Rh5BG427600

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016662
Physical Location & Seq
Forward (+)
69203495 .. 69203998
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG427600.1

Sequence Viewer

Length: 504 bp
ATGCCATTGAATCCCACCACAAAAGAGGGAAAGATCAAAACCCTCCAGTTATGTTTCGATCCGTCCGATGTTGAGTTTCTTCTTGAGAAGTGGGTTCGGAAATCGGTCAAGAAAGGAATCGAGGAGCTCGATCTCGACATCTTTCAGGAGACTGTGCCGTTTTGCATCCCTACTAATGTTGAAGAGGCAAAGCTAAAAGAGCTTAAGCTCTGTTATTCGGTGATCCATATGCTATTTAATTTGAAGGCTCTGGTATCTCTAACCACCTTGGTTTTGAGGCAGGTTGATCTGACAGACCTGATCATTGAGGTTGTGTTCAAGAACTGTGTCCATAATCGATGGGTCTCAGATTATGGGCTGGAAGCACAGACAAAGATGAAGATGAAGCTCAGATTCAGACCAAATATTGCGATCCTGGGGAAGGGTTTCGATGCAGCATTGAAGAGGGAAGAAGTATCAATGATAGTCCTGTCATCACCAAACATGAGTCAAGTACTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

167

Amino Acids

19.17

Weight (kDa)

7.63

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 12 - 110 4e-08 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61320 AT1G61330
malus_domestica MD10G1303300.v1.1
prunus_persica Prupe.4G036700_v2.0.a1
pyrus_communis pycom10g25580
rosa_laevigata RLG00000019732
rosa_roxburghii Rroxscaffold_1G00069650 Rroxscaffold_1G00069660
rosa_rugosa Rorug04G0421300
rosa_samantha Rh2BG409000 Rh5BG048300 Rh5BG427600 Rh5DG047400 Rh5DG047500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AclWI GGATC 3 cut(s) 53, 217, 406
AfaI GTAC 1 cut(s) 495
AfiI CCNNNNNNNGG 1 cut(s) 421
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 5 cut(s) 10, 182, 244, 319, 442
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 5 cut(s) 127, 193, 202, 208, 388
AluI AGCT 5 cut(s) 127, 193, 202, 208, 388
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 143, 349
AlwI GGATC 3 cut(s) 53, 217, 406
ApeKI GCWGC 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 232, 468
BanII GRGCYC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 446
BccI CCATC 1 cut(s) 333
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 1 cut(s) 416
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 143, 349
BfaI CTAG 1 cut(s) 497
BfrI CTTAAG 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
BmcAI AGTACT 1 cut(s) 495
Bme1390I CCNGG 1 cut(s) 416
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 2 cut(s) 174, 421
BpmI CTGGAG 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 104
Bsa29I ATCGAT 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 2 cut(s) 267, 415
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 46
BseBI CCWGG 1 cut(s) 416
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 267, 415
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMII CTCAG 2 cut(s) 360, 403
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 446
BshVI ATCGAT 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 129
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 2 cut(s) 143, 349
Bso31I GGTCTC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
BspCNI CTCAG 2 cut(s) 359, 402
BspDI ATCGAT 1 cut(s) 337
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 3 cut(s) 53, 217, 406
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 349
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 267, 415
BssMI GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 2 cut(s) 154, 326
Bst6I CTCTTC 2 cut(s) 177, 437
BstAFI CTTAAG 1 cut(s) 203
BstDEI CTNAG 2 cut(s) 346, 389
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 165
BstKTI GATC 7 cut(s) 36, 61, 133, 225, 289, 303, 414
BstMAI GTCTC 2 cut(s) 143, 349
BstMBI GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
BstMWI GCNNNNNNNGC 1 cut(s) 199
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 337
BsuTUI ATCGAT 1 cut(s) 337
BtsCI GGATG 1 cut(s) 165
BveI ACCTGC 1 cut(s) 271
ClaI ATCGAT 1 cut(s) 337
Csp6I GTAC 1 cut(s) 494
CviAII CATG 1 cut(s) 484
CviJI RGCY 7 cut(s) 127, 193, 202, 208, 248, 358, 388
CviKI_1 RGCY 7 cut(s) 127, 193, 202, 208, 248, 358, 388
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 2 cut(s) 346, 389
DpnI GATC 7 cut(s) 35, 60, 132, 224, 288, 302, 413
DpnII GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
Eam1104I CTCTTC 2 cut(s) 177, 437
EarI CTCTTC 2 cut(s) 177, 437
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 127
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 1 cut(s) 487
FaiI YATR 6 cut(s) 52, 228, 230, 333, 354, 485
FatI CATG 1 cut(s) 483
FauNDI CATATG 1 cut(s) 228
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 1 cut(s) 497
GluI GCNGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 29
Hin1II CATG 1 cut(s) 487
HinfI GANTC 4 cut(s) 10, 117, 393, 487
HphI GGTGA 2 cut(s) 232, 468
Hpy188I TCNGA 6 cut(s) 67, 99, 291, 349, 392, 398
Hpy188III TCNNGA 5 cut(s) 83, 109, 134, 146, 319
HpyAV CCTTC 2 cut(s) 238, 415
HpyCH4III ACNGT 2 cut(s) 154, 326
HpyCH4V TGCA 2 cut(s) 165, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 199
HpyF3I CTNAG 2 cut(s) 346, 389
Hsp92II CATG 1 cut(s) 487
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 9 cut(s) 59, 131, 236, 266, 311, 344, 401, 428, 482
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 2 cut(s) 174, 421
MaeI CTAG 1 cut(s) 497
MalI GATC 7 cut(s) 35, 60, 132, 224, 288, 302, 413
MboI GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
MboII GAAGA 5 cut(s) 71, 194, 391, 454, 461
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 1 cut(s) 238
MlyI GAGTC 1 cut(s) 496
MnlI CCTC 7 cut(s) 19, 53, 115, 178, 270, 301, 438
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 2 cut(s) 204, 237
MspCI CTTAAG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 199
NdeI CATATG 1 cut(s) 228
NdeII GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
NlaIII CATG 1 cut(s) 487
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 126
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 3 cut(s) 10, 117, 393
PkrI GCNGC 1 cut(s) 436
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 2 cut(s) 204, 237
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 7 cut(s) 33, 58, 130, 222, 286, 300, 411
ScaI AGTACT 1 cut(s) 495
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 129
SetI ASST 9 cut(s) 129, 195, 204, 210, 269, 285, 300, 312, 390
SfaNI GCATC 2 cut(s) 174, 421
SmlI CTYRAG 2 cut(s) 83, 203
SmoI CTYRAG 2 cut(s) 83, 203
Sse9I AATT 1 cut(s) 238
SspI AATATT 1 cut(s) 406
SspMI CTAG 1 cut(s) 497
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 154, 326
TaqI TCGA 6 cut(s) 57, 120, 129, 135, 337, 429
TaqII GACCGA 1 cut(s) 94
TasI AATT 1 cut(s) 238
TatI WGTACW 1 cut(s) 493
TfiI GAWTC 3 cut(s) 10, 117, 393
Tru1I TTAA 2 cut(s) 204, 237
Tru9I TTAA 2 cut(s) 204, 237
TseI GCWGC 1 cut(s) 434
TspDTI ATGAA 2 cut(s) 392, 398
TspGWI ACGGA 1 cut(s) 51
Vha464I CTTAAG 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 497
ZrmI AGTACT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.