Rorug04G0421300

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016662
Physical Location & Seq
Reverse (-)
58132018 .. 58133859
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0421300.1

Sequence Viewer

Length: 768 bp
ATGAACACCTTTGTTTCGGGAACCATCATCTCAGCCCTTTTCCGTTTCAGGTCGCGTTGTGCCCCGAAAGTTTACAGGGTGAATTGGCATCCACCCATTATTGGTTGGTTCAAAGTTAACACGGATGGTGCTTGGAAGCGAGGGGAGGATCGTTCAGGTTATGGTGGAGTTTTTTCATCATCCCTTGACAGTCCTAGTTCCATTGCTGCGGAGGTTATGGCAGTGATCAAGGCAATCGAGCTTGCATGGGTTCGTGATTGGAAACATATTTGGCTTGAAGTTGACTCGTCCCTAATTCTGAATTTTCTCCGCTCTCCTCAAATGGAAACCAGATGGCTGATGCATTGGTCAATTTTGGCGCTTCAGGCAGGATGTTGTCTGTCATTCCTCATTCGTAGTACTTTACATCTAGGAGGTGTAGGATATGTCCTCTCCTCTTACTTAACCTGCATCACAGTGGCCGTGACTGAGAAATGCGATGTTTATGGTTTTGGTGTGGCGGCCCTAGAAATAATCATGGGAAGACATCCTGGAGAACTGATTTTCTTGACTGCAACATCTTCCTTGATGTCGACAAAGTCACCCTTGGTTTTACAAATTATGCTGAAAGATATGTTGGATCCACGACTATCTCCTCCCAGCATTCAACCGGTAGCTTCTACTGTGGTTATGCTAGCCACATTAGCCTTGGCATGCCTACGCTCCAAACCAAAATGTAGGCCGATAATGGAACATTTTTCAAAGGAGCTTCTGAATCGTACACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

255

Amino Acids

28.39

Weight (kDa)

9.26

Isoelectric Point (pI)

55.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 42 - 111 8.3e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61320 AT1G61330
malus_domestica MD10G1303300.v1.1
prunus_persica Prupe.4G036700_v2.0.a1
pyrus_communis pycom10g25580
rosa_laevigata RLG00000019732
rosa_roxburghii Rroxscaffold_1G00069650 Rroxscaffold_1G00069660
rosa_rugosa Rorug04G0421300
rosa_samantha Rh2BG409000 Rh5BG048300 Rh5BG427600 Rh5DG047400 Rh5DG047500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 572
AccII CGCG 1 cut(s) 55
AciI CCGC 3 cut(s) 209, 310, 500
AclWI GGATC 3 cut(s) 156, 614, 627
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 347
AfaI GTAC 2 cut(s) 400, 760
AfiI CCNNNNNNNGG 1 cut(s) 101
AgeI ACCGGT 1 cut(s) 649
AgsI TTSAA 4 cut(s) 112, 278, 647, 741
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 2 cut(s) 599, 631
AluBI AGCT 3 cut(s) 241, 656, 748
AluI AGCT 3 cut(s) 241, 656, 748
AlwI GGATC 3 cut(s) 156, 614, 627
AoxI GGCC 3 cut(s) 459, 501, 719
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 1 cut(s) 301
AsiGI ACCGGT 1 cut(s) 649
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 1 cut(s) 502
AsuHPI GGTGA 2 cut(s) 91, 573
AsuNHI GCTAGC 1 cut(s) 673
BaeGI GKGCMC 1 cut(s) 64
BamHI GGATCC 1 cut(s) 619
BbsI GAAGAC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 193
BccI CCATC 3 cut(s) 32, 119, 327
BceAI ACGGC 1 cut(s) 446
BciT130I CCWGG 1 cut(s) 531
BclI TGATCA 1 cut(s) 225
BfaI CTAG 4 cut(s) 195, 410, 506, 674
BfoI RGCGCY 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 2 cut(s) 207, 501
BlsI GCNGC 2 cut(s) 208, 502
BmcAI AGTACT 1 cut(s) 400
Bme1390I CCNGG 1 cut(s) 531
BmgT120I GGNCC 1 cut(s) 502
BmiI GGNNCC 2 cut(s) 22, 621
BmrFI CCNGG 1 cut(s) 531
BmsI GCATC 3 cut(s) 97, 330, 459
BmtI GCTAGC 1 cut(s) 677
BpiI GAAGAC 1 cut(s) 529
BpmI CTGGAG 1 cut(s) 552
BsaJI CCNNGG 2 cut(s) 585, 687
BsaWI WCCGGW 1 cut(s) 649
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse118I RCCGGY 1 cut(s) 649
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 585, 687
BseGI GGATG 5 cut(s) 88, 130, 179, 377, 526
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 2 cut(s) 45, 459
BseRI GAGGAG 3 cut(s) 306, 424, 624
BseSI GKGCMC 1 cut(s) 64
BseXI GCAGC 1 cut(s) 193
BseYI CCCAGC 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 3 cut(s) 461, 503, 721
BshTI ACCGGT 1 cut(s) 649
BsiSI CCGG 1 cut(s) 650
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 3 cut(s) 461, 503, 721
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 3 cut(s) 148, 225, 619
BspACI CCGC 3 cut(s) 209, 310, 500
BspANI GGCC 3 cut(s) 461, 503, 721
BspCNI CTCAG 2 cut(s) 44, 460
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 2 cut(s) 22, 621
BspMI ACCTGC 1 cut(s) 455
BspOI GCTAGC 1 cut(s) 677
BspPI GGATC 3 cut(s) 156, 614, 627
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 649
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 2 cut(s) 585, 687
BssMI GATC 3 cut(s) 148, 225, 619
BssT1I CCWWGG 2 cut(s) 585, 687
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 3 cut(s) 191, 457, 664
BstC8I GCNNGC 3 cut(s) 243, 675, 694
BstDEI CTNAG 2 cut(s) 31, 468
BstF5I GGATG 5 cut(s) 88, 130, 179, 377, 526
BstFNI CGCG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 3 cut(s) 151, 228, 622
BstMBI GATC 3 cut(s) 148, 225, 619
BstMWI GCNNNNNNNGC 2 cut(s) 365, 683
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 1 cut(s) 529
BstSLI GKGCMC 1 cut(s) 64
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
BsuRI GGCC 3 cut(s) 461, 503, 721
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 5 cut(s) 88, 130, 179, 377, 526
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 228, 462
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 3 cut(s) 243, 675, 694
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 502
Csp6I GTAC 2 cut(s) 399, 759
CspAI ACCGGT 1 cut(s) 649
CviAII CATG 3 cut(s) 246, 517, 693
CviQI GTAC 2 cut(s) 399, 759
DdeI CTNAG 2 cut(s) 31, 468
DpnI GATC 3 cut(s) 150, 227, 621
DpnII GATC 3 cut(s) 148, 225, 619
EaeI YGGCCR 1 cut(s) 459
Eco130I CCWWGG 2 cut(s) 585, 687
Eco57I CTGAAG 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 529
EcoT14I CCWWGG 2 cut(s) 585, 687
EcoT22I ATGCAT 1 cut(s) 345
ErhI CCWWGG 2 cut(s) 585, 687
FaeI CATG 3 cut(s) 249, 520, 696
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 1 cut(s) 274
FatI CATG 3 cut(s) 245, 516, 692
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 2 cut(s) 207, 501
FokI GGATG 5 cut(s) 75, 137, 166, 384, 513
Fsp4HI GCNGC 2 cut(s) 207, 501
FspBI CTAG 4 cut(s) 195, 410, 506, 674
GlaI GCGC 1 cut(s) 360
GluI GCNGC 2 cut(s) 207, 501
GsaI CCCAGC 1 cut(s) 642
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 3 cut(s) 461, 503, 721
HapII CCGG 1 cut(s) 650
HhaI GCGC 1 cut(s) 361
Hin1II CATG 3 cut(s) 249, 520, 696
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 3 cut(s) 118, 283, 573
HindII GTYRAC 3 cut(s) 118, 283, 573
HinfI GANTC 2 cut(s) 284, 754
HpaI GTTAAC 1 cut(s) 118
HpaII CCGG 1 cut(s) 650
HphI GGTGA 2 cut(s) 91, 573
Hpy166II GTNNAC 5 cut(s) 73, 118, 283, 573, 761
Hpy188I TCNGA 2 cut(s) 300, 753
Hpy188III TCNNGA 3 cut(s) 18, 254, 547
Hpy8I GTNNAC 5 cut(s) 73, 118, 283, 573, 761
HpyCH4III ACNGT 3 cut(s) 191, 457, 664
HpyCH4V TGCA 4 cut(s) 245, 343, 450, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 683
HpyF3I CTNAG 2 cut(s) 31, 468
Hsp92II CATG 3 cut(s) 249, 520, 696
HspAI GCGC 1 cut(s) 359
Ksp22I TGATCA 1 cut(s) 225
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 3 cut(s) 148, 225, 619
LmnI GCTCC 2 cut(s) 707, 745
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 3 cut(s) 97, 330, 459
MaeI CTAG 4 cut(s) 195, 410, 506, 674
MaeIII GTNAC 2 cut(s) 463, 579
MalI GATC 3 cut(s) 150, 227, 621
MbiI CCGCTC 1 cut(s) 312
MboI GATC 3 cut(s) 148, 225, 619
MboII GAAGA 2 cut(s) 534, 552
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 5 cut(s) 82, 294, 301, 351, 597
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 597
MnlI CCTC 9 cut(s) 134, 139, 205, 327, 398, 407, 440, 445, 645
Mph1103I ATGCAT 1 cut(s) 345
MseI TTAA 2 cut(s) 117, 443
MslI CAYNNNNRTG 1 cut(s) 455
MspI CCGG 1 cut(s) 650
MspR9I CCNGG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 642
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 365, 683
NdeII GATC 3 cut(s) 148, 225, 619
NheI GCTAGC 1 cut(s) 673
NlaIII CATG 3 cut(s) 249, 520, 696
NlaIV GGNNCC 2 cut(s) 22, 621
NmuCI GTSAC 2 cut(s) 463, 579
NsiI ATGCAT 1 cut(s) 345
NspI RCATGY 1 cut(s) 696
PaeI GCATGC 1 cut(s) 696
PctI GAATGC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 754
PflFI GACNNNGTC 1 cut(s) 577
PfoI TCCNGGA 1 cut(s) 529
PinAI ACCGGT 1 cut(s) 649
PkrI GCNGC 2 cut(s) 208, 502
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 2 cut(s) 22, 621
PspPI GGNCC 1 cut(s) 502
PsuI RGATCY 1 cut(s) 619
PsyI GACNNNGTC 1 cut(s) 577
RsaI GTAC 2 cut(s) 400, 760
RsaNI GTAC 2 cut(s) 399, 759
RseI CAYNNNNRTG 1 cut(s) 455
SalI GTCGAC 1 cut(s) 571
SaqAI TTAA 2 cut(s) 117, 443
SatI GCNGC 2 cut(s) 207, 501
Sau3AI GATC 3 cut(s) 148, 225, 619
Sau96I GGNCC 1 cut(s) 502
ScaI AGTACT 1 cut(s) 400
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 531
SduI GDGCHC 1 cut(s) 64
SfaNI GCATC 3 cut(s) 97, 330, 459
SmiMI CAYNNNNRTG 1 cut(s) 455
SphI GCATGC 1 cut(s) 696
Sse9I AATT 5 cut(s) 82, 294, 301, 351, 597
SsiI CCGC 3 cut(s) 209, 310, 500
SspMI CTAG 4 cut(s) 195, 410, 506, 674
StyD4I CCNGG 1 cut(s) 529
StyI CCWWGG 2 cut(s) 585, 687
TaaI ACNGT 3 cut(s) 191, 457, 664
TaqI TCGA 2 cut(s) 237, 572
TasI AATT 5 cut(s) 82, 294, 301, 351, 597
TatI WGTACW 1 cut(s) 398
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 1 cut(s) 754
Tru1I TTAA 2 cut(s) 117, 443
Tru9I TTAA 2 cut(s) 117, 443
TscAI CASTG 2 cut(s) 228, 462
TseFI GTSAC 2 cut(s) 463, 579
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 2 cut(s) 463, 579
TspDTI ATGAA 2 cut(s) 17, 165
TspGWI ACGGA 2 cut(s) 32, 137
TspRI CASTG 2 cut(s) 228, 462
Tth111I GACNNNGTC 1 cut(s) 577
XapI RAATTY 1 cut(s) 301
XceI RCATGY 1 cut(s) 696
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmiI GTMKAC 1 cut(s) 572
XspI CTAG 4 cut(s) 195, 410, 506, 674
ZrmI AGTACT 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.