Rh5DG047500

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016662
Physical Location & Seq
Reverse (-)
3931839 .. 3964811
32973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG047500.1

Sequence Viewer

Length: 1395 bp
ATGCAGGAGCCATGGCTGCTTCATGACTTTGATGAGGACTTCTATGGGGAGGAATTGCATCTTATCATAGTTGGCTACATACGGCCTGAGGCCAATTTTCCGTCTCTCGAGAGCTTGATCGAAAAGATCCATGAGGACAGGAAGATTGCAGAGGAAGCTCTTGATCTTCCAATTAATGGAGAAGAAGAAGAGCAACAAGAGCAGCAACACCTATCCAATTCGCCCAACCCAGATACCGACATTACCGAAAACCCAAGCCACCAGCAAGAAGAGTACTCATGGCCAGCGATTCGGTTCGATGTTCCTCCACAGAGAACCTTCTCCCAGCAGTTCAGAACTCCCTCAAACCCTAACAATTTTCTCAAGGCCGTCAAATGGTCGCTGGATGGTTCTTGCTTTCTCACCAGTTCTGAGGACAACACCCTCCGTGTTTTCGCAATACCGGAAAGTGGGAATGAATATGTTAACGGCTATTCTGCGCCTGCTGATGAAGTGCCGTTTGTCTTTGACTGTACACTGAAGAAGGCTCTGAAACCTCATAATCGCTGTGATCTTGTGGAGGAAATCTTCACTCACCTGCCGATAAAGCAGTGTGTGCAGATAGGGCTGGTGGCCAAGGAGTTTCGGGAAACATGGAGGAAATGCCGGAATCTCGATTTCGGGAGAGAATTTGCGGCCACCCAACGCCATCGGTTGGCTTACAGCGCCACCGTGGATCATGTCATTAAATCCCACGACAAAGAAGAAAAGATCAAAACCTTCCGGTTATGTTTCGACGCGTCTGGAGTTGAGTTTCTTCTTAAGGAGTGGGTTCGGGAATCAGTCAGGAAAGGAGTCAAGGAGCTCGATCTAGACGTCTTTCAGGCAACGGTGCCATTTTGTGTCCCCACCAATGCTGAAGAGGCAACGCTCAAGGAGCTCAAGTTGTCATACTCGATGATCCAGATGTCGTTTAATTTGAAGGCTCTGGTGTCTCTGACCACCTTGGTTCTGAGGCAAGTTGATCTGACGGCCTTGATCGTCGAGGCCGTGTTTGAAAATTGTGCCCATCTCGAGACCTTTGACATAGCTGACTGCTATGGAGTGAGGAATATGAAGGTGAAGGCGAACAAGAGATTTAAGGAGTTAAAGGTGGGAGGGTGCCTGGAGATATCGAGCCTCGAGATTAGTGCCCCGAGGCTTCGATGTCTCTACTACAATGGGGTGGTGAAACGATTCAACATATATGAGCCTTTGTTGTTCTTGAACGAGGCCATTTTGAATTTTTTGCCCTCAAAAGGGTACACCAACCCTTGGCTTGTTGAAAAGCTTGTTGCTGATGCCTCCACTGTGAGAGTGCTCACCTTTACTAGAACATTTCTTGAGGTAGTGAATGACTTTATCCCAAAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

464

Amino Acids

53.41

Weight (kDa)

5.25

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavokinase PF01687 7 - 53 2e-10 Riboflavin kinase
LRR_At1g61320_AtMIF1 PF23622 250 - 405 7.2e-18 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 267 - 395 2.7e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61320 AT1G61330
malus_domestica MD10G1303300.v1.1
prunus_persica Prupe.4G036700_v2.0.a1
pyrus_communis pycom10g25580
rosa_laevigata RLG00000019732
rosa_roxburghii Rroxscaffold_1G00069650 Rroxscaffold_1G00069660
rosa_rugosa Rorug04G0421300
rosa_samantha Rh2BG409000 Rh5BG048300 Rh5BG427600 Rh5DG047400 Rh5DG047500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 585
AatII GACGTC 1 cut(s) 858
Acc36I ACCTGC 1 cut(s) 585
AccB1I GGYRCC 2 cut(s) 871, 1140
AccB7I CCANNNNNTGG 3 cut(s) 176, 694, 1293
AccII CGCG 1 cut(s) 779
AciI CCGC 1 cut(s) 674
AclWI GGATC 3 cut(s) 121, 723, 934
AcoI YGGCCR 3 cut(s) 281, 612, 675
AcsI RAATTY 2 cut(s) 668, 1261
AcuI CTGAAG 2 cut(s) 539, 918
AcyI GRCGYC 1 cut(s) 855
AfaI GTAC 3 cut(s) 275, 514, 1283
AfiI CCNNNNNNNGG 7 cut(s) 176, 375, 449, 694, 1277, 1278, 1293
AflII CTTAAG 1 cut(s) 800
AflIII ACRYGT 1 cut(s) 777
AgsI TTSAA 6 cut(s) 961, 1037, 1219, 1246, 1261, 1304
AjnI CCWGG 1 cut(s) 1143
AluBI AGCT 6 cut(s) 114, 158, 844, 919, 1070, 1309
AluI AGCT 6 cut(s) 114, 158, 844, 919, 1070, 1309
Alw21I GWGCWC 3 cut(s) 846, 921, 1341
Alw26I GTCTC 4 cut(s) 108, 978, 1049, 1193
AlwI GGATC 3 cut(s) 121, 723, 934
Ama87I CYCGRG 4 cut(s) 107, 1052, 1160, 1174
AoxI GGCC 9 cut(s) 83, 90, 281, 366, 612, 675, 1011, 1026, 1251
ApeKI GCWGC 2 cut(s) 16, 202
ApoI RAATTY 2 cut(s) 668, 1261
AseI ATTAAT 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 1214
AspLEI GCGC 2 cut(s) 481, 707
AsuHPI GGTGA 5 cut(s) 394, 566, 1111, 1219, 1333
AvaI CYCGRG 4 cut(s) 107, 1052, 1160, 1174
AxyI CCTNAGG 1 cut(s) 87
BaeGI GKGCMC 2 cut(s) 1048, 1174
BalI TGGCCA 2 cut(s) 283, 614
BanI GGYRCC 2 cut(s) 871, 1140
BanII GRGCYC 2 cut(s) 846, 921
Bbv12I GWGCWC 3 cut(s) 846, 921, 1341
BbvI GCAGC 2 cut(s) 3, 214
BccI CCATC 3 cut(s) 380, 696, 1056
BceAI ACGGC 6 cut(s) 98, 353, 481, 484, 1013, 1026
BcgI CGANNNNNNTGC 2 cut(s) 1151, 1185
BciT130I CCWGG 1 cut(s) 1145
BcoDI GTCTC 4 cut(s) 108, 978, 1049, 1193
BfaI CTAG 2 cut(s) 851, 1350
BfoI RGCGCY 1 cut(s) 708
BfrI CTTAAG 1 cut(s) 800
BfuAI ACCTGC 1 cut(s) 585
BisI GCNGC 3 cut(s) 17, 203, 675
BlsI GCNGC 3 cut(s) 18, 204, 676
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 1 cut(s) 1145
BmeT110I CYCGRG 4 cut(s) 107, 1052, 1160, 1174
BmiI GGNNCC 3 cut(s) 9, 873, 1142
BmrFI CCNGG 1 cut(s) 1145
BmsI GCATC 2 cut(s) 67, 1309
BpmI CTGGAG 2 cut(s) 804, 1166
BpuEI CTTGAG 4 cut(s) 347, 896, 905, 1382
BsaHI GRCGYC 1 cut(s) 855
BsaI GGTCTC 1 cut(s) 1049
BsaJI CCNNGG 6 cut(s) 11, 615, 711, 984, 1175, 1292
BsaWI WCCGGW 2 cut(s) 442, 762
BsaXI ACNNNNNCTCC 2 cut(s) 908, 938
Bsc4I CCNNNNNNNGG 7 cut(s) 176, 375, 449, 694, 1277, 1278, 1293
Bse1I ACTGG 1 cut(s) 405
Bse21I CCTNAGG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 1145
BseDI CCNNGG 6 cut(s) 11, 615, 711, 984, 1175, 1292
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 7 cut(s) 176, 375, 449, 694, 1277, 1278, 1293
BseMII CTCAG 3 cut(s) 78, 402, 983
BseNI ACTGG 1 cut(s) 405
BseSI GKGCMC 2 cut(s) 1048, 1174
BseXI GCAGC 2 cut(s) 3, 214
BseYI CCCAGC 1 cut(s) 324
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 779
BshFI GGCC 9 cut(s) 85, 92, 283, 368, 614, 677, 1013, 1028, 1253
BshNI GGYRCC 2 cut(s) 871, 1140
BsiHKAI GWGCWC 3 cut(s) 846, 921, 1341
BsiHKCI CYCGRG 4 cut(s) 107, 1052, 1160, 1174
BsiSI CCGG 3 cut(s) 443, 646, 763
BslFI GGGAC 1 cut(s) 869
BslI CCNNNNNNNGG 7 cut(s) 176, 375, 449, 694, 1277, 1278, 1293
BsmAI GTCTC 4 cut(s) 108, 978, 1049, 1193
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 869
BsnI GGCC 9 cut(s) 85, 92, 283, 368, 614, 677, 1013, 1028, 1253
Bso31I GGTCTC 1 cut(s) 1049
BsoBI CYCGRG 4 cut(s) 107, 1052, 1160, 1174
Bsp1286I GDGCHC 5 cut(s) 846, 921, 1048, 1174, 1341
Bsp1407I TGTACA 1 cut(s) 512
Bsp19I CCATGG 1 cut(s) 11
BspACI CCGC 1 cut(s) 674
BspANI GGCC 9 cut(s) 85, 92, 283, 368, 614, 677, 1013, 1028, 1253
BspCNI CTCAG 3 cut(s) 79, 403, 984
BspFNI CGCG 1 cut(s) 779
BspHI TCATGA 1 cut(s) 22
BspLI GGNNCC 3 cut(s) 9, 873, 1142
BspMI ACCTGC 1 cut(s) 585
BspPI GGATC 3 cut(s) 121, 723, 934
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 2 cut(s) 871, 1140
BspTI CTTAAG 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 1049
BsrGI TGTACA 1 cut(s) 512
BsrI ACTGG 1 cut(s) 405
BssECI CCNNGG 6 cut(s) 11, 615, 711, 984, 1175, 1292
BssNI GRCGYC 1 cut(s) 855
BssT1I CCWWGG 4 cut(s) 11, 615, 984, 1292
Bst2UI CCWGG 1 cut(s) 1145
Bst4CI ACNGT 4 cut(s) 512, 712, 871, 1330
Bst6I CTCTTC 3 cut(s) 183, 264, 894
BstACI GRCGYC 1 cut(s) 855
BstAFI CTTAAG 1 cut(s) 800
BstAPI GCANNNNNTGC 1 cut(s) 595
BstAUI TGTACA 1 cut(s) 512
BstC8I GCNNGC 2 cut(s) 285, 483
BstDEI CTNAG 3 cut(s) 87, 411, 992
BstDSI CCRYGG 2 cut(s) 11, 711
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 779
BstH2I RGCGCY 1 cut(s) 708
BstHHI GCGC 2 cut(s) 481, 707
BstMAI GTCTC 4 cut(s) 108, 978, 1049, 1193
BstMWI GCNNNNNNNGC 9 cut(s) 16, 155, 199, 586, 595, 604, 704, 902, 916
BstNI CCWGG 1 cut(s) 1145
BstSCI CCNGG 1 cut(s) 1143
BstSLI GKGCMC 2 cut(s) 1048, 1174
BstUI CGCG 1 cut(s) 779
BstV1I GCAGC 2 cut(s) 3, 214
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
Bsu36I CCTNAGG 1 cut(s) 87
BsuRI GGCC 9 cut(s) 85, 92, 283, 368, 614, 677, 1013, 1028, 1253
BtgI CCRYGG 2 cut(s) 11, 711
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 3 cut(s) 515, 596, 1326
BveI ACCTGC 1 cut(s) 585
Cac8I GCNNGC 2 cut(s) 285, 483
CciI TCATGA 1 cut(s) 22
CfoI GCGC 2 cut(s) 481, 707
CseI GACGC 2 cut(s) 768, 785
Csp6I GTAC 3 cut(s) 274, 513, 1282
CviAII CATG 6 cut(s) 12, 23, 131, 279, 633, 719
CviQI GTAC 3 cut(s) 274, 513, 1282
DdeI CTNAG 3 cut(s) 87, 411, 992
EaeI YGGCCR 3 cut(s) 281, 612, 675
Eam1104I CTCTTC 3 cut(s) 183, 264, 894
EarI CTCTTC 3 cut(s) 183, 264, 894
Ecl136II GAGCTC 2 cut(s) 844, 919
Eco130I CCWWGG 4 cut(s) 11, 615, 984, 1292
Eco24I GRGCYC 2 cut(s) 846, 921
Eco31I GGTCTC 1 cut(s) 1049
Eco32I GATATC 1 cut(s) 1152
Eco53kI GAGCTC 2 cut(s) 844, 919
Eco57I CTGAAG 2 cut(s) 539, 918
Eco81I CCTNAGG 1 cut(s) 87
Eco88I CYCGRG 4 cut(s) 107, 1052, 1160, 1174
EcoICRI GAGCTC 2 cut(s) 844, 919
EcoRII CCWGG 1 cut(s) 1143
EcoRV GATATC 1 cut(s) 1152
EcoT14I CCWWGG 4 cut(s) 11, 615, 984, 1292
EcoT38I GRGCYC 2 cut(s) 846, 921
ErhI CCWWGG 4 cut(s) 11, 615, 984, 1292
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 6 cut(s) 15, 26, 134, 282, 636, 722
FaqI GGGAC 1 cut(s) 869
FatI CATG 6 cut(s) 11, 22, 130, 278, 632, 718
Fnu4HI GCNGC 3 cut(s) 17, 203, 675
FokI GGATG 1 cut(s) 398
FriOI GRGCYC 2 cut(s) 846, 921
Fsp4HI GCNGC 3 cut(s) 17, 203, 675
FspBI CTAG 2 cut(s) 851, 1350
GlaI GCGC 2 cut(s) 480, 706
GluI GCNGC 3 cut(s) 17, 203, 675
GsaI CCCAGC 1 cut(s) 328
GsuI CTGGAG 2 cut(s) 804, 1166
HaeII RGCGCY 1 cut(s) 708
HaeIII GGCC 9 cut(s) 85, 92, 283, 368, 614, 677, 1013, 1028, 1253
HapII CCGG 3 cut(s) 443, 646, 763
HgaI GACGC 2 cut(s) 768, 785
HhaI GCGC 2 cut(s) 481, 707
Hin1I GRCGYC 1 cut(s) 855
Hin1II CATG 6 cut(s) 15, 26, 134, 282, 636, 722
Hin6I GCGC 2 cut(s) 479, 705
HinP1I GCGC 2 cut(s) 479, 705
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 1307
HinfI GANTC 5 cut(s) 289, 649, 818, 834, 1215
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 3 cut(s) 443, 646, 763
HphI GGTGA 5 cut(s) 394, 566, 1111, 1219, 1333
Hpy166II GTNNAC 3 cut(s) 466, 515, 1284
Hpy188I TCNGA 6 cut(s) 335, 412, 531, 978, 993, 1008
Hpy8I GTNNAC 3 cut(s) 466, 515, 1284
Hpy99I CGWCG 2 cut(s) 779, 1025
HpyAV CCTTC 6 cut(s) 328, 517, 769, 955, 1090, 1096
HpyCH4III ACNGT 4 cut(s) 512, 712, 871, 1330
HpyCH4IV ACGT 1 cut(s) 855
HpyCH4V TGCA 4 cut(s) 4, 58, 149, 598
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 155, 199, 586, 595, 604, 704, 902, 916
HpyF3I CTNAG 3 cut(s) 87, 411, 992
HpySE526I ACGT 1 cut(s) 855
Hsp92I GRCGYC 1 cut(s) 855
Hsp92II CATG 6 cut(s) 15, 26, 134, 282, 636, 722
HspAI GCGC 2 cut(s) 479, 705
KspAI GTTAAC 1 cut(s) 466
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 3 cut(s) 7, 841, 916
Lsp1109I GCAGC 2 cut(s) 3, 214
LweI GCATC 2 cut(s) 67, 1309
MaeI CTAG 2 cut(s) 851, 1350
MaeII ACGT 1 cut(s) 855
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 5 cut(s) 846, 921, 1048, 1174, 1341
MlsI TGGCCA 2 cut(s) 283, 614
MluCI AATT 9 cut(s) 53, 94, 171, 217, 355, 668, 955, 1039, 1261
MluI ACGCGT 1 cut(s) 777
MluNI TGGCCA 2 cut(s) 283, 614
MlyI GAGTC 1 cut(s) 843
Mox20I TGGCCA 2 cut(s) 283, 614
MroXI GAANNNNTTC 1 cut(s) 1214
MscI TGGCCA 2 cut(s) 283, 614
MseI TTAA 7 cut(s) 174, 465, 726, 801, 954, 1119, 1127
Msp20I TGGCCA 2 cut(s) 283, 614
MspCI CTTAAG 1 cut(s) 800
MspI CCGG 3 cut(s) 443, 646, 763
MspR9I CCNGG 1 cut(s) 1145
MvaI CCWGG 1 cut(s) 1145
MvnI CGCG 1 cut(s) 779
MwoI GCNNNNNNNGC 9 cut(s) 16, 155, 199, 586, 595, 604, 704, 902, 916
NcoI CCATGG 1 cut(s) 11
NlaIII CATG 6 cut(s) 15, 26, 134, 282, 636, 722
NlaIV GGNNCC 3 cut(s) 9, 873, 1142
PaeR7I CTCGAG 3 cut(s) 107, 1052, 1160
PagI TCATGA 1 cut(s) 22
PaqCI CACCTGC 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 183
PcsI WCGNNNNNNNCGW 2 cut(s) 852, 1026
PdmI GAANNNNTTC 1 cut(s) 1214
PfeI GAWTC 4 cut(s) 289, 649, 818, 1215
PflMI CCANNNNNTGG 3 cut(s) 176, 694, 1293
PkrI GCNGC 3 cut(s) 18, 204, 676
PleI GAGTC 1 cut(s) 842
PpsI GAGTC 1 cut(s) 842
PshBI ATTAAT 1 cut(s) 174
Psp124BI GAGCTC 2 cut(s) 846, 921
Psp6I CCWGG 1 cut(s) 1143
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 1 cut(s) 1143
PspN4I GGNNCC 3 cut(s) 9, 873, 1142
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 3 cut(s) 275, 514, 1283
RsaNI GTAC 3 cut(s) 274, 513, 1282
SacI GAGCTC 2 cut(s) 846, 921
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 7 cut(s) 174, 465, 726, 801, 954, 1119, 1127
SatI GCNGC 3 cut(s) 17, 203, 675
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 1 cut(s) 843
ScrFI CCNGG 1 cut(s) 1145
SduI GDGCHC 5 cut(s) 846, 921, 1048, 1174, 1341
SfaNI GCATC 2 cut(s) 67, 1309
Sfr274I CTCGAG 3 cut(s) 107, 1052, 1160
SlaI CTCGAG 3 cut(s) 107, 1052, 1160
SmlI CTYRAG 8 cut(s) 107, 362, 800, 911, 920, 1052, 1160, 1361
SmoI CTYRAG 8 cut(s) 107, 362, 800, 911, 920, 1052, 1160, 1361
Sse9I AATT 9 cut(s) 53, 94, 171, 217, 355, 668, 955, 1039, 1261
SsiI CCGC 1 cut(s) 674
SspMI CTAG 2 cut(s) 851, 1350
SstI GAGCTC 2 cut(s) 846, 921
StyD4I CCNGG 1 cut(s) 1143
StyI CCWWGG 4 cut(s) 11, 615, 984, 1292
TaaI ACNGT 4 cut(s) 512, 712, 871, 1330
TaiI ACGT 1 cut(s) 858
TasI AATT 9 cut(s) 53, 94, 171, 217, 355, 668, 955, 1039, 1261
TatI WGTACW 2 cut(s) 273, 512
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 4 cut(s) 289, 649, 818, 1215
Tru1I TTAA 7 cut(s) 174, 465, 726, 801, 954, 1119, 1127
Tru9I TTAA 7 cut(s) 174, 465, 726, 801, 954, 1119, 1127
TscAI CASTG 3 cut(s) 522, 596, 1333
TseI GCWGC 2 cut(s) 16, 202
TspDTI ATGAA 4 cut(s) 11, 471, 504, 1109
TspGWI ACGGA 2 cut(s) 90, 416
TspRI CASTG 3 cut(s) 522, 596, 1333
Van91I CCANNNNNTGG 3 cut(s) 176, 694, 1293
Vha464I CTTAAG 1 cut(s) 800
VspI ATTAAT 1 cut(s) 174
XapI RAATTY 2 cut(s) 668, 1261
XbaI TCTAGA 1 cut(s) 850
XhoI CTCGAG 3 cut(s) 107, 1052, 1160
XmnI GAANNNNTTC 1 cut(s) 1214
XspI CTAG 2 cut(s) 851, 1350
ZraI GACGTC 1 cut(s) 856
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.