RLG00000019732

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016662
Physical Location & Seq
Reverse (-)
56083652 .. 56085440
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019732

Sequence Viewer

Length: 1455 bp
ATGGCTTCATCTTCTGCAGAGTCATCGAGAAGAGGAGCAAAGAGGCAACGCCAAAACAACCGCAACCATCATGATGATTCCTGGGTGTTTGATGTTCACAGTGATCTCGTGGAGGAAATCTTCACTCACCTGCCAATACGGCAGTGCGTGCAGATAGGGCTGGTGGCCAAGGAGTTTCGAGGAACATGGCTGAAATGCCGAAATCTCGACTTTGGGAGAGAATTCGCAGCCACCCAACCCCACCGGTTGGCTTACTGCACTGCCGTGGAACATGCCATTGAATCCCACCACAAAGAGGGAAAGATCAAAACCCTCCGGCTATGTTTCGATCCGTCCGGTGTTGAGTTTCTTCTGGAGAAGTGGGTTCGGAAATCAGTCAAGAAAGGAGTCGAGGAGCTCGATCTCGACATCTTTCAGGCGACGGTGCCGTTTTGCTTCCCTACTAATGCTGAAGAGGCAAAGCTGAAAGAGCTCAAGCTCTATTATTCGGTGATCCAGATGCCGTTTAATCTGAAGGCTCTGGTATCTCTGACCACCTTGGTTCTGAGGCAGGTTGATCTGACGGACCTGATCATTGAGGCTCTGTTCGAGAACTGTGTCCATCTCGAGACCTTTGACATGGCTGATTGCTATGGAGTAAGAAATATGAAGGTGAAGGCGAACAACAGATTTAAGGTGTTAATGGTTGGAGGGTGCCCGAAGATATCGAGCATCAAAATTGATGCCCCAAGGCTTCGATGTCTGTACTACAATGGGGTGGTAAAACGATTCAACATATCTGAGCCTTTTTTGTTCTTGAACGAGGTCATTTTGAATTTCTTGCCCTCAAAAGGGTACACCAAACCTTGGCTTGTTGAGAAGCTTGTTGCTGATACCTCCAGTGTGAGAGTGCTCACCATTACTAGCACATTTCTTGAGGGCTTGGCTGCCAAGATTCGCCGCGGATGTATTCGAGAGATGCAGTTTTGCTTCTGGAACTTGAGGGAGCTCCAAATAGTCATGCAGGATCCAAGCTTCACTAATGCCGGAGATGTTGCGTCTTTTATCAAAAACTGTCCAAGGCTGGAGAATTTGTTTATTGAGCTGAATGAGAATACTTATGAGAGCGCAATTTACCGTGATATGCTACAAAATCCAGCAATGGATCACAATATGAGCTTCCTCAATGCCCTCAAATTTGTCAAAATAAGAGGGTTTAAGGTTACAAATAAGCAACACGAACAGTTAGCAAGGTTCATCTTAGACAACGCGAGGTTCTTAGAGCGTATGGTCCTCCTATGGCCTACGAACCGATACCATTTATGCCGCAGTGTGCCTTATCTACCTCTCAGTGAACAGATCAGGTACGCCAACATCCTTGCATGTTGGCAACTGTCTGCCAGAGCGGAAATTATATTGTGTGACCAAATGGAGGACATGTATGGATTCCCGAAACATGTAAGGAAATGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

485

Amino Acids

56.3

Weight (kDa)

8.91

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 97 - 425 1.5e-29 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 118 - 250 2.3e-07 FBD-associated F-box protein At5g56370, LRR repeats
FBD PF08387 387 - 425 1.6e-06 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61320 AT1G61330
malus_domestica MD10G1303300.v1.1
prunus_persica Prupe.4G036700_v2.0.a1
pyrus_communis pycom10g25580
rosa_laevigata RLG00000019732
rosa_roxburghii Rroxscaffold_1G00069650 Rroxscaffold_1G00069660
rosa_rugosa Rorug04G0421300
rosa_samantha Rh2BG409000 Rh5BG048300 Rh5BG427600 Rh5DG047400 Rh5DG047500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 138
Acc36I ACCTGC 2 cut(s) 138, 541
AccB1I GGYRCC 2 cut(s) 424, 693
AccB7I CCANNNNNTGG 2 cut(s) 247, 846
AccBSI CCGCTC 1 cut(s) 1385
AccII CGCG 2 cut(s) 942, 1250
AciI CCGC 5 cut(s) 61, 940, 942, 1306, 1385
AclWI GGATC 5 cut(s) 323, 487, 1001, 1014, 1152
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 4 cut(s) 221, 814, 1069, 1175
AcuI CTGAAG 2 cut(s) 471, 533
AfaI GTAC 3 cut(s) 746, 836, 1346
AfiI CCNNNNNNNGG 6 cut(s) 247, 295, 830, 831, 846, 1411
AflIII ACRYGT 2 cut(s) 1416, 1435
AgeI ACCGGT 1 cut(s) 243
AgsI TTSAA 4 cut(s) 281, 772, 799, 814
AjnI CCWGG 1 cut(s) 80
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 9 cut(s) 397, 463, 472, 478, 862, 988, 1014, 1084, 1158
AluI AGCT 9 cut(s) 397, 463, 472, 478, 862, 988, 1014, 1084, 1158
Alw21I GWGCWC 4 cut(s) 399, 474, 894, 990
Alw26I GTCTC 1 cut(s) 602
AlwI GGATC 5 cut(s) 323, 487, 1001, 1014, 1152
Ama87I CYCGRG 1 cut(s) 605
AoxI GGCC 2 cut(s) 165, 1280
ApeKI GCWGC 2 cut(s) 227, 926
ApoI RAATTY 4 cut(s) 221, 814, 1069, 1175
AsiGI ACCGGT 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 1448
AspLEI GCGC 1 cut(s) 1109
AspS9I GGNCC 2 cut(s) 565, 1270
AsuHPI GGTGA 4 cut(s) 119, 502, 664, 886
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 2 cut(s) 565, 1270
BaeGI GKGCMC 1 cut(s) 698
BalI TGGCCA 1 cut(s) 167
BamHI GGATCC 1 cut(s) 1006
BanI GGYRCC 2 cut(s) 424, 693
BanII GRGCYC 3 cut(s) 399, 474, 990
BauI CACGAG 1 cut(s) 107
Bbv12I GWGCWC 4 cut(s) 399, 474, 894, 990
BbvI GCAGC 2 cut(s) 239, 913
BccI CCATC 2 cut(s) 75, 609
BceAI ACGGC 4 cut(s) 155, 248, 412, 487
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 82
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 2 cut(s) 903, 1453
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 2 cut(s) 138, 541
BglI GCCNNNNNGGC 1 cut(s) 139
BisI GCNGC 4 cut(s) 228, 927, 940, 1306
BlsI GCNGC 4 cut(s) 229, 928, 941, 1307
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 2 cut(s) 565, 1270
BmeT110I CYCGRG 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 565, 1270
BmiI GGNNCC 3 cut(s) 426, 695, 1008
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 4 cut(s) 489, 712, 720, 948
BpmI CTGGAG 3 cut(s) 374, 862, 1085
BpuEI CTTGAG 3 cut(s) 458, 935, 1000
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 8 cut(s) 81, 168, 264, 537, 728, 845, 940, 1058
BsaWI WCCGGW 2 cut(s) 243, 335
BsaXI ACNNNNNCTCC 4 cut(s) 1058, 1088, 1403, 1433
Bsc4I CCNNNNNNNGG 6 cut(s) 247, 295, 830, 831, 846, 1411
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 879
Bse3DI GCAATG 1 cut(s) 1146
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 8 cut(s) 81, 168, 264, 537, 728, 845, 940, 1058
BseGI GGATG 2 cut(s) 950, 1353
BseLI CCNNNNNNNGG 6 cut(s) 247, 295, 830, 831, 846, 1411
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 3 cut(s) 536, 771, 1342
BseNI ACTGG 1 cut(s) 879
BseRI GAGGAG 2 cut(s) 48, 407
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 2 cut(s) 239, 913
BsgI GTGCAG 2 cut(s) 170, 241
Bsh1236I CGCG 2 cut(s) 942, 1250
BshFI GGCC 2 cut(s) 167, 1282
BshNI GGYRCC 2 cut(s) 424, 693
BshTI ACCGGT 1 cut(s) 243
BsiHKAI GWGCWC 4 cut(s) 399, 474, 894, 990
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 4 cut(s) 244, 316, 336, 1026
BslI CCNNNNNNNGG 6 cut(s) 247, 295, 830, 831, 846, 1411
BsmAI GTCTC 1 cut(s) 602
BsnI GGCC 2 cut(s) 167, 1282
Bso31I GGTCTC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 605
Bsp1286I GDGCHC 5 cut(s) 399, 474, 698, 894, 990
BspACI CCGC 5 cut(s) 61, 940, 942, 1306, 1385
BspANI GGCC 2 cut(s) 167, 1282
BspCNI CTCAG 3 cut(s) 537, 772, 1341
BspFNI CGCG 2 cut(s) 942, 1250
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 3 cut(s) 426, 695, 1008
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 2 cut(s) 138, 541
BspPI GGATC 5 cut(s) 323, 487, 1001, 1014, 1152
BspT107I GGYRCC 2 cut(s) 424, 693
BspTNI GGTCTC 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 1385
BsrDI GCAATG 1 cut(s) 1146
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 879
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 8 cut(s) 81, 168, 264, 537, 728, 845, 940, 1058
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 5 cut(s) 168, 537, 728, 845, 1058
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 7 cut(s) 101, 424, 596, 1055, 1118, 1224, 1374
Bst6I CTCTTC 2 cut(s) 25, 447
BstAPI GCANNNNNTGC 1 cut(s) 148
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 5 cut(s) 545, 780, 1240, 1258, 1328
BstDSI CCRYGG 2 cut(s) 264, 940
BstF5I GGATG 2 cut(s) 950, 1353
BstFNI CGCG 2 cut(s) 942, 1250
BstHHI GCGC 1 cut(s) 1109
BstMAI GTCTC 1 cut(s) 602
BstMWI GCNNNNNNNGC 5 cut(s) 139, 148, 157, 455, 469
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 4 cut(s) 275, 1365, 1420, 1439
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 698
BstUI CGCG 2 cut(s) 942, 1250
BstV1I GCAGC 2 cut(s) 239, 913
BstX2I RGATCY 1 cut(s) 1006
BstYI RGATCY 1 cut(s) 1006
BsuRI GGCC 2 cut(s) 167, 1282
BtgI CCRYGG 2 cut(s) 264, 940
BtsCI GGATG 2 cut(s) 950, 1353
BtsI GCAGTG 3 cut(s) 149, 258, 1315
BtsIMutI CAGTG 6 cut(s) 106, 149, 258, 886, 1315, 1336
BveI ACCTGC 2 cut(s) 138, 541
Cac8I GCNNGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 70
CfoI GCGC 1 cut(s) 1109
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 565, 1270
Cfr42I CCGCGG 1 cut(s) 943
CseI GACGC 1 cut(s) 1026
Csp6I GTAC 3 cut(s) 745, 835, 1345
CspAI ACCGGT 1 cut(s) 243
CviAII CATG 8 cut(s) 71, 186, 272, 619, 1000, 1362, 1417, 1436
CviQI GTAC 3 cut(s) 745, 835, 1345
DdeI CTNAG 5 cut(s) 545, 780, 1240, 1258, 1328
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 2 cut(s) 25, 447
EarI CTCTTC 2 cut(s) 25, 447
Ecl136II GAGCTC 3 cut(s) 397, 472, 988
Eco130I CCWWGG 5 cut(s) 168, 537, 728, 845, 1058
Eco24I GRGCYC 3 cut(s) 399, 474, 990
Eco31I GGTCTC 1 cut(s) 602
Eco32I GATATC 1 cut(s) 705
Eco47I GGWCC 2 cut(s) 565, 1270
Eco53kI GAGCTC 3 cut(s) 397, 472, 988
Eco57I CTGAAG 2 cut(s) 471, 533
Eco88I CYCGRG 1 cut(s) 605
EcoICRI GAGCTC 3 cut(s) 397, 472, 988
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 80
EcoRV GATATC 1 cut(s) 705
EcoT14I CCWWGG 5 cut(s) 168, 537, 728, 845, 1058
EcoT38I GRGCYC 3 cut(s) 399, 474, 990
ErhI CCWWGG 5 cut(s) 168, 537, 728, 845, 1058
FaeI CATG 8 cut(s) 74, 189, 275, 622, 1003, 1365, 1420, 1439
FatI CATG 8 cut(s) 70, 185, 271, 618, 999, 1361, 1416, 1435
FbaI TGATCA 1 cut(s) 570
Fnu4HI GCNGC 4 cut(s) 228, 927, 940, 1306
FokI GGATG 2 cut(s) 957, 1340
FriOI GRGCYC 3 cut(s) 399, 474, 990
Fsp4HI GCNGC 4 cut(s) 228, 927, 940, 1306
FspBI CTAG 2 cut(s) 903, 1453
GlaI GCGC 1 cut(s) 1108
GluI GCNGC 4 cut(s) 228, 927, 940, 1306
GsuI CTGGAG 3 cut(s) 374, 862, 1085
HaeIII GGCC 2 cut(s) 167, 1282
HapII CCGG 4 cut(s) 244, 316, 336, 1026
HgaI GACGC 1 cut(s) 1026
HhaI GCGC 1 cut(s) 1109
Hin1II CATG 8 cut(s) 74, 189, 275, 622, 1003, 1365, 1420, 1439
Hin6I GCGC 1 cut(s) 1107
HinP1I GCGC 1 cut(s) 1107
HindIII AAGCTT 2 cut(s) 860, 1012
HinfI GANTC 7 cut(s) 20, 77, 281, 387, 768, 934, 1425
HpaII CCGG 4 cut(s) 244, 316, 336, 1026
HphI GGTGA 4 cut(s) 119, 502, 664, 886
Hpy166II GTNNAC 3 cut(s) 97, 837, 1334
Hpy188I TCNGA 6 cut(s) 369, 513, 531, 546, 561, 781
Hpy8I GTNNAC 3 cut(s) 97, 837, 1334
Hpy99I CGWCG 1 cut(s) 424
HpyAV CCTTC 3 cut(s) 508, 643, 649
HpyCH4III ACNGT 7 cut(s) 101, 424, 596, 1055, 1118, 1224, 1374
HpyCH4V TGCA 6 cut(s) 17, 151, 258, 961, 1003, 1361
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 148, 157, 455, 469
HpyF3I CTNAG 5 cut(s) 545, 780, 1240, 1258, 1328
Hsp92II CATG 8 cut(s) 74, 189, 275, 622, 1003, 1365, 1420, 1439
HspAI GCGC 1 cut(s) 1107
Ksp22I TGATCA 1 cut(s) 570
KspI CCGCGG 1 cut(s) 943
LmnI GCTCC 4 cut(s) 35, 394, 985, 993
Lsp1109I GCAGC 2 cut(s) 239, 913
LweI GCATC 4 cut(s) 489, 712, 720, 948
MaeI CTAG 2 cut(s) 903, 1453
MaeIII GTNAC 2 cut(s) 1201, 1400
MbiI CCGCTC 1 cut(s) 1385
MboII GAAGA 6 cut(s) 3, 42, 112, 341, 464, 712
MflI RGATCY 1 cut(s) 1006
MhlI GDGCHC 5 cut(s) 399, 474, 698, 894, 990
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 7 cut(s) 221, 717, 814, 1069, 1110, 1175, 1389
MluNI TGGCCA 1 cut(s) 167
MlyI GAGTC 2 cut(s) 29, 396
MmeI TCCRAC 1 cut(s) 667
Mox20I TGGCCA 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 1448
MscI TGGCCA 1 cut(s) 167
MseI TTAA 4 cut(s) 507, 672, 680, 1197
MslI CAYNNNNRTG 2 cut(s) 72, 263
Msp20I TGGCCA 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 942
MspI CCGG 4 cut(s) 244, 316, 336, 1026
MspR9I CCNGG 1 cut(s) 82
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 2 cut(s) 942, 1250
MwoI GCNNNNNNNGC 5 cut(s) 139, 148, 157, 455, 469
NlaIII CATG 8 cut(s) 74, 189, 275, 622, 1003, 1365, 1420, 1439
NlaIV GGNNCC 3 cut(s) 426, 695, 1008
NmuCI GTSAC 1 cut(s) 1400
NspI RCATGY 4 cut(s) 275, 1365, 1420, 1439
OliI CACNNNNGTG 1 cut(s) 263
PaeR7I CTCGAG 1 cut(s) 605
PagI TCATGA 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 138
PciI ACATGT 2 cut(s) 1416, 1435
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PdmI GAANNNNTTC 1 cut(s) 1448
PfeI GAWTC 5 cut(s) 77, 281, 768, 934, 1425
PflMI CCANNNNNTGG 2 cut(s) 247, 846
PinAI ACCGGT 1 cut(s) 243
PkrI GCNGC 4 cut(s) 229, 928, 941, 1307
PleI GAGTC 2 cut(s) 28, 395
PpsI GAGTC 2 cut(s) 28, 395
PscI ACATGT 2 cut(s) 1416, 1435
Psp124BI GAGCTC 3 cut(s) 399, 474, 990
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 3 cut(s) 426, 695, 1008
PspPI GGNCC 2 cut(s) 565, 1270
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 1006
RsaI GTAC 3 cut(s) 746, 836, 1346
RsaNI GTAC 3 cut(s) 745, 835, 1345
RseI CAYNNNNRTG 2 cut(s) 72, 263
SacI GAGCTC 3 cut(s) 399, 474, 990
SacII CCGCGG 1 cut(s) 943
SaqAI TTAA 4 cut(s) 507, 672, 680, 1197
SatI GCNGC 4 cut(s) 228, 927, 940, 1306
Sau96I GGNCC 2 cut(s) 565, 1270
SchI GAGTC 2 cut(s) 29, 396
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 5 cut(s) 399, 474, 698, 894, 990
SfaNI GCATC 4 cut(s) 489, 712, 720, 948
SfcI CTRYAG 1 cut(s) 15
Sfr274I CTCGAG 1 cut(s) 605
Sfr303I CCGCGG 1 cut(s) 943
SgrBI CCGCGG 1 cut(s) 943
SinI GGWCC 2 cut(s) 565, 1270
SlaI CTCGAG 1 cut(s) 605
SmiMI CAYNNNNRTG 2 cut(s) 72, 263
SmlI CTYRAG 4 cut(s) 473, 605, 914, 979
SmoI CTYRAG 4 cut(s) 473, 605, 914, 979
Sse9I AATT 7 cut(s) 221, 717, 814, 1069, 1110, 1175, 1389
SsiI CCGC 5 cut(s) 61, 940, 942, 1306, 1385
SspMI CTAG 2 cut(s) 903, 1453
SstI GAGCTC 3 cut(s) 399, 474, 990
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 5 cut(s) 168, 537, 728, 845, 1058
TaaI ACNGT 7 cut(s) 101, 424, 596, 1055, 1118, 1224, 1374
TasI AATT 7 cut(s) 221, 717, 814, 1069, 1110, 1175, 1389
TatI WGTACW 1 cut(s) 744
TauI GCSGC 2 cut(s) 942, 1308
TfiI GAWTC 5 cut(s) 77, 281, 768, 934, 1425
Tru1I TTAA 4 cut(s) 507, 672, 680, 1197
Tru9I TTAA 4 cut(s) 507, 672, 680, 1197
TscAI CASTG 6 cut(s) 106, 149, 265, 886, 1315, 1336
TseFI GTSAC 1 cut(s) 1400
TseI GCWGC 2 cut(s) 227, 926
Tsp45I GTSAC 1 cut(s) 1400
TspDTI ATGAA 2 cut(s) 662, 1225
TspGWI ACGGA 2 cut(s) 321, 578
TspRI CASTG 6 cut(s) 106, 149, 265, 886, 1315, 1336
Van91I CCANNNNNTGG 2 cut(s) 247, 846
VpaK11BI GGWCC 2 cut(s) 565, 1270
XapI RAATTY 4 cut(s) 221, 814, 1069, 1175
XceI RCATGY 4 cut(s) 275, 1365, 1420, 1439
XhoI CTCGAG 1 cut(s) 605
XmnI GAANNNNTTC 1 cut(s) 1448
XspI CTAG 2 cut(s) 903, 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.