut(s) 50, 393
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 3 cut(s) 195, 215, 502
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 1 cut(s) 390
Mox20I TGGCCA 1 cut(s) 162
MscI TGGCCA 1 cut(s) 162
MseI TTAA 2 cut(s) 468, 501
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 129
MspCI CTTAAG 1 cut(s) 467
MspI CCGG 1 cut(s) 238
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 2 cut(s) 80, 221
MwoI GCNNNNNNNGC 3 cut(s) 251, 449, 463
NdeI CATATG 1 cut(s) 492
NdeII GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
NlaIII CATG 2 cut(s) 183, 269
NlaIV GGNNCC 3 cut(s) 47, 48, 420
NmuCI GTSAC 3 cut(s) 20, 67, 89
PaeR7I CTCGAG 1 cut(s) 603
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 390
PfeI GAWTC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 241
PinAI ACCGGT 1 cut(s) 237
PkrI GCNGC 7 cut(s) 79, 103, 128, 131, 223, 256, 445
PleI GAGTC 1 cut(s) 389
PmaCI CACGTG 2 cut(s) 32, 111
PmlI CACGTG 2 cut(s) 32, 111
PpsI GAGTC 1 cut(s) 389
Ppu21I YACGTR 2 cut(s) 32, 111
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 116
PspCI CACGTG 2 cut(s) 32, 111
PspEI GGTNACC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 47, 48, 420
PspOMI GGGCCC 1 cut(s) 46
PspPI GGNCC 4 cut(s) 46, 47, 114, 559
PsrI GAACNNNNNNTAC 2 cut(s) 567, 599
PstI CTGCAG 2 cut(s) 134, 448
PsuI RGATCY 1 cut(s) 134
RsaI GTAC 1 cut(s) 576
RsaNI GTAC 1 cut(s) 575
SacI GAGCTC 1 cut(s) 393
SaqAI TTAA 2 cut(s) 468, 501
SatI GCNGC 7 cut(s) 78, 102, 127, 130, 222, 255, 444
Sau3AI GATC 9 cut(s) 25, 134, 262, 297, 322, 394, 486, 550, 564
Sau96I GGNCC 4 cut(s) 46, 47, 114, 559
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 2 cut(s) 50, 393
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 2 cut(s) 130, 444
Sfr274I CTCGAG 1 cut(s) 603
SinI GGWCC 2 cut(s) 114, 559
SlaI CTCGAG 1 cut(s) 603
SmlI CTYRAG 3 cut(s) 347, 467, 603
SmoI CTYRAG 3 cut(s) 347, 467, 603
Sse9I AATT 3 cut(s) 195, 215, 502
SsiI CCGC 9 cut(s) 13, 42, 75, 78, 99, 102, 127, 221, 255
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 3 cut(s) 67, 418, 594
TaiI ACGT 3 cut(s) 34, 113, 580
TaqI TCGA 6 cut(s) 201, 321, 384, 393, 399, 604
TaqII GACCGA 1 cut(s) 358
TasI AATT 3 cut(s) 195, 215, 502
TauI GCSGC 5 cut(s) 80, 104, 129, 224, 257
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 468, 501
Tru9I TTAA 2 cut(s) 468, 501
TseFI GTSAC 3 cut(s) 20, 67, 89
TseI GCWGC 2 cut(s) 129, 443
Tsp45I GTSAC 3 cut(s) 20, 67, 89
TspGWI ACGGA 3 cut(s) 127, 315, 572
Van91I CCANNNNNTGG 1 cut(s) 241
Vha464I CTTAAG 1 cut(s) 467
VpaK11BI GGWCC 2 cut(s) 114, 559
XapI RAATTY 2 cut(s) 195, 215
XhoI CTCGAG 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.